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| 1 | Rapid generation of genetic diversity by multiplex CRISPR/Cas9 genome editing in rice显示文摘The clustered regularly interspaced short palindromic repeats(CRISPR)-associated endonuclease 9(CRISPR/Cas9) system has emerged as a promising technology for specific genome editing in many species. Here we constructed one vector targeting eight agronomic genes in rice using the CRISPR/Cas9 multiplex genome editing system. By subsequent genetic transformation and DNA sequencing, we found that the eight target genes have high mutation efficiencies in the T_0 generation. Both heterozygous and homozygous mutations of all editing genes were obtained in T_0 plants. In addition, homozygous sextuple, septuple, and octuple mutants were identified. As the abundant genotypes in T_0 transgenic plants, various phenotypes related to the editing genes were observed. The findings demonstrate the potential of the CRISPR/Cas9 system for rapid introduction of genetic diversity during crop breeding. | Lan Shen Yufeng Hua Yaping Fu Jian Li Qing Liu Xiaozhen Jiao Gaowei Xin Junjie Wang Xingchun Wang Changjie Yan Kejian Wang | 2017 | Science China(Life Sciences)2017，60，5： | 11 |
| 2 | Characteristics of authigenic pyrite and its sulfur isotopes influenced by methane seep at Core A, Site 79 of the middle Okinawa Trough显示文摘The anaerobic oxidation of methane(AOM) has strongly developed at Core A, Site 79 of the middle Okinawa Trough, East China Sea, and a large amount of authigenic pyrite is preserved in the surface sediment. In this study, we analyze the characteristics of the authigenic pyrite and its sulfur isotopic values. The authigenic pyrite is stripy and tubular, and there were foraminifera compartments filled with pyrite. The pyrite is extracted using chromium reduction, and the values of ? 34 S are found to lie between ?41.20‰ and 8.92‰ V-CDT. The bulk pyrite tends to be more enriched in 34 S with increasing depth. Particularly, the ? 34 S value of the pyrite lies between ?32.73‰ and ?41.20‰ V-CDT above 278 cmbsf, but it quickly increases below this depth(?21.49‰–8.92‰ V-CDT). At the same time, the total sulfur content of the pyrite shows an abrupt increase above 100 cmbsf but is otherwise stable between 1.04% and 0.55% below 100 cmbsf. The stable and negative values of ? 34 S and the decreasing values of total sulfur above 278 cmbsf indicate reduced AOM activities in 17.18–5.3 ka. In addition, the increasing ? 34 S and pyrite content indicate strong AOM development and methane seep below 278 cmbsf in 18.8–17.18 ka. In particular, the highest positive value of ? 34 S occurring in 18.78 ka indicates the most intense AOM activity. The shallow sulfate-methane interface(SMI) and high methane flux below marine sediments also strongly support this activity. | WANG Meng CAI Feng LI Qing LIANG Jie YAN GuiJing DONG Gang WANG Feng SHAO HeBin HU GaoWei | 2015 | Science China Earth Sciences2015，58，12： | 4 |
| 3 | An aqueous zinc-ion hybrid super-capacitor for achieving ultrahigh-volumetric energy density显示文摘Zinc-ion hybrid super-capacitors are regarded as promising safe energy storage systems,However,the relatively low volumetric energy density has become the main bottlenecks in practical applications of portable electronic devices,In this work,the zinc-ion hybrid super-capacitor with high volumetric energy density and superb cycle stability had been constructed which employing the high-density threedimensional graphene hydrogel as cathode and Zn foil used as anode in 1 mol/L ZnSO4 electrolyte.Benefiting from the abundant ion transport paths and the abundant active sites for graphene hydrogel with high density and porous structure,the zinc-ion hybrid super-capacitor exhibited an extremely high volumetric energy density of 118.42 Wh/L and a superb power density of 24.00 kW/L,as well as an excellent long cycle life(80% retention after 30,000 cycles at 10 A/g),which was superior to the volumetric energy density of the reported zinc-ion hybrid super-capacitors.This device,based on the fast ion adsorption/deso rption on the capacitor-type graphene cathode and reversible Zn^(2+) plating/stripping on the battery-type Zn anode,which will inspire the development of zinc-ion hybrid super-capacitor in miniaturized devices. | Li Zhang Dandan Wu Gaowei Wang Yongtai Xu Hongxia Li Xingbin Yan | 2021 | Chinese Chemical Letters2021，32，2： | 4 |
| 4 | Integrative Multi-Spectral Sensor Device for Far-Infrared and Visible Light Fusion显示文摘红外线、可见的轻图象熔化技术是在最近的年里的多传感器熔化技术的研究的一个热点。存在因为使用二个照相机,红外线、可见的轻熔化技术需要登记在熔化前。然而,登记技术的申请效果还得被改进。因此,一台新奇综合多光谱的传感器设备为红外线、可见的轻熔化被建议,并且由使用横梁 splitter 棱柱,从一样的透镜的同轴的轻事件被投射到红外线的费用联合设备(电荷耦合器件) 和可见轻电荷耦合器件分别地。在这篇论文,建议传感器设备的成像机制与信号获得和熔化的过程被学习。模拟实验,包含眼的系统,信号获得,和信号熔化的全部进程,基于成像效果模型被构造。另外,优秀评估索引被采用分析模拟结果。试验性的结果证明建议传感器设备有效、可行。 | Tiezhu QIAO Lulu CHEN Yusong PANG Gaowei YAN | 2018 | Photonic Sensors2018，8，2： | 3 |
| 5 | Erratum to: Rapid generation of genetic diversity by multiplex CRISPR/Cas9 genome editing in rice显示文摘The same figure was misused for the PCR/RE assay results of Gn1a and GW2 fragments in Figure 3,and the arrows in the graphicsal result of GW2 were not on the tape.The corrected Figure 3 is as follows. | Lan Shen Yufeng Hua Yaping Fu Jian Li Qing Liu Xiaozhen Jiao Gaowei Xin Junjie Wang Xingchun Wang Changjie Yan Kejian Wang | 2019 | Science China(Life Sciences)2019，62，9： | 1 |
| 6 | An Effective Refinement Artificial Bee Colony Optimization Algorithm Based on Chaotic Search and Application for PID Control Tuning显示文摘 | Yan Gaowei Li Chuangqin | 2011 | Journal of Computational Information Systems2011，7，9： | 1 |
| 7 | Application of BP Network-Based Multi-sensor Fusion Techniques in measurement of the Unburned Carbon in Fly Ash显示文摘 | Gaowei Yan Gang Xie Keming Xie | 2005 | ISNN LNCS2005，34，： | 1 |
| 8 | MEA Based Nonlinearity Correction Algorithm for the VCO of LFMCW Radar Level Gauge显示文摘 | YAN Gaowei XIE Gang QIU Yuxla and CHEN Zehua | 2005 | Springer Berlin / Heidelberg2005，，： | 1 |
| 9 | Application of BP Network-Based Multi-sensor Fusion Techniques in measurement of the Unburned Carbon in Fly Ash显示文摘 | Gaowei Yan Gang Xie Keming Xie | 2005 | ISNN2005，3498，： | 1 |
| 10 | Multipath Suppressing Method Based on Pseudorange Model Using Modified Teaching-Learning Based Optimization Algorithm显示文摘Satellites based positioning has been widely applied to many areas in our daily lives and thus become indispensable,which also leads to increasing demand for high-positioning accuracy.In some complex environments(such as dense urban,valley),multipath interference is one of the main error sources deteriorating positioning accuracy,and it is difficult to eliminate via differential techniques due to its uncertainty of occurrence and irrelevance in different instants.To address this problem,we propose a positioning method for global navigation satellite systems(GNSS)by adopting a modified teachinglearning based optimization(TLBO)algorithm after the positioning problem is formulated as an optimization problem.Experiments are conducted by using actual satellite data.The results show that the proposed positioning algorithm outperforms other algorithms,such as particle swarm optimization based positioning algorithm,differential evolution based positioning algorithm,variable projection method,and TLBO algorithm,in terms of accuracy and stability. | CHENG Lan ZHANG Jing NI Zihang YAN Gaowei | 2022 | Chinese Journal of Electronics2022，31，5： | 0 |
| 11 | Oscillation quenching and physical explanation on freeplay-based aeroelastic airfoil in transonic viscous flow显示文摘Limit Cycle Oscillation(LCO)quenching of a supercritical airfoil(NLR 7301)considering freeplay is investigated in transonic viscous flow.Computational Fluid Dynamics(CFD)based on Navier-Stokes equations is implemented to calculate transonic aerodynamic forces.A loosely coupled scheme with steady CFD and an efficient graphic method are developed to obtain the aerodynamic preload.LCO quenching phenomenon is observed from the nonlinear dynamic aeroelastic response obtained by using time marching approach.As the airspeed increases,LCO appears then quenches,forming the first LCO branch.Following the quenching region,LCO occurs again and sustains until the divergence of the response,forming the second LCO branch.The quenching of LCOs was addressed physically based on the aerodynamic preload and the linear flutter characteristic.An“island”of stable region is observed in the flutter boundary,i.e.the flutter speed versus the mean Angle of Attack(AoA).The LCO quenches when the aerodynamic preload crosses this stable region with the increasing of airspeed.The LCO quenching of this model in transonic flow is essentially induced by destabilizing effect from aerodynamic preload,since the flutter speed is sensitive to AoA due to aerodynamic nonlinearity. | Yayun SHI Shun HE Gaowei CUI Gang CHEN Yan LIU | 2023 | Chinese Journal of Aeronautics2023，36，10： | 0 |
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