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| 1 | Genetically modified CD7-targeting allogeneic CAR-T cell therapy with enhanced efficacy for relapsed/refractory CD7-positive hematological malignancies:a phase I clinical study显示文摘Chimeric antigen receptor(CAR)-T cell therapy against T cell malignancies faces major challenges including fratricide between CAR-T cells and product contamination from the blasts.Allogeneic CAR-T cells,generated from healthy donor T cells,can provide ready-to-use,blast-free therapeutic products,but their application could be complicated by graft-versus-host disease(GvHD)and host rejection.Here we developed healthy donor-derived,CD7-targeting CAR-T cells(RD13-01)with genetic modifications to resist fratricide,GvHD and allogeneic rejection,as well as to potentiate antitumor function.A phase I clinical trial(NCT04538599)was conducted with twelve patients recruited(eleven with T cell leukemia/lymphoma,and one with CD7-expressing acute myeloid leukemia).All patients achieved pre-set end points and eleven proceeded to efficacy evaluation.No dose-limiting toxicity,GvHD,immune effector cell-associated neurotoxicity or severe cytokine release syndrome(grade≥3)were observed.28 days post infusion,81.8%of patients(9/11)showed objective responses and the complete response rate was 63.6%(7/11,including the patient with AML).3 of the responding patients were bridged to allogeneic hematopoietic stem cell transplantation.With a median follow-up of 10.5 months,4 patients remained in complete remission.Cytomegalovirus(CMV)and/or Epstein-Barr virus(EBV)reactivation was observed in several patients,and one died from EBV-associated diffuse large B-cell lymphoma(DLBCL).Expansion of CD7-negative normal T cells was detected post infusion.In summary,we present the first report of a Phase I clinical trial using healthy donor-derived CD7-targeting allogeneic CAR-T cells to treat CD7^(+)hematological malignancies.Our results demonstrated the encouraging safety and efficacy profiles of the RD13-01 allogeneic CAR-T cells for CD7^(+)tumors. | Yongxian Hu Yali Zhou Mingming Zhang Houli Zhao Guoqing Wei Wengang Ge Qu Cui Qitian Mu Gong Chen Lu Han Tingting Guo Jiazhen Cui Xiaoyan Jiang Xiujun Zheng Shuhui Yu Xiaolong Li Xingwang Zhang Mingxi Chen Xiuju Li Ming Gao Kang Wang Cheng Zu Hao Zhang Xiaohong He Yanbin Wang Dongrui Wang Jiangtao Ren He Huang | 2022 | Cell Research2022，32，11： | 2 |
| 2 | Effect of electroacupuncture on E-prostanoid 2 expression in the spinal cord in rats with neuropathic pain显示文摘OBJECTIVE: To observe the effect of electroacupuncture(EA) treatment on the expression of E-prostanoid 2(EP2) in the spinal cord using a rat model of neuropathic pain, and to examine the effect of different acupuncture prescriptions.METHODS: Rats were randomly divided into six groups: a normal group, model group, sham group,EA 1 group(distal-proximal acupoints), EA 2 group(proximal acupoints), and EA 3 group(distal acupoints). Rats in the EA groups were treated for20 min, once daily for 7 days. The mechanical withdrawal threshold was evaluated at 1 day preoperatively and at 3, 5, and 7 days postoperatively. At 7days postoperatively, hematoxylin-eosin(HE) staining was used to investigate spinal morphological changes. Spinal EP2 messenger ribonucleic acid(m RNA) was detected using reverse-transcription polymerase chain reaction.RESULTS: After the EA treatment, the mechanical withdrawal threshold was up-regulated compared with the model group(P < 0.05). The expression of EP2 m RNA was up-regulated in the spinal cord on day 7 after surgery(P < 0.05). EA stimulation tended to down-regulate EP2 m RNA expression compared with the model group(P > 0.05). The EA 1and EA 2 groups showed a larger treatment effect than the EA 3 group. The HE results showed that EA can improve spinal histomorphology induced by neuropathic pain.CONCLUSION: EA had a strong analgesic and anti-inflammatory effect. The therapeutic effect of EA on the down-regulation of spinal EP2 m RNA expression may be greater when either local acupoints or a distal-proximal combination of acupoints are used than when distal points are used. | Ji Lili Jiang Huili Ren Xiujun Guo Mengwei Ge Dongyu Li Genmao Tu Ya | 2017 | Journal of Traditional Chinese Medicine2017，37，4： | 1 |
| 3 | Effects of Exposure to Lead and Cadmium on the Oxidative Damage of Livers in Laying Hens显示文摘[Objective] To detect the effects of exposure to lead and cadmium on the oxidative damage of livers in laying hens. [Methods] One hundred and twenty 40-week-old Hyline brown hens were randomly divided into four groups. 100 mg / L Pb and / or 50 mg / L Cd was added into the drinking water for eight weeks. [Results] Compared with control group,AST and ALT activities in Pb group enhanced; but there were no significant differences. AST and ALT activities in Cd group and( Pb + Cd) group significantly or extremely significantly increased( P < 0. 05 or P < 0. 01). SOD activity,GSH- Px activity and GSH content in( Pb + Cd) group,Cd group and Pb group were significantly or extremely significantly lower than those in control group( P <0. 05 or P <0. 01). Among them,( Pb + Cd) group showed the greatest reduction( P <0. 01). MDA contents in the three groups were significantly higher than that of control group; and( Pb +Cd) group was significantly higher than Pb group and Cd group. Cu,Fe and Zn contents in three groups were higher than those in control group in different degrees( P <0. 05 or P <0. 01). Se contents in Cd group and( Pb + Cd) group were significantly lower than that in control group( P <0. 01). Residue contents in livers in Pb group and Cd group were significantly greater than that in control group; while residue content in( Pb + Cd) group was significantly higher than those in Pb group and Cd group. Ultrastructure showed that there were symptoms of mitochondrial swelling and fractured cristae in liver cells of laying hens after the exposure to Cd and Pb. In( Pb + Cd) group,these symptoms were even greater. [Conclusion] Oxidative damage and disturbance of trace element metabolism were one of the mechanisms for hepatotocity in laying hens induced by Pb and Cd,and synergistic effect lied in the coadministration. | Chen Dawei Pu Junhua Tang Xiujun Lu Junxian Liu Yinyin Jia Xiaoxu Ge Qinglian Gao Yushi | 2014 | Animal Husbandry and Feed Science2014，6，5： | 1 |
| 4 | Measuremnet of diffusion coefficient of heavy oil in fluidizcd catalylic cracking (FCC) catalysts显示文摘 | Liu Ziyuan Chen Shengli Ge Xiujun | 2010 | Energy & Fuels2010，24，5： | 1 |
| 5 | Identification of Avian-Derived Ingredients in Livestock and Poultry Meat by PCR Technology显示文摘In order to establish a quick and specific method which could identify the avian-derived ingredients,this study used 16 S rRNA gene sequence as target site,and designed the specific primers of chicken,pigeon meat and quail meat. The DNA of common livestock and poultry meat( including mutton,beef,pork,rabbit meat,pigeon meat,quail meat,chicken,duck and goose) was used as template. Though PCR amplification and specific detection,a quick determination method was established to identify the avian-derived ingredients. The results showed that the selected primers could identify the ingredients of animal origin effectively and quickly. The method was convenient and concise,and could detect the chicken-derived,pigeon-derived,quail-derived ingredients in livestock and poultry food quickly and accurately. | Jingxin ZHANG Yushi GAO Xiujun TANG Yanfeng FAN Mengjun TANG Dawei CHEN Qinglian GE Rong GU | 2016 | Asian Agricultural Research2016，8，12： | 0 |
| 6 | Residual Regularity in Tissues of Layer after Combined Exposure to Lead(Pb)and Cadmium(Cd)显示文摘[Objective]The study was to investigate residual regularity in tissues of layer after combined exposure to lead (Pb)and/or cadmium (Cd).[Method]Lead acetate (100 mg/L)and/or cadmium chloride (50 mg/L)were administered in drinking water to 120 individuals of 40-week-old hyline layers for 8 weeks.[Result]Compared with the Cd group,the Cd content in various tissues,except kidney and femur,in Cd+Pb group all increased,but the difference was not significant (P>0.05).The Cd contents in kidney and femur in Cd group were significantly higher than that in Cd+Pb group.Compared with the group Pb,the Pb contents in breast muscle and kidney in Cd+Pb group decreased significantly (P<0.05);the Pb contents in femur decreased extremely (P<0.01);while the Pb contents in leg muscle,heart and spleen increased significantly (P<0.05);the Pb contents in liver and glandular stomach were almost the same.Compared with the control group,the Cd content in various tissues in Pb group increased significantly (P<0.05);no significant changes were observed for Pb content in Cd group.[Conclusion]Deposit of Pb and Cd in poultry body had complex interaction. | Chen Dawei Liu Yinyin Wang Qianqian Pu Junhua Ma Lina Ge Qinglian Tang Xiujun Gao Yushi | 2016 | Animal Husbandry and Feed Science2016，8，5： | 0 |
| 7 | Complete Sequence Determination and Origin of Mitochondrial DNA Control Region in Dehua Black Chicken显示文摘Primers were designed according to the genomic DNA of red jungle fowl,and the complete sequence of mt DNA D-loop region in Dehua black chicken was obtained.Combined with the D-loop region complete sequence of red jungle fowl in Gen Bank,the maternal origin of Dehua black chicken was explored.The results showed that the complete sequence of mt DNA D-loop region in Dehua black chicken was 1 231 bp,and the average contents of T,C,A and G were33.5%,26.5%,26.7% and 13.3%,respectively.Phylogenetic analysis showed that Dehua black chicken clustered with red jungle fowl (Gallus gallus spadiceus),suggesting Dehua black chicken probably originated from G.g.spadiceus in Yunnan Province or nearby areas. | Lu Junxian Jia Xiaoxu Tang Xiujun Fan Yanfeng Gu Rong Ge Qinglian Gao Yushi | 2016 | Animal Husbandry and Feed Science2016，8，5： | 0 |
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