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| 1 | Aggregation-induced emission (ALE) dye loaded polymer nanoparticles for gene silencing in pancreatic cancer and their in vitro and in vivo biocompatibility evaluation显示文摘 | Rui Hu Chengbin Yang Yucheng Wang Guimiao Lin Wei Qin Qingling Ouyang Wing-Cheung Law Quoc Toan Nguyen Ho Sup Yoon Xiaomei Wang Ken-Tye Yong Ben Zhong Tang | 2015 | Nano Research2015，8，5： | 4 |
| 2 | Biodegradable nanocarriers for small interfering ribonucleic acid (siRNA) co-delivery strategy increase the chemosensitivity of pancreatic cancer cells to gemcitabine显示文摘Ribonucleic acid (RNA) interference (RNAi) therapies are promising cancertreatment modalities that can specifically target abnormal proto-oncogenes, thusimproving the therapeutic effect. For the treatment of pancreatic cancer, targetingone mutant proto-oncogene by RNAi usually does not yield the desired therapeuticefficiency. Both K-ras gene mutations and Notch1 overexpression are commonsymptoms in pancreatic cancer patients, and play a crucial role in pancreaticcancer cell drug resistance. In this study, biodegradable charged polyester-basedvectors (BCPVs) were synthesized for the co-delivery of K-ras and Notch1 smallinterfering ribonucleic acid (siRNA) into MiaPaCa-2 cells (pancreatic cancercell line) to overcome drug resistance to gemcitabine (GEM), a first-line chem-otherapeutic drug used in the clinic. BCPVs could effectively absorb negative siRNAto form a capsule-like structure, prevent siRNA from nuclease digestion in theserum, and promote effective siRNA cell internalization and endosomal escape.Through K-ras and Notch1 gene silencing in MiaPaCa-2 cells, BCPV-siRNAKsiRNAN^tcm nanocomplexes effectively reversed the epithelia-mesenchymaltransition (EMT) in MiaPaCa-2 cells, thereby greatly enhancing the sensitivity ofMiaPaCa-2 cells to GEM. MiaPaCa-2 cell proliferation, migration, and invasionwere effectively inhibited, and cell apoptosis was also significantly enhanced bythe synergistic antitumor effect of BCPV-siRNAK siRNAN^t~l nanocomplexesand GEM. These results suggest that this combination RNAi therapy can be usedto improve cancer cell sensitivity to chemotherapeutic drugs. Specifically, thisnewly develooed strate^v has a great potential for treating pancreatic cancer. | Chengbin Yang Kok Ken Chan Wen-Jen Lin Alana Mauluidy Soehartono Guimiao Lin Huiting Toh Ho Sup Yoon Chih-Kuang Chen Ken-Tye Yong | 2017 | Nano Research2017，10，9： | 3 |
| 3 | An equine disease diagnosis expert system based on improved reasoning of evidence credibility显示文摘In China,there is a troubling shortage of well-trained equine veterinarians,leaving the needs of many equine farmers unmet.This is especially true with respect to the diagnosis of equine diseases.To solve this shortcoming,an equine disease diagnosis expert system was developed.For the aspect of knowledge representation,the structure of equine disease diagnosis knowledge was analyzed using an ontology system.Next,the clinical signs were described using an object-attribute-value(O-A-V)format,and the knowledge representation was then expressed using production rules.With respect to the reasoning mechanism,the weights of the clinical signs and promoted confidence factors(PCF)were combined to express information and rules pertaining to clinical signs with an associated level of uncertainty.The model was established based on improved reasoning of evidence credibility.Finally,using the ASP.Net platform and the SQL Server 2008 database,the equine disease diagnosis expert system based on the B/S structure has been developed,and is capable of reliably diagnosing 40 of the most common equine diseases.A functional evaluation of the system was conducted,and the diagnostic accuracy was observed to be 88%.This study demonstrates a bright prospect for the popularization and application of the system through continuous system maintenance and knowledge-based updates. | Hongyan Gao Guimiao Jiang Xiang Gao Jianhua Xiao Hongbin Wang | 2019 | Information Processing in Agriculture2019，6，3： | 2 |
| 4 | Pegylated phospho- lipid micelle-encapsulated near-infrared PbS quantum dots for in vitro and in vivo bioimaging显示文摘 | Rui H Wing-Cheung L Guimiao L | 2012 | Theranostics2012，2，7： | 1 |
| 5 | Early growth re-sponse protein-1 promoter-mediated synergistic antitumor effect ofhTERTC27 gene therapy and 5 -Flurorouracil on nasopharyngealcarcinoma显示文摘 | Guimiao Lin Marie Chia-Mi Lin Suxia | 2012 | Cancer biotherapy & radiopharmaceuticals2012，27，7： | 1 |
| 6 | Early growth response protein - 1 promoter - mediated synergistic anti - tumor effect of hTERTC27 gene therapy and 5 - Flu- rorouracil on nasopharyngeal carcinoma显示文摘 | Guimiao Lin Marie Chia - Mi Lin Suxia | 2012 | Cancer biother- apy & radiopharmaceuticals2012，27，7： | 1 |
| 7 | Synthetic and immunological studies on the OCT4 immunodominant motif antigen-based anti-cancer vaccine显示文摘Objective:Cancer stem cell is one of the important causes of tumorigenesis as well as a drug target in the treatment of malignant tumor.However,at present,there is no immune vaccine targeting these cells.Octamer-binding transcription factor 4(OCT4),a marker of embryonic stem cells and germ cells,often highly expresses in the early stages of tumorigenesis and is therefore a good candidate for cancer vaccine development.Methods:To identify the optimal carrier and adjuvant combination,we chemically synthesized and linked three different OCT4 epitope antigens to a carrier protein,keyhole limpet hemocyanin(KLH),combined with Toll-like receptor 9 agonist(TLR9).Results:Immunization with OCT4-3+TLR9 produced the strongest immune response in mice.In prevention assays,significant tumor growth inhibition was achieved in BABL/c mice treated with OCT4-3+TLR9(P<0.01).Importantly,the results showed that cytotoxic T lymphocyte activity and the inhibition of tumor growth were enhanced in mice immunized with OCT4-3 combined with TLR9.Meanwhile,multiple cytokines[such as interferon(IFN)-γ(P<0.05),interleukin(IL)-12(P<0.05),IL-2(P<0.01),and IL-6(P<0.05)]promoting cellular immune responses were shown to be greatly enhanced in mice immunized with OCT4-3+TLR9.Moreover,we considered safety considerations in terms of the composition of the vaccines to help facilitate the development of effective next-generation vaccines.Conclusions:Collectively,these experiments demonstrated that combination therapy with TLR9 agonist induced a tumor-specific adaptive immune response,leading to the suppression of primary tumor growth in testis embryonic carcinoma. | Tingting Chen Kan Liu Jiangyao Xu Tianying Zhan Maixian Liu Li Li Zhiwen Yang Shuping Yuan Wenyi Zou Guimiao Lin Dennis ACarson Christina CNWu Xiaomei Wang | 2020 | Cancer Biology & Medicine2020，17，1： | 0 |
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