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| 1 | Anti-P-selectin lectin-EGF domain monoclonal antibody inhibits the maturation of human immature dendritic cells显示文摘Dendritic cells(DCs) are professinal antigen-presenting cells with the ability to initiate primary Tcell responses. While it iswell known that inflammatory stimuli induce the functional maturation of immature DCs, whether adhesion molecule selectins regulate DCmaturation is poorly understood. Using anti-P-selectin lectin-EGF domain monoclonal antibody (PsL-EGFmAb) that blocks the adhesionof P-, E-, and L-selectin, we demonstrate herein that selectins play important role in stimulating functional maturation of immature DCs.Immature DCs are generated from human cord blood CD34+hematopoietic stem/progenitor cells that were cultured in the presence of stemcell factor, Fms-like tyrosine-kinase-3 ligand, granulocyte-macrophage colony stimulating factor, and transform growth factor-β1. Whenstimulated with tumor necrosis factor-alpha(TNF-α), immature DCs differentiated into mature DCs, producing increased levels of costim-ulatory molecules and interleukin (IL)-12 and obtaining the ability to potently activate naive Tcells. Interestingly, in contrast to matureDCs derived from TNF-α-induced immature DC cultures without PsL-EGFmAb, immature DCs treated with PsL-EGFmAb for7 days werecompletely blocked their maturation, as evidenced by decreased expression of costimulatory molecules CD80, CD86, and CD83, inhibi-ted production of IL-12, and inability to activate naive Tcellsin vitro. Thus, blockade of selectins using PsL-EGFmAb will prove to bea valuable tool for the study of the molecuar mechanisms of DC maturation, as well as for the prevention and treatment of DC-mediated au-toimmunity. | Tong Zhou Yanyu Zhang Guizhi Sun Yumei Zhang Dongqing Zhang Yapeng Zhao Nan Chen | 2006 | 上海交通大学学报(医学版)2006，26，5： | 5 |
| 2 | Valsartan Inhibited the Accumulation of Dendritic Cells in Rat Fibrotic Renal Tissue显示文摘为了在到 tubulointerstitial 纤维变性的老鼠残余肾和它的贡献观察树枝状的房间(DC ) 的累积,在 DC 上的 valsartan 的影响下面,一个老鼠残余肾模型被小计肾切除术建立。四个试验性的组被包括:正常,假冒,模型(SNx ) 和这个组与 Valsartan (SNxV ) 对待。老鼠分别地在星期 1,4 和 12 点被打死。CD1a+CD80+ DC 是由两倍 immunostaining 方法的 assayed,图象与 Axioplan 2 显微镜学被分析。P-selectin, TGF-1, -SMA, 骨胶原和 fibronectin 的表情被 immunohistochemistry 或 semiquantitative RT-PCR 分析,并且 tubulointerstitial firosis (TIF ) 的水平被获得。CD1a+CD80+ DC 逐渐地在肾的小管, interstitium 和容器之中被增加,特别在在在星期 12 点的模型组的 DC 的 interstitium,和数字在在星期 1 或 4 点的模型组是多于那的大部分。在 tubulointerstitial 区域和 TIF 的度的 P-selectin, TGF-1, -SMA, 骨胶原和 fibronectin 的表情在星期 12 点在模型组实质地被增加。在 interstitium 的 DC 的累积很好与肾的功能和 tubulointerstitial 纤维变性的前进的损失被联系。Valsartan 处理禁止了 DC 的本地累积并且稀释了肾的 tubulointerstitial 损坏。本地 DC 累积与跟随肾的脱离的 tubulointerstitial 纤维变性和肾的机能障碍有关。对血管收缩素的封锁可能是稀释肾的免疫煽动性的损害的一个有势力方法。 | Kaiyin Wu Tong Zhou Guizhi Sun Weiming Wang Yumei Zhang Yanyun Zhang Li Hao Nan Chen | 2006 | Cellular & Molecular Immunology2006，3，3： | 4 |
| 3 | Effects of Bovine Embryos at Different Developmental Stages on Bisection Effect显示文摘[Objectives]This study was conducted to explore the effects of embryos at different developmental stages on the bisection effect of embryos,improve the efficiency of bovine embryo bisection,and facilitate the application of embryo bisection technology in cattle breeding.[Methods]The effects of two different bisection solutions on the bisection of morulae and blastocysts in vitro were explored.The morulae and blastocysts produced in vitro from cattle that developed to the 6th to 8th d were bisected by hands,and demi-embryos were cultured in vitro.Their development was observed.[Results]Morulae were bisected in PBS solution and PBS+0.2 mol/L sucrose,and the success rates of bisection were 50%and 95.2%,respectively.The success rate of bisecting morulae in PBS+0.2 mol/L sucrose was significantly higher than that in PBS(P<0.05),while the development rate of the bisected demi-embryos had no significant difference between the two(53.3%,52.4%)(P>0.05).The success rates of blastocyst bisection in PBS solution and PBS+0.2 mol/L sucrose were 51.6%and 95.1%,respectively.The success rate of blastocyst bisection in PBS+0.2 mol/L sucrose was significantly higher than that in PBS(P<0.05),while the development rate of the bisected demi-embryos had no significant difference between the two(50.0%,56.4%)(P>0.05).[Conclusions]There were no significant differences between the success rates of bisecting bovine morulae and blastocysts in PBS+0.2 mol/L sucrose,which were both significantly better than those in pure PBS bisection solution,proving that PBS+0.2 mol/L sucrose bisection solution is suitable for bovine embryo bisection. | Guizhi TONG Yongsheng HAN Hongbao WANG Xintao LI Weixia WANG Xueying SONG | 2021 | Agricultural Biotechnology2021，10，5： | 0 |
| 4 | Ge Film Contacts to Radio Frequency Sputtered Y-Ba-Cu-O Superconductor Film显示文摘The semiconductor-superconductor con tact system of Ge/Y-Ba-Cu-O(YBCO)/SrTiO_(3) has been successfully fabricated by two steps of deposition.The YBCO film in the contact of Ge/YBCO did not lose its superconductivity and its T_(c)(on set)did not degrade as well.The top layer of Ge was polycrystalline with preferred orientation corresponding to the epitaxial structure of the underlayer of YBCO. | ZHANG Bei QIAN Yi TONG Litai CHEN Haiying WANG Guizhi QIAO Yongping XING Miaoxiang FENG Qingrong LIU Zunxiao LAN Jian DAI Yuandong | 1991 | Chinese Physics Letters1991，8，9： | 0 |
| 5 | Propagation Techniques of Ewes at Early Age显示文摘The integration of hormone induction of young ewes,live oocyte collection,in-vitro fertilization and embryo transfer( JIVET),as a major breakthrough in livestock breeding technology,not only solves the problem of low oocytes obtained from adult ewes by hormone induction in production practice,but also significantly improves production efficiency. This technology can reduce the generation interval of cattle and sheep and speed up the acquisition of genes in genetic breeding. The application of this technology can help accelerate the cultivation process,shorten the generation interval,promote the industrialization and improve the economic benefits of beef cattle breed. This paper provides a reference for the research and application of new breeding and propagation techniques of beef cattle. | Caihong HAO Guizhi TONG Xueying SONG Likun WANG Zhenhua JIN Jiahou WANG Wenkai GUO Li LI | 2023 | Agricultural Biotechnology2023，12，1： | 0 |
| 6 | Molecular Markers of Fat and Inosinic Acid Content in Sheep Muscle and Application显示文摘This study was conducted to speed up the process of improving the quality of lamb breeds in China,and to solve the problems that restrict the development of lamb breeds in China,such as low quality of lamb breeds,low meat production rate,and poor meat quality.By studying molecular markers of fat content in sheep muscles,we searched for polymorphic loci related to fat content in sheep muscles on the ANGPTL4 gene.Among the 9 polymorphic loci retrieved,ANGPTL4 was found.Two polymorphic sites,C724 A and A601 G,were significantly associated with intramuscular fat in mutton.In summary,these two molecular markers can be used as a molecular marker reference when breeding high-quality meat or hair and meat using German Merino sheep breeds. | Guizhi TONG Xuefeng LIU Xintao LI Gui ZHU Weixia WANG Long HAI | 2020 | Agricultural Biotechnology2020，9，2： | 0 |
| 7 | High-efficiency Embryo Cryopreservation Technology for Mutton Sheep显示文摘Embryo cryopreservation is an important part of embryo biotechnology.It plays a key role in the development of quality animal husbandry.However,the effect of in-vivo(in-vitro)embryo freezing of cattle and sheep in China has not reached an expected level,which limits the commercial application of embryo transfer technology.The technical bottleneck that needs to be broken through is that embryos are subject to mechanical damage and protective agent toxicity during freezing and thawing.This paper summarized the successful application of plant antifreeze protein in vitrification preservation of pig embryos,which makes this technology widely used in animal husbandry production,and is of great significance in accelerating the large-scale breeding of improved livestock and saving endangered animals. | Caihong HAO Guizhi TONG Xueying SONG Xintao LI Likun WANG Zhenhua JIN Jiahou WANG Wenkai GUO Ping LI Li LI Yan ZHANG | 2023 | Agricultural Biotechnology2023，12，2： | 0 |
| 8 | Research on Key Technology of Efficient Production of Bovine Embryos显示文摘[Objectives]This study was conducted to explore the effects of three different splitting fluids on the bisection effects of bovine morulae and blastocysts.[Methods]With the help of a micromanipulator,the morulae and blastocysts that were routinely produced in the body from the 6 th to the 8 th d were divided into half-embryos in vitro to observe their development,and the half-embryos with a restored morphology and a blastocyst trophoblast were selected.[Results]The success rates of bisection of morulae in PBS+0.2 mol/L sucrose and PBS+5%PVP were significantly higher than that in PBS(P<0.05),and the values in the three liquids were 95.7%,97.2% and 50%,respectively,while the blastocyst development rates and of half-embryos and the pregnancy rates of transplantation were not significantly different(P>0.05).When blastocysts were bisected in PBS+0.2 mol/L sucrose and PBS+5% PVP,the success rates of bisection were significantly higher than that of PBS(P<0.05),and the values in the three liquids were 96.8%,95.5% and 50.0%,respectively,while the development rates of half-embryos and the pregnancy rates of transplantation were not significantly different(P>0.05).The development rate of half-embryos bisected from blastocysts was significantly higher than that of morulae.[Conclusions]This study improves the efficiency of bovine embryo bisection,which is beneficial to the application of embryo bisection technology in cattle breeding. | Guizhi TONG Hongguang WANG Yongsheng HAN Xintao LI Weixia WANG Bin SONG | 2020 | Agricultural Biotechnology2020，9，4： | 0 |
| 9 | Study on the Relationship Between CAPN Gene Peptide and Fat Content in Sheep Muscle显示文摘[Objectives] This study was conducted to speed up the process of improving the quality of lamb breeds in China,and to solve the problems that restrict the development of lamb breeds in China,such as low quality of lamb breeds,low meat production rate,and poor meat quality. [Methods]By studying molecular markers of fat content in sheep muscle,the CAPN gene was used to find polymorphic sites related to fat content in sheep muscle,and the correlation between two polymorphic sites C724 A and A601G on CAPN1 and CAPN2 and fat content in sheep muscle was analyzed. [Results] The two polymorphic sites,C724 A on CAPN1 and A601G on CAPN2 were significantly related to intramuscular fat in mutton. [Conclusions] These two molecular markers can be used as a molecular marker reference when breeding high-quality meat or hair and meat using German Merino sheep breeds. | Guizhi TONG Xuefeng LIU Gui ZHU Xintao LI Long HAI Weixia WANG | 2020 | Agricultural Biotechnology2020，9，3： | 0 |
| 10 | Technical Measures for Rapid Propagation of Improved Beef Cattle显示文摘Based on early weaning in calves,hormone induction in young calves,intravital egg collection,in-vitro fertilization and embryo transfer which serve as major breakthroughs in livestock breeding technology,the techniques of hormone induction and in-vitro fertilization were integrated,not only to solve the problem of obtaining oocytes by hormone induction in adult cows in production practice,but also to obviously improve production efficiency.The application of this technology can help accelerate the cultivation process,shorten the generation interval,promote the industrialization and improve the economic benefits of beef cattle breed.This paper provides a reference for the research and application of new breeding and propagation techniques of beef cattle. | Caihong HAO Guizhi TONG Xueying SONG Xinying DING Likun WANG Zhenhua JIN Xintao LI Haiyang YOU Guowang CHEN Jingdong NAN Jiansheng ZHANG | 2022 | Agricultural Biotechnology2022，11，6： | 0 |
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