|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Inhibitory effect of schisandrin B on free fatty acid-induced steatosis in L-02 cells显示文摘AIM:To investigate the effects of schisandrin B (Sch B) on free fatty acid (FFA)-induced steatosis in L-02 cells.METHODS:Cellular steatosis was induced by incubating L-02 cells with a FFA mixture (oleate and palmitate at the ratio of 2:1) for 24 h.Cytotoxicity and apoptosis were evaluated by 3-(4,5-dmethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and Annexin V/propidium iodide staining,respectively.Cellular total lipid was determined using a photocolorimetric method after Nile red staining,and triglyceride content was measured using an enzymatic kit.To study the effects of Sch B on steatosis,L-02 cells were treated with Sch B (1-100 μmol/L) in the absence or presence of 1 mmol/L FFA for 24 h,and cellular total lipid and triglyceride levels were measured.To explore the mechanisms of action of Sch B in the steatotic L-02 cells,mRNA levels of several regulators of hepatic lipid metabolism including adipose differentiation related protein (ADRP),sterol regulatory element binding protein 1 (SREBP-1),peroxisome proliferator-activated receptor (PPAR)-α and PPAR-γ were measured by quantitative real-time polymerase chain reaction (PCR),and protein levels of ADRP and SREBP-1 were measured by immunoblotting.RESULTS:Treatment with 1 mmol/L FFA for 24 h induced intracellular lipid accumulation in L-02 cells comparable to that in human steatotic livers without causing apparent apoptosis and cytotoxicity.Sch B mitigated cellular total lipid and triglyceride accumulations in the steatotic L-02 cells in a dose-dependent manner.Quantitative real-time PCR and Western blot analyses revealed that treatment of L-02 cells with 100 μmol/L Sch B reverted the FFA-stimulated up-regulation of ADRP and SREBP-1.CONCLUSION:Sch B inhibits FFA-induced steatosis in L-02 cells by,at least in part,reversing the up-regulation of ADRP and SREBP-1. | Jian-Hong Chu Hui wang yan ye Ping-Kei Chan Si-Yuan Pan Wang-Fun Fong Zhi-Ling Yu | 2011 | World Journal of Gastroenterology2011，17，19： | 4 |
| 2 | Projection of the Zhujiang(Pearl) River Delta's potential submerged area due to sea level rise during the 21st century based on CMIP5 simulations显示文摘Projections of potential submerged area due to sea level rise are helpful for improving understanding of the influence of ongoing global warming on coastal areas. The Ensemble Empirical Mode Decomposition method is used to adaptively decompose the sea level time series in order to extract the secular trend component. Then the linear relationship between the global mean sea level(GMSL) change and the Zhujiang(Pearl) River Delta(PRD)sea level change is calculated: an increase of 1.0 m in the GMSL corresponds to a 1.3 m(uncertainty interval from1.25 to 1.46 m) increase in the PRD. Based on this relationship and the GMSL rise projected by the Coupled Model Intercomparison Project Phase 5 under three greenhouse gas emission scenarios(representative concentration pathways, or RCPs, from low to high emission scenarios RCP2.6, RCP4.5, and RCP8.5), the PRD sea level is calculated and projected for the period 2006–2100. By around the year 2050, the PRD sea level will rise 0.29(0.21 to 0.40) m under RCP2.6, 0.31(0.22 to 0.42) m under RCP4.5, and 0.34(0.25 to 0.46) m under RCP8.5, respectively.By 2100, it will rise 0.59(0.36 to 0.88) m, 0.71(0.47 to 1.02) m, and 1.0(0.68 to 1.41) m, respectively. In addition,considering the extreme value of relative sea level due to land subsidence(i.e., 0.20 m) and that obtained from intermonthly variability(i.e., 0.33 m), the PRD sea level will rise 1.94 m by the year 2100 under the RCP8.5scenario with the upper uncertainty level(i.e., 1.41 m). Accordingly, the potential submerged area is 8.57×103 km2 for the PRD, about 1.3 times its present area. | XIA Jiangjiang YAN Zhongwei ZHOU Wen FONG Soi Kun LEONG Ka Cheng TANG Iu Man CHANG S W LEONG W K JIN Shaofei | 2015 | Acta Oceanologica Sinica2015，34，9： | 3 |
| 3 | Selection and optimization of background electrolytes for simultaneous detection of small cations and organic acids by capillary electrophoresis with indirect photometry显示文摘 | Xiong Xiang Li Sam Fong Yan | 1998 | Journal of chromatography a1998，822，： | 1 |
| 4 | A kinematic approach for eliminating the discontinuity of motion characteristics of cam-follower systems 显示文摘 | Yan H S Hsu M H Fong M K | 1994 | J Appl Mech Robot1994，1，2： | 1 |
| 5 | 1α,25(OH) 2 -Dihydroxyvitamin D 3 /VDR protects the skin from UVB-induced tumor formation by interacting with the β-catenin pathway显示文摘 | Yan J. Jiang Arnaud E. Teichert Frankie Fong Yuko Oda Daniel D. Bikle | 2013 | Journal of Steroid Biochemistry and Molecular Biology2013，，： | 1 |
| 6 | English in China:some thoughts after the Beijing Olympics 显示文摘 | Emily Tsz Yan Fong | 2009 | English Today2009，，1： | 1 |
| 7 | Health Problems Encountered by Dying Patients Receiving Palliative Home Care Until Death显示文摘 | Frances KYW Chun Fong Liu Yan Szeto | 2004 | Cancer Nursing2004，27，3： | 1 |
| 8 | POCS-Based Blocking Artifacts Suppression Using a Smoothness Constraint Set With Explicit Region Modeling显示文摘 | Alan Wee - Chung Liew Hong Yan and Ngai - Fong Law | 2005 | IEEE TRANSACTIONS ON CIRCUITS AND SYSTEMS FOR VIDEO TECHNOLOGY2005，15，6： | 1 |
| 9 | Effects of post annealing treatments on the characteristics of ohmic contacts on n-type A1GaN 显示文摘 | Zainuriah Hassan Fong Kwong Yam Yan Cheung Lee | 2005 | SPIE2005，5739，： | 1 |
| 10 | Natural history and clinical predictors of clinical progression in benign prostatic hyperplasia显示文摘 | Yan Kit Fong Shirin Milani Bob Djavan | 2005 | Current Opinion in Urology2005，，1： | 1 |
| 11 | Blocking Neuropilin-1 Function Has an Additive Effect with Anti-VEGF to Inhibit Tumor Growth显示文摘 | Qi Pan Yvan Chanthery Wei-Ching Liang Scott Stawicki Judy Mak Nisha Rathore Raymond K. Tong Joe Kowalski Sharon Fong Yee Glenn Pacheco Sarajane Ross Zhiyong Cheng Jennifer Le Couter Greg Plowman Franklin Peale Alexander W. Koch Yan Wu Anil Bagri Marc Tess | 2007 | Cancer Cell2007，，1： | 1 |
| 12 | Detection of p16 hypermethylation in circulating plasma DNA of non-small cell lung cancer patients显示文摘 | Qian An Yong Liu Yanning Gao Jinfeng Huang Xiaoli Fong Ling Li Dechao Zhang Shujun Cheng | 2002 | Cancer Letters2002，，1： | 1 |
| 13 | 查看详情显示文摘 | (d) Yan L Huo P Debenham J S Madsen-Duggan C B Lao J Chen R Z Xiao J C Shen C -P Stribling D S Shearman L P Strack A M Tsou N Ball R G Wang J Tong X Bateman T J Reddy V B G Fong T M Hale J J | | 0，，： | 1 |
| 14 | The tribological characteristics of titanium nitride, titanium carborritride and titanium carbide coatings 显示文摘 | Yeong Yan Guu Jen Fin Lin Chi - Fong Ai | 1997 | Thin Solid Films1997，302，： | 1 |
| 15 | Potential of plant as a biological factory to synthesize gold and silver nanoparticles and their applications显示文摘 | Pei Pei Gan Sam Fong Yan Li | 2012 | Rev Environ Sci Biotechnol2012，11，2： | 1 |
| 16 | Natural history and clinical predictors of clinical progression in benign prostatic hyperplasia显示文摘 | Fong Yan Kit Milani | 2005 | Current Opinion in Urology2005，15，1： | 1 |
| 17 | A Logical Model for Detec- ting Irregular Actions in Physical Access Environment 显示文摘 | Antonio Leong Simon Fong Zhuang Yan | 2007 | IEEE Con- ference on Database and Expert Systems Applications2007，，7： | 1 |
| 18 | Application of artifcial intelligence in cataract management:current and future directions显示文摘The rise of artifcial intelligence(AI)has brought breakthroughs in many areas of medicine.In ophthalmology,AI has delivered robust results in the screening and detection of diabetic retinopathy,age-related macular degeneration,glaucoma,and retinopathy of prematurity.Cataract management is another feld that can beneft from greater AI application.Cataract is the leading cause of reversible visual impairment with a rising global clinical burden.Improved diagnosis,monitoring,and surgical management are necessary to address this challenge.In addition,patients in large developing countries often sufer from limited access to tertiary care,a problem further exacerbated by the ongoing COVID-19 pandemic.AI on the other hand,can help transform cataract management by improving automation,efcacy and overcoming geographical barriers.First,AI can be applied as a telediagnostic platform to screen and diagnose patients with cataract using slit-lamp and fundus photographs.This utilizes a deep-learning,convolutional neural network(CNN)to detect and classify referable cataracts appropriately.Second,some of the latest intraocular lens formulas have used AI to enhance prediction accuracy,achieving superior postoperative refractive results compared to traditional formulas.Third,AI can be used to augment cataract surgical skill training by identifying diferent phases of cataract surgery on video and to optimize operating theater workfows by accurately predicting the duration of surgical procedures.Fourth,some AI CNN models are able to efectively predict the progression of posterior capsule opacifcation and eventual need for YAG laser capsulotomy.These advances in AI could transform cataract management and enable delivery of efcient ophthalmic services.The key challenges include ethical management of data,ensuring data security and privacy,demonstrating clinically acceptable performance,improving the generalizability of AI models across heterogeneous populations,and improving the trust of end-users. | Laura Gutierrez Jane Sujuan Lim Li Lian Foo Wei Yan Ng Michelle Yip Gilbert Yong San Lim Melissa Hsing Yi Wong Allan Fong Mohamad Rosman Jodhbir Singth Mehta Haotian Lin Darren Shu Jeng Ting Daniel Shu Wei Ting | 2024 | Eye and Vision2024，10，1： | 0 |
| 19 | Cost-effective hybrid long-short read assembly delineates alternative GC-rich Streptomyces hosts for natural product discovery显示文摘With the advent of rapid automated in silico identification of biosynthetic gene clusters(BGCs),genomics pre-sents vast opportunities to accelerate natural product(NP)discovery.However,prolific NP producers,Strepto-myces,are exceptionally GC-rich(>80%)and highly repetitive within BGCs.These pose challenges in sequencing and high-quality genome assembly which are currently circumvented via intensive sequencing.Here,we outline a more cost-effective workflow using multiplex Illumina and Oxford Nanopore sequencing with hybrid long-short read assembly algorithms to generate high quality genomes.Our protocol involves subjecting long read-derived assemblies to up to 4 rounds of polishing with short reads to yield accurate BGC predictions.We successfully sequenced and assembled 8 GC-rich Streptomyces genomes whose lengths range from 7.1 to 12.1 Mb with a median N50 of 8.2 Mb.Taxonomic analysis revealed previous misrepresentation among these strains and allowed us to propose a potentially new species,Streptomyces sydneybrenneri.Further comprehensive characterization of their biosynthetic,pan-genomic and antibiotic resistance features especially for molecules derived from type I polyketide synthase(PKS)BGCs reflected their potential as alternative NP hosts.Thus,the genome assemblies and insights presented here are envisioned to serve as gateway for the scientific community to expand their avenues in NP discovery. | Elena Heng Lee Ling Tan Dillon W.P.Tay Yee Hwee Lim Lay-Kien Yang Deborah C.S.Seow Chung Yan Leong Veronica Ng Siew Bee Ng Yoganathan Kanagasundaram Fong Tian Wong Lokanand Koduru | 2023 | Synthetic and Systems Biotechnology2023，8，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员