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| 1 | Scalable volumetric imaging for ultrahigh-speed brain mapping at synaptic resolution显示文摘The speed of high-resolution optical imaging has been a rate-limiting factor for meso-scale mapping of brain structures and functional circuits,which is of fundamental importance for neuroscience research.Here,we describe a new microscopy method of Volumetric Imaging with Synchronized on-the-fly-scan and Readout(VISo R)for high-throughput,high-quality brain mapping.Combining synchronized scanning beam illumination and oblique imaging over cleared tissue sections in smooth motion,the VISo R system effectively eliminates motion blur to obtain undistorted images.By continuously imaging moving samples without stopping,the system achieves high-speed 3D image acquisition of an entire mouse brain within1.5 hours,at a resolution capable of visualizing synaptic spines.A pipeline is developed for sample preparation,imaging,3D image reconstruction and quantification.Our approach is compatible with immunofluorescence methods,enabling flexible cell-type specific brain mapping and is readily scalable for large biological samples such as primate brains.Using this system,we examined behaviorally relevant whole-brain neuronal activation in 16 c-Fos-sh EGFP mice under resting or forced swimming conditions.Our results indicate the involvement of multiple subcortical areas in stress response.Intriguingly,neuronal activation in these areas exhibits striking individual variability among different animals,suggesting the necessity of sufficient cohort size for such studies. | Hao Wang Qingyuan Zhu Lufeng Ding Yan Shen Chao-Yu Yang Fang Xu Chang Shu Yujie Guo Zhiwei Xiong Qinghong Shan Fan Jia Peng Su Qian-Ru Yang Bing Li Yuxiao Cheng Xiaobin He Xi Chen Feng Wu Jiang-Ning Zhou Fuqiang Xu Hua Han Pak-Ming Lau Guo-Qiang Bi | 2019 | National Science Review2019，6，5： | 7 |
| 2 | Detection and characterization of diverse alpha-and betacoronaviruses from bats in China显示文摘Bats have been implicated as important reservoir hosts of alpha- and betacoronaviruses. In this study, diverse coronaviruses(CoVs) were detected in 50 of 951(positive rate 5.3%) intestinal specimens of eight bat species collected in four provinces and the Tibet Autonomous Region of China by pan-coronavirus RT-PCR screening. Based on 400-nt RNA-dependent RNA polymerase(Rd RP) sequence analysis, eight belonged to genus Alphacoronavirus and 42 to Betacoronavirus.Among the 50 positive specimens, thirteen gave rise to CoV full-length Rd RP gene amplification for further sequence comparison, of which three divergent sequences(two from a unreported province) were subjected to full genome sequencing. Two complete genomes of betacoronaviruses(JTMC15 and JPDB144) and one nearly-complete genome of alphacoronavirus(JTAC2) were sequenced and their genomic organization predicted. The present study has identified additional numbers of genetically diverse bat-borne coronaviruses with a wide distribution in China. Two new species of bat CoV, identified through sequence comparison and phylogenetic analysis, are proposed. | Lin Xu Fuqiang Zhang Weihong Yang Tinglei Jiang Guanjun Lu Biao He Xingyu Li Tingsong Hu Gang Chen Yun Feng Yuzhen Zhang Quanshui Fan Jiang Feng Hailin Zhang Changchun Tu | 2016 | Virologica Sinica2016，31，1： | 6 |
| 3 | Cortical Organization of Centrifugal Afferents to the Olfactory Bulb: Mono-and Trans-synaptic Tracing with Recombinant Neurotropic Viral Tracers显示文摘Sensory processing is strongly modulated by different brain and behavioral states,and this is based on the top-down modulation.In the olfactory system,local neural circuits in the olfactory bulb(OB)are innervated by centrifugal afferents in order to regulate the processing of olfactory information in the OB under different behavioral states.The purpose of the present study was to explore the organization of neural networks in olfactory-related cortices and modulatory nuclei that give rise to direct and indirect innervations to the glomerular layer(GL)of the OB at the whole-brain scale.Injection of different recombinant attenuated neurotropic viruses into the GL showed that it received direct inputs from each layer in the OB,centrifugal inputs from the ipsilateralanterior olfactory nucleus(AON),anterior piriform cortex(Pir),and horizontal limb of diagonal band of Broca(HDB),and various indirect inputs from bilateral cortical neurons in the AON,Pir,amygdala,entorhinal cortex,hippocampus,HDB,dorsal raphe,median raphe and locus coeruleus.These results provide a circuitry basis that will help further understand the mechanism by which olfactory informationprocessing in the OB is regulated. | Pengjie Wen Xiaoping Rao Liuying Xu Zhijian Zhang Fan Jia Xiaobin He Fuqiang Xu | 2019 | Neuroscience Bulletin2019，35，4： | 4 |
| 4 | Viral Tools for Neural Circuit Tracing显示文摘Neural circuits provide an anatomical basis for functional networks.Therefore,dissecting the structure of neural circuits is essential to understanding how the brain works.Recombinant neurotropic viruses are important tools for neural circuit tracing with many advantages over non-viral tracers:they allow for anterograde,retrograde,and trans-synaptic delivery of tracers in a cell type-specific,circuit-selective manner.In this review,we summarize the recent developments in the viral tools for neural circuit tracing,discuss the key principles of using viral tools in neuroscience research,and highlight innovations for developing and optimizing viral tools for neural circuit tracing across diverse animal species,including nonhuman primates. | Qing Liu Yang Wu Huadong Wang Fan Jia Fuqiang Xu | 2022 | Neuroscience Bulletin2022，38，12： | 4 |
| 5 | Rapid and Sparse Labeling of Neurons Based on the Mutant Virus-Like Particle of Semliki Forest Virus显示文摘Sparse labeling of neurons contributes to uncovering their morphology, and rapid expression of a fluorescent protein reduces the experiment range. To achieve the goal of rapid and sparse labeling of neurons in vivo, we established a rapid method for depicting the fine structure of neurons at 24 h post-infection based on a mutant viruslike particle of Semliki Forest virus. Approximately 0.014 fluorescent focus-forming units of the mutant virus-like particle transferred enhanced green fluorescent protein into neurons in vivo, and its affinity for neurons in vivo was stronger than for neurons in vitro and BHK21(baby hamster kidney) cells. Collectively, the mutant virus-likeparticle provides a robust and convenient way to reveal the fine structure of neurons and is expected to be a helper virus for combining with other tools to determine their connectivity. Our work adds a new tool to the approaches for rapid and sparse labeling of neurons in vivo. | Fan Jia Xutao Zhu Pei Lv Liang Hu Qing Liu Sen Jin Fuqiang Xu | 2019 | Neuroscience Bulletin2019，35，3： | 3 |
| 6 | Experimental Studies on Fuel Spray Characteristics of Pressure-Swirl Atomizer and Air-Blast Atomizer显示文摘In this work,the effects of fuel temperatures and pressure drops on the flow field and spray characteristics of a pressure-swirl atomizer were discussed using the Particle Imaging Velocimetry(PIV),Planar Laser Induced Fluorescence(PLIF)and Laser Particle Size Analyzer(LPSA)methods.Then the air-blast atomizer was selected to study the interaction of initial atomization and flow field.The effect of fuel-air ratio on the air-blast atomizer were also considered,where the fuel-air ratio was varied by adjusting mass flow rate of the air and fuel respectively.The results show that the spray angle of the pressure-swirl atomizer increases first and changes a little after the pressure drop higher than 0.5 MPa.However,more fuel concentrate on the central region,which is mainly caused by the increase of the proportion of small droplets with lower centrifugal force.The fuel temperature can improve the spray angle only in lower pressure drop,and it has a little effect under higher pressure drops.In addition,the fuel pressure drop has an obvious influence on the fuel distribution and flow field near the nozzle exit compared with the downstream.For the air-blast atomizer,the spray angle increases compared with the pressure-swirl atomizer for the introduction of swirl air.Furthermore,the spray angle decreases with the air mass rate increasing,and it increases with the fuel mass rate increasing.The distribution of velocity and droplet near the nozzle exit is influenced by the air mass rate,and the fuel mass rate mainly affects the distribution in the downstream.The fuel accumulates in the annular area below the nozzle,and the distribution of it changes little with the development along the axial direction. | WANG Kaixing FAN Xiongjie LIU Fuqiang LIU Cunxi LU Haitao XU Gang | 2021 | Journal of Thermal Science2021，30，2： | 1 |
| 7 | Spatiotemporal variations of carbon flux and nitrogen deposition flux linked with climate change at the centennial scale in China显示文摘The spatial and temporal variations of the vegetation carbon flux in China from 1901 to 2005 were studied using the vegetation net primary production(NPP)values from seven Earth-system models.In addition,the temporal and spatial changes in the nitrogen deposition flux in China were studied using the NHx and NOy fluxes from 1901 to 2005.The relationship between changes in the carbon flux,nitrogen flux and climate was analyzed.The results show that(1)over the past 100 years,NPP in China has shown an upward trend.The average trend coefficient is 0.88 and the NPP distribution trend is generally low in the north and high in the south,with a gradual increase from the northwest to the southeast.Temperature,precipitation and radiation are all conducive to plant growth in the direction of the gradient.The correlation coefficients between the ensemble model mean NPP and temperature,precipitation,longwave radiation and shortwave radiation are 0.88,0.73,0.91 and 0.67,respectively.(2)In the past 100 years,the NHx and NOy fluxes in China have shown an upward trend,with trend coefficients of 0.98 and 0.98,respectively,which pass the 99.9%confidence level of the t-test.NHx and NOy fluxes are also generally low in the north and high in the south,with a gradual increase from the northwest to the southeast in a step-like pattern.(3)The spatial distribution of the correlation coefficients between the NHx and NOy fluxes and air temperature is similar,with only slight differences in values.The spatial distribution of the correlation coefficients between the NHx and NOy fluxes and precipitation is similar in overall pattern,but the pattern is relatively complicated,with a positive-negative-positive-negative-positive pattern occurring across the monsoon region from north to south,and a negative-positive-negative-positive pattern occurring beyond the monsoon region from east to west.(4)The spatial distribution of the correlation coefficients between the NHx and NOy fluxes and NPP shows a generally consistent pattern,but the pattern is relatively uneven.The average distribution of the ensemble model mean is positive correlation in northeast China and southwest China,and alternating positive and negative correlation in other regions. | Dongdong GAO Li DAN Guangzhou FAN Hanqin TIAN Jing PENG Xiujing YANG Fuqiang YANG Yueyue LI | 2020 | Science China Earth Sciences2020，63，5： | 1 |
| 8 | The Effect of Different Reaction Mechanisms on Combustion Simulation of a Reverse-Flow Combustor显示文摘Three different reaction mechanisms of kerosene and flamelet models were used to simulate combustion in a reverse-flow combustor.By comparing the effects of different mechanisms on the flow field characteristics,components and temperature distribution of the combustion chamber,the results showed that:Under different reaction mechanisms,there was a strong similarity between flow filed and temperature field,but the penetration depth and temperature distribution of local jets were affected by the mechanism.Due to the different reaction paths and reaction rates,the distribution of major components had a great degree of similarity,but the concentration of intermediate components varied greatly.Comprehensive analysis,the 16 species and 17 species reaction mechanisms can simulate the flow field and outlet temperature distribution of the combustor well. | LU Haitao LIU Fuqiang WANG Yulan FAN Xiongjie LIU Cunxi XU Gang | 2020 | Journal of Thermal Science2020，29，3： | 1 |
| 9 | Detection of hepatitis B surface antigen, hepatitis B core antigen, and hepatitis B virus DNA in parotid tissues显示文摘 | Lizhang Chen Fuqiang Liu Xuegong Fan Jianming Gao Nengzhi Chen Tom Wong Jun Wu Shi Wu Wen | 2008 | International Journal of Infectious Diseases2008，，1： | 1 |
| 10 | The 2009 pandemic A/Wenshan/01/2009 H1N1 induces apoptotic cell death in human airway epithelial cells显示文摘In 2009,a novel swine-origin H1N1 influenza virus emerged in Mexico and quickly spread to other countries,including China.This 2009 pandemic H1N1 can cause human respiratory disease,but its pathogenesis remains poorly understood.Here,we studied the infection and pathogenesis of a new 2009 pandemic strain,A/Wenshan/01/2009 H1N1,in China in human airway epithelial cell lines compared with contemporary seasonal H1N1 influenza virus.Our results showed that viral infection by the A/Wenshan H1N1 induced significant apoptotic cell death in both the human nasopharyngeal carcinoma cell line CNE-2Z and the human lung adenocarcinoma cell line A549.The A/Wenshan H1N1 virus enters both of these cell types more efficiently than the seasonal influenza virus.Viral entry in both cell lines was shown to be mediated by clathrin-and dynamin-dependent endocytosis.Therefore,we discovered that the 2009 pandemic H1N1 strain,A/Wenshan/01/2009,can induce apoptotic cell death in epithelial cells of the human respiratory tract,suggesting a molecular pathogenesis for the 2009 pandemic H1N1. | Ning Yang Xiaoxu Hong Penghui Yang Xiangwu Ju Yuguo Wang Jun Tang Chenggang Li Quanshui Fan Fuqiang Zhang Zhongwei Chen Li Xing Zhongpeng Zhao Xiao Gao Guoyang Liao Qihan Li Xiliang Wang Dangsheng Li Chengyu Jiang | 2011 | Journal of Molecular Cell Biology2011，3，4： | 0 |
| 11 | Occurrence and Roles of Comammox Bacteria in Water and Wastewater Treatment Systems:A Critical Review显示文摘Nitrogen removal is a critical process in water treatment plants(WIPs)and wastewater treatment plants(WWTPs).The recent discovery of a novel bacterial process,complete ammonia oxidation(comammox,CMX),has refuted a century-long perception of the two-step conversion of NH3to NO3-.Compared with canonical nitrifiers,CMX bacteria offer undeniable advantages,such as a high growth yield propensity and adaptability to nutrient-and growth-limiting conditions,which collectively draw attention to validate the aptness of CMX bacteria to wastewater treatment.As there has been no comprehensive review on the relevance of CMX bacteria for sustainable water and wastewater treatment,this review is intended to discuss the roles and applications of CMX in the removal of nitrogen and pollutants from water and wastewater.We took into account insights into the metabolic versatilities of CMX bacteria at the clade and subclade levels.We focused on the distribution of CMX bacteria in engineered systems,niche differentiation,co-occurrence and interactions with cano nical nitrifiers for a better understanding of CMX bacteria in terms of their ecophysiology.Conceptualized details on the reactor adaptability and stress response of CMX bacteria are provided.The potential of CMX bacteria to degrade micropollutants either directly or co-metabolically was evaluated,and these insights would be an indispensable advantage in opening the doors for wider applications of CMX bacteria in WWTPs.Finally,we summarized future directions of research that are imperative in improving the understanding of CMX biology. | Naga Raju Maddela Zhihao Gan Yabing Meng Fuqiang Fan Fangang Meng | 2022 | Engineering2022，，10： | 0 |
| 12 | 本校近期发表IF≥4.0的SCI论文摘要(英文)--Evaluation of Blastomere Biopsy Using a Mouse Model Indicates the Potential High Risk of Neurodegenerative Disorders in the Offspring显示文摘 | Wu Jindao Guo Xuejiang Zhao Chun Zhou Rong Zhang Zhuo Wang Fuqiang Xiao Min Chen Ling Zhu Hui Chen Wen Lin Min Liu Jiayin Zhou Zuomin Wang Liu Huo Ran Sha Jiahao Yu Yang Fan Yong Lv Zhuo Wang Liu Zhou Qi | 2009 | 南京医科大学学报(自然科学版)2009，29，9： | 0 |
| 13 | The eff ect of Rhizophagus irregularis on salt stress tolerance of Elaeagnus angustifolia roots显示文摘We assessed the eff ects of arbuscular mycorrhizal fungi(AMF)Rhizophagus irregularis inoculation on salt stress tolerance in roots of the drought-tolerant plant Elaeagnus angustifolia.We studied a plant growth index,spore density and hyphal length density of AMF,the Na+contents and ultrastructure of root cells,as well as rhizosphere soil enzyme activities of mycorrhizal and non-mycorrhizal E.angustifolia seedlings under diff erent salt stress.Under salt stress,growth of E.angustifolia with mycorrhizal inoculation was higher than that of non-inoculated treatments.The spore density and hyphal length density decreased signifi cantly under salt stress in rhizosphere soil of mycorrhizal E.angustifolia seedlings(p<0.05).The root cells of E.angustifolia seedlings inoculated with R.irregularis at 300 mmol L−1 salt had more organelles,greater integrity,and lower root Na+contents than those of non-inoculated seedlings.In addition,the results showed notably higher activities of catalase,phosphatase,urease and saccharase in rhizosphere soil of the mycorrhizal seedlings in response to salinity compared to those of the non-mycorrhizal seedlings.Therefore,AMF inoculation could enhance salt stress tolerance in roots of E.angustifolia. | Wenyuan He Xiaoxu Fan Zixin Zhou Huanhuan Zhang Xiang Gao Fuqiang Song Gui Geng | 2020 | Journal of Forestry Research2020，31，6： | 0 |
| 14 | Mutation inε-Sarcoglycan Induces a Myoclonus-Dystonia Syndrome-Like Movement Disorder in Mice显示文摘Myoclonus dystonia syndrome(MDS)is an inherited movement disorder,and most MDS-related mutations have so far been found in theε-sarcoglycan(SGCE)coding gene.By generating SGCE-knockout(KO)and human 237 C>T mutation knock-in(KI)mice,we showed here that both KO and KI mice exerted typical movement defects similar to those of MDS patients.SGCE promoted filopodia development in vitro and inhibited excitatory synapse formation both in vivo and in vitro.Loss of function of SGCE leading to excessive excitatory synapses that may ultimately contribute to MDS pathology.Indeed,using a zebrafish MDS model,we found that among 1700 screened chemical compounds,Vigabatrin was the most potent in readily reversing MDS symptoms of mouse disease models.Our study strengthens the notion that mutations of SGCE lead to MDS and most likely,SGCE functions to brake synaptogenesis in the CNS. | Jiao Li Yiqiong Liu Qin Li Xiaolin Huang Dingxi Zhou Hanjian Xu Feng Zhao Xiaoxiao Mi Ruoxu Wang Fan Jia Fuqiang Xu Jing Yang Dong Liu Xuliang Deng Yan Zhang | 2021 | Neuroscience Bulletin2021，37，3： | 0 |
| 15 | Optimization of spectral distortion in a ytterbium-doped mode-locked fiber laser system显示文摘A method for optimizing the spectral distortion of an ultrafast pulse in a polarization-maintaining picosecond linear-cavity fiber laser with a one-stage fiber amplifier is proposed and demonstrated. The mechanism of control of the spectral distortion in the fiber system has been investigated. The experimental and theoretical results illustrate that the filtering effect of a fiber Bragg grating can effectively decrease the spectral oscillatory distortion accumulated by self-phase modulation. Injected into a Nd:YAG regenerative amplifier, the ultrafast pulse could produce high pulse energy of 1.2 mJ at a repetition rate of 1 kHz. | Fuqiang Lian Zhongwei Fan Zhenao Bai Xiaohui Li Qi Jie Wang | 2015 | Photonics Research2015，3，4： | 0 |
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