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| 1 | Alcoholic liver disease:Utility of animal models显示文摘Alcoholic liver disease(ALD) is a major cause of acute and chronic liver injury. Extensive evidence has been accumulated on the pathological process of ALD during the past decades. However, effective treatment options for ALD are very limited due to the lack of suitable in vivo models that recapitulate the full spectrum of ALD. Experimental animal models of ALD, particularly rodents, have been used extensively to mimic human ALD. An ideal animal model should recapitulate all aspects of the ALD process, including significant steatosis, hepatic neutrophil infiltration, and liver injury. A better strategy against ALD depends on clear diagnostic biomarkers, accurate predictor(s) of its progression and new therapeutic approaches to modulate stop or even reverse the disease. Numerous models employing rodent animals have been established in the last decades to investigate the effects of acute and chronic alcohol exposure on the initiation and progression of ALD. Although significant progress has been made in gaining better knowledge on the mechanisms and pathology of ALD, many features of ALD are unknown, and require further investigation, ideally with improved animal models that more effectively mimic human ALD. Although differences in the degree and stages of alcoholic liver injury inevitably exist between animal models and human ALD, the acquisition and translational relevance will be greatly enhanced with the development of new and improved animal models of ALD. | Arantza Lamas-Paz Fengjie Hao Leonard J Nelson Maria Teresa Vázquez Santiago Canals Manuel Gómez del Moral Eduardo Martínez-Naves Yulia A Nevzorova Francisco Javier Cubero | 2018 | World Journal of Gastroenterology2018，24，45： | 26 |
| 2 | Autophagy inhibition by chloroquine sensitizes HT-29 colorectal cancer cells to concurrent chemoradiation显示文摘AIM:To investigate whether the inhibition of autophagy by chloroquine(CQ)sensitizes rectal tumors to radiation therapy(RT)or concurrent chemoradiation(chemoRT).METHODS:In vitro,HCT-116 and HT-29 colorectal cancer(CRC)cell lines were treated as following:(1)PBS;(2)CQ;(3)5-fluorouracil(5-FU);(4)RT;(5)CQ and RT;(6)5-FU and RT;(7)CQ and 5-FU;and(8)5-FU and CQ and RT.Each group was then exposed to various doses of radiation(0-8 Gy)depending on the experiment.Cell viability and proliferative capacity were measured by3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)and clonogenic assays.Clonogenic survivalcurves were constructed and compared across treatment groups.Autophagy status was determined by assessing the LC3-Ⅱto LC3-Ⅰratio on western blot analysis,autophagosome formation on electron microscopy and identification of a perinuclear punctate pattern with GFPlabeled LC3 on fluorescence microscopy.Cell cycle arrest and cell death were evaluated by FACS and AnnexinⅤanalysis.All experiments were performed in triplicate and statistical analysis was performed by the student’s t test to compare means between treatment groups.RESULTS:RT(2-8 Gy)induced autophagy in HCT-116and HT-29 CRC cell lines at 4 and 6 h post-radiation,respectively,as measured by increasing LC3-Ⅱto LC3-Ⅰratio on western blot.Additionally,electron microscopy demonstrated autophagy induction in HT-29 cells24 h following irradiation at a dose of 8 Gy.Drug treatment with 5-FU(25μmol/L)induced autophagy and the combination of 5-FU and RT demonstrated synergism in autophagy induction.CQ(10μmol/L)alone and in combination with RT effectively inhibited autophagy and sensitized both HCT-116 and HT-29 cells to treatment with radiation(8 Gy;P<0.001 and 0.00001,respectively).Significant decrease in clonogenic survival was seen only in the HT-29 cell line,when CQ was combined with RT at doses of 2 and 8 Gy(P<0.5 and P=0.05,respectively).There were no differences in cell cycle progression or Annexin V staining upon CQ addition to RT.CONCLUSION:Autophagy inhibition by CQ increases CRC cell sensitivity to concurrent treatment with 5-FU and RT in vitro,suggesting that addition of CQ to chemoRT improves CRC treatment response. | Caitlin A Schonewolf Monal Mehta Devora Schiff Hao Wu Bruce G Haffty Vassiliki Karantza Salma K Jabbour | 2014 | World Journal of Gastrointestinal Oncology2014，6，3： | 12 |
| 3 | Engineering osteoarthritic cartilage model through differentiating senescent human mesenchymal stem cells for testing disease-modifying drugs显示文摘Significant cellular senescence has been observed in cartilage harvested from patients with osteoarthritis(OA).In this study,we aim to develop a senescence-relevant OA-like cartilage model for developing disease-modifying OA drugs(DMOADs).Spe-cifically,human bone marrow-derived mesenchymal stromal cells(MSCs)were expanded in vitro up to passage 10(P10-MSCs).Following their senescent phenotype formation,P10-MSCs were subjected to pellet culture in chondrogenic medium.Results from qRT-PCR,histology,and immunostaining indicated that cartilage generated from P10-MSCs displayed both senescent and OA-like phenotypes without using other OA-inducing agents,when compared to that from normal passage 4(P4)-MSCs.Interestingly,the same gene expression differences observed between P4-MSCs and P10-MSC-derived cartilage tissues were also observed between the preserved and damaged OA cartilage regions taken from human samples,as demonstrated by RNA sequencing data and other analysis methods.Lastly,the utility of this senescence-initiated OA-like cartilage model in drug development was assessed by testing several potential DMOADs and senolytics.The results suggest that pre-existing cellular senescence can induce the generation of OA-like changes in cartilage.The P4-and P10-MSCs derived cartilage models also represent a novel platform for predicting the efficacy and toxicity of potential DMOADs on both preserved and damaged cartilage in humans. | Ning Wang Yuchen He Silvia Liu Meagan J.Makarcyzk Guanghua Lei Alexander Chang Peter G Alexander Tingjun Hao Anne-Marie Padget Nuria de Pedro Tsapekos Menelaos Hang Lin | 2022 | Science China(Life Sciences)2022，65，2： | 5 |
| 4 | An essential role for TAK1 in the contact hypersensitivity response显示文摘Contact hypersensitivity(CHS)is a delayed-type hypersensitivity that can be induced by haptens,such as 2,4-dinitrofluorobenzene(DNFB).Innate and adaptive immunities are both important for the development of CHS.To treat CHS-related diseases,such as allergic contact dermatitis,a disease prevalent in industrialized countries,ways of interfering with improper immune function during CHS responses need to be identified.Transforming growth factor-b-activated kinase-1(TAK1),a member of mitogen-activated protein kinase kinase kinase family,is important for both innate and adaptive immunities.We thus hypothesized that the CHS response could be inhibited by interfering with TAK1 activity.Using a mouse model in which TAK1 deletion can be locally induced,we observed that TAK deficiency led to an impaired CHS response and was associated with defective T-cell expansion,activation and interferon(IFN)-c production.In addition,we investigated the effect of deleting TAK1 specifically in dendritic cells(DC)on the CHS response.We found that when TAK1 is deficient in DC,the CHS response was abolished and hapten-elicited T-cell responses were defective.Collectively,this study demonstrates an essential role of TAK1 in the induction of CHS and suggests that targeting TAK1 could be a viable approach to treat CHS. | Yan G Zhao Yunqi Wang Weidong Hao Yisong Y Wan | 2011 | Cellular & Molecular Immunology2011，8，4： | 4 |
| 5 | Rice monitoring and production estimation using multitemporal RADARSAT显示文摘 | Yun Shao Xiangtao Fan Hao Liu Jianhua Xiao S Ross B Brisco R Brown G Staples | 2001 | Remote Sensing of Environment2001，，3： | 3 |
| 6 | 基于工作地点的分组干预对高血压控制的影响:一项随机临床研究显示文摘基于工作地点的干预可能为一种管理高血压的有效方法。但是目前缺少针对中国工作人群高血压控制情况的研究。本研究旨在评估基于工作地点的多组分别干预策略对改善血压控制的影响。研究设计及受试者:研究者在2013年1月至2014年12月于中国20个城区的60个工作地点进行了一系列高血压管理的随机临床试验。工作地点随机分为干预组(n=40)和对照组(n=20)。每个工作地点的受试者都需要完成一项横断面调查。 | 袁源(摘译) 叶鹏(审校) Wang Z Wang X Shen Y Li S Chen Z Zheng C Kang Y Jiang L Hao G Chang C Gao R | 2020 | 中华高血压杂志2020，28，6： | 3 |
| 7 | Polymorphisms in DNA base excision repair genes ADPRT and XRCC1 and risk of lung cancer显示文摘 | Zhang XM Miao XP Liang G Hao BT Wang YG Tan W Li Y Guo YL He FC Wei QY Lin DX | 2006 | 中国生物学文摘2006，20，4： | 2 |
| 8 | Rapid phenotypic evolution with shallow genomic differentiation during early stages of high elevation adaptation in Eurasian Tree Sparrows显示文摘Known as the‘third polar region’,the Qinghai-Tibet Plateau represents one of the harshest highland environments in the world and yet a number of organisms thrive there.Previous studies of birds,animals and humans have focused on well-differentiated populations in later stages of phenotypic divergence.The adaptive processes during the initial phase of highland adaptation remain poorly understood.We studied a human commensal,the Eurasian Tree Sparrow,which has followed human agriculture to the Qinghai-Tibet Plateau.Despite strong phenotypic differentiation at multiple levels,in particular in muscle-related phenotypes,highland and lowland populations show shallow genomic divergence and the colonization event occurred within the past few thousand years.In a one-month acclimation experiment investigating phenotypic plasticity,we exposed adult lowland tree sparrows to a hypoxic environment and did not observe muscle changes.Through population genetic analyses,we identified a signature of polygenic adaptation,whereby shifts in allele frequencies are spread across multiple loci,many of which are associated with muscle-related processes.Our results reveal a case of positive selection in which polygenic adaptation appears to drive rapid phenotypic evolution,shedding light on early stages of adaptive evolution to a novel environment. | Yanhua Qu Chunhai Chen Ying Xiong Huishang She Yong E Zhang Yalin Cheng Shane DuBay Dongming Li Per G P Ericson Yan Hao Hongyuan Wang Hongfeng Zhao Gang Song Hailin Zhang Ting Yang Chi Zhang Liping Liang Tianyu Wu Jinyang Zhao Qiang Gao Weiwei Zhai Fumin Lei | 2020 | National Science Review2020，7，1： | 2 |
| 9 | Difference of planktonic ciliate communities of the tropical West Pacific, the Bering Sea and the Arctic Ocean显示文摘Ciliates are important components in planktonic food webs,but our understanding of their community structures in different oceanic water masses is limited.We report pelagic ciliate community characteristics in three seas:the tropical West Pacific,the Bering Sea and the Arctic Ocean.Planktonic ciliate abundance had'bimodal-peak','surface-peak'and'DCM(deep chlorophyll a maximum layer)-peak'vertical distribution patterns in the tropical West Pacific,the Bering Sea and the Arctic Ocean,respectively.The abundance proportion of tintinnid to total ciliate in the Bering Sea(42.6%)was higher than both the tropical West Pacific(7.8%)and the Arctic Ocean(2.0%).The abundance proportion of small aloricate ciliates(10–20μm size-fraction)in the tropical West Pacific was highest in these three seas.The Arctic Ocean had higher abundance proportion of tintinnids in larger LOD(lorica oral diameter)size-class.Proportion of redundant species increased from the Arctic Ocean to the tropical West Pacific.Our result provided useful data to further understand ecology roles of planktonic ciliates in different marine habitats. | Chaofeng Wang Haibo Li Zhiqiang Xu Shan Zheng Qiang Hao Yi Dong Li Zhao Wuchang Zhang Yuan Zhao Gérald Grégori Tian Xiao | 2020 | Acta Oceanologica Sinica2020，39，4： | 2 |
| 10 | Radiative heat transfer and thermal characteristics of Fe-based oxides coated SiC and Alumina RPC structures as integrated solar thermochemical reactor显示文摘This paper investigated radiative heat transfer and thermal characteristics of Fe-based oxides coated SiC and Alumina reticulated porous ceramic structures as integrated solar thermochemical reactor.High-flux solar radiation absorption and axial temperature distribution in the ceramic foams reactor were analyzed by adopting surface-to-surface radiation model coupled to the P1approximation for radiation heat transfer.The radiative heat transfer and thermal characteristics of different foam-type RPC structures,including SiC,CeO_2,FeAl_2O_4,NiFeAlO_4,Fe_3O_4/SiC,and NiFe_2O_4/SiC were evaluated.The mass flow rate and foam structural parameters,including the permeability,pore mean cell size,and extinction coefficients have significantly affected the axial temperature distribution,pressure drop,heat transfer,and fluid flow.Integrated porous structure to the solar receiver could maximize the incorporation of redox powder in the reacting medium,lower the pressure drop,and enhance the thermal performance of the thermochemical reacting system.SiC structure was the candidate materials in the case where more heat flux and high axial temperature distribution is needed.However,Fe-based oxide coated Al_2O_3structure could be considered regarding the heat transfer enhancement along with the catalyst activity of oxygen carriers for solar thermochemical reacting system performance. | GUENE LOUGOU Bachirou SHUAI Yong PAN RuMing CHAFFA Gédéon AHOUANNOU Clément ZHANG Hao TAN HePing | 2018 | Science China(Technological Sciences)2018，61，12： | 2 |
| 11 | Effect of Prior Deformation on Aging Process in a Cu-30Ni-25Fe Spinodal Alloy显示文摘The efFect of prior deformation on the mechanical properties and microstructural evolution ofaging process in a Cu-30Ni-25Fe (in at. pct) alloy has been studied by tensile test, hardness measurement. SEM and optical microscopy analysis. The results show that spinodal decomposition and recovery process interact on each other when aged at low temperature (600℃),and most of the defects induced by cold-work did not disappear in 100 h. When aged at high temperature (850℃), the discontinuous coarsening of the spinodal products takes place. The increase in yield strength and elongation of the sample aged at 850℃ for 5 h after deformation mainly results from the submicrometer grains produced by discontinous coarsening of | Hao, XJ Li, HX Zhao, G Hao, SM | 1999 | Journal of Materials Science & Technology1999，15，6： | 2 |
| 12 | Toughening modification of PS with n-BA/MMA/styrene coreshell structured copolymer from emulsifier-free emulsion polymerization 显示文摘 | Guo T Y Tang G L Hao G J | 2003 | Journal of Applied Science2003，90，： | 1 |
| 13 | Diffusion with hydrolysis and ion association in aqueous solutions of beryllium sulfate 显示文摘 | Hao Ling Lu Ruanhui Derek G L | 1996 | Journal of Solution Chemistry1996，25，3： | 1 |
| 14 | Effectiveness of first eight methadone maintenance treatment clinics in China显示文摘 | Pang L Hao Y Mi G | 2007 | AIDS2007，21，8： | 1 |
| 15 | Anthropogenic mercury emissions in China显示文摘 | Streets D G Hao J Wu Y | 2005 | Atmospheric Environment2005，，39： | 1 |
| 16 | Low temperature selective catalytic reduction of NOx with NH3 over amorphous MnOx catalysts prepared by three methods显示文摘 | Tang X L Hao J M Xu W G | 2007 | Catalysis Communications2007，8，： | 1 |
| 17 | LMI approach L2-gain analysis and control synthesis of uncertain switched systems 显示文摘 | Xie D Wang L Hao F Xie G | 2004 | IEE Proc-Control Theory and applications (S1350-2379)2004，151，1： | 1 |
| 18 | Highly pathogenic porcine reproductive and respiratory syndrome, China 显示文摘 | Tong G Z Zhou Y J Hao X F | 2007 | Emerg Infect Dis2007，13，9： | 1 |
| 19 | Induction and expression of T4 lysozyme gene in Pichia pastoris显示文摘 | Hao W J Li G Q Xu M Y | 2007 | Journal of Chinese Phar- maceutical Sciences2007，16，： | 1 |
| 20 | In situ electron paramagnetic resonance (EPR) study of surface oxygen species on Au/ZnO catalyst for low-temperature carbon monoxide oxidation显示文摘 | Hao Z Fen L Lu G Q et ol | 2001 | Appl Catal A2001，213，2： | 1 |
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