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| 1 | Glutamate and aspartate alleviate testicular/epididymal oxidative stress by supporting antioxidant enzymes and immune defense systems in boars显示文摘Several potential oxidative agents have damaging effects on mammalian reproductive systems.This study was conducted to investigate the effects of glutamate(Glu)and aspartate(Asp)supplementation on antioxidant enzymes and immune defense systems in the outer scrotum of boars injected with H2O2.A total of 24 healthy boars were randomly divided into 4 treatment groups:control(basal diet,saline-treated),H2O2(basal diet,H2O2-challenged outer scrotum(1 m L kg^–1 BW)),Glu(basal diet+2%Glu,H2O2-challenged),and Asp(basal diet+2%Asp,H2O2-challenged).Our results showed that both Glu and Asp supplementation improved testicular morphology and decreased the genital index in the H2O2-treated boars.Glu and Asp administration increased the antioxidant enzyme activities and affected the testicular inflammatory cytokine secretion but had no effect on sex hormone levels.Furthermore,the m RNA expression of CAT,Cu Zn SOD,and GPx4 was altered in the testes and epididymis of boars treated with Asp and Glu.Glu and Asp supplementation also modulated the expression of TGF-β1,IL-10,TNF-α,IL-6 and IL-1βin the testis and epididymis.These results indicate that dietary Glu and Asp supplementation might enhance antioxidant capacity and regulate the secretion and expression of inflammatory cytokines to protect the testes and epididymis of boars against oxidative stress. | Wenjie Tang Jian Wu Shunshun Jin Liuqin He Qinlu Lin Feijun Luo Xingguo He Yanzhong Feng Binsheng He Pingping Bing Tiejun Li Yulong Yin | 2020 | Science China(Life Sciences)2020，63，1： | 9 |
| 2 | Assessment of the immune responses to Treponema pallidum Gpd DNA vaccine adjuvanted with IL-2 and chitosan nanoparticles before and after Treponema pallidum challenge in rabbits显示文摘Syphilis is a multistage,sexually transmitted disease caused by the spirochete,Treponema pallidum(Tp).A significantly high incidence of syphilis has been reported in several countries,including China,and there is an urgent need for the development of efficacious vaccines against syphilis.DNA vaccines are a major breakthrough in the field of vaccination with several advantages over traditional vaccines.Animal model studies of Tp DNA vaccines have not been reported elsewhere but our previous reports describe the development of a single-gene Tp DNA vaccine and preclinical immunization study.In this study,chitosan(CS) nanoparticles were used as a vector and an interleukin-2 expression plasmid(pIL-2) as an adjuvant to enhance a TpGpd DNA vaccine candidate(pTpGpd) in a rabbit Tp skin challenge model.At week 8 after the first immunization,three rabbits from each group were used to determine cytokine measurements and spleen lymphocyte proliferation assay.pTpGpd in combination with pIL-2 wrapped with CS led to the greatest enhancement of anti-TpGpd antibodies and T-cell proliferation.During infection,levels of anti-TpGpd antibodies and T-cell proliferation were measured.Both the serum special IgG and IL-2,interferon-γ were significantly increased by the co-injection of the IL-2 plasmid compared with the injection of TpGpd DNA alone(P<0.05).Furthermore,IL-2 plasmid coinjection efficiently enhanced the antigen-specific lymphocyte proliferation response.Additionally,the ratios of positive skin lesions and ulcer lesions in groups immunized with pTpGpd were significantly lower than those of the pIL-2,CS or pIL-2 mixed with CS control groups(P<0.001).CS vectored and pIL-2 adjuvanted pTpGpd immunized animals exhibited the lowest rates of positive skin tests(8.33%) and ulcer lesions(4.17%) and the fastest recovery(42 d).These experiments indicate that co-injection of a pIL-2 plasmid with pTpGpd DNA vaccine wrapped with CS can significantly strengthen the long-term stability of immune response during infection,efficiently improve the protective effect against T.pallidum spirochetes infection and attenuate syphilitic lesion development. | ZHAO FeiJun ZHANG XiaoHong LIU ShuangQuan ZENG TieBing YU Jian GU WeiMing ZHANG YueJun CHEN Xi WU YiMou | 2013 | Science China(Life Sciences)2013，56，2： | 8 |
| 3 | Electrospinning of hydroxypropyl methylcellulose trimellitate solutions显示文摘Nonwoven fiber mats of hydroxypropyl methylcellulose trimellitate(HPMCT) with potential applications in controlled delivery of drugs and scaffolds for tissue cultures have been successfully fabricated by electrospinning of HPMCT solutions.The formation and diameters of HPMCT fibers fabricated by electrospinning were strongly influenced by the solvents employed,electrostatic field strength,and solution concentrations.The electrospun products generated from all HPMCT solutions with various weight-average molecular weights in methanol/dichloromethane(1:1) solvent consisted of clustered beads,while methanol/dichloromethane(1:5) solvent helped to generate smooth,uniform,submicron fibers.From the relationship between specific viscosity and molecular weight in different solvents,it could be concluded that methanol/dichloromethane(1:5) solvent enhanced the molecular entanglements,improved the viscosity of the solutions,thus helped to generate smooth fibers.When all the other variables were kept constant,the concentration range suitable for electrospinning was between 10% and 16%(wt),and the average fiber diameter increased as the concentration increased.When all the other variables were kept constant,the average fiber diameter decreased as the strength of the electrostatic field increased.Electrospinning the solutions of two compounds with similar structure,hydroxypropyl methylcellulose phthalate(HPMCP) and HPMCT,we found that the fibers electrospun from HPMCP were thicker than that from HPMCT,which is attributed to the fact that the solution's conductivity of HPMCT(containing two free carboxyl groups) is higher than that of HPMCP(containing only one free carboxyl group). | MA Gang,SHAO ZiQiang,WANG WenJun,WANG FeiJun,TAN LingLing & LIAO Bing School of Material,Beijing Institute of Technology,Beijing 100081,China | 2010 | Science China Chemistry2010，53，1： | 3 |
| 4 | EFFECT OF UNEQUAL DEFORMATION IN DEVELOPMENT OF ADVANCED PLASTIC PROCESSING TECHNOLOGIES显示文摘An effect of unequal deformation in development of advanced plastic processing technologies is researched by studying an in-plane bending process of strip metal under unequal compressing. The research results show the following: If appropriately controlled, unequal plastic deformation can play an important role not only in the improvement of quality of parts obtained by plastic processing technologies, but also in the development of new processes for advanced plastic working technologies. A coordinated growth of unequal plastic deformation can develop the deformation potentiality of material to the full. The degree of unequal plastic deformation can be used as bases for optimization design of processes and dies of plastic forming. | Yang He Xian Feijun College of Materials Science and Engineering,Northwestern Polytechnical University,Xi’an 710072,China | 2002 | Chinese Journal of Mechanical Engineering2002，15，1： | 3 |
| 5 | Development and Prospects for Energy Saving Technology in Oil & Gas Fields,China显示文摘Current state of energy saving technology in China's oil and gas fields System optimization To optimize the oil-gas field surface engineering system is critical to improve the efficiency of oil and gas field system.To adapt to the changes in development of old oil and gas fields,all oilfields are adjusted and reconstructed;a set of optimized and simplified modes and technical measures are developed. | Chen Youwang Yu Jiqing Lin Ran Zhu Yingru Liu Feijun | 2010 | China Oil & Gas2010，17，4： | 2 |
| 6 | Separation of injectable salidroside by column chromatography of macroporous resins for treating myocardial ischemia显示文摘The objective of the present study is to develop a method for large-scale separating and purifying salidroside from rhodiola kirilowii roots and for preparing injectable medicinal ingredient.Crude extract of salidroside was prepared by water-ethanol system,and purified by column chromatography of macroporous resins.Static adsorption and desorption studies were performed on six kinds of macroporous resins,and SP825 resin was chosen,followed by optimizing process parameters.The optimum sample volume,feed concentration,ratio of diameter to height,and feeding flow rate were 1.5 bed volumes(BV),15 mg/mL,1:10 and 1 BV/h,respectively.Dynamic desorption was performed consecutively with 8 BV of distilled water,3 BV of 5% ethanol and 8 BV of 10% ethanol at a flow rate of 2 BV/h.After three cycles in separating 3.5 tons of rhodiola kirilowii roots,salidroside purity was increased from 3.4% in the crude extract to 93.6% in purified salidroside product.This study provides a novel method to separate salidroside for injectable use. | JU RuiJun HUANG RenJie ZHOU Jia LI RuoJing ZHOU Peng ZHANG ZaoHua XIANG FeiJun XU DongJin LIU WeiXiang MA XingTian ZHANG Qiang LU WanLiang | 2012 | Science China Chemistry2012，55，7： | 2 |
| 7 | Natural alleles of a uridine 5'-diphospho-glucosyltransferase gene responsible for differential endosperm development between upland rice and paddy rice显示文摘Traditional upland rice generally exhibits insufficient grains resulting from abnormal endosperm development compared to paddy rice. However, the underlying molecular mechanism of this trait is poorly understood. Here,we cloned the uridine 5’-diphospho(UDP)-glucosyltransferase gene EDR1(Endosperm Development in Rice) responsible for differential endosperm development between upland rice and paddy rice by performing quantitative trait loci analysis and map-based cloning. EDR1 was highly expressed in developing seeds duringgrain filling. Natural variations in EDR1 significantly reduced the UDP-glucosyltransferase activity of EDR1 YZNcompared to EDR1 YD1,resulting in abnormal endosperm development in the near-isogenic line, accompanied by insufficient grains and changes in grain quality.By analyzing the distribution of the two alleles EDR1 YD1 and EDR1 YZNamong diverse paddy rice and upland rice varieties, we discovered that EDR1 was conserved in upland rice, but segregated in paddy rice. Further analyses of grain chalkiness in the alleles of EDR1 YD1 and EDR1 YZNvarieties indicated that rice varieties harboring EDR1 YZNand EDR1 YD1 preferentially showed high chalkiness, and low chalkiness,respectively. Taken together, these results suggest that the UDP-glucosyltransferase gene EDR1 is an important determinant controlling differential endosperm development between upland rice and paddy rice. | Zihao Wu Xiao Zhang Guimei Chang Jun Yang Jinpeng Wan Feijun Wang Dayun Tao Jiawu Zhou Lianguang Shang Peng Xu Diqiu Yu | 2022 | Journal of Integrative Plant Biology2022，64，1： | 1 |
| 8 | Identification of aberrantly expressed miRNAs in rectal cancer 显示文摘 | LI XINHUA ZHANG GUIYING LUO FEIJUN | 2012 | Oncol Rep2012，28，1： | 1 |
| 9 | Interference effect of epigallocatechin-3-gallate on targets of nuclear factor kappaB signal transduction pathways activated by EB virus encoded latent membrane protein1显示文摘 | Yan Z YongGuang T FeiJun L | 2004 | Int J Biochem Cell Biol2004，36，8： | 1 |
| 10 | The transcriptional transactivation function of HBx protein is important for its augmentation role in hepatitis B virus replication显示文摘 | HONG T LUVSANJAV D FEIJUN H | 2005 | J Virol2005，79，： | 1 |
| 11 | Identification of aberrantly expressed miRNAs in rectal cancer显示文摘 | Xinhua Li Guiying Zhang Feijun Luo Jinde Ruan Damao Huang Deyun Feng Desheng Xiao Zhijun Zeng Xiong Chen Wei Wu | 2012 | Oncology Reports2012，，： | 1 |
| 12 | Modeling and simulation of autonomous underwater vehicles:design and implementation显示文摘 | Song Feijun An P E Folleco A | 2003 | IEEE Journal of Oceanic Engineering2003，28，2： | 1 |
| 13 | Identification of new aberrantly expressed miRNAs in intestinal-typegastric cancer and its clinical significance显示文摘 | Xinhua Li Feijun Luo Qian Li Meihua Xu Deyun Feng Guiying Zhang Wei Wu | 2011 | Oncology Reports2011，，6： | 1 |
| 14 | Octacosanol and health benefits:Biological functions and mechanisms of action显示文摘Octacosanol is a widely distributed natural higher aliphatic alcohol that can be isolated and purified from rice bran,sugarcane,beeswax,insect wax,etc.Octacosanol exerts various biological effects,including anti-fatigue,anti-hypoxia,antioxidant,anti-inflammatory,antitumor,etc.Meanwhile,it has the effects of regulating the body's immune function and energy metabolism and has potential benefits for cardiovascular disease,cerebrovascular disorders,diabetes,Parkinson's disease,and others.Octacosanol is primarily responsible for regulating multiple signaling pathways,such as AMPK,PI3K/Akt,and MAPK/NF-κB,to achieve different physiological functions.This review systemically summarized the progress in characterization,extraction and purification,biological functions,molecular mechanisms,and bioavailability of octacosanol.This study will provide a reference for many investigators to further explore the physiological functions of higher aliphatic alcohols and apply them as supplements in functional foods. | Yaping Zhou Fuliang Cao Feijun Luo Qinlu Lin | 2022 | Food Bioscience2022，47，3： | 1 |
| 15 | Brown rice:a missing nutrient-rich health food显示文摘Brown rice(BR)is a traditional health food being rich in various active substances, which have effective preventive and therapeutic effects on many diseases. In this review, we systemically summarized the efficacy of BR on cardiovascular diseases(CVDs), hyperlipidemia, hypertension, diabetes, obesity, osteoporosis, neurodegenerative diseases, cancer, immune disorders, inflammation, anxiety, liver and stomach damage, etc. Underlying mechanisms have also been analyzed, which include regulating lipid synthesis and metabolism, improving insulin levels and increasing glucose metabolism, anti-oxidation, promoting the immune system, transcription and non-transcriptional regulation, blocking cell cycle, thereby improving disease symptoms and reducing its recurrence rate. Generally, BR has been almost disappeared in modern society, but it is promising to exploit the potential to become a functional food. Further research on the nutritional value, variety differences, storage problems, taste and molecular mechanism of BR will be conducive to help meticulously design clinical intervention trials and ultimately improve the treatment and prevention of various diseases. | Xiuxiu Wu Tianyi Guo Feijun Luo Qinlu Lin | 2023 | Food Science and Human Wellness2023，12，5： | 1 |
| 16 | Anticancer properties of Monascus metabolites显示文摘 | Tao Yang Junwen Liu Feijun Luo Qinlu Lin Thomas J. Rosol Xiyun Deng | 2014 | Anti-Cancer Drugs2014，，7： | 1 |
| 17 | Solid-state red emission of boron-fluorine complexes with extendedrr-conjugated structure 显示文摘 | Liang Y Feijun D Wenjun L | 2013 | J Fluorine Chem2013，156，： | 1 |
| 18 | Identification of new aberrantly expressed miRNAs in intestinal-typegastric cancer and its clinical significance显示文摘 | Xinhua Li Feijun Luo Qian Li Meihua Xu Deyun Feng Guiying Zhang Wei Wu | 2011 | Oncology Reports2011，，6： | 1 |
| 19 | STAT3 activation induced by Epstein-Barr virus latent membrane protein1 causes vascular endothelial growth factor expression and cellular invasiveness via JAK3 And ERK signaling显示文摘 | Zhenlian Wang Feijun Luo Lili Li Lifang Yang Duosha Hu Xiaoqian Ma Zhongxin Lu Lunquan Sun Ya Cao | 2010 | European Journal of Cancer2010，，16： | 1 |
| 20 | Identification and fine mapping of rtms1-D,a gene responsible for reverse thermosensitive genic male sterility from Diannong S-1X显示文摘Thermosensitive genic male sterility(TGMS)has been widely used in two-line hybrid rice breeding.Due to hybrid seed production being highly affected by changeable environments,its application scope is limited to some extent.Thus,it is of great importance to identify potential TGMS genes in specific rice varieties.Here,Diannong S-1 xuan(DNS-1 X),a reverse TGMS(RTGMS)japonica male sterile line,was identified from Diannong S-1.Genetic analysis showed that male sterility was tightly controlled by a single recessive gene,which was supported by the phenotype of the F;and F;populations derived from the cross between DNS-1 X and Yunjing 26(YJ26).Combining simple sequence repeat(SSR)markers and bulked segregation analysis(BSA),we identified a 215 kb region on chromosome 10 as a candidate reverse TGMS region,which was designated as rtmsl-D.It was narrower than the previously reported RTGMS genes rtmsl and tms6(t).The fertility conversion detected in the natural environment showed that DNS-1 X was sterile below 28-30℃;otherwise,it was fertile.Histological analysis further indicated that the pollen abortion was occurred in the young microspore stage.This study will provide new resources for two-line hybrid rice and pave the way for molecular breeding of RTGMS lines. | Xiao Zhang Guimei Chang Zihao Wu Jinpeng Wan Jun Yang Feijun Wang Fang Wang Diqiu Yu Peng Xu | 2022 | Plant Diversity2022，44，2： | 0 |
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