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| 1 | Measurement of total phenolic content and antioxidant activity of aerial parts of medicinal plant Coronopus didymus显示文摘Objective: To evaluate the total phenolic content and compare the antioxidant activity of various solvent extracts and fractions from the aerial parts of Coronopus didymus through various assays. Methods: Total phenolic content was determined using the Folin-Ciocalteu assay and the in vitro antioxidant activity of a number of different extracts was investigated in a dose-dependent manner with three different methods: the 2,2-diphenyl-1-picrylhydrazyl(DPPH), 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt(ABTS) and ferric reducing antioxidant power(FRAP) assays. A flavone was isolated from the most active ethanolic extract with high antioxidant activity using size exclusion chromatography. IC_(50) values were calculated for the DPPH and ABTS methods. The FRAP activity was assessed in terms of μM Fe(II) equivalent. Results: The phenolic content was found to be highest in the ethanol extract(CDA Et; 47.8 mM GAE) and the lowest in the dichloromethane extract(CDA DCM; 3.13 mM GAE). The ethanol extract showed high radical scavenging activity towards DPPH and ABTS radicals with IC_(50) values of(7.80 × 10~2) and(4.32 × 10~2) μg/m L, respectively. The most active ethanol extract had a FRAP value of 1 921.7 μM Fe(Ⅱ) equivalent. The isolated flavone F10C(5,7,4'-trihydroxy-3'-methoxy flavone) was far more effective for scavenging free radicals in the DPPH and ABTS assays with IC_(50) of 43.8 and 0.08 μg/m L, than the standard trolox, with IC_(50) values of 97.5 and 21.1 μg/m L, respectively. In addition, the flavone F10C and the standard ascorbic acid had FRAP values of 1 621.7 and 16 038.0 μM Fe(Ⅱ) equivalents, respectively. Conclusions: The total phenolic content of extracts in decreasing order is ethanol extract(CDA Et)> acetone extract(CDA ACE)> phenolic extract(CDA MW)> n-hexane extract(CDA nHX)> chloroform extract(CDA CHL)> dichloromethane extract(CDA DCM). The ordering of extracts in terms of antioxidant activity from highest to lowest is CDA Et> CDA MW> CDA DCM> CDA CHL> CDA ACE> CDA nHX in DPPH, ABTS and FRAP assays. A significant relationship is found between antioxidant potential and total phenolic content, suggesting that phenolic compounds are the major contributors to the antioxidant activity of Coronopus didymus. | Hafiza Noreen Nabil Semmar Muhammad Farman James S.O.McCullagh | 2017 | Asian Pacific Journal of Tropical Medicine2017，10，8： | 9 |
| 2 | Interaction between diet composition and gut microbiota and its impact on gastrointestinal tract health显示文摘A substantial amount of emerging research is indicating that the gut microbiota has a significant impact on human health.Alterations of gut microbiota have clear consequences on intestinal homeostasis,physiology,gut microbiome,immune system and host metabolic pathways.Diet composition plays an important role in the control of gut microbial populations and,thus,in the prevention,management and treatment of certain diseases such as cancer,diabetes.A comprehensive analysis of previously reported results revealed that the gut microbiota can be modulated by diet and the composition of gut microbiota can be influenced by various diet components.The symbiotic relationship between different gut microbial communities regulates the immune system and,therefore,any dysbiosis can dysregulate the immune system.Further research is needed to fully understand the mechanisms involved in the interactions between diet composition,gut microbiota and associated diseases. | Muhammad Shahid Riaz Rajoka Junling Shi Hafiza Mahreen Mehwish Jing Zhu Qi Li Dongyan Shao Qingsheng Huang Hui Yang | 2017 | Food Science and Human Wellness2017，6，3： | 7 |
| 3 | Factors affecting delivery and transient expression of gusA gene in Malaysian indica rice MR 219callus via biolistic gun system显示文摘 | Zuraida AR Rahiniza K Hafiza MRN | | 0，，51： | 1 |
| 4 | Influence of overlap between the laser beam tracks on surface quality in laser polishing of AISI H13 tool steell 显示文摘 | Hafiza A M K Vbordatchevb E Tutunea F R O | 2012 | Journal of Manufacturing Processes2012，14，4： | 1 |
| 5 | Development of honey hydrogel dressing for enhanced wound healing显示文摘 | YUSOF N HAFIZA A H A ZOHDI R M | 2007 | Radiation Physics and Chemistry2007，76，： | 1 |
| 6 | Identification of reentry circuit sites during catheter mapping and radiofrequency ablation of ventricular tachycardia late after myocardial infarction显示文摘 | Stevenson WG Hafiza K Sager P | 1993 | Circulation1993，88，： | 1 |
| 7 | Non-small cell lung carcinoma(NSCLC):Implications on molecular pathology and advances in early diagnostics and therapeutics显示文摘Continuous revision of the histologic and stage-wise classification of lung cancer by the World Health Organization (WHO) provides the foundation for therapeutic advances by promoting molecular targeted and immunotherapies and ensuring accurate diagnosis. Cancer epidemiologic data provide helpful information for cancer prevention, diagnosis, and management, supporting health-care interventions. Global cancer mortality projections from 2016 to 2060 show that cancer will overtake ischemic heart diseases (IHD) as the leading cause of death (18.9 million) immediately after 2030, surpassing non-small cell lung cancer (NSCLC), which accounts for 85 percent of lung cancers. The clinical stage at the diagnosis is the main prognostic factor in NSCLC therapies. Advanced early diagnostic methods are essential as the initial stages of cancer show reduced mortality compared to the advanced stages. Sophisticated approaches to proper histological classification and NSCLC management have improved clinical efficiency. Although immune checkpoint inhibitors (ICIs) and targeted molecular therapies have refined the therapeutic management of late-stage NSCLC, the specificity and sensitivity of cancer biomarkers should be improved by focusing on prospective studies, followed by their use as therapeutic tools. The liquid biopsy candidates such as circulating tumor cells (CTCs), circulating cell-free tumor DNA (cfDNA), tumor educated platelets (TEP), and extracellular vesicles (EVs) possess cancer-derived biomolecules and aid in tracing: driver mutations leading to cancer, acquired resistance caused by various generations of therapeutic agents, refractory disease, prognosis, and surveillance. | Hafiza Padinharayil Jinsu Varghese Mithun Chacko John Golgodu Krishnamurthy Rajanikant Cornelia M.Wilson Minnatallah Al-Yozbaki Kaviyarasi Renu Saikat Dewanjee Rupa Sanyal Abhijit Dey Anirban Goutam Mukherjee Uddesh Ramesh Wanjari Abilash Valsala Gopalakrishnan Alex George | 2023 | Genes & Diseases2023，10，3： | 1 |
| 8 | Heavy metal contents in soil of major towns in the east coast of Peninsular Malaysia显示文摘 | Seng Chee Poh Norhaxati Mohd Tahir Hafiza Mohamed Zuki Mohd Izwadi Musa Khai Hock Ng Noor Azhar Mohamed Shazili | 2006 | Chinese Journal Of Geochemistry2006，25，B08： | 1 |
| 9 | Association of pro12ala polymorphism in peroxisome proliferator activated receptor gamma with proliferative diabetic retinopathy显示文摘 | Tariq Khadija Malik Saira Bano Ali Syeda Hafiza Benish | 2013 | Mol Vis2013，19，： | 1 |
| 10 | Effect of Radiofrequency Catheter Ablation of Ventricular Tachycardia on Left Ventricular Function in Patients with Prior Myocardial Infarction显示文摘 | Hafiza H. Khan William H. Maisel Carolyn Ho Makoto Suzuki Kyoko Soejima Scott Solomon William G. Stevenson | 2002 | Journal of Interventional Cardiac Electrophysiology2002，，3： | 1 |
| 11 | Socio-economic background of wastewater irrigation and bioaccumulation of heavy metals in crops and vegetables显示文摘 | Shameem Raja Hafiza Masooma Naseer Cheema Shaista Babar | 2015 | Agricultural Water Management2015，158，： | 1 |
| 12 | Predicting adolescent posttraumatic stress in the aftermath of war: Differential effects of coping strategies across trauma reminder, loss reminder, and family conflict domains显示文摘 | Kathryn H. Howell Julie B. Kaplow Christopher M. Layne Molly A. Benson Bruce E. Compas Ranka Katalinski Hafiza Pasalic Nina Bosankic Robert Pynoos | 2015 | Anxiety, Stress, & Coping2015，，1： | 1 |
| 13 | Extraction of DNA suitable for PCR applications from mature leaves of Mangifera indica L.显示文摘Good quality deoxyribonucleic acid (DNA) is the pre-requisite for its downstream applications. The presence of high concentrations of polysaccharides, polyphenols, proteins, and other secondary me- tabolites in mango leaves poses problem in getting good quality DNA fit for polymerase chain reaction (PCR) applications. The problem is exacerbated when DNA is extracted from mature mango leaves. A reliable and modified protocol based on the cetyl- trimethylammonium bromide (CTAB) method for DNA extraction from mature mango leaves is described here. High concentrations of inert salt were used to remove polysaccharides; Polyvinylpyrrolidone (PVP) and β-mercaptoethanol were employed to manage phenolic compounds. Extended chloroform-isoamyl alcohol treatment followed by RNase treatment yielded 950?1050 μg of good quality DNA, free of protein and RNA. The problems of DNA degradation,contamination, and low yield due to irreversible binding of phenolic compounds and coprecipitation of polysaccharides with DNA were avoided by this method. The DNA isolated by the modified method showed good PCR amplification using simple se- quence repeat (SSR) primers. This modified protocol can also be used to extract DNA from other woody plants having similar problems. | Muhammad Abubakkar AZMAT Iqrar Ahmad KHAN Hafiza Masooma Naseer CHEEMA Ishtiaq Ahmad RAJWANA Ahmad Sattar KHAN Asif Ali KHAN | 2012 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2012，13，4： | 1 |
| 14 | Glinus lotoides ethanolic extract alleviates LPS-induced anxiety and depression-like behavior by modulating antioxidant and inflammatory biomarkers in rats显示文摘Objective:To explore the protective role of Glinus lotoides ethanolic extract in a depression model through modulating oxidant/antioxidant enzyme system and inflammatory status.Methods:Phytochemical constituents of Glinus lotoides ethanolic extract were evaluated qualitatively and quantitatively along with HPLC.Rats were divided into six groups.The normal control and the intoxicated groups received normal saline,and the standard group received imipramine,while the remaining groups received 100,300,and 500 mg/kg Glinus lotoides ethanolic extract.All groups received treatments for 14 d.Lipopolysaccharides(LPS)were then administered i.p.(0.83 mg/kg)to all groups except the normal control group.After 24 h,anxiety and depression-like behaviors were evaluated by performing behavioral analysis(open field,tail suspension,forced swim,sucrose preference test),and determining total oxidant status,total antioxidant capacity,catalase,and biochemical parameters[malondialdehyde,glutathione,superoxide dismutase,tumor necrosis factor(TNF)-alpha and interleukin(IL)-6].Results:Phytochemical studies confirmed the presence of phenols and flavonoids and HPLC analysis showed the presence of gallic acid,quercetin,chlorogenic,and caffeic acid.Total oxidant status was significantly decreased,while total antioxidant capacity was significantly increased in the Glinus lotoides ethanolic extract treated groups.Moreover,Glinus lotoides ethanolic extract diminished malondialdehyde,IL-6,and TNF-alpha levels,while increasing superoxide dismutase,catalase,and glutathione activities.Conclusions:Glinus lotoides ethanolic crude extract shows significant antidepressant activity by modulating oxidative and biochemical parameters that supports its folkloric use in traditional systems of medicine. | Ambreen Mehmood Awan Wafa Majeed Faraza Javed Bilal Aslam Asra Iftikhar Hafiza Arooj Kanwal Sobia Fiaz | 2022 | Asian Pacific Journal of Tropical Biomedicine2022，12，2： | 1 |
| 15 | Comparison of vaginal misoprostol and dinoprostone for cervical ripening before diagnostic hysteroscopy in nulliparous women 显示文摘 | Hasan Ali I Zeynep Hafiza OI Esra T | 2015 | Fertility & Sterility2015，103，5： | 1 |
| 16 | Green5G: Enhancing Capacity and Coverage in Device-to-Device Communication显示文摘With the popularity of green computing and the huge usage of networks,there is an acute need for expansion of the 5G network.5G is used where energy efficiency is the highest priority,and it can play a pinnacle role in helping every industry to hit sustainability.While in the 5G network,conventional performance guides,such as network capacity and coverage are still major issues and need improvements.Device to Device communication(D2D)communication technology plays an important role to improve the capacity and coverage of 5G technology using different techniques.The issue of energy utilization in the IoT based system is a significant exploration center.Energy optimizationin D2D communication is an important point.We need to resolve this issue for increasing system performance.Green IoT speaks to the issue of lessening energy utilization of IoT gadgets which accomplishes a supportable climate for IoT systems.In this paper,we improve the capacity and coverage of 5G technology using Multiple Inputs Multiple Outputs(MU-MIMO).MUMIMO increases the capacity of 5G in D2D communication.We also present all the problems faced by 5G technology and proposed architecture to enhance system performance. | Abdul Rehman Javed Rabia Abid Bakhtawar Aslam Hafiza Ammara Khalid Mohammad Zubair Khan Omar H.Alhazmi Muhammad Rizwan | 2021 | Computers, Materials & Continua2021，，5： | 0 |
| 17 | 球孢白僵菌对柑橘木虱内共生菌的影响显示文摘为明确球孢白僵菌[Beauveria bassiana(Balsamo)]侵染柑橘木虱后其体内共生菌的变化,研究虫生真菌、柑橘木虱及内共生菌三者间的互作关系,采用荧光原位杂交技术(FISH),测定2个球孢白僵菌(FAFU-12和FAFU-16)菌株亚致死浓度(LC_(25))对柑橘木虱F_(1)代内共生菌Wolbachia和Carsonella的影响。结果表明,与对照相比,FAFU-12和FAFU-16处理组中柑橘木虱F1代2种内共生菌红色荧光分布范围减少,荧光强度减弱。球孢白僵菌亚致死剂量处理改变了柑橘木虱F_(1)代内共生菌Wolbachia和Carsonella的含量及分布,2种内共生菌参与了虫生真菌侵染宿主致病过程中的互作。 | 戴佳旺 王会停 崔施施 方元 徐宏涛 赖文昶 杨晨 李小芳 李新安 HAFIZA Javaria Ashraf 王联德 | 2021 | 武夷科学2021，37，2： | 0 |
| 18 | Expression profiling of transgenes(Cry1Ac and Cry2A) in cotton genotypes under different genetic backgrounds显示文摘Transgenic cotton carrying the CrylAc gene has revolutionized insect pest control since its adoption,although the development of resistance in insect pests has reduced its efficacy.After 10 years of cultivating Bacillus thuringiensis(Bt)cotton with a single Cry1 Ac gene,growers are on the verge of adopting Bt cotton that carries the double gene(Cry1 Ac+Cry2 A)due to its better effectiveness against insect pests.Thus,the current study was designed to evaluate the role of each gene in the effectiveness of Bt cotton carrying the double gene.The expression levels of the Cry1 Ac and Cry2 A genes were evaluated in the leaves of 10 genotypes(2 parents and 8 Fhybrids)at 30 days after sowing(DAS),while samples of leaves,bolls and flowers were taken from the upper and lower canopies at 70 and 110 DAS.The Fhybrids were developed through reciprocal crosses between two Bt(CKC-1,CKC-2)and two non-Bt(MNH-786,FH-942)parents.The differential expression of transgenes was evaluated through Enzyme Linked Immuno-Sorbent Assay(ELISA).The results showed that the MNH786 xCKC-1 hybrid had the highest concentrations of Cry1 Ac gene at30 DAS(3.08μg g^(-1))and 110 DAS(1.01μg g^(-1))in leaves.In contrast,the CKC-2 xMNH-786 hybrid showed the lowest concentrations of Cry1 Ac gene at 30 DAS(2.30μg g^(-1))and 110 DAS(0.86μg g^(-1)).The Fhybrid FH-942×CKC-2 showed the highest concentrations of Cry2 A gene at 30 DAS(8.39μg g^(-1))and 110 DAS(7.74μg g^(-1))in leaves,while the CKC-1 xMNH-786 hybrid expressed the lowest concentrations of Cry2 A gene at 30 DAS(7.10μg g^(-1))and 110 DAS(8.31μg g^(-1)).A comparison between the two stages of plant growth showed that leaves had the highest concentrations at 30 DAS,whereas the lowest concentrations were observed at 110 DAS for both genes in leaves.When the expression pattern was compared between various plant parts in genotype CKC-2,it was found that leaves had higher concentrations of Cry1 Ac(3.12μg g^(-1))and Cry2 A(8.31μg g^(-1))at 70 DAS,followed by bolls(Cry1 Ac(1.66μg g^(-1))and Cry2 A(8.15μg g^(-1)))and flowers(Cry1 Ac(1.07μg g^(-1))and Cry2 A(7.99μg g^(-1))).The genotype CKC-2 had higher concentrations of Cry1 Ac(3.12μg g^(-1))and Cry2 A(8.31μg g^(-1))in the upper canopy but less accumulation(2.66μg g^(-1)of Cry1 Ac,8.09μg g^(-1)of Cry2 A)in the lower canopy at 70 DAS.Similarly,at 110 DAS,the expression levels of Cry1 Ac and Cry2 A in upper and lower canopy leaves were 1.52 and 7.92μg 9,and 0.99 and 7.54μg 9,respectively.Hence,the current study demonstrates that different genotypes showed variable expression for both of the Cry1 Ac and Cry2 A genes during plant growth due to different genetic backgrounds.The Cry2 A gene had three-fold higher expression than Cry1 Ac with significant differences in expression in different plant parts.The findings of this study will be helpful for breeding insect-resistant double-gene genotypes with better gene expression levels of Cry1 Ac and Cry2 A for sustainable cotton production worldwide. | Kashif NOOR Hafiza Masooma Naseer CHEEMA Asif Ali KHAN Rao Sohail Ahmad KHAN | 2022 | Journal of Integrative Agriculture2022，21，10： | 0 |
| 19 | Identification and characterization of plasma membrane aquaporins isolated from fiber cells of Calotropis procera显示文摘Calotropis procera, commonly known as 'milkweed', possesses long seed trichomes for seed dispersal and has the ability to survive under harsh conditions such as drought and salinity. Aquaporins are water channel proteins expressed in all land plants, divided into five subfamilies plasma membrane intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs), NOD26-like proteins (NIPs), small basic intrinsic proteins (SIPs), and the unfamiliar X intrinsic proteins (XIPs). PIPs constitute the largest group of water channel proteins that are involved in different developmental and regulatory mechanisms including water permeability, cell elongation, and stomata opening. Aquaporins are also involved in abiotic stress tolerance and cell expansion mechanisms, but their role in seed trichomes (fiber cells) has never been investigated. A large number of clones isolated from C. procera fiber cDNA library showed sequence homology to PIPs. Both expressed sequence tags (ESTs) and real-time polymerase chain reaction (PCR) studies revealed that the transcript abundance of this gene family in fiber cells of C. procera is greater than that of cotton. Full-length cDNAs of CpPIP1 and CpPIP2 were isolated from C. procera fiber cDNA library and used for constructing plant expression vectors under constitutive (2×35S) and trichome-specific (GhLTP3) promoters. Transgenic tobacco plants were developed via Agrobacterium-mediated transformation. The phenotypic characteristics of the plants were observed after confirming the integration of transgene in plants. It was observed that CpPIP2 expression cassette under 2×35S and GhLTP3 promoter enhanced the numbers of stem and leave trichomes. However, 2×35S::CpPIP2 has a more amplified effect on trichome density and length than GhLTP3::CpPIP2 and other PIP constructs. These findings imply the role of C. procera PIP aquaporins in fiber cell elongation. The PIPs-derived cell expansion mechanism may be exploited through transgenic approaches for improvement of fiber staple length in cotton and boosting of defense against sucking insects by enhancing plant pubescence. | Usman ASLAM Asia KHATOON Hafiza Masooma Naseer CHEEMA Aftab BASHIR | 2013 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2013，14，7： | 0 |
| 20 | Numerical Treatment for Stochastic Computer Virus Model显示文摘This writing is an attempt to explain a reliable numerical treatment for stochastic computer virus model.We are comparing the solutions of stochastic and deterministic computer virus models.This paper reveals that a stochastic computer virus paradigm is pragmatic in contrast to the deterministic computer virus model.Outcomes of threshold number C^?hold in stochastic computer virus model.If C^?<1 then in such a condition virus controlled in the computer population while C^?>1 shows virus persists in the computer population.Unfortunately,stochastic numerical methods fail to cope with large step sizes of time.The suggested structure of the stochastic non-standard finite difference scheme(SNSFD)maintains all diverse characteristics such as dynamical consistency,boundedness and positivity as defined by Mickens.The numerical treatment for the stochastic computer virus model manifested that increasing the antivirus ability ultimates small virus dominance in a computer community. | Ali Raza Muhammad Shoaib Arif Muhammad Rafiq Mairaj Bibi Muhammad Naveed Muhammad Usman Iqbal Zubair Butt Hafiza Anum Naseem Javeria Nawaz Abbasi | 2019 | Computer Modeling in Engineering & Sciences2019，，8： | 0 |
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