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| 1 | Serum bile acid profiling reflects enterohepatic detoxification state and intestinal barrier function in inlammatory bowel disease显示文摘AIM:To determine free and conjugated serum bile acid (BA) levels in in? ammatory bowel disease (IBD) subgroups with defi ned clinical manifestations.METHODS: Comprehensive serum BA profiling was performed in 358 IBD patients and 310 healthy controls by liquid chromatography coupled to electrospray ionization tandem mass spectrometry.RESULTS: Serum levels of hyodeoxycholic acid, the CYP3A4-mediated detoxification product of the secondary BA lithocholic acid (LCA), was increased significantly in Crohn's disease (CD) and ulcerative colitis (UC), while most other serum BA species were decreased significantly. Total BA, total BA conjugate, and total BA glycoconjugate levels were decreased only in CD, whereas total unconjugated BA levels were decreased only in UC. In UC patients with hepatobiliary manifestations, the conjugated primary BAs glycocholic acid, taurocholic acid, and glycochenodeoxycholic acid were as significantly increased as the secondary BAs LCA, ursodeoxycholic acid, and tauroursodeoxycholic acid compared to UC patients without hepatobiliary manifestations. Finally, we found that in ileocecal resected CD patients, the unconjugated primary BAs, cholic acid and chenode-oxycholic acid, were increased significantly compared to controls and patients without surgical interventions.CONCLUSION: Serum BA profiling in IBD patients that indicates impaired intestinal barrier function and increased detoxification is suitable for advanced diagnostic characterization and differentiation of IBD subgroups with defined clinical manifestations. | Carsten Gnewuch Gerhard Liebisch Thomas Langmann Benjamin Dieplinger Thomas Mueller Meinhard Haltmayer Hans Dieplinger Alexandra Zahn Wolfgang Stremmel Gerhard Rogler Gerd Schmitz | 2009 | World Journal of Gastroenterology2009，15，25： | 4 |
| 2 | Lithocholic acid induction of the FGF19 promoter in intestinal cells is mediated by PXR显示文摘AIM: To study the effect of the toxic secondary bile acid lithocholic acid (LCA) on the expression of fibroblast growth factor 19 (FGF19) in intestinal cells and to characterize the pregnane-X-receptor (PXR) response of the FGF19 promoter region. METHODS: The intestinal cell line LS174T was stimulated with various concentrations of chenodeoxy-cholic acid and lithocholic acid for several time points. FGF19 mRNA levels were determined with quantitative realtime RT-PCR. FGF19 deletion promoter constructs were generated and the LCA response was analzyed in reporter assays. Co-transfections with PXR and RXR were carried out to study FGF19 regulation by these factors. RESULTS: LCA and CDCA strongly up-regulate FGF19 mRNA expression in LS174T cells in a time and dose dependent manner. Using reporter gene assays with several deletion constructs we found that the LCA responsive element in the human FGF19 promoter maps to the proximal regulatory region containing two poten-tial binding sites for PXR. Overexpression of PXR and its dimerization partner retinoid X receptor (RXR) and stimulation with LCA or the potent PXR ligand rifampicin leads to a signifi cant induction of FGF19 promoter activ-ity in intestinal cells. CONCLUSION: LCA induced feedback inhibition of bile acid synthesis in the liver is likely to be regulated by PXR inducing intestinal FGF19 expression. | Wolfgang Wistuba Carsten Gnewuch Gerhard Liebisch Gerd Schmitz Thomas Langmann | 2007 | World Journal of Gastroenterology2007，13，31： | 4 |
| 3 | Rapid quantifi- cation of bile acids and their conjugates in serum by liquid chro- matography - tandem mass spectrometry 显示文摘 | Scherer M Carsten Gnewuch C Schmitz G | 2009 | Journal of Chroma- tography B2009，877，30： | 1 |
| 4 | Changes in HDL-associated apolipoproteins relate to mortality in human sepsis and correlate to monocyte and platelet activation显示文摘 | Barlage S Gnewuch C Liebisch G | 2009 | Intensive Care Med2009，35，11： | 1 |
| 5 | Optical Anisotropy of Dispersed Carbon Nanotubes Induced by an Electric Field显示文摘 | Bubke K Gnewuch H Hempstead M | 1997 | Appl Phys Lett1997，71，14： | 1 |
| 6 | Optical anisotropy ofdispersed carbon nanotubes induced by an electric field显示文摘 | Bubke K Gnewuch H Hempstead M | 1997 | Applied Physics Letters1997，71，： | 1 |
| 7 | Broadband monilithic acousto-optic tunable filter显示文摘 | GNEWUCH H ZAYER N K PANNELL C N | | 0，，05： | 1 |
| 8 | Optical anisotropy of dispersed carbon nanotubes induced by an electric filed 显示文摘 | Bubke K Gnewuch H Henpstead M | 1997 | Appl Phys Lett1997，71，14： | 1 |
| 9 | Optical anisotropy of dispersed carbon nanotubes induced by an electric field显示文摘 | Bubke K Gnewuch H Hempstead M | | 0，，： | 1 |
| 10 | Changes in HDL-associated apolipoproteins relate to mortality in human sepsis and correlate to monocyte and platelet activation显示文摘 | Barlage S Gnewuch C Liebisch G | 2009 | Intensive Care Med2009，35，11： | 1 |
| 11 | Clearance of vancomycin during continuous infusion in Intensive Care Unit patients: correlation with measured and estimated creatinine clearance and serum cystatin C显示文摘 | Kees MG Hilpert JW Gnewuch C | 2010 | Int J Antimicrob Agents2010，36，： | 1 |
| 12 | Optical Anisotropy of Dispersed Carbon Nanotubes Induced by an Electric-field显示文摘 | Bubkle K Gnewuch H Hempstead M | 1997 | Applied Physics Letter1997，，71： | 1 |
| 13 | Optical anisotropy of dispersed carbonnanotubes induced by an electric-field显示文摘 | Bubkle K Gnewuch H Hempstead M | 1997 | App l Phys Lett1997，71，14： | 1 |
| 14 | Electro-Optic Electric Field Sensors Base on Periodically Poled NiNbO3显示文摘 | Rao Y J Gnewuch H Pannell C N | 1999 | Electronics Letters1999，36，7： | 1 |
| 15 | Clearance of vancomycin during continuous infusion in intensive care unit patients: correlation with measured and estimated creatinine clearance and serum cystatin C 显示文摘 | KEES M G HILPERT J W GNEWUCH C | 2010 | Int J Antimicrob Agents2010，36，6： | 1 |
| 16 | Changes in HDL-asseciated apolipopreteins relate to mortality in human sepsis and correlate to monocyte and platelet activation显示文摘 | Barlage S Gnewuch C Liebisch G | 2009 | Intensive Care Med2009，35，11： | 1 |
| 17 | Quasi-polynomial tractability 显示文摘 | Gnewuch M Woziakowski H | 2011 | Journal of Complexity2011，27，34： | 1 |
| 18 | A Critical Appraisal of the E- volution of N-Nitrosoureas as Anticancer Drugs显示文摘 | Gnewuch CT Sosnovsky G | 1997 | Chem Rev1997，97，3： | 1 |
| 19 | Rapid quantifica- tion of bile acids and their conjugates in serum by liquid chromatography-tandem mass spectrometry显示文摘 | Scherer M Gnewuch C Schmitz G | 2009 | J Chromatogr B Analyt Technol Biomed Life Sci2009，877，30： | 1 |
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