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| 1 | In vitro derivation of functional insulin-producing cells from human embryonic stem cells显示文摘为人的胚胎的茎(ES ) 的自强和区别的能力细胞为对待类型 Idiabetes mellitus 为胰腺的贝它细胞的产生使他们成为潜在的来源。这里,我们报导一最新发展了并且有效方法,在aserum免费的系统执行了,区分进生产胰岛素的 cells.Activin A 的导致的人的 ES 房间它在起始的阶段被使用从人的 EScells 导致权威的内胚叶区别,是由权威的内胚叶标记 Sox17 和 Brachyury.Further 的表示检测了, all-trans retinoic 酸( RA )被用来支持胰腺的区别,由早胰腺的抄写因素 pdx1 和 hlxb9 的表示显示了。在成熟 inDMEM/F12 以后有 bFGF 和菸碱的没有浆液的媒介,区分的房间表示了小岛特定的标记象 C 肽,胰岛素,胰高血糖素和 glut2 那样。百分比 ofC-peptide-positive 房间超过了 15% 。由这些房间的胰岛素和 C 肽的分泌物在葡萄糖层次对应于变化。当移植了进肾的囊时, ofStreptozotocin (STZ ) 对待裸体老鼠,这些区分的人的 ES 房间熬过并且维持贝它房间标记基因的表示包括 C 肽, pdx1, glucokinase, nkx6.1, IAPP, pax6and Tcf1。百分之三十只移植裸体老鼠展出了 stableeuglycemia 的明显的恢复;并且改正的显型被支撑超过六个星期。我们的新方法为学习人的胰开发的机制提供一个有希望的试管内区别模特儿并且说明为类型 Idiabetes mellitus 的处理使用人的 ES 房间的潜力。 | Wei Jiang Yan Shi Dongxin Zhao Song Chen Jun Yong Jing Zhang Tingting Qing Xiaoning Sun Peng Zhang Mingxiao Ding Dongsheng Li Hongkui Deng | 2007 | Cell Research2007，17，4： | 38 |
| 2 | Advanced landfill leachate treatment using a two-stage UASB-SBR system at low temperature显示文摘A two-stage upflow anaerobic sludge blanket(UASB) and sequencing batch reactor(SBR) system was introduced to treat landfill leachate for advanced removal of COD and nitrogen at low temperature.In order to improve the total nitrogen(TN) removal efficiency and to reduce the COD requirement for denitrification,the raw leachate with recycled SBR nitrification supernatant was pumped into the first-stage UASB(UASB1) to achieve simultaneous denitrification and methanogenesis.The results showed that UASB1 played an important role in COD removal and UASB2 and SBR further enhanced the nutrient removal efficiency.When the organic loading rates of UASB1,UASB2 and SBR were 11.95,1.63 and 1.29 kg COD/(m3·day),respectively,the total COD removal efficiency of the whole system reached 96.7%.The SBR acted as the real undertaker for NH4+-N removal due to aerobic nitrification.The system obtained about 99.7% of NH4+-N removal efficiency at relatively low temperature(14.9-10.9°C).More than 98.3% TN was removed through complete denitrification in UASB1 and SBR.In addition,temperature had a significant effiect on the rates of nitrification and denitrification rather than the removal of TN and NH4+-N once the complete nitrification and denitrification were achieved. | Hongwei Sun,Qing Yang,Yongzhen Peng,Xiaoning Shi,Shuying Wang,Shujun Zhang Key Laboratory of Beijing Water Quality Science and Water Envirocnnment Recovery Engineering,Beijing University of Technology,Beijing 100124,China | 2010 | Journal of Environmental Sciences2010，22，4： | 26 |
| 3 | Root microbiota shift in rice correlates with resident time in the field and developmental stage显示文摘Land plants in natural soil form intimate relationships with the diverse root bacterial microbiota. A growing body of evidence shows that these microbes are important for plant growth and health. Root microbiota composition has been widely studied in several model plants and crops; however, little is known about how root microbiota vary throughout the plant's life cycle under field conditions. We performed longitudinal dense sampling in field trials to track the time-series shift of the root microbiota from two representative rice cultivars in two separate locations in China. We found that the rice root microbiota varied dramatically during the vegetative stages and stabilized from the beginning of the reproductive stage, after which the root microbiota underwent relatively minor changes until rice ripening. Notably, both rice genotype and geographical location influenced the patterns of root microbiota shift that occurred during plant growth. The relative abundance of Deltaproteobacteria in roots significantly increased overtime throughout the entire life cycle of rice, while that of Betaproteobacteria, Firmicutes, and Gammaproteobacteria decreased. By a machine learning approach, we identified biomarker taxa and established a model to correlate root microbiota with rice resident time in the field(e.g., Nitrospira accumulated from 5 weeks/tillering in field-grown rice). Our work provides insights into the process of rice root microbiota establishment. | Jingying Zhang Na Zhang Yong-Xin Liu Xiaoning Zhang Bin Hu Yuan Qin Haoran Xu Hui Wang Xiaoxuan Guo Jingmei Qian Wei Wang Pengfan Zhang Tao Jin Chengcai Chu Yang Bai | 2018 | Science China(Life Sciences)2018，61，6： | 24 |
| 4 | DNA methyltransferases and methyl-binding proteins of mammals显示文摘在哺乳动物, DNA methylation,从 S-adenosylmethionines 由甲基组的转移描绘了到一个底(主要指了 cytosine ) ,充当主要 epigenetic 修正。在到 DNA 序列安排的平行,到 DNA 序列的 methylation 的修正在生物功能和活动上有影响深远的影响因为它涉及控制基因抄写,调整染色质结构,支撑染色体稳定性并且正直,维持父母印和X染色体 inactivation ,象限制 transposable 元素一样压制相应再结合,在哪个期间 DNA methyltransferases ( DNMT )和甲基绑定蛋白质玩重要 rol 他们的离开正路能产生基因表示的 dysregulation,房间 maltransformation 等等。因此,获得蛋白质的这二种重要类型的好理解是必要的,它将帮助更好调查 epigenetic 机制并且基于它的可逆性根据我们的愿望操作修正。这里,我们简短在哺乳动物考察我们 DNMT 和甲基绑定蛋白质的当前的理解。 | Jie Lan Song Hua Xiaoning He Yong Zhang | 2010 | Acta Biochimica et Biophysica Sinica2010，42，4： | 20 |
| 5 | Blockade of Tim-3 Pathway Ameliorates Interferon-γ Production from Hepatic CD8^+ T Cells in a Mouse Model of Hepatitis B Virus Infection显示文摘T cell immunoglobulin-and mucin-domain-containing molecule-3(Tim-3) has been reported to participate in the pathogenesis of inflammatory diseases. However,whether Tim-3 is involved in hepatitis B virus(HBV) infection remains unknown. Here,we studied the expression and function of Tim-3 in a hydrodynamics-based mouse model of HBV infection. A significant increase of Tim-3 expression on hepatic T lymphocytes,especially on CD8+ T cells,was demonstrated in HBV model mice from day 7 to day 18. After Tim-3 knockdown by specific shRNAs,significantly increased IFN-γ production from hepatic CD8+ T cells in HBV model mice was observed. Very interestingly,we found Tim-3 expression on CD8+ T cells was higher in HBV model mice with higher serum anti-HBs production. Moreover,Tim-3 knockdown influenced anti-HBs production in vivo. Collectively,our data suggested that Tim-3 might act as a potent regulator of antiviral T-cell responses in HBV infection. | Ying Ju Nan Hou Xiaoning Zhang Di Zhao Ying Liu Jinjin Wang Fang Luan Wei Shi Faliang Zhu Wensheng Sun Lining Zhang Chengjiang Gao Lifen Gao Xiaohong Liang Chunhong Ma | 2009 | Cellular & Molecular Immunology2009，6，1： | 19 |
| 6 | Immunotherapy using IL-2 and GM-CSF is a potential treatment for multidrug-resistant Mycobacterium tuberculosis显示文摘This study investigated the therapeutic effects of interleukin (IL)-2 and granulocyte-macrophage colony-stimulating factor (GM-CSF) coadministrated with antibacterial agents isoniazid (INH) and rifampin (RIF) to treat a mouse model of tuberculosis (TB) infection. A drug-susceptible TB strain, H37Rv was used to infect mice and the effectiveness of IL-2 and GM-CSF was initially evaluated based on survival rate, bacterial counts in lungs and spleens and the pathological condition of the lungs. Next, the therapeutic effect of the immunotherapy regimen was assessed in multidrug-resistant strain OB35-infected mice. In the H37Rv infection model, IL-2 and GM-CSF monotherapies reduced bacterial numbers in the lungs by 0.82 (P<0.01) and 0.58 (P<0.05) lg colony-forming units (CFU), respectively, and in the spleens by 1.42 (P<0.01) and 1.22 (P<0.01) lg CFU, respectively, compared with the untreated group. Mice receiving immunotherapy developed fewer lesions in the lungs compared with mice receiving antibacterial therapy alone. In the OB35 infection model, immunotherapy with either cytokine resulted in a significant reduction of bacterial load in the lungs and spleens and less severe lesions in the lungs compared with the untreated or antibacterial therapy treated mice. Notably, mice receiving immunotherapy with both cytokines had a 30% survival rate which was higher than that in other treated groups, and had significantly less CFUs in the lungs and spleens (1.02 and 1.34 lg CFU) compared with antibacterial therapy alone (P<0.01). This study demonstrated that immunotherapy with both IL-2 and GM-CSF may be useful to treat multidrug resistant tuberculosis (MDR-TB). | ZHANG YongRong LIU Jian WANG Yong XIAN Qiaoyang SHAO LingYun YANG Zhong WANG XiaoNing | 2012 | Science China(Life Sciences)2012，55，9： | 15 |
| 7 | Relationship between the -455G/A and -148C/T polymorphisms in the beta-fibrinogen gene and cerebral infarction in the Xinjiang Uygur and Han Chinese populations显示文摘We sought to investigate the correlation between the -455G/A and -148C/T polymorphisms of the β-fibrinogen gene and plasma fibrinogen levels in patients with cerebral infarction and in healthy subjects among the Xinjiang Uygur and Han Chinese populations, by using polymerase chain reaction-restriction enzyme digestion analysis. Results showed that there were no statistically significant differences in the distributions of the -455G/A genotype and allele frequency between the Uygurs and the Han. Plasma fibrinogen levels in cerebral infarction patients among the Uygurs and the Han were higher than those among healthy subjects. In particular, the frequencies of the -455G/A AA and -148C/T TT genotypes were significantly higher than in healthy subjects. Individuals carrying the A or T allele had a higher incidence of cerebral infarction compared with those carrying the G or C allele. Our experimental findings indicate that the -148C/T and -455G/A polymorphisms are associated with cerebral infarction in Xinjiang Uygur and Han Chinese subjects. The susceptibility- conferring alleles are -148T and -455A, and the susceptibility-conferring genotype is -455G/A + AA. | Xiaoning Zhang Yanyun Li Xuebing Guo Lei Du Jianhua Ma | 2012 | Neural Regeneration Research2012，7，7： | 13 |
| 8 | Rock fragmentation mechanisms and an experimental study of drilling tools during high-frequency harmonic vibration显示文摘Resonance drilling is a new technology,still at the laboratory stage.It has great potential to improve rock fragmentation efficiency.We analyzed the amplitude-frequency characteristics of steady-state mechanical vibration excited by harmonic vibration in rocks and an apparatus was built to achieve high frequency vibration of rock.The influence of rock drillability,rotary speed,excitation frequency,and other parameters on the rate of penetration(ROP) in resonance drilling was analyzed.The results show that the rock drillability decreased with an increase in excitation frequency.When drilling with a large size drill bit,the ROP increased with excitation frequency.The ROP reached a maximum value at the resonant frequency of the rock.The ROP of the bit increased linearly with rotary speed when no vibration was applied on the rock and increased approximately exponentially when harmonic vibration was applied.In addition,the resonant frequency of the rock was changing during the process of rock fragmentation,so in order to achieve the desired resonance of the rock,it is necessary to determine an appropriate harmonic vibration excitation frequency. | Li Wei Yan Tie Li Siqi Zhang Xiaoning | 2013 | Petroleum Science2013，10，2： | 9 |
| 9 | Aurora A orchestrates entosis by regulating a dynamic MCAK-TIP150 interaction显示文摘 | PengXia JinhuaZhou Xiaoyu Song BingWu XingLiu Di Li Shuyuan Zhang Zhikai Wang H uijuan Yu Tarsha Ward Jiancun Zhang Yinmei Li Xiaoning Wang YongChen Zhen Guo Xuebiao Yao | 2014 | Journal of Molecular Cell Biology2014，8，3： | 9 |
| 10 | Effect of diosmetin on airway remodeling in a murine model of chronic asthma显示文摘支气管的气喘,最普通的过敏疾病之一,被航线 hyperresponsiveness (AHR ) 描绘,发炎,并且改变。抗氧化剂黄酮 aglycone diosmetin 改善在胰腺炎的发炎,而是很少在气喘上对它的影响被知道。在这研究,长期的气喘上的 diosmetin 的效果在航线在与 ovalbumin (卵) 质问的 BALB/c 老鼠改变的调整上与一个重音被调查。diosmetin 显著地在气喘的老鼠的肺减轻了煽动性的细胞渗入,离脚玻璃杯或瓷杯细胞增生,和骨胶原免职并且显著地在这些动物减少了 AHR,这被发现。全部的房间的导致卵的增加和在 bronchoalveolar lavage 液体的嗜曙红血球计数被颠倒,并且在浆液的卵特定的免疫球蛋白 E 的水平被 diosmetin 管理稀释,暗示 diosmetin 的一项 anti-Th2 活动。而且, diosmetin 显著地压制了光滑的肌肉肌动朊 alpha 链的表示,显示航线上的 diosmetin 的有势力 anti-proliferative 效果光滑的肌肉房间(ASMC ) 。矩阵 metallopeptidase-9,转变生长 factor-1,和脉管的 endothelial 生长因素层次是也由 diosmetin 减轻了,建议航线改变的宽恕可能被归因于这些蛋白质的衰落。一起拿,我们的调查结果向 diosmetin 的新奇侧面提供了改变反的治疗学的好处,在在长期的气喘汇寄 ASMC 增长加亮 diosmetin 的一个新潜力。 | Ai Ge Yanan Liu Xiaoning Zeng Hui Kong Yuan Ma Jiaxiang Zhang Fangfang Bai Mao Huang | 2015 | Acta Biochimica et Biophysica Sinica2015，47，8： | 7 |
| 11 | IL-17RD (Sef or IL-17RLM) interacts with IL-17 receptor and mediates IL-17 signaling显示文摘Interleukin-17 (IL-17 或 IL-17A ) 生产是 TH17 房间的一个特点,贡献多重自体免疫、煽动性的疾病的致病的 CD4+ T 淋巴细胞的一个新唯一的系。IL-17 受体(IL-17R 或 IL-17RA ) 为 IL-17 生物活动是必要的。新兴的数据建议 heteromeric 或 homomeric 受体建筑群的形成为 IL-17 发信号被要求。这里,我们证明孤儿受体 IL-17RD (Sef,类似的表示到 FGF 基因或 IL-17RLM ) 被联系并且有 IL-17R 的 colocalized。重要地, IL-17RD 调停 IL-17 发信号,用一个酶记者评估了由 24p3 的本国的倡导者开车, IL-17 目标基因。另外,主导否定地缺乏细胞内部的领域的 IL-17RD 异种压制 IL-17R-mediated IL-17 发信号。而且,象 IL-17R 一样的 IL-17RD 与 TRAF6 被联系,一个 IL-17R 下游的分子。这些结果显示 IL-17RD 是表明建筑群的 IL-17 受体的部分,因此为通过 heteromeric 或 homomeric 受体建筑群发信号的 IL-17 提供新奇证据。 | Zhili Rong Anan Wang Zhiyong Li Yongming Ren Long Cheng Yinghua Li Yinyin Wang Fangli Ren Xiaoning Zhang Jim Hu Zhijie Chang | 2009 | Cell Research2009，19，2： | 6 |
| 12 | A novel xeno-free and feeder-cell-free system for human pluripotent stem cell culture显示文摘While human induced pluripotent stem cells(hiPSCs)have promising applications in regenerative medicine,most of the hiPSC lines available today are not suitable for clinical applications due to contamination with nonhuman materials,such as sialic acid,and potential pathogens from animal-product-containing cell culture systems.Although several xeno-free cell culture systems have been established recently,their use of human fibroblasts as feeders reduces the clinical potential of hiPSCs due to batch-to-batch variation in the feeders and time-consuming preparation processes.In this study,we have developed a xeno-free and feeder-cell-free human embryonic stem cell(hESC)/hiPSC culture system using human plasma and human placenta extracts.The system maintains the self-renewing capacity and pluripotency of hESCs for more than 40 passages.Human iPSCs were also derived from human dermal fibroblasts using this culture system by overexpressing three transcription factors—Oct4,Sox2 and Nanog.The culture system developed here is inexpensive and suitable for large scale production. | Qihui Wang Xiaoning Mou Henghua Cao Qingzhang Meng Yanni Ma Pengcheng Han Junjie Jiang Hao Zhang Yue Ma | 2012 | Protein & Cell2012，3，1： | 6 |
| 13 | EGb761抑制β-淀粉样蛋白_(25-35)片段诱导的神经元凋亡显示文摘以 β-淀粉样蛋白2 5 35片段 (β-AmyloidPeptide 2 5 35,β AP2 5 35)诱发原代培养小鼠皮层神经元出现凋亡作为阿尔采末症 (Alzheimer′sDisease ,AD)的离体模型 ,在此基础上观察了银杏叶提取物 (ExtractofleavesofGinkgobilobaL .,EGb76 1)及其主要活性成分内酯B(GinkgolideB ,GinB)对上述神经元损伤的对抗作用 .结果表明 ,EGb76 1能抑制 β AP2 5 35引起的神经元活性的下降和超微结构的改变 ,即抑制 β AP2 5 35诱导的神经元凋亡 ,从而为EGb76 1临床用于防治AD提供了实验依据 . | Gao Jing Zhu Li Zhao Xiaoning Zhang Zuxuan | 2001 | 南京大学学报(自然科学版)2001，37，2： | 6 |
| 14 | Role of Microphysical Parameterizations with Droplet Relative Dispersion in IAP AGCM 4.1显示文摘Previous studies have shown that accurate descriptions of the cloud droplet effective radius(Re) and the autoconversion process of cloud droplets to raindrops(Ar) can effectively improve simulated clouds and surface precipitation, and reduce the uncertainty of aerosol indirect effects in GCMs. In this paper, we implement cloud microphysical schemes including two-moment Ar and Re considering relative dispersion of the cloud droplet size distribution into version 4.1 of the Institute of Atmospheric Physics' s atmospheric GCM(IAP AGCM 4.1), which is the atmospheric component of the Chinese Academy of Sciences' Earth System Model. Analysis of the effects of different schemes shows that the newly implemented schemes can improve both the simulated shortwave and longwave cloud radiative forcings, as compared to the standard scheme, in IAP AGCM 4.1. The new schemes also effectively enhance the large-scale precipitation, especially over low latitudes, although the influences of total precipitation are insignificant for different schemes. Further studies show that similar results can be found with the Community Atmosphere Model, version 5.1. | Xiaoning XIE He ZHANG Xiaodong LIU Yiran PENG Yangang LIU | 2018 | Advances in Atmospheric Sciences2018，35，2： | 6 |
| 15 | A survey of autoencoder-based recommender systems显示文摘In the past decade,recommender systems have been widely used to provide users with personalized products and services.However,most traditional recommender systems are still facing a challenge in dealing with the huge volume,complexity,and dynamics of information.To tackle this challenge,many studies have been conducted to improve recommender system by integrating deep learning techniques.As an unsupervised deep learning method,autoencoder has been widely used for its excellent performance in data dimensionality reduction,feature extraction,and data reconstruction.Meanwhile,recent researches have shown the high efficiency of autoencoder in information retrieval and recommendation tasks.Applying autoencoder on recommender systems would improve the quality of recommendations due to its better understanding of users,demands and characteristics of items.This paper reviews the recent researches on autoencoder-based recommender systems.The differences between autoencoder-based recommender systems and traditional recommender systems are presented in this paper.At last,some potential research directions of autoencoder-based recommender systems are discussed. | Guijuan ZHANG Yang LIU Xiaoning JIN | 2020 | Frontiers of Computer Science2020，14，2： | 6 |
| 16 | Improved MOEA/D for Dynamic Weapon-Target Assignment Problem显示文摘Conducting reasonable weapon-target assignment( WTA) with near real time can bring the maximum awards with minimum costs which are especially significant in the modern war. A framework of dynamic WTA( DWTA) model based on a series of staged static WTA( SWTA) models is established where dynamic factors including time window of target and time window of weapon are considered in the staged SWTA model. Then,a hybrid algorithm for the staged SWTA named Decomposition-Based Dynamic Weapon-target Assignment( DDWTA) is proposed which is based on the framework of multi-objective evolutionary algorithm based on decomposition( MOEA / D) with two major improvements: one is the coding based on constraint of resource to generate the feasible solutions, and the other is the tabu search strategy to speed up the convergence.Comparative experiments prove that the proposed algorithm is capable of obtaining a well-converged and well diversified set of solutions on a problem instance and meets the time demand in the battlefield environment. | Ying Zhang Rennong Yang Jialiang Zuo Xiaoning Jing | 2015 | Journal of Harbin Institute of Technology(New Series)2015，22，6： | 6 |
| 17 | A real-time forecast of tunnel fire based on numerical database and artificial intelligence显示文摘The extreme temperature induced by fire and hot toxic smokes in tunnels threaten the trapped personnel and firefighters.To alleviate the potential casualties,fast while reasonable decisions should be made for rescuing,based on the timely prediction of fire development in tunnels.This paper targets to achieve a real-time prediction(within 1 s)of the spatial-temporal temperature distribution inside the numerical tunnel model by using artificial intelligence(Al)methods.A CFD database of 100 simulated tunnel fire scenarios under various fire location,fire size,and ventilation condition is established.The proposed Al model combines a Long Short-term Memory(LSTM)model and a Transpose Convolution Neural Network(TCNN).The real-time ceiling temperature profile and thousands of temperature-field images are used as the training input and output.Results show that the predicted temperature field 60 s in advance achieves a high accuracy of around 97%.Also,the Al model can quickly identify the critical temperature field for safe evacuation(i.e.,a critical event)and guide emergency responses and firefighting activities.This study demonstrates the promising prospects of Al-based fire forecasts and smart firefighting in tunnel spaces. | Xiqiang Wu Xiaoning Zhang Xinyan Huang Fu Xiao Asif Usmani | 2022 | Building Simulation2022，15，4： | 6 |
| 18 | Theoretical and experimental study of the pulling force of jet bits in radial drilling technology显示文摘Radial drilling technology,of which the jet bit is the key device,is a research focus in the field of oil drilling and production.This paper establishes mechanical equations for jet bits and analyzes the hydroseal of backward jets in bottom holes.Meanwhile this paper establishes a mechanical equation for a high pressure hose and analyzes the axial force distribution.Laboratory experiments indicate that the flow rate,the angle between the backward nozzle axis and the jet bit axis,and the hole diameter are the major influencing factors;the generation of the pulling force is mainly due to the inlet pressure of the jet bit;the backward jets can significantly increase not only the pulling force but also the stability of jet bits.The pulling force would reach 8,376 N under experimental conditions,which can steadily pull the high-pressure hose forward. | Ruichang Guo Gensheng Li Zhongwei Huang Shouceng Tian Xiaoning Zhang Wei Wu | 2009 | Petroleum Science2009，6，4： | 5 |
| 19 | Integrating full-length transcriptomics and metabolomics reveals the regulatory mechanisms underlying yellow pigmentation in tree peony(Paeonia suffruticosa Andr.)flowers显示文摘Tree peony(Paeonia suffruticosa Andr.)is a popular ornamental plant in China due to its showy and colorful flowers.However,yellow-colored flowers are rare in both wild species and domesticated cultivars.The molecular mechanisms underlying yellow pigmentation remain poorly understood.Here,petal tissues of two tree peony cultivars,“High Noon”(yellow flowers)and“Roufurong”(purple–red flowers),were sampled at five developmental stages(S1–S5)from early flower buds to full blooms.Five petal color indices(brightness,redness,yellowness,chroma,and hue angle)and the contents of ten different flavonoids were determined.Compared to“Roufurong,”which accumulated abundant anthocyanins at S3–S5,the yellow-colored“High Noon”displayed relatively higher contents of tetrahydroxychalcone(THC),flavones,and flavonols but no anthocyanin production.The contents of THC,flavones,and flavonols in“High Noon”peaked at S3 and dropped gradually as the flower bloomed,consistent with the color index patterns.Furthermore,RNA-seq analyses at S3 showed that structural genes such as PsC4Hs,PsDFRs,and PsUFGTs in the flavonoid biosynthesis pathway were downregulated in“High Noon,”whereas most PsFLSs,PsF3Hs,and PsF3’Hs were upregulated.Five transcription factor(TF)genes related to flavonoid biosynthesis were also upregulated in“High Noon.”One of these TFs,PsMYB111,was overexpressed in tobacco,which led to increased flavonols but decreased anthocyanins.Dual-luciferase assays further confirmed that PsMYB111 upregulated PsFLS.These results improve our understanding of yellow pigmentation in tree peony and provide a guide for future molecular-assisted breeding experiments in tree peony with novel flower colors. | Xiaoning Luo Daoyang Sun Shu Wang Sha Luo Yaqi Fu Lixin Niu Qianqian Shi Yanlong Zhang | 2021 | Horticulture Research2021，8，1： | 5 |
| 20 | Natural orifice transluminal endoscopic surgery in urology: The Chinese experience显示文摘Objective:To describe the Chinese experience of natural orifice transluminal endoscopic surgery(NOTES)in urology.Methods:From December 2008 to May 2017,35 animal experiments and 305 clinical surgeries of NOTES or natural orifices specimen extractions(NOSE)were performed in China.The animal experiments included five kidney biopsies,24 nephrectomies and six partial nephrectomies.The clinical surgeries included 12 transvaginal NOSE(TV-NOSE),266 hybrid transvaginal NOTES(TV-NOTES)and 27 pure TV-NOTES.The TV-NOSE procedure was performed in five transumbilical laparoendoscopic single-site(U-LESS)nephrectomies,four suprapubic-assisted laparoendoscopic single-site surgery(SA-LESS)nephroureterectomies,and three laparoscopic radical cystectomies.The hybrid TV-NOTES procedure included 210 nephrectomies,31 adrenalectomies,eight nephroureterectomies,13 partial nephrectomies,and four heminephrectomies.The pure TV-NOTES procedure included five renal cyst decortications and 22 nephrectomies.Results:A total of 29 animal experiments were successfully performed.One partial nephrectomy was converted to standard laparoscopic surgery.Two kidney biopsies and two nephrectomies were unsuccessful.A total of 297 clinical surgeries were successfully performed.Six patients who underwent hybrid TV-NOTES were converted to open surgery.Two patients who underwent pure TV-NOTES were converted to SA-LESS.There were 22 major complications,16 occurred intraoperatively and six postoperatively.The mean visual analog score(VAS)of 48 h after the operation was 2.5 points in TV-NOSE,2.3 points in hybrid TV-NOTES and 1.7 points in pure TV-NOTES.The mean follow-up of 50.6(3.0-87.0)months showed that all patients were in good condition.The umbilicus scars were nearly invisible in TV-NOSE and hybrid TV-NOTES.The vaginal incision healed well.Conclusions:TV-NOSE and TV-NOTES are feasible,safe,and effective with little injury,low pain,fast recovery,and good cosmetic outcomes in properly selected patients.They are worth consideration for urological clinical practice. | Xiaofeng Zou Guoxi Zhang Tianpeng Xie Yuanhu Yuan Rihai Xiao Gengqing Wu Xiaoning Wang Hui Xu Folin Liu Yuting Wu Yunfeng Liao Quanliang Liu Yinghao Sun Bo Yang Linhui Wang Chuanliang Xu Xiaofeng Gao | 2020 | Asian Journal of Urology2020，7，1： | 5 |
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