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| 1 | Host-Associated Quantitative Abundance Profiling Reveals the Microbial Load Variation of Root Microbiome显示文摘Plant-associated microbes are critical for plant growth and survival under natural environmental conditions.To date,most plant microbiome studies involving high-throughput amplicon sequencing have focused on the relative abundance of microbial taxa.However,this technique does not assess the total microbial load and the abundance of individual microbes relative to the amount of host plant tissues.Here,we report the development of a host-associated quantitative abundance profiling(HA-QAP)method that can accurately examine total microbial load and colonization of individual root microbiome members relative to host plants by the copy-number ratio of microbial marker gene to plant genome.We validate the HAQAP method using mock experiments,perturbation experiments,and metagenomic sequencing.The HA-QAP method eliminates the generation of spurious outputs in the classical method based on microbial relative abundance,and reveals the load of root microbiome to host plants.Using the HA-QAP method,we found that the copy-number ratios of microbial marker genes to plant genome range from 1.07 to 6.61 for bacterial 16S rRNA genes and from 0.40 to 2.26 for fungal internal transcribed spacers in the root microbiome samples from healthy rice and wheat.Furthermore,using HA-QAP we found that an increase in total microbial load represents a key feature of changes in root microbiome of rice plants exposed to drought stress and of wheat plants with root rot disease,which significantly influences patterns of differential taxa and species interaction networks.Given its accuracy and technical feasibility,HA-QAP would facilitate our understanding of genuine interactions between root microbiome and plants. | Xiaoxuan Guo Xiaoning Zhang Yuan Qin Yong-Xin Liu Jingying Zhang Na Zhang Kun Wu Baoyuan Qu Zishan He Xin Wang Xinjian Zhang Stephane Hacquard Xiangdong Fu Yang Bai | 2020 | Plant Communications2020，1，1： | 3 |
| 2 | Off-label use of recom- binant activated factor VI in intractable haemorrhage after cardio- vascular surgey: an observational study of practices in 23 Frenchcardiac centres(2005- 7) 显示文摘 | Hacquard M Durand M Lecompte T | 2011 | Eur J Cardiothorac Surg2011，40，： | 1 |
| 3 | Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analyses显示文摘 | O'Connell R J Thon M R Hacquard S et ol | 2012 | Nature Genetics2012，44，9： | 1 |
| 4 | Chasing effectors in the secretome of Melampsora larici-populina显示文摘 | DAVID L J HACQUARD S FEAU N | 2010 | Biology of Plant-microbe Interactions2010，7，： | 1 |
| 5 | Melampsora laricipopulina Transcript Profiling During Germination and Timecourse Infection of Poplar Leaves Reveals Dynamic Expression Patterns Associated with Virulence and Biotrophy显示文摘 | HACQUARD S DUPLESSIS S DELARUELLE C | 2011 | MPMI2011，24，： | 1 |
| 6 | Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome-transcriptome analyses显示文摘 | O'Connell R J Thon M R Hacquard S | 2012 | Nature Genetics2012，44，9： | 1 |
| 7 | Validation of Melampsora larici-populina reference genes for in planta RT-quantitative PCR expression profiling during time-course infection of poplar leaves显示文摘 | Stéphane Hacquard Claire Veneault-Fourrey Christine Delaruelle Pascal Frey Francis Martin Sébastien Duplessis | 2010 | Physiological and Molecular Plant Pathology2010，，3： | 1 |
| 8 | On the interaction of aspect and modal auxiliaries 显示文摘 | Hacquard V | 2009 | Linguistics and Philosophy2009，，32： | 1 |
| 9 | Lifestyle transitions in plant pathogenic Colletotrichum fungi de- ciphered by genome and transcriptome analyses显示文摘 | O'Connell R J Thon M R Hacquard S | 2012 | Na- ture Genetics2012，44，9： | 1 |
| 10 | Animal models of HLA - B27 - associated diseases: new outcomes 显示文摘 | Hacquard - Bouder C Ittah M Breban M | 2006 | Joint Bone Spine2006，73，2： | 1 |
| 11 | Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analyses 显示文摘 | OConnell R J Thon MR Hacquard S | 2012 | Nature Genetics2012，44，9： | 1 |
| 12 | Lifestyle transitions in plant pathogenic Colletotrichum fungideciphered hy genome and transcriptome analyses 显示文摘 | 0' CONNELL R J THON M R HACQUARD S | 2012 | NatGenet2012，44，9： | 1 |
| 13 | Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transeriptome analyses显示文摘 | O'Connell R J Thon M R Hacquard S | 2012 | Nature Genetics2012，44，9： | 1 |
| 14 | Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome and tran- scriptome analyses 显示文摘 | O' Connell R J Thon M R Hacquard S | 2012 | Nature Genetic2012，44，9： | 1 |
| 15 | Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analyses显示文摘 | O'Connell R J Thon M R Hacquard S | 2012 | Nature Genetics2012，44，9： | 1 |
| 16 | Lifestyle tran sitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analyses 显示文摘 | O'Connell R J Thon M R Hacquard S | 2012 | Nature Genetics2012，44，9： | 1 |
| 17 | A comprehensive analysis of genes encoding small secreted proteins identifies candidate effectors in Melampsora larici-populina (poplar leaf rust)显示文摘 | Hacquard SP Joly DL Lin YC | 2012 | Molecular Plant-Microbe Interactions2012，25，3： | 1 |
| 18 | Lifestyle transitions in plant pathogenic Colletotrichumfungi deciphered by genome and transcriptome analyses显示文摘 | O'Connell R J Thon M R Hacquard S | 2012 | Nat Genet2012，44，9： | 1 |
| 19 | Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analyses显示文摘 | O'Connell R J Thon M R Hacquard S | 2012 | Nature Genetics2012，44，9： | 1 |
| 20 | Animal models of HLA-B27-associated diseases 显示文摘 | Breban M Hacquard BC Falgarone G | 2004 | Curr Mol Med2004，4，： | 1 |
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