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| 1 | The wound healing potential of collagen peptides derived from the jellyfish Rhopilema esculentum显示文摘Purpose: Wound represents a major health challenge as they consume a large amount of healthcare resources to improve patient's quality of life. Many scientific studies have been conducted in search of ideal biomaterials with wound-healing activity for clinical use and collagen has been proven to be a suitable can didate biomaterial. This study in tended to investigate the wound healing activity of collagen peptides derived from jellyfish following oral administration. Methods: In this study, collagen was extracted from the jellyfish-Rhopilema esculentum using 1% pepsin. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and fourier transform infrared (FTIR) were used to identify and determine the molecular weight of the jellyfish collagen. Collagenase II, papain and alkaline proteinase were used to breakdown jellyfish collagen into collagen peptides. Wound scratch assay (in vitro) was done to determine migration potential of human umbilical vein endothelial cells (HUVEC) covering the artificial wound created on the cell monolayer following treatment with collagen peptides. In vivo studies were con ducted to determine the effects of collagen peptides on wound healing by examining wound contraction, re-epithelialization, tissue regeneration and collagen deposition on the wounded skin of mice. Confidence level (p < 0.05) was considered significant using GraphPad Prism software. Results: The yield of collagen was 4.31%. The SDS-PAGE and FTIR showed that extracted collagen from jellyfish was type I. Enzymatic hydrolysis of this collagen using collagenase II produced collagen peptides (CPi) and hydrolysis with alkaline proteinase/papain resulted into collagen peptides (CP2). Tricine SDSPAGE revealed that collagen peptides consisted of protein fragments with molecular weight <25 kDa. Wound scratch assay showed that there were significant effects on the scratch closure on cells treated with collagen peptides at a concentration of 6.25 μg/mL for 48 h as compared to the vehicle treated cells. Overall treatment with collagen peptide on mice with full thickness excised wounds had a positive result in wound contraction as compared with the control. Histological assessment of peptides treated mice models showed remarkable sign of re-epithelialization, tissue regeneration and increased collagen deposition. Immunohistochemistry of the skin sections showed a significant increase in β-fibroblast growth factor (β-FGF) and the transforming growth factor-βi (TGF-βi) expression on collagen peptides treated group. Conclusion: Collagen peptides derived from the jellyfish-Rhopilema esculentum can accelerate the wound healing process thus could be a therapeutic potential product that may be beneficial in wound clinics in the future. | Fatuma Felix Felician Rui-He Yu Meng-Zhen Li Chun-Jie Li Hui-Qin Chen Ying Jiang Tao Tang Wei-Yan Qi Han-Mei Xu | 2019 | Chinese Journal of Traumatology2019，22，1： | 7 |
| 2 | Posterobasal left ventricular aneurysm after myocardial infarction with normal coronary arteries:Case-report显示文摘We present a case of a 64-year-old woman with signs of debilitating condition including anginal chest pain, exertional dyspnea, and depression. The patient had previously suffered from a myocardial infarction after a loss of a close family member. Workup showed a posterobasal left ventricular aneurysm and moderate to severe mitral regurgitation in the absence of coronary atherosclerosis. Routine ultrasonography revealed abdominal aortic aneurysm and intraabdominal aortic deviation. The patient was immediately started on optimal medical treatment. On repeat assessment general condition was satisfactory, vital signs were normal, and investigations showed no signs of progressive heart failure or other significant clinical changes. Although prognosis in patients with myocardial infarction with normal coronary arteries is generally considered favorable, mechanical complications such as posterobasal left ventricular aneurysm with moderate to severe mitral regurgitation are possible. | Roman Evgenyevich Kalinin Igor Aleksandrovich Suchkov Nina Dzhansugovna Mzhavanadze Adelphina Felician Ncheye | 2018 | World Journal of Clinical Cases2018，6，6： | 2 |
| 3 | Colostong and drainage for cinlian rectal injuries:is that all显示文摘 | Burch Jm Felician DV Mattox KI | 1989 | Ann Surg1989，209，： | 1 |
| 4 | The neurobiology and pharmacotherapy of Alzheimer's disease 显示文摘 | Felician O Sandson TA | 1999 | J Neuropsyehiatry Clin Neurosci1999，11，： | 1 |
| 5 | The neurobiology and pharmacotherapy of Alzhelmer's disease显示文摘 | Felician O Sandson TA | 1999 | J Neuropsychiatry Clin Neurosci1999，11，： | 1 |
| 6 | Coronary artery blood flow:physiology and pathophysiologic regulation显示文摘 | | 1999 | Clin Cardiol1999，22，： | 1 |
| 7 | The role of area 17 in visual imagery: convergent evidence from PET and rTMS 显示文摘 | Kosslyn SM Pascuai-Leone A Felician O | 1999 | Sci1999，284，5411： | 1 |
| 8 | The role of area 17in visual imagery: convergen evidence from pet and rTMS 显示文摘 | Kosslyn SM Pascual-leone A Felician O | 1999 | Sci-ence1999，284，5411： | 1 |
| 9 | Diffusionweighted magnetic resonance imaging in Alzheimer's disease显示文摘 | Sandson TA Felician O Edelman RR | | 0，，02： | 1 |
| 10 | Management and short- term patency of lower extremity venous injuries with various repairs显示文摘 | Parry NG Felicians DV Burke RM | 2003 | Am J Surg2003，186，6： | 1 |
| 11 | Impaired configurational processing in a case of progressive prosopagnosia associated with predominant right temporal lobe atrophy显示文摘 | Joubert S Felician O Barbeau E | 2003 | Brain2003，126，： | 1 |
| 12 | The role of area 17 in visual imagery: convergent evidence from pet and rTMS显示文摘 | Kosslyn SM Pascual-Leone A Felician O | 1999 | Science1999，284，5411： | 1 |
| 13 | The neuro-biology and pharmacotherapy of Alzheimer’s disease显示文摘 | Felician O Sandson TA | 1999 | J Neuropsychiatry & Clin Neurosci1999，11，1： | 1 |
| 14 | The neurobiology and pharmacotherapy of Azheimer's disease显示文摘 | Felician O Sandson TA | 1999 | J Neuropsychiatry ClinNeurosci1999，11，： | 1 |
| 15 | Using wavelet de-noised spectra in NMRscreening显示文摘 | NIKOLA T FELICIAN D | 2005 | Journal of MagneticResonance2005，173，： | 1 |
| 16 | Development and evaluation of nifedipine controlled release tablets prepared using mucoadhesive swellable polymer显示文摘 | W. A. Felician E. S. Ghaly | 2011 | Pharmaceutical Development and Technology2011，，5： | 1 |
| 17 | Functional connectivity- changes differ in early and late-onset Alzheirner's dis- ease显示文摘 | Gour N Felician O Didic M et a/ | 2014 | I-Ium Brain Mapp2014，35，7： | 1 |
| 18 | The neurobiology and pharmacotherapy of Alzheimer′s disease显示文摘 | Felician O Sandson TA | 1999 | J Neuropsychiatry Clin Neurosci1999，11，： | 1 |
| 19 | The role of area 17 in visual imagery:convergent evidence from PET and rTMS显示文摘 | Kosslyn SM Pascual-Leone A Felician O | 1999 | Science1999，284，： | 1 |
| 20 | 图象库管理系统——大型办公室自动化系统环境的有力工具显示文摘目前,数据库系统正面临一个新的应用领域——图象库系统。一“幅图象”实际上是被数字化了的按位串方式存贮的文件页。它在办公室自动化方面的应用包括文件归档,大型多媒体数据库,图书、情报资料存贮和检索;在科技和医学方面的应用包括卫星、地质和人体扫描图象的记录和存贮。本文介绍了在大型磁盘和光盘上图象库管理系统(IBMS)的体系结构方面的需求,并指出由光盘的不可删除性引出的问题。还详细讨论了图象库系统的数据结构和主要算法。 | Leonardo Felician 余宏华 马玉书 | 1991 | 计算机工程与应用1991，27，4： | 0 |
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