|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Black soldier fly and gut health in broiler chickens:insights into the relationship between cecal microbiota and intestinal mucin composition显示文摘Background:The relationship between diet and intestinal microbiota and mucin composition appears to be fundamental for poultry gut health.The effects of insect meal(whose role as alternative feed ingredient is now well recognized)on gut microbiota and mucin composition have recently been reported in Tenebrio molitor-fed free-range and broiler chickens,but no data are currently available for Hermetia illucens(HI)-fed broilers.The present study evaluated the effects of dietary HI meal inclusion on cecal microbiota and intestinal mucin composition of broiler chickens.Results:A total of 256 male broiler chickens were allotted to 4 dietary treatments(control diet[C]and 5%,10%and 15%HI meal inclusion,with 8 replicate pens/treatment and 8 birds/pen)and slaughtered at 35 d of age(2 animals/pen,16 birds/diet).The cecal microbiota assessment by 16S rRNA amplicon based sequencing showed lower alpha diversity in HI15 chickens(Shannon,P<0.05)and higher beta diversity(Adonis and ANOSIM,P<0.001)in birds fed HI diets than C.Furthermore,HI15 birds displayed significant increase of the relative abundance of Proteobacteria phylum(False Discovery Rate[FDR]<0.05)when compared to HI10.L-Ruminococcus(Ruminococcus from Lachnospiraceae family),Faecalibacterium,Blautia and Clostridium genera were found to be characteristic of HI5 cecal microbiota(FDR<0.05),while broiler chickens fed HI10 and HI15 diets were characterized(FDR<0.05)by Lactobacillus and Ruminococcus(HI10)and Bacteroides,Roseburia and Helicobacter genera(HI15).Periodic-acid Schiff,Alcian Blue pH 2.5 and high iron diamine staining on small and large intestine also demonstrated lower mucin staining intensity in the intestinal villi of HI10 and HI15 birds than C(P<0.05).Conclusions:Dietary HI meal utilization at low inclusion levels(i.e.,5%)positively influenced either the cecal microbiota or the gut mucin dynamics in terms of selection of potentially beneficial bacteria and increase in villi mucins.However,high inclusion levels(in particular the 15%)may have a negative influence in terms of partial reduction of microbial complexity,reduction of potentially beneficial bacteria,selection of bacteria with mucolytic activity and decrease in villi mucins. | Ilaria Biasato Ilario Ferrocino Sihem Dabbou Rocchina Evangelista Francesco Gai Laura Gasco Luca Cocolin Maria Teresa Capucchio Achille Schiavone | 2020 | Journal of Animal Science and Biotechnology2020，11，3： | 4 |
| 2 | Molecular identification of mesophilic and psychrotrophic bacteria from raw cow's milk显示文摘 | ERCOLINI D RUSSO F FERROCINO I | 2009 | Food microbiology2009，26，2： | 1 |
| 3 | Development of spoilage raicmbiota in beef stored in nisin activated packaging显示文摘 | Ercolini D Ferrocino I la Storia A | 2010 | Food Mi- crobiolagy2010，27，1： | 1 |
| 4 | Spoilage-related activity of Carnobacterium maltaromaticum strains in air-stored and vacuum-packed meat显示文摘 | Casaburi A Nasi A Ferrocino I | 2011 | Applied and environmental microbiology2011，77，20： | 1 |
| 5 | Antimierobial packaging to retard the growth of spoilage bacteria and to reduce the release of volatile metabolites in meat stored under vacuum at 1 ℃显示文摘 | Ferrocino I La Storia A Torrieri E | 2013 | Journal of Food Protection2013，76，1： | 1 |
| 6 | Development of spoilage microbiota in beef stored in nisin activated packaging显示文摘 | ERCOLINI D FERROCINO I LA STORIA A | 2010 | Food microbiology2010，27，1： | 1 |
| 7 | Molecular identification of mesophilic and psychrotrophic bacteria from raw cow's milk显示文摘 | ERCOLINI D RUSSO F FERROCINO I | 2009 | Food Microbiology2009，26，2： | 1 |
| 8 | Molecularidentification of mesophilic and psychrotrophic bacteria from rawcow’s milk显示文摘 | ERCOLINI D RUSSO F FERROCINO I | 2009 | Food Microbiology2009，26，2： | 1 |
| 9 | Development of spoilage microbiota in beef stored in nisin activated packaging显示文摘 | Ercolini D Ferrocino I La Storia A | 2010 | Food Microbiology2010，27，1： | 1 |
| 10 | Development of spoilage mierobiota in beef stored in nisin activated packaging 显示文摘 | Ercolini D Ferrocino I La Storia A | 2010 | Food Mierobiol2010，27，1： | 1 |
| 11 | Molecular identification of mesophilic and psychrotrophic bacteria from raw cowl milk 显示文摘 | Ercolini D Russo F Ferrocino I | 2009 | Food Microbiology2009，26，2： | 1 |
| 12 | Development of spoil-age microbiota in beef stored in nisin activated packaging显示文摘 | Ercolini D Ferrocino I LaStoria A | 2010 | Food Microbiology2010，27，1： | 1 |
| 13 | Molecularidentification of mesophilic and psychrotrophic bacteria from rawcow’s milk显示文摘 | Eroclini D Russo F Ferrocino I | 2009 | Food Microbiology2009，，26： | 1 |
| 14 | Development of spoilage microbiota in beef stored in nisin activated packaging显示文摘 | Danilo Ercolini Ilario Ferrocino Antonietta La Storia Gianluigi Mauriello Sergio Gigli Paolo Masi Francesco Villani | 2009 | Food Microbiology2009，，1： | 1 |
| 15 | Effects of dietary Hermetia illucens meal inclusion on cecal microbiota and small intestinal mucin dynamics and infiltration with immune cells of weaned piglets显示文摘Background: The constant interaction between diet and intestinal barrier has a crucial role in determining gut health in pigs. Hermetia illucens(HI) meal(that represents a promising, alternative feed ingredient for production animals) has recently been demonstrated to influence colonic microbiota, bacterial metabolite profile and mucosal immune status of pigs, but no data about modulation of gut mucin dynamics are currently available. The present study evaluated the effects of dietary HI meal inclusion on the small intestinal mucin composition of piglets, as well as providing insights into the cecal microbiota and the mucosal infiltration with immune cells.Results: A total of 48 weaned piglets were randomly allotted to 3 dietary treatments(control diet [C] and 5% or10% HI meal inclusion [HI5 and HI10], with 4 replicate boxes/treatment and 4 animals/box) and slaughtered after61 days of trial(3 animals/box, 12 piglets/diet). The cecal microbiota assessment by 16 S r RNA amplicon based sequencing showed higher beta diversity in the piglets fed the HI-based diets than the C(P < 0.001). Furthermore,the HI-fed animals showed increased abundance of Blautia, Chlamydia, Coprococcus, Eubacterium, Prevotella,Roseburia, unclassified members of Ruminococcaceae, Ruminococcus and Staphylococcus when compared to the C group(FDR < 0.05). The gut of the piglets fed the HI-based diets showed greater neutral mucin percentage than the C(P < 0.05), with the intestinal neutral mucins of the HI-fed animals being also higher than the sialomucins and the sulfomucins found in the gut of the C group(P < 0.05). Furthermore, the piglets fed the HI-based diets displayed lower histological scores in the jejunum than the other gut segments(ileum [HI5] or ileum and duodenum [HI10], P < 0.05).Conclusions: Dietary HI meal utilization positively influenced the cecal microbiota and the small intestinal mucin dynamics of the piglets in terms of selection of potentially beneficial bacteria and preservation of mature mucin secretory architecture, without determining the development of gut inflammation. These findings further confirm the suitability of including insect meal in swine diets. | Ilaria Biasato Ilario Ferrocino Elena Colombino Francesco Gai Achille Schiavone Luca Cocolin Valeria Vincenti Maria Teresa Capucchio Laura Gasco | 2020 | Journal of Animal Science and Biotechnology2020，11，4： | 1 |
| 16 | Development of spoilage microbiota in beef stored in nisin activated packaging显示文摘 | ERCOLINI D FERROCINO I LA STORIA A | 2010 | Food Microbiology2010，27，1： | 1 |
| 17 | Molecular identification of mesophilic and psychrotrophie bacteria from raw cow's milk 显示文摘 | Ercolini D Russo F Ferrocino I | 2009 | Food Microbiol2009，26，： | 1 |
| 18 | Dried fruit pomace inclusion in poultry diet:growth performance, intestinal morphology and physiology显示文摘Background: Fruit pomaces are by-products rich in polyphenol compounds and dietary fiber. They seem to play an important role in regulating the gut microbiota, morphology and physiology. The aim of this study was to assess whether apple(A), blackurrant(B) or strawberry(S) pomaces could be suitable ingredients in broiler diets and their effect on gut health. A total of 480 male broilers were randomly allotted to 8 dietary treatments with lower(3%-L) or higher(6%-H) dietary fiber content: two control groups(CL/CH), two A diets(AL/AH), two B diets(BL/BH), two S diets(SL/SH). Diet and fruit pomaces were chemically analyzed to assess polyphenol concentration and fibre fraction content. After the evaluation of growth performance, 6 birds/group were slaughtered at 35 days of age. Morphometric and histopathological investigations were performed on duodenum, jejunum and ileum.Excreta were collected to perform microbiota evaluation by 16 S DNA sequencing. Weight, viscosity, enzymatic activity, short chain fatty acid(SCFAs) and ammonia concentration were determined in ileum and/or ceca content.Results: A pomace and A diets showed the lowest polyphenol content and the highest content of soluble fibre fraction. No significant differences were observed for growth performance, gut morphometry and histopathology(P > 0.05). Dietary fruit pomace inclusion increased the weight of ileum and ceca and the ileum digesta viscosity(P < 0.05). In the ileum, A and S groups showed lower bacterial α-glucosidase activity than C groups. Moreover,small intestine SCFAs concentration was higher in fruit pomaces diets(P < 0.05). In ceca, B and S groups showed lower ammonia concentration and higher SCFAs than C. Dietary treatments also influenced the activity of α-glucosidase, α-galactosidase, β-galactosidase β-glucuronidase and xylase. Regarding microbiota, at phylum level,Firmicutes were differentially abundant across treatment(maximum for C and minimum in S, FDR > 0.05). At genus level, an increase of Weissella in AH and Erwinia in S/B diets, as well as a decrease of Lactobacillus in all fruit pomace groups were recorded(P < 0.05).Conclusions: Fruit pomaces could be suitable ingredients in poultry nutrition even if further studies are needed to better understand which doses is more recommended to avoid negative effects on gut microbiota. | Elena Colombino Ilario Ferrocino Ilaria Biasato Luca Simone Cocolin Daniel Prieto-Botella Zenon Zduńczyk Jan Jankowski Joanna Milala Monika Kosmala Bartosz Fotschki Maria Teresa Capucchio Jerzy Juśkiewicz | 2020 | Journal of Animal Science and Biotechnology2020，11，4： | 1 |
| 19 | Development ofspoilage microbiota in beef stored in nisin activated packaging显示文摘 | Ercolini D Ferrocino I Storia AL | 2010 | Food Microbiology2010，27，1： | 1 |
| 20 | Development of spoilage microbiota in beef stored in nisin activated packaging显示文摘 | ERCOLINI D FERROCINO I STORIA A L | 2010 | Food Microbiology2010，27，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员