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| 1 | Two Poplar Glycosyltransferase Genes, PdGATL 1.1 and PdGATL1.2, Are Functional Orthologs to PARVUS/AtGATL 1 in Arabidopsis显示文摘包括 PARVUS/AtGATL1,在 Arabidopsis 的几基因在木聚糖合成被含有。然而,在这多糖是木头的一个主要部件的地方,在木质的植物的木聚糖的生合成糟糕被理解。这里,我们描绘二 Populus 基因, PdGATL1.1 和 PdGATL1.2,最靠近的 orthologs 到 Arabidopsis PARVUS/GATL1 基因关于他们在白杨的基因表示,他们在 Arabidopsis 补充 parvus 变化的亚 cellular 本地化,和他们的能力。包括词法变化,在缺点的救的大多数由 parvus 变化引起了的 parvus 异种的二白杨基因的 Overexpression 折叠木部,并且改变了房间墙 monosaccharide 作文。量的 RTPCR 证明 PdGATL1.1 在开发白杨的木部最强烈被表示。相反, PdGATL1.2 在叶,射击尖端,外皮,韧皮部,和木部更一致地被表示,并且 PdGATL1.2 的抄本水平是比在检验的所有纸巾的 PdGATL1.1 的低得多的。亚 cellular 本地化实验证明这二蛋白质对局部性嗯并且与到这些亚 cellular 分隔空间的标记蛋白质居民比较的 Golgi。我们的数据显示 PdGATL1.1 和 PdGATL1.2 是 PARVUS/GATL1 的功能的 orthologs 并且能在木聚糖合成起一个作用,但是可以也在另外的墙聚合物的合成有角色。 | Yingzhen Kong Gongke Zhou Utku Avci Xiaogang Gu Chelsea Jones Yanbin Yin Ying Xu Michael G. Hahn | 2009 | Molecular Plant2009，2，5： | 6 |
| 2 | Progress on the application of electrochemiluminescence biosensor based on nanomaterials显示文摘Electrogenerated chemiluminescence, also known as electrochemiluminescence, abbreviated ECL, is a new technology combining electrochemistry and chemiluminescence. It is generated by high-energy electrons generated on the surface of the electrode in the emission process of excited state photons formed in the transfer process, and is a perfect combination of electrochemistry and spectroscopy. It not only has the advantages of good environment, high luminosity and wide dynamic range, but also has the characteristics of simple, stable and practical electrochemical methods, and nearly zero background signals. With the rapid development of nanomaterials, due to their unique electrical properties, large specific surface area, good biocompatibility and other characteristics, various nanomaterials have been widely used in the field of biosensors and sensitive detection. This review presented a general description of the research status of four different types of biosensors from the last decade years, summarized the application forms of nanomaterials in ECL biosensor, and outlines the building patterns and application example of the four main types of biosensors. | Zhaoxia Shi Gongke Li Yufei Hu | 2019 | Chinese Chemical Letters2019，30，9： | 6 |
| 3 | Expression patterns of the rice class I metallothionein gene family in response to lead stress in rice seedlings and functional complementation of its members in lead-sensitive yeast cells显示文摘Metallothioneins (MTs) are a group of low molecular mass and cysteine-rich proteins that can chelate heavy-metal ions. In this paper,Northern blot analysis was used to investigate the influence of lead stress on the expression patterns of 10 rice class I MT genes (OsMT-Is) in rice seedlings. With the ex-ception of OsMT-I-3b,the data demonstrate dynamic changes of 9 OsMT-I transcripts in response to Pb2+ treatment in rice seedling roots. Of these genes,transcription of OsMT-I-1a,OsMT-I-1b,OsMT-I-2c,OsMT-I-4a,OsMT-I-4b and OsMT-I-4c increased significantly,while transcription of OsMT-I-2a and OsMT-I-3a increased marginally. In contrast,the expression of OsMT-I-2b was inhibited. Pb2+ induced the expression of 6 OsMT-I genes in seedling shoots,but had no obvious effects on the expression of OsMT-I-1a,OsMT-I-1b,OsMT-I-4a and OsMT-I-4b. All the 10 OsMT-Is had enhanced lead tolerance when heterologously expressed in lead-sensitive yeast mutant cells. These results provide an expression profile of the rice MT gene family in response to Pb2+ stress in rice seedlings and demonstrate in-creased lead tolerance in sensitive yeast mutant cells expressing OsMT-Is. This study lays a foundation for further analysis of the role of the rice MT gene family in respond to Pb2+ stress. | XU YuFeng ZHOU GongKe ZHOU Lu LI YiQin LIU JinYuan | 2007 | Chinese Science Bulletin2007，52，16： | 5 |
| 4 | Arabidopsis NIMIN1,2 mediate redox homeostasis and JA/ET pathways to modulate Botrytis cinerea resistance显示文摘Arabidopsis NIM1-INTERACTING1 (NIMIN1) and NIMIN2 are required for salicylic acid (SA)-dependent resistance against biotrophic pathogens. In this study, we have demonstrated that NIMIN1, 2 are also essential for plant defense response to necrotrophic pathogen Botrytis cinerea. The nimin1 and nimin2 mutants displayed a higher susceptibility against B. cinerea than the wild type, which correlated with a decrease in B. cinerea-induced PDF).2 expression. Mutation in NIMIN1 or NIMIN2 enhanced accumulation of hydrogen peroxide (H_2O_2) with reductions in the activities of three main antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) at the early time (24h) of upon B. cinerea infection. These mutations also resulted in a visible decrease in B.cinerea-induced Ethylene Responsive Factor 1 (ERF1), Octadecanoid-Responsive Arabidopsis AP2/ERF 59 (ORA59), Allene Oxide Cyclase (AOC3), Opda Reductase 3 (OPR3),ACC Synthesis 2 (ACS2) and ACS6 expression, but an advance in MYC2 expression,indicating that NIMIN1,2 are essential for B. cinerea-activated jasmonic acid (JA)/ethylene(ET) biosynthesis and signaling. However, mutation in NIMIN1 or NIMIN2 drastically suppressed JA-, but not ET-activated PDF1.2 expression. Together, these results suggest that NIMIN1,2 may positively control the B. cinerea resistance by mediating redox homeostasis and JA/ET pathways in Arabidopsis. | Dian Wang Junyan Huang Yizhou He Gongke Zhou Shengyi Liu Guohua Chai | 2018 | Oil Crop Science2018，3，1： | 2 |
| 5 | Advances of supercritical fluid chromatography in lipid profiling显示文摘Supercritical fluid chromatography(SFC) meets with great favor due to its high efficiency, low organic solvent consumption, and the specialty for the identification of the isomeric species. This review describes the advances of SFC in targeted and untargeted lipid profiling. The advancement of the SFC instruments and the stationary phases are summarized. Typical applications of SFC to the targeted and untargeted lipid profiling are discussed in detail. Moreover, the perspectives of SFC in the lipid profiling are also proposed. As a useful and promising tool for investigating lipids in vitro and in vivo, SFC will predictably obtain further development. | Yang Yang Yanshan Liang Jina Yang Fengying Ye Ting Zhou Gongke Li | 2019 | Journal of Pharmaceutical Analysis2019，9，1： | 2 |
| 6 | Hybrid field-assisted solid-liquid-solid dispersive extraction for the determination of organochlorine pesticides in tobacco with gas chromatography显示文摘 | ZHOU Ting XIAO Xiaohua LI Gongke | 2012 | Analytical Chemistry2012，84，1： | 1 |
| 7 | 显示文摘 | HU Xiaogang HU Yuling LI Gongke | 2007 | J Chromatogr: A2007，1147，1： | 1 |
| 8 | Study on seafood volatile profile characteristics during storage and its potential use for freshness evaluation by headspace solid phase microextraction coupled with gas chromatography-mass spectrometry显示文摘 | ZHANG Zhuomin LI Gongke LUO Lin | 2010 | Analytica Chimica Acta2010，659，12： | 1 |
| 9 | Genomic insights into salt adaptation in a desert poplar显示文摘 | Ma Tao Wang Junyi Zhou Gongke | 2013 | Nature Commu- nications2013，，： | 1 |
| 10 | Magnetic Ti_(3)C_(2)T_(x)/Fe_(3)O_(4)/Ag substrate for rapid quantification of trace sulfonamides in aquatic products by surface enhanced Raman spectroscopy显示文摘Surface-enhanced Raman scattering(SERS)spectroscopy has been employed as a rapid analysis technology for food security inspection recently.Nowadays,it is still a great challenge to rapidly quantify multiple trace antibiotics potentially abused in aquaculture industry.In this work,a magnetic Ti_(3)C_(2)T_(x)/Fe_(3)O_(4)/Ag substrate was prepared for the development of a reliable rapid SERS quantification method for multiple trace sulfonamides in aquatic products.This magnetic substrate had good uniformity,reproducibility,stability and SERS activity.Moreover,this substrate could integrate the magnetic separation-enrichment and matrix clean-up without cross contamination,which endowed it with good selectivity and antiinterference capability during real sample analysis.The electromagnetic enhancement and chemical enhancement mechanism of this magnetic substrate were studied in detail to reveal its good separationenrichment performance and SERS activity.Finally,a rapid SERS quantification method was established and practically applied for trace phthalic sulfathiazole(PST)and silver sulfadiazine(SSD)in aquatic products by using Ti_(3)C_(2)T_(x)/Fe_(3)O_(4)/Ag magnetic substrates.Trace PST and SSD could be actually detected and quantified as 55.9μg/kg and 64.0μg/kg in aquatic products,respectively.Good recoveries of 83.9%-116%with relative standard deviations(RSDs)of 0.5%-3.2%for PST and 80.2%-102%with RSDs of 1.3%-5.8%for SSD were obtained.This work proposed an efficient and reliable method for rapid quantification of trace multiple sulfonamides in complex aquatic samples during food security inspection. | Zhongning Yu Lu Huang Zhuomin Zhang Gongke Li | 2022 | Chinese Chemical Letters2022，33，8： | 1 |
| 11 | Hydroxyl radical scavenging activity of β-N-oxalyl-L-α, β-diamino propionic acid显示文摘 | Zhou Gongke Kong Yingzhen Cui Kuirong | 2001 | Phytochemistry2001，58，5： | 1 |
| 12 | Study on the compositional differences between transgenic and non-transgenic papaya ( Carica papaya L.)显示文摘 | Zhe Jiao Jianchao Deng Gongke Li Zhuomin Zhang Zongwei Cai | 2010 | Journal of Food Composition and Analysis2010，，6： | 1 |
| 13 | Study on seafood volatile profile characteristics during storage and its potential use for freshness evaluation by headspace solid phase microextraction coupled with gas chromatography-mass spectrometry显示文摘 | ZHANG Zhuomin LI Gongke LUO Lin | 2010 | Anal Chim Acta2010，659，： | 1 |
| 14 | Development of selective and chemically stable coating for stir bar sorptive extraction by molecularly imprinted technique显示文摘 | Yuling Hu Jiawei Li Yufei Hu Gongke Li | 2010 | Talanta2010，，2： | 1 |
| 15 | A GC-MS study of the volatile organic composition of straw and oyster mushrooms during maturity and its relation to antioxidant activity显示文摘 | ZHANG Zhuomin WU Wenwei LI Gongke | 2008 | J Chromatogr Sci2008，46，8： | 1 |
| 16 | Novel magnetic SPE method based on carbon nanotubes filled with cobalt ferrite for the analysis of organochlorine pesticides in honey and tea显示文摘 | Du Zhuo Liu Miao Li Gongke | 2013 | Journal of Sepcnation Science2013，36，20： | 1 |
| 17 | Study of the volatile composition of tomato during storage by a combination sampling method coupled with gas chromatography/mass spectrometry显示文摘 | ZHANG Zhuoming ZENG Dandan LI Gongke | 2008 | J Sci Food Agric2008，88，： | 1 |
| 18 | Fiber-in-tube solid-phase microextraction with molecularly imprinted coating for sensitive analysis of antibiotic drugs by high performance liquid chromatography显示文摘 | Yuling Hu Chaoyong Song Gongke Li | 2012 | Journal of Chromatography A2012，，： | 1 |
| 19 | Preparation and evaluation of propranolol molecularly imprinted solid-phase microextraction fiber for trace analysis of β-blockers in urine and plasma samples显示文摘 | Xiaogang Hu Jialiang Pan Yuling Hu Gongke Li | 2008 | Journal of Chromatography A2008，，： | 1 |
| 20 | 显示文摘 | GongK Du F Xia Z | 2009 | Science2009，323，： | 1 |
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