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| 1 | Association of polymorphisms of Nramp1 gene with immune function and production performance of large white pig显示文摘The present research was designed to study the association of polymorphism of natural resistance-associated macrophage protein1 (Nramp1) with some immune function and the production performance in Large White pig. The PCR-RFLP technique was applied to analyze the correlation between the polymorphisms of Nramp1 gene and immune function [value of Polymorphonuclear Leukocytes (PMN) obtained by Nitroblue Tetrazolium (NBT) Reduction and effect of Cytotoxin in Monocyte] and production performance in 165 Large White pigs. The results showed that there was one Nde I restriction locus in Large White pig, and both values of PMN by NBT Reduction and effect of Cytotoxin in Monocyte in genotype BB were higher than those in genotype AB (P<0.05). Simultaneously, the weight of 180-day-old pigs with genotype BB was higher than that with genotype AB (P<0.05). The results indicated that there was a significant correlation between different genotypes of Nramp1 gene and Immune function and production performance, and it can be regarded as a candidate gene of disease resistance. All these results provide valuable reference to further studies of pig disease resistance. | Hongmei Wu Duxue Cheng Lixian Wang | 2008 | Journal of Genetics and Genomics2008，35，2： | 12 |
| 2 | Damage Identification in Simply Supported Bridge Based on Rotational-Angle Influence Lines Method显示文摘To locate and quantify local damage in a simply supported bridge, in this study, we derived a rotational-angle influence line equation of a simply supported beam model with local damage. Using the diagram multiplication method, we introduce an analytical formula for a novel damage-identification indicator, namely the diff erence of rotational-angle influence linescurvature(DRAIL-C). If the initial stiff ness of the simply supported beam is known, the analytical formula can be effectively used to determine the extent of damage under certain circumstances. We determined the effectiveness and anti-noise performance of this new damage-identification method using numerical examples of a simply supported beam, a simply supported hollow-slab bridge, and a simply supported truss bridge. The results show that the DRAIL-C is directly proportional to the moving concentrated load and inversely proportional to the distance between the bridge support and the concentrated load and the distance between the damaged truss girder and the angle measuring points. The DRAIL-C indicator is more sensitive to the damage in a steel-truss-bridge bottom chord than it is to the other elements. | Yu Zhou Shengkui Di Changsheng Xiang Wanrun Li Lixian Wang | 2018 | Transactions of Tianjin University2018，24，6： | 10 |
| 3 | Estimation of inbreeding and identification of regions under heavy selection based on runs of homozygosity in a Large White pig population显示文摘Background: Runs of homozygosity(ROHs) are homozygous segments of the genome where the two haplotypes inherited from the parents are identical. The current availability of genotypes for a very large number of single nucleotide polymorphisms(SNPs) is leading to more accurate characterization of ROHs in the whole genome. Here,we investigated the occurrence and distribution of ROHs in 3,692 Large White pigs and compared estimates of inbreeding coefficients calculated based on ROHs(FROH), homozygosity(FHOM), genomic relationship matrix(FGRM)and pedigree(FPED). Furthermore, we identified genomic regions with high ROH frequencies and annotated their candidate genes.Results: In total, 176,182 ROHs were identified from 3,569 animals, and all individuals displayed at least one ROH longer than 1 Mb. The ROHs identified were unevenly distributed on the autosomes. The highest and lowest coverages of Sus scrofa chromosomes(SSC) by ROH were on SSC14 and SSC13, respectively. The highest pairwise correlation among the different inbreeding coefficient estimates was 0.95 between FROH_totaland FHOM, while the lowest was-0.083 between FGRMand FPED. The correlations between FPEDand FROHusing four classes of ROH lengths ranged from 0.18 to 0.37 and increased with increasing ROH length, except for ROH > 10 Mb. Twelve ROH islands were located on four chromosomes(SSC1, 4, 6 and 14). These ROH islands harboured genes associated with reproduction, muscular development, fat deposition and adaptation, such as SIRT1, MYPN, SETDB1 and PSMD4.Conclusion: FROHcan be used to accurately assess individual inbreeding levels compared to other inbreeding coefficient estimators. In the absence of pedigree records, FROHcan provide an alternative to inbreeding estimates.Our findings can be used not only to effectively increase the response to selection by appropriately managing the rate of inbreeding and minimizing the negative effects of inbreeding depression but also to help detect genomic regions with an effect on traits under selection. | Liangyu Shi Ligang Wang Jiaxin Liu Tianyu Deng Hua Yan Longchao Zhang Xin Liu Hongmei Gao Xinhua Hou Lixian Wang Fuping Zhao | 2020 | Journal of Animal Science and Biotechnology2020，11，4： | 10 |
| 4 | Fabrication of ZnO nanorods in ionic liquids and their photoluminescent properties显示文摘A novel and simple approach is reported to fabricate uniform single-crystal ZnO nanorods in ionic liq-uids. The as-obtained ZnO nanorods have been characterized by XRD,TEM,HRTEM,SAED,XPS,EDXA,PL and UV-vis absorption spectra. The rod diameters of the nanostructures can be controlled by tuning the amount of sodium hydroxide in the synthesis. Photoluminescence results show that the nanos-tructural ZnO exhibits better optical properties than bulk ZnO does and interestingly,the smaller the rod diameters are,the better optical property 1D nanostructural ZnO exhibits. The possible growth mechanism of ZnO nanorods is also investigated. | WANG Li ZHAO Bin CHANG LiXian ZHENG WenJun | 2007 | Science China Chemistry2007，50，2： | 8 |
| 5 | Recent developments in multimodality fluorescence imaging probes显示文摘Multimodality optical imaging probes have emerged as powerful tools that improve detection sensitivity and accuracy, important in disease diagnosis and treatment. In this review, we focus on recent developments of optical fluorescence imaging(OFI) probe integration with other imaging modalities such as X-ray computed tomography(CT), magnetic resonance imaging(MRI), positron emission tomography(PET), single-photon emission computed tomography(SPECT), and photoacoustic imaging(PAI). The imaging technologies are briefly described in order to introduce the strengths and limitations of each techniques and the need for further multimodality optical imaging probe development. The emphasis of this account is placed on how design strategies are currently implemented to afford physicochemically and biologically compatible multimodality optical fluorescence imaging probes. We also present studies that overcame intrinsic disadvantages of each imaging technique by multimodality approach with improved detection sensitivity and accuracy. | Jianhong Zhao Junwei Chen Shengnan Ma Qianqian Liu Lixian Huang Xiani Chen Kaiyan Lou Wei Wang | 2018 | Acta Pharmaceutica Sinica B2018，8，3： | 6 |
| 6 | A comparative study of the characteristics of two Coxsackie A virus type 16 strains (genotype B)显示文摘Coxsackie A virus is one of the major pathogens associated with hand, foot and mouth disease (HFMD). The etiological characteristics of Coxsackie A virus type 16 (CA16) are thought to correlate with the pathological process of its infection. Two CA16 strains that were isolated from a severe HFMD patient presented with different plaque forms. This observation, along with biological analysis, indicated that the differences in the strains' biological characteristics, such as proliferation kinetics and immunogenicity, correlate with differences in their pathogenicity toward neonatal mice. Furthermore, these differences are thought to be associated with the sequence of the 5′ non-coding region of the viral genome and the VP1 structural region sequence. The results suggest that the biological and genetic characteristics of the CA16 viral strains are relevant to their pathogenicity. | YANG ErXia ZHAO Heng ZHANG Ying LIU JianSheng LIAO Yun WANG LiChun CUI PingFang YANG LiXian LIU LongDing DONG ChengHong DONG ShaoZhong SHAO CongWen JIANG Li SUN Le LI QiHan | 2012 | Science China(Life Sciences)2012，55，4： | 5 |
| 7 | Experimental optimization of phase noise performance of optoelectronic oscillator based on directly modulated laser显示文摘The experimental optimization of a 10 GHz optoelectronic oscillator (OEO) based on a 1.55 μm directly modulated distributed feedback (DFB) laser is demonstrated in this paper. The phase noise of the directly modulated laser (DML) based OEO is significantly reduced by proper selection of the laser's bias current and by using a dispersion shifted fiber as a delay line. The phase noise performance of the DML OEO achieved after optimization is close to that of a conventional OEO constructed using a commercial DFB laser and a LiNbO 3 Mach-Zehnder modulator. The DML based OEO is most promising for future realization of a miniature OEO architecture with the components integrated on a semiconductor substrate. | WANG LiXian ZHU NingHua LIU JianGuo LI Wei ZHU HongLiang WANG Wei | 2012 | Chinese Science Bulletin2012，57，31： | 4 |
| 8 | Genome-wide identification of RNA editing in seven porcine tissues by matched DNA and RNA high-throughput sequencing显示文摘Background: RNA editing is a co/posttranscriptional modification mechanism that increases the diversity of transcripts, with potential functional consequences. The advent of next-generation sequencing technologies has enabled the identification of RNA edits at unprecedented throughput and resolution. However, our knowledge of RNA editing in swine is still limited.Results: Here, we utilized RES-Scanner to identify RNA editing sites in the brain, subcutaneous fat, heart, liver,muscle, lung and ovary in three 180-day-old Large White gilts based on matched strand-specific RNA sequencing and whole-genome resequencing datasets. In total, we identified 74863 editing sites, and 92.1% of these sites caused adenosine-to-guanosine(A-to-G) conversion. Most A-to-G sites were located in noncoding regions and generally had low editing levels. In total, 151 A-to-G sites were detected in coding regions(CDS), including 94 sites that could lead to nonsynonymous amino acid changes. We provide further evidence supporting a previous observation that pig transcriptomes are highly editable at PRE-1 elements. The number of A-to-G editing sites ranged from 4155(muscle) to 25001(brain) across the seven tissues. The expression levels of the ADAR enzymes could explain some but not all of this variation across tissues. The functional analysis of the genes with tissuespecific editing sites in each tissue revealed that RNA editing might play important roles in tissue function.Specifically, more pathways showed significant enrichment in the fat and liver than in other tissues, while no pathway was enriched in the muscle.Conclusions: This study identified a total of 74863 nonredundant RNA editing sites in seven tissues and revealed the potential importance of RNA editing in tissue function. Our findings largely extend the porcine editome and enhance our understanding of RNA editing in swine. | Yuebo Zhang Longchao Zhang Jingwei Yue Xia Wei Ligang Wang Xin Liu Hongmei Gao Xinhua Hou Fuping Zhao Hua Yan Lixian Wang | 2019 | Journal of Animal Science and Biotechnology2019，10，2： | 4 |
| 9 | MATHEMATICAL MODEL OF OVERLAND FLOW AND MECHANISM OF SOIL CONSERVATION FOR FORESTED STEEP HILLSLOPE--(II)Mechanism of Soil Conservation Over Steep Hillslope显示文摘MATHEMATICALMODELOFOVERLANDFLOWANDMECHANISMOFSOILCONSERVATIONFORFORESTEDSTEEPHILLSLOPE--(II)MechanismofSoilConservationOverSt... | WU Changwen and WANG Lixian ( Science. Nanchang College of Water Resources and Hydro Power, 330029)( Dept of Soil and Water Conservation, Beijing Forestry University, 100083,PRC) | 1995 | International Journal of Sediment Research1995，10，2： | 4 |
| 10 | Sliding mode H_∞ control for a class of uncertain nonlinear state-delayed systems显示文摘A new proportional-integral (PI) sliding surface is designed for a class of uncertain nonlinear state-delayed systems. Based on this, an adaptive sliding mode controller (ASMC) is synthesized, which guarantees the occurrence of sliding mode even when the system is undergoing parameter uncertainties and external disturbance. The resulting sliding mode has the same order as the original system, so that it becomes easy to solve the H_∞ control problem by designing a memoryless H_∞ state feedback controller. A delay-dependent sufficient condition is proposed in terms of linear matrix inequalities (LMIs), which guarantees the sliding mode robust asymptotically stable and has a noise attenuation level γ in an H_∞ sense. The admissible state feedback controller can be found by solving a sequential minimization problem subject to LMI constraints by applying the cone complementary linearization method. This design scheme combines the strong robustness of the sliding mode control with the H_∞ norm performance. A numerical example is given to illustrate the effectiveness of the proposed scheme. | Wu Ligang Wang Changhong Gao Huijun Zhang Lixian | 2006 | Journal of Systems Engineering and Electronics2006，17，3： | 4 |
| 11 | Design of near-α Ti alloys via a cluster formula approach and their high-temperature oxidation resistance显示文摘The multi-component composition characteristics of high-temperature near-α Ti alloys were investigated in the present work by means of a cluster formula approach. The uniform cluster formula [CN12 cluster](glue atom)3 for the hexagonal close-packed α solid solution was first obtained based on the Friedel oscillation theory, with a total atom number in the formula of Z = 16. Then it was analyzed that the Z values in the cluster composition formulas of typical near-α Ti alloys are within the range of Z = 16.0016.30, being perfectly consistent with the ideal Z = 16. Based on it, a series of new alloys with Z = 16 and with Nb/Ta substitution for Mo in Ti1100 alloy were designed, suction-cast into φ 6 mm rods, and then heat-treated with solid solution and aging. It was found that the alloy with co-addition of Mo, Ta and Nb has a high strength and good ductility at both room and high temperatures. More importantly, the additions of Nb and Ta can contribute to the formation of continuous and compact Al2O3 scales, resulting in an obvious improvement of oxidation resistances at both 923 K and 1073 K. The effects of Mo, Ta and Nb on the oxidation behaviors of the designed alloys at 1073 K were further discussed. | Beibei Jiang Donghui Wen Qing Wang Jinda Che Chuang Dong Peter K. Liaw Fen Xu Lixian Sun | 2019 | Journal of Materials Science & Technology2019，35，6： | 4 |
| 12 | Delay-dependent stability and stabilization of a class of linear switched time-varying delay systems显示文摘The stability and stabilization of a class of linear switched time-varying delay systems are investigated. A piecewise quadratic Lyapunov function (PWQLF) is constructed and is used to obtain the stability conditions based on the linear matrix inequalities (LMIs). The stabilizing controller for this class of system is then designed and the solution of the desired controller can be obtained by a cone complementary linearization algorithm. Numerical examples are provided to illustrate the less conservativeness of the new stability and the validity of the controller design procedures. | Zhang Lixian Wang Changhong Gao Huijun | 2007 | Journal of Systems Engineering and Electronics2007，18，2： | 3 |
| 13 | The relationship between tidal current field and sediment transport in the Huanghai Sea and the Bohai Sea显示文摘-Based on a two-dimensional numerical M2 tidal model of the Huanghai and Bohai Seas, sediment transport of particles with different sizes in the computational area is given. It is concluded that tidal currents play an important role in sediment transport and deposition in the Huanghai and Bohai Seas. | Dong Lixian Su jilan and Wang Kangshan Second Institute of Oceanography. SOA, Hangzhou. China | 1989 | Acta Oceanologica Sinica1989，8，4： | 2 |
| 14 | Identification and bioinformatics analysis of lactate dehydrogenase genes from Echinococcus granulosus显示文摘Objective:To identify full length cDNA sequence of lactate dehydrogenase(LDH) from adult Echinococcus granulosus(E.granulosus) and to predict the structure and function of its encoding protein using bioinformatics methods.Methods:With the help of NCBI,EMBI, Expasy and other online sites,the open reading frame(ORF),conserved domain,physical and chemical parameters,signal peptide,epitope,topological structures of the protein sequences were predicted and a homology tertiary structure model was created:Vector NT1 software was used for sequence alignment,phylogenetic tree construction and tertiary structure prediction. Results:The target sequence was 1 233 bp length with a 996 bp biggest ORF encoding 331 amino acids protein with typical L-LDH conserved domain.It was confirmed as full length cDNA of LDH from E.granulosus and named as EgLDH(GenBank accession number:HM748917).The predicted molecular weight and isoelectric point of the deduced protein were 3 5516.2Da and 6.32 respectively.Compared with LDHs from Taenia solium,Taenia saginata asiatica,Spirometra erinaceieuropaei.Schistosoma japonicum,Clonorchis sinensis and human,it showed similarity of 86% ,85% ,55% ,58% ,58% and 53% ,respectively.EgLDH contained 3 putative transmembrane regions and 4 major epitopes(54aa-59aa.81aa-87aa,97aa-102aa,307aa-313aa),the latter were significant different from the corresponding regions of human LDH.In addition,some NAD and substrate binding sites located on epitopes 54aa-59aa and 97aa-102aa,respectively.Tertiary structure prediction showed that 3 key catalytic residues 105R,165D and 192H forming a catalytic center near the epitope 97aa-102aa,most NAD and substrate binding sites located around the center.Conclusions:The full length cDNA sequences of EgLDH were identified.It encoded a putative transmembrane protein which might be an ideal target molecule for vaccine and drugs. | Gang Lu Yajun Lu Lihua Li Lixian Wu Zhigang Fan Dazhong Shi Hu Wang Xiumin Han | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，10： | 2 |
| 15 | IgG4-related Autoimmune Pancreatitis Mimicking Acute Pancreatitis:A Case Report显示文摘IGG4-RELATED autoimmune pancreatitis (AIP) is themost common type of IgG4-related diseases. Theobservation of a diffused and localized enlargementof the pancreas and strictures in the main pancreaticduct on radiological imaging is essential fordiagnosing AIP. The clinical features of AIP which presentas a localized pancreatic mass greatly mimic pancreaticcancer. Cases with AIP mimicking pancreatic cancer arereported numerously. However, cases of IgG4-related AIPmanifesting acute pancreatitis symptoms and extensiveperipancreatic effusion are rarely observed. Meanwhile,acute pancreatitis is a common disease. If misdiagnosed asacute pancreatitis, IgG4-related AIP may progressseriously. Here, we report a rare case of IgG4-related AIPsuspected of acute pancreatitis because of epigastric painand extensive peripancreatic effusion on computed tomography(CT) images. | Xing Chen Lihua Wang Lixian Zhang Caihong Zhao | 2017 | Chinese Medical Sciences Journal2017，32，1： | 2 |
| 16 | Spatial expansion effects on urban ecosystem services supply-demand mismatching in Guanzhong Plain Urban Agglomeration of China显示文摘Global urbanization has led to drastic land use change,interfering the ecosystem services(ES)supply-demand balance,in turn threatening the well-being of humans.However,existing studies mainly stranded at the historical and current analysis,and the effects of urban spatial expansion on the relationship between ES supply and demand in the future are less clear,in particular at an urban agglomeration scale.This study was constructed with a framework of assessing the effects of urban spatial expansion on ES supply-demand mismatching under different future scenarios in the Guanzhong Plain Urban Agglomeration(GPUA)by using the Future Land Use Simulation(FLUS)model and expert-based Land-Use and Land-Cover Change(LUCC)matrix.The results showed that:(1)Urban expansion is significant in the natural development(ND)scenario,mainly manifesting the great transfer of dry land to construction land.(2)The gap between total ES supply and demand is narrowed from 2000 to 2030 and the mismatch between ES supply and demand is mainly reflected in the spatial distribution pattern in the GPUA.The ES budgets were in high surplus in Northern Qinling Mountains and northeast mountain areas,while they were in severe deficit in urban center areas.The budgets deficit under the ND scenario in 2030 is the most severe.(3)The gradient differences of ES budgets of the GPUA between urban centers and suburbs increase from 2000 to 2030 under two scenarios.The deficit region expands largest under ND scenario.The findings revealed that ES declining and supply-demand mismatching were triggered by the drastic land-use change driven by rapid urban expansion.The expansion has brought about an increasing material demand and growing industries,threatening the sustainability of ecosystems.Scenarios setting could contribute to coordinating the relationship between future urban development and ecological protection,and the policy strategies proposed in the study could inform ecological management and urban planning in the regions facing the similar urbanization situation. | PENG Lixian ZHANG Liwei LI Xupu WANG Zhuangzhuang WANG Hao JIAO Lei | 2022 | Journal of Geographical Sciences2022，32，5： | 2 |
| 17 | The landscape of chromatin accessibility in skeletal muscle during embryonic development in pigs显示文摘Background:The development of skeletal muscle in pigs during the embryonic stage is precisely regulated by transcriptional mechanisms,which depend on chromatin accessibility.However,how chromatin accessibility plays a regulatory role during embryonic skeletal muscle development in pigs has not been reported.To gain insight into the landscape of chromatin accessibility and the associated genome-wide transcriptome during embryonic muscle development,we performed ATAC-seq and RNA-seq analyses of skeletal muscle from pig embryos at 45,70 and 100 days post coitus(dpc).Results:In total,21,638,35,447 and 60,181 unique regions(or peaks)were found across the embryos at 45 dpc(LW45),70 dpc(LW70)and 100 dpc(LW100),respectively.More than 91%of the peaks were annotated within−1 kb to 100 bp of transcription start sites(TSSs).First,widespread increases in specific accessible chromatin regions(ACRs)from embryos at 45 to 100 dpc suggested that the regulatory mechanisms became increasingly complicated during embryonic development.Second,the findings from integrated ATAC-seq and RNA-seq analyses showed that not only the numbers but also the intensities of ACRs could control the expression of associated genes.Moreover,the motif screening of stage-specific ACRs revealed some transcription factors that regulate muscle developmentrelated genes,such as MyoG,Mef2c,and Mef2d.Several potential transcriptional repressors,including E2F6,OTX2 and CTCF,were identified among the genes that exhibited different regulation trends between the ATAC-seq and RNA-seq data.Conclusions:This work indicates that chromatin accessibility plays an important regulatory role in the embryonic muscle development of pigs and regulates the temporal and spatial expression patterns of key genes in muscle development by influencing the binding of transcription factors.Our results contribute to a better understanding of the regulatory dynamics of genes involved in pig embryonic skeletal muscle development. | Jingwei Yue Xinhua Hou Xin Liu Ligang Wang Hongmei Gao Fuping Zhao Lijun Shi Liangyu Shi Hua Yan Tianyu Deng Jianfei Gong Lixian Wang Longchao Zhang | 2021 | Journal of Animal Science and Biotechnology2021，12，4： | 2 |
| 18 | EGFR TKIs impair lysosome-dependent degradation of SQSTM1 to compromise the effectiveness in lung cancer显示文摘Tyrosine kinase inhibitors for epidermal growth factor receptor(EGFR TKIs)greatly improved clinical outcomes of patients with non-small cell lung cancer(NSCLC).Unfortunately,primary and acquired resistance limits their clinical benefits.To overcome such resistance,new generations of EGFR TKIs have been developed by targeting newly identified mutations in EGFR.However,much less effort has been put into alternative strategies,such as targeting the intrinsic protective responses to EGFR TKIs.In this study,we found that EGFR TKIs,including gefitinib and AZD9291,impaired lysosome-dependent degradation of SQSTM1,thus compromising their anti-cancer efficiency.By accumulating in the lysosome lumen,gefitinib and AZD9291 attenuated lysosomal acidification and impaired autolysosomal degradation of SQSTM1 owing to their intrinsic alkalinity.As a result,SQSTM1 protein was stabilized in response to gefitinib and AZD9291 treatment and conferred EGFR TKI resistance.Depleting SQSTM1 significantly increased the sensitivity of NSCLC cells to gefitinib and AZD9291 both in vitro and in vivo.Furthermore,a chemically modified gefitinib analog lacking alkalinity displayed stronger inhibitory effects on NSCLC cells.Therefore,targeting accumulated SQSTM1 or chemically modified EGFR TKIs may represent new strategies to increase the effectiveness of EGFR targeted therapy. | Lixian Yang Shilong Ying Shiman Hu Xiangtong Zhao Muchun Li Miaoqin Chen Yiran Zhu Ping Song Liyuan Zhu Tingting Jiang Huimin An Neelum Aziz Yousafzai Wenxia Xu Zhiguo Zhang Xian Wang Lifeng Feng Hongchuan Jin | 2019 | Signal Transduction and Targeted Therapy2019，4，1： | 2 |
| 19 | Generation of flat optical frequency comb using a single polarization modulator and a Brillouin-assisted power equalizer 显示文摘 | WANG Wenting SUN Wenhui WANG Lixian | 2014 | IEEE Photonies Journal(S1943-0655)2014，6，2： | 1 |
| 20 | Estimates of genomic inbreeding and identification of candidate regions that differ between Chinese indigenous sheep breeds显示文摘Background:A run of homozygosity(ROH)is a consecutive tract of homozygous genotypes in an individual that indicates it has inherited the same ancestral haplotype from both parents.Genomic inbreeding can be quantified based on ROH.Genomic regions enriched with ROH may be indicative of selection sweeps and are known as ROH islands.We carried out ROH analyses in five Chinese indigenous sheep breeds;Altay sheep(n=50 individuals),Large-tailed Han sheep(n=50),Hulun Buir sheep(n=150),Short-tailed grassland sheep(n=150),and Tibetan sheep(n=50),using genotypes from an Ovine Infinium HD SNP BeadChip.Results:A total of 18,288 ROH were identified.The average number of ROH per individual across the five sheep breeds ranged from 39(Hulun Buir sheep)to 78(Large-tailed Han sheep)and the average length of ROH ranged from 0.929 Mb(Hulun Buir sheep)to 2.544 Mb(Large-tailed Han sheep).The effective population size(Ne)of Altay sheep,Large-tailed Han sheep,Hulun Buir sheep,Short-tailed grassland sheep and Tibetan sheep were estimated to be 81,78,253,238 and 70 five generations ago.The highest ROH-based inbreeding estimate(FROH)was 0.0808 in Large-tailed Han sheep,whereas the lowest F_(ROH)was 0.0148 in Hulun Buir sheep.Furthermore,the highest proportion of long ROH fragments(>5 Mb)was observed in the Large-tailed Han sheep breed which indicated recent inbreeding.In total,49 ROH islands(the top 0.1% of the SNPs most commonly observed in ROH)were identified in the five sheep breeds.Three ROH islands were common to all the five sheep breeds,and were located on OAR2:12.2-12.3 Mb,OAR12:78.4-79.1 Mb and OAR13:53.0-53.6 Mb.Three breed-specific ROH islands were observed in Altay sheep(OAR15:3.4-3.8 Mb),Large-tailed Han sheep(ORA17:53.5-53.8 Mb)and Tibetan sheep(ORA5:19.8-20.2 Mb).Collectively,the ROH islands harbored 78 unique genes,including 19 genes that have been documented as having associations with tail types,adaptation,growth,body size,reproduction or immune response.Conclusion:Different ROH patterns were observed in five Chinese indigenous sheep breeds,which reflected their different population histories.Large-tailed Han sheep had the highest genomic inbreeding coefficients and the highest proportion of long ROH fragments indicating recent inbreeding.Candidate genes in ROH islands could be used to illustrate the genetic characteristics of these five sheep breeds.Our findings contribute to the understanding of genetic diversity and population demography,and help design and implement breeding and conservation strategies for Chinese sheep. | Jiaxin Liu Liangyu Shi Yang Li Liang Chen Dorian Garrick Lixian Wang Fuping Zhao | 2022 | Journal of Animal Science and Biotechnology2022，13，1： | 1 |
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