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| 1 | Re-detectable positive SARS-CoV-2 RNA tests in patients who recovered from COVID-19 with intestinal infection显示文摘Dear Editor,Severe acute respiratory syndrome coronavirus 2(SARSCoV-2),a novel coronavirus that causes Coronavirus Disease 2019(COVID-19)(Yang and Wang,2020),has spread to more than 200 countries and infected more than 9,000,000 people as of Jun 2020.Tens of thousands of patients with COVID-19 have recovered and been discharged from hospital.However,there are reports of recovered patients who subsequently tested positive for SARSCoV-2 after discharge(re-detectable positive,RP)(An et al.,2020;Lan et al.,2020),and this has led to increasing focus on the mechanism(s)underlying RP. | Wanyin Tao Xiaofang Wang Guorong Zhang Meng Guo Huan Ma Dan Zhao Yong Sun Jun He Lianxin Liu Kaiguang Zhang Yucai Wang Jianping Weng Xiaoling Ma Tengchuan Jin Shu Zhu | 2021 | Protein & Cell2021，12，3： | 1 |
| 2 | Targeting TRPV1-mediated autophagy attenuates nitrogen mustard-induced dermal toxicity显示文摘Nitrogen mustard(NM)causes severe vesicating skin injury,which lacks effective targeted therapies.The major limitation is that the specific mechanism of NM-induced skin injury is not well understood.Recently,autophagy has been found to play important roles in physical and chemical exposure-caused cutaneous injuries.However,whether autophagy contributes to NM-induced dermal toxicity is unclear.Herein,we initially confrmed that NM dose-dependently caused cell death and induced autophagy in keratinocytes.Suppression of autophagy by 3-methyladenine,chloroquine,and baflomycin A1 or ATG5 siRNA attenuated NM-induced keratinocyte cell death.Furthermore,NM increased transient receptor potential vanilloid 1(TRPV1)expression,intracellular Ca2+content,and the activities of Car+/calmodulin-dependent kinase kinaseβ(CaMKKB),AMP-activated protein kinase(AMPK),unc-51-like kinase 1(ULK1),and mammalian target of rapamycin(mTOR).NM-induced autophagy in keratinocytes was abolished by treatment with inhibitors of TRPV1(capsazepine),CaMKKβ(STO-609),AMPK(compound C),and ULK1(SBI-0206965)as well as TRPV1,CaMKKB,and AMPK siRNA transfection.In addition,an mTOR inhibitor(rapamycin)had no signifcant effect on NM-stimulated autophagy or cell death of keratinocytes.Finally,the results of the in vivo experiment in NM-treated skin tissues were consistent with the findings of the in vitro experiment.In conclusion,NM-caused dermal toxicity by overactivating autophagy partially through the activation of TRPV1-Ca2+-CaMKKB-AMPK-ULK1 signaling pathway.These results suggest that blocking TRPV1-dependent autophagy could be a potential treatment strategy for NM-caused cutaneous injury. | Mingliang Chen Xunhu Dong Haoyue Deng Feng Ye Yuanpeng Zhao Jin Cheng Guorong Dan Jiqing Zhao Yan Sai Xiuwu Bian Zhongmin Zou | 2021 | Signal Transduction and Targeted Therapy2021，6，2： | 0 |
| 3 | Metal-organic framework nanofilm enhances serum metabolic profiles for diagnosis and subtype of cardiovascular disease显示文摘Cardiovascular disease(CVD)is a global health problem and is thought to be responsible for almost half of all deaths in the world.Nevertheless,currently available diagnostic methods for CVD are strongly depended on clinical observation and monitoring,which commonly result in false diagnosis.Herein,an attractive strategy of a metal-organic framework(MOF)nanofilm-based laser desorption/ionization mass spectrometry(LDI-MS)was developed for enhancing serum metabolic profiling,which could provide precise diagnosis and molecular subtyping of CVD.The porous MOF nanofilm fabricated on indium-tin oxide(ITO)glass possessed enhanced ionization efficiency and size-exclusion effect,which endowed it as substrate with high sensitivity and selectivity for serum metabolites.Furthermore,the MOF nanofilm with uniform surface and high orientation provided high-quality and high-reproducibility serum metabolic profiles(SMPs)without any tedious pretreatment.Further analysis of extracted serum metabolic fingerprints could successfully distinguish patients with CVD from healthy controls and also differentiate two major subtypes of CVD.This work not only extends the application of MOF nanofilm as an attractive MS probe,but also provide an alternative way for precise diagnosis of CVD in molecular level. | Dan Ouyang Zhihua Fu Guorong Li Chao Zhong Jiahao Yuan Huan Huang Juan Lin Gang Xu Zian Lin | 2023 | Chinese Chemical Letters2023，34，6： | 0 |
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