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| 1 | Cucurbitacin B induces rapid depletion of the G-actin pool through reactive oxygen species-dependent actin aggregation in melanoma cells显示文摘Cucurbitacin B (崽) ,从 Cucurbitaceae 植物孤立的 triterpenoid 混合物,作为一个有希望的反癌症代理人被报导了,还它的行动机制仍然是争论的。在这研究,我们在鼠科的 B16F10 黑瘤房间探索了崽的潜在的机制。反增长和反侵略效果在有教养的房间被估计,并且在 vivo 反肿瘤,活动在一个鼠科的下的黑瘤模型被评估。流动 cytometry 被采用分析细胞周期分发和反应的氧种(ROS ) 层次。肌动朊层次被西方的污点分析决定,并且微分表示蛋白质的侧面被一条量的 proteomic 途径识别。结果证明那崽在房间增长,殖民地形成,以及移植和黑瘤房间的侵略潜力上施加了禁止的效果。下的黑瘤的生长显著地在当时,与崽对待的老鼠被禁止与控制相比组。而且,崽治疗引起了快速的房间膜 blebbing 和变丑,并且导致了 G2/M-phase 拘捕和 multiploid 房间的形成。尤其是, G 肌动朊水池很快被弄空,肌动朊总数在崽处理以后快速被形成。很多细胞骨架规章的蛋白质是调整的差别。ROS 生产的阻塞显著地减少了 G 肌动朊弄空能力和崽的反肿瘤活动。这些调查结果显示那崽在黑瘤房间通过 ROS 依赖的肌动朊聚集导致 G 肌动朊水池的快速的弄空,它可以部分至少说明它的反肿瘤活动。 | Yanting Zhang Dongyun Ouyang Lihui Xu Yuhua Ji Qingbing Zha Jiye Cai Xianhui He | 2011 | Acta Biochimica et Biophysica Sinica2011，43，7： | 19 |
| 2 | Inhibitory Effects of Berberine on the Activation and Cell Cycle Progression of Human Peripheral Lymphocytes显示文摘The immunosuppressive property of berberine, an isoquinoline alkaloid, has been well documented, but the mechanism of its action on lymphocytes has not been completely elucidated. The present study is to investigate the effect of berberine on the activation and proliferation of lymphocytes, in particular T lymphocytes. Whole peripheral blood from healthy donors was stimulated with phytohemagglutinin (PHA) alone or phorbol dibutyrate (PDB) plus ionomycin, and the expression of CD69 and CD25 on T lymphocytes was evaluated with flow cytometry.The distribution of cell cycles and cell viability were analyzed by staining with propidium iodide (PI) and 7-aminoactinomycin D (7-AAD), respectively. The results showed that 100 μmol/L and 50 μmol/L of berberine significantly inhibited CD69 expression on T cells stimulated with PDB plus ionomycin or PHA, whereas the effect of 25 μmol/L berberine was not significant. As the incubation time increased, the extent of inhibition decreased.Similarly, the expression of CD25 was also reduced by berberine in a dose-dependent manner over the concentration range of 25-100 μmol/L. Besides, this alkaloid could block lymphocyte cell cycle progression from G0/G1 phase to S and G2/M phase without phase specificity. Moreover, analysis following 7-AAD staining revealed that berberine had no significant cytotoxicity on lymphocytes. Taken together, berberine significantly inhibits the expression of activation antigens on T lymphocytes and also blocks the progression of cell cycles of lymphocytes,suggesting that berberine may exert immunosuppressive effect through inhibiting the activation and proliferation of T cells. | Lihui Xu Yi Liu Xianhui He | 2005 | Cellular & Molecular Immunology2005，2，4： | 7 |
| 3 | Construction of Soluble Mamu-B~*1703,a Class I Major Histocompatibility Complex of Chinese Rhesus Macaques,Monomer and Tetramer Loaded with a Simian Immunodeficiency Virus Peptide显示文摘Chinese-descent rhesus macaques have become more prevalent for HIV infection and vaccine investigation than Indian-origin macaques. Most of the currently available data and reagents such as major histocompatibility complex (MHC) class I tetramers, however, were derived from Indian-origin macaques due to the dominant use of these animals in history. Although there are significant differences in the immunogenetic background between the two macaque populations, they share a few of common MHC class I alleles. We reported in this study the procedure for preparation of a soluble Mamu-B*1703 (a MHC class I molecule of Chinese macaques) monomer and tetramer loaded with a dominant simian immunodeficiency virus (SIV) epitope IW9 (IRYPKTFGW) that was identified to be Mamu-B*1701-restricted in Indian macaques. The DNA fragment encoding the Mamu-B*1703 extracellular domain fused with a BirA substrate peptide (BSP) was amplified from a previously cloned cDNA and inserted into a prokaratic expression vector. In the presence of the antigenic peptide IW9 and light chain β2-microglobulin, the expressed heavy chain was refolded into a soluble monomer. After biotinylation, four monomers were polymerized as a tetramer by phycoerythrin-conjugated streptavidin. The tetramer, having been confirmed to have the right conformation, was a potential tool for investigation of antigen-specific CD8+ T-lymphocytes in SIV vaccine models of Chinese macaques. And our results also suggested that some antigenic peptides reported in Indian-origin macaques could be directly recruited as ligands for construction of Chinese macaque MHC tetramers. | Dongyun Ouyang Xiaoying Wang Xianhui He Lihui Xu Huanjing Shi Qi Gao He Guo | 2009 | Cellular & Molecular Immunology2009，6，2： | 4 |
| 4 | Identification of major histocompatibility complex class I alleles in Chinese rhesus macaques显示文摘主要 histocompatibility 建筑群(MHC ) 一级信息为在 HIV 种痘和生物医学的模型理解有免疫力的回答的变化是重要的。在这研究, 9 Mamu -- A 和 13 Mamu-B 等位基因从 10 只中国起源的罗猴猕猴的 cDNA 产品被识别。除了被其它报导了的二等位基因,八是新奇的, 12 扩大了在 GenBank 是可得到的部分序列。MHC 一级抗原的另外的信息可能对在人的疾病研究的中国猕猴的可获得性有益。而且,领先的肽的多型性和在 α1 领域的自然漂亮受体识别主题两个都暗示那 Mamu -- A 和 Mamu-B 分子力量玩在自然漂亮房间的天生的有免疫力的回答给角色调音。 | Dongyun Ouyang Lihui Xu Zhengxi Dai Huanjing Shi Gaohong Zhang Yongtang Zheng Xianhui He | 2008 | Acta Biochimica et Biophysica Sinica2008，40，11： | 4 |
| 5 | A mini-review on ion fluxes that regulate NLRP3 inflammasome activation显示文摘The activation of NLR family pyrin domain containing 3(NLRP3)inflammasome can be induced by a wide spectrum of activators.This is unlikely achieved by the binding of different activators directly to the NLRP3 protein itself,as the activators found so far show different forms of chemical structures.Previous studies have shown that these activators can induce potassium ion(K+)and chloride ion(Cl−)efflux,calcium(Ca2+)and other ion mobilization,mitochondrial dysfunction,and lysosomal disruption,all of which are believed to cause NLRP3 inflammasome activation;how these events are induced by the activators and how they coordinate with each other in inducing the NLRP3 inflammasome activation are not fully understood.Increasing evidence suggests that the coordinated change of intracellular ion concentrations may be a common mechanism for the NLRP3 activation by different activators.In this mini-review,we present a brief summary of the current knowledge about how different ionic flows(including K+,sodium ion,Ca2+,magnesium ion,manganese ion,zinc ion,iron ion,and Cl−)are involved in regulating the NLRP3 inflammasome activation in macrophages. | Chenguang Li Mingye Chen Xianhui He Dongyun Ouyang | 2021 | Acta Biochimica et Biophysica Sinica2021，53，2： | 3 |
| 6 | Expression and Purification of Soluble Human Programmed Death-1 in Escherichia coli显示文摘规划 death-1 (PD-1 ) , CD28 家庭的一个成员,能否定地调整 TCR 由与它的血缘的 ligands (PD-L1 或 PD-L2 ) 交往的开始建筑群的发信号。 PD-1/PD-L1 小径起一个重要作用在下面调整适应有免疫力的回答的有效阶段和这条小径的封锁被证明提高抗病毒并且 antitumoral 免疫,建议让治疗的发展与病毒感染或恶意在病人改进 T 房间回答可能是一个潜在的目标。在现在的学习,有一个 carboxyl 终端他的标签的人的 PD-1 的细胞外的域(指定了为 sPD-1 ) 在 Escherichia coli 被表示为包括身体。产品是在列上 refolded,由使不能调动的金属亲密关系层析净化了,并且由西方的弄污描绘了。而且,有高纯净的可溶的 PD-1 与它的血缘的 ligand PD-L1 拥有了特定的有约束力的活动,并且分离常数是由 Scatchard 情节分析决定了的 0.43 nmol/L。这些结果建议从原核生物的房间的 refolded sPD-1 可能具有对提高抗病毒和 antitumoral 免疫者回答的治疗学的兴趣。 | Lihui Xu Yi Liu Xianhui He | 2006 | Cellular & Molecular Immunology2006，3，2： | 3 |
| 7 | Histone deacetylase inhibitor valproic acid sensitizes B16F10 melanoma cells to cucurbitacin B treatment显示文摘Cucurbitacin B (崽) 被报导与其它一起由这个代理人的联合包括黑瘤,和更有效的政体在许多肿瘤上有反增长效果在调查下面。在这研究,崽的反黑瘤效果作为一个单个代理人并且在有 valproic 酸(VPA ) 的联合, histone deacetylase (HDAC ) 的一个禁止者,在 B16F10 被评估,一根老鼠黑瘤房间线。结果证明那崽以一种剂量依赖者方式禁止了房间线的增长。然而,它是可能的支持幸存的补偿回答,包含 autophagy 的正式就职和 anti-apoptotic Bcl-2 蛋白质的 upregulation,被崽处理导致,它可能极大地减少这个代理人的 cytotoxicity。支持这,黑瘤房间被发现对有 chloroquine 的崽的联合更敏感,一个著名 autophagy 禁止者。并且导致崽的 autophagy 与 c6 月 N 终端 kinase (JNK ) 被联系激活,至少部分,自从 JNK 的抑制,由 SP600125 的活动能减轻 autophagy。当崽与 VPA 被相结合时,二药由房间 apoptosis 的正式就职显示出 synergistic cytotoxicity。而且,崽的 multiploidization 效果也面对 VPA 被压制。与由崽的 JNK 的短暂激活相对照,崽和 VPA 的联合导致了延长 JNK 激活,尽管在在 4 h 以后的底层。我们的结果证明 HDAC 禁止者 VPA 能通过 apoptotic 小径的正式就职敏化 B16F10 房间到崽处理。 | Dongyun Ouyang Yanting Zhang Lihui Xu Jingjing Li Qingbing Zha Xianhui He | 2011 | Acta Biochimica et Biophysica Sinica2011，43，6： | 3 |
| 8 | Human endogenous retroviral syncytin exerts inhibitory effect on invasive phenotype of B16F10 melanoma cells显示文摘Objective:Fusogenic endogenous retroviral syncytin plays an important role in the formation of syncytiotrophoblasts in human placenta.Apart from its expression in placenta,brain and testis,syncytin has also been found in many cancers.Although syncytin has been proposed to serve as a positive prognostic marker in some cancers,the underlying mechanism is unclear.The aim of this study is to evaluate the effects of syncytin expression on the invasive phenotype of melanoma cells.Methods:The eukaryotic expression plasmid for syncytin-EGFP was constructed and transfected into B16F10 melanoma cells.The effect of syncytin on the invasion potential of tumor cells was evaluated in B16F10 subline cells that stably expressed syncytin-EGFP fusion protein or EGFP alone.Results:The B16F10 sublines that stably expressed syncytin-EGFP or EGFP alone were established respectively and confirmed by immunofluorescent and immunoblotting assay.Syncytin expression in B16F10cells was associated with decreased cell proliferation,migration and invasion.Multinucleated giant cells that contained as many as five nuclei were induced in syncytin-expressing cells.In addition,syncytin expression did not alter the sensitivity of B16F10 cells to trichosanthin,a toxin that damages syncytiotrophoblasts more efficiently than other tissues.Conclusions:These results suggest that syncytin expression in some cancers may confine their invasion potential and thus serve as a positive prognostic factor. | Hongbo Mo Dongyun Ouyang Lihui Xu Qi Gao Xianhui He | 2013 | Chinese Journal of Cancer Research2013，25，5： | 3 |
| 9 | National Cancer Data Linkage Platform of China:Design,Methods,and Application显示文摘Background:The National Cancer Center(NCC)and China CDC cooperatively designed a National Cancer Data Linkage(NCDL)Platform to fulfill the task of sharing cancer outcome data through an automatic web-based system.Methods:NCC and China CDC established a web-based NCDL Platform to link death information from China CDC with the cancer database from NCC.Overall,76,708 cancer patients’data were analyzed to assess the feasibility and match rate of the NCDL Platform for 7 major cancers.Results:The function of the platform includes a data application and approval system,data linkage module,and results visualization system.Through the platform,38.9%cases were identified as deaths cases from the NCDL Platform in the first 3 years after cancer diagnosis.The linkage rate was highest in liver cancer and lowest in breast cancer.Conclusions:The NCDL Platform provides a powerful and efficient way to link national vital statistics with national cancer programs’data.Expanding cancer outcome data linkage may not only improve data collection efficiency,but also improve data use. | Hongmei Zeng Yunning Liu Lijun Wang Peng Yin Baohua Wang Ruiying Fu Xianhui Ran Rongshou Zheng Siwei Zhang Jiangmei Liu Jinling You Kexin Sun Shaoming Wang Li Li Ru Chen Wenqiang Wei Maigeng Zhou Jing Wu Jie He | 2022 | China CDC weekly2022，4，13： | 1 |
| 10 | Determination of ractopamine and clenbuterol in feeds by gas chromatography–mass spectrometry显示文摘 | Limin He Yijuan Su Zhenling Zeng Yahong Liu Xianhui Huang | 2006 | Animal Feed Science and Technology2006，，3： | 1 |
| 11 | The role of galU and galE of Haemophilus parasuis SC096 in serum resistance and biofilm formation显示文摘 | Yi Zou Saixiang Feng Chenggang Xu Bin Zhang Suming Zhou Lingyun Zhang Xianhui He Jingyi Li Zhen Yang Ming Liao | 2012 | Veterinary Microbiology2012，，： | 1 |
| 12 | Novel cyromazine imprinted polymer applied to the solid-phase extraction of melamine from feed and milk samples显示文摘 | Limin He Yijuan Su Yaqiu Zheng Xianhui Huang Li Wu Yahong Liu Zhenling Zeng Zhangliu Chen | 2009 | Journal of Chromatography A2009，，34： | 1 |
| 13 | The outer membrane protein P2 (OmpP2) of Haemophilus parasuis induces proinflammatory cytokine mRNA expression in porcine alveolar macrophages显示文摘 | Suming Zhou Xianhui He Chenggang Xu Bing Zhang Saixiang Feng Yi Zou Jingyi Li Ming Liao | 2013 | The Veterinary Journal2013，，： | 1 |
| 14 | Preparation and Characterization of HLA-A*0201 Tetramer Loaded with IE-1_(316-324) Antigenic Peptide of Human Cytomegalovirus显示文摘Major histocompatibility complex (MHC) class I tetramer technology has become the central technique for analyzing antigen-specific CD8+ T cell responses and it has been widely used to explore the differentiation and formation of memory CD8+ T cells. Previously, a simplified and efficient procedure for preparing high quality HLA-A*0201 tetramers has been established in our lab and the tetramers loaded with HCMV peptide pp65495-503 has been successfully applied to investigate HCMV-specific CD8+ T cells in Chinese populations. Using similar procedure we reported here the construction of HLA-A*0201 tetramer loaded with another dominant epitope derived from immediate early (IE)-1316-324 (VLEETSVML, VLE) of HCMV (A2-VLE) and characterization of this tetramer. After A2-VLE monomer was prepared and purified, its tetramer was then formed at a yield of 83%. The optimized amount of A2-VLE tetramer for staining 100 μl whole blood was 0.5 μg with incubation at 4°C for 1 h. Furthermore, the dissociation constant of the tetramer binding to the specific CD8+ T cells of one HLA-A2+ donor was estimated to be 32.7 nmol/L, which is markedly higher than that of MHC monomer. The construction of A2-VLE tetramer provides an alternative choice for investigating HCMV-specific CD8+ T cell responses and will deepen our understanding of the differentiation and formation of HCMV-specific memory CD8+ T cells. | Lihui Xu Qingbing Zha Hong Sun Qiantao Jia Fengyao Li Xianhui He | 2006 | Cellular & Molecular Immunology2006，3，5： | 1 |
| 15 | Metabolism of mequindox and its metabolites identification in chickens using LC–LTQ-Orbitrap mass spectrometry显示文摘 | Qi Shan Yiming Liu Limin He Huanzhong Ding Xianhui Huang Fan Yang Yafei Li Zhenling Zeng | 2011 | Journal of Chromatography B2011，，： | 1 |
| 16 | Cucurbitacin E suppresses cytokine expression in human Jurkat T cells through down-regulating the NF-κB signaling显示文摘Cucurbitacin E (CucE ) ,从 Cucurbitaceae 植物孤立的 triterpenoid,被显示了在 vitro 并且在 vivo 拥有 anti-inammatory 或抑制免疫力的活动,然而,内在的机制不完全地被理解。现在的学习的目的是作为一个细胞的模型在人的 Jurkat T 房间在 cytokine 表示和内在的机制上探索它的效果。结果证明 CucE 显著地禁止了 interleukin-2 的生产,肿瘤坏死因素 -- ,并且干扰素 -- 在加 ionomycin (离子) 与 phorbol 12,13-dibutyrate (PDB ) 对待的房间的文化媒介。而且, mRNA 在激活的 Jurkat T 房间这些 cytokines 铺平,建议为 cytokine 表示的批评发信号小径上的潜在的调节效果,包括原子 factor-B (NF-B ) 或激活 mitogen 的蛋白质 kinases (MAPK ) 是也在 CucE 处理之上减少了。在它表明小径的 NF-B 上的效果的支持, CucE 减少了 B (IB ) 和在 PDB + 刺激离子的房间的 NF-B/p65 的禁止者的 phosphorylation 层次。进一步支持这, NF-B/p65 的原子 translocation 显著地面对 CucE 响应 PDB 正离子刺激被压制。然而, p38MAPK, c6 月 N 终端 kinase (JNK ) ,和 Erk1/2 的 phosphorylation 没被 CucE 处理在一些时间点减少或稍微增加了。一起,这些数据建议 CucE 可以由 attenuating 展出抑制免疫力的效果批评 cytokine 表示通过下面调整发信号的 NF-B 小径。 | Lixian Wang Chenguang Li Qiuru Li Xiaoyu Zhang Hao Pan Lihui Xu Zijian Shi Dongyun Ouyang Xianhui He | 2015 | Acta Biochimica et Biophysica Sinica2015，47，6： | 1 |
| 17 | High Level Expression of HLA-A ~*0203-BSP Fusion Protein in Escherichia coli and Construction of Soluble HLA-A ~*0203 Monomer and Tetramer Loaded with Epstein-Barr Virus Peptide显示文摘Major histocompatibility complex(MHC) tetramer technology is critical for characterization of antigen-specific T cells. In the present study we reported the successful generation of HLA-A*0203 tetramer loaded with Epstein-Barr virus EBNA3596-604 peptide(SVRDRLARL,SVR) . Prokaryotic expression vector for the ectodomain of the heavy chain of HLA-A*0203 fused with a BirA substrate peptide(HLA-A*0203-BSP) was constructed and the expression conditions of the fusion protein in Escherichia coli(E. coli) were optimized. The fusion protein was highly expressed in inclusion bodies within E. coli. It was then refolded in the presence of β2-microglobulin and SVR peptide to form a soluble HLA-A*0203-SVR monomer. After biotinylation with BirA,the monomer was purified by anion-exchange chromatography and its purity was up to 95%. The tetramer was then formulated by mixing the biotinylated monomer with streptavidin-PE at a ratio of 4:1. Flow cytometry showed that this tetramer could specifically react with antigen-specific CD8+ T cells,indicating that it was biologically functional. These results provide a foundation for further characterization of antigen-specific CD8+ T cells from HLA-A*0203 subjects. | Qiantao Jia Lihui Xu Qingbing Zha Xiaoyun Chi Fengyao Li Xianhui He | 2007 | Cellular & Molecular Immunology2007，4，4： | 1 |
| 18 | Application of principal component analysis to evaluation of black soil degradation in Jilin显示文摘Principal Component Analysis(PCA) can simplify the structure of database by replacing multi-dimensional parameters with relatively less comprehensive variables in order to ensure the minimum lost in initial data.In this paper,eighteen black soil samples from different sites were tested and thirteen distinctive indexes were chosen to evaluate the degeneration of black soil.By using principal component analysis,variables of thirteen dimensions can be diminished to six unrelated principal indexes.Analysis shows that the soluble salt content,Fulvic acids(FA) and aggregation degree have a high weighing coefficient,indicating these three indexes are the major parts for the evaluation of black soil degradation.It also provides a new path to the degenerated black soil treatment in Northeast China. | NIU Cencen WANG Qing WEN Xiuyu GUO Yuan ZHAGN Penglei ZHU Rongyue HE Xianhui | 2011 | Global Geology2011，14，1： | 1 |
| 19 | Phenotypic and Functional Analysis of LCMV gp33-41-Specific CD8 T Cells Elicited by Multiple Peptide Immunization in Mice Revealed the Up-regulation of PD-1 Expression on Antigen-Specific CD8 T Cells显示文摘The phenotype and function of antigen-specific CD8 T cells are closely associated with the efficacy of a therapeutic vaccination.Here we showed that multiple immunizations with LCMV gp33-41 peptide(KAV)in Freund's adjuvant could induce KAV-specific CD8 T cells with low expression of CD127 and CD62L molecules.The inhibitory receptor PD-1 was also expressed on a substantial part of KAV-specific CD8 T cells,and its expression level on KAV-specific CD8 T cells in spleen and lymph nodes was much higher when compared to those in peripheral blood.Furthermore,KAV-specific CD8 T cells could specifically kill KAV-pulsed target cells in vivo but the efficiency was low.These data suggest that prime-boost vaccination schedule with peptide in Freund's adjuvant can elicit antigen-specific CD8 T cells of effector-like phenotype with partial functional exhaustion,which may only provide short-term protection against the pathogen. | Yi Liu Lihui Xu Yiqun Jiang Jianfang Sun Xianhui He | 2007 | Cellular & Molecular Immunology2007，4，6： | 1 |
| 20 | Co-activation for enhanced K-ion storage in battery anodes显示文摘The relative natural abundance of potassium and potentially high energy density has established potassium-ion batteries as a promising technology for future large-scale global energy storage.However,the anodes'low capacity and high dis charge platform lead to low energy density,which impedes their rapid development.Herein,we present a possible co-activation mechanism between bismuth(Bi)and tin(Sn)that enhances K-ion storage in battery anodes.The co-activated Bi-Sn anode delivered a high capacity of634 mAh g^(-1),with a discharge plateau as low as 0.35 V,and operated continuously for 500 cycles at a current density of 50 mA g^(-1),with a high Coulombic efficiency of 99.2%.This possible co-activation strategy for high potassium storage may be extended to other Na/Zn/Ca/Mg/Al ion battery technologies,thus providing insights into how to improve their energy storage ability. | Yanhong Feng Yawei Lv Hongwei Fu Mihir Parekh Apparao MRao He Wang Xiaolin Tai Xianhui Yi Yue Lin Jiang Zhou Bingan Lu | 2023 | National Science Review2023，10，7： | 0 |
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