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| 1 | Effect of combined medicated thread moxibustion plus needle picking therapy of Zhuang nationality medicine on antioxidant levels in a rat model of sciatica显示文摘OBJECTIVE: To investigate the effect and underlying mechanisms of combined medicated thread moxibustion therapy plus needle picking therapy of Zhuang nationality medicine on antioxidant levels in a rat model of sciatica.METHODS: One hundred Wistar rats, of specific pathogen free level, were randomly divided into five groups: normal control group, model group,medicated thread moxibustion group, needle picking group, and combination group. Each group contained 20 rats. In the model, medicated thread moxibustion, needle picking, and combination groups,sciatica models were established through chronicconstriction injury of the sciatic nerve. After the model was established, the rats in the medicated thread moxibustion, needle picking, and combination groups were given the corresponding therapies for 21 days. The control and model groups received no treatment. Reactive oxygen species, superoxide dismutase, malondialdehyde, and total antioxidant capacity changes were determined.Nicotinamide adenine dinucleotide phosphate(NADPH) oxidase subunit NADPH oxidases 4(NOX4) m RNA expression and the morphology of cells were observed to detect apoptosis of gamma-aminobutyric acid ergic(GABAergic) neurons.RESULTS: Compared with control group, reactive oxygen species and malondialdehyde levels rose significantly in the model group(P < 0.01), while superoxide dismutase and total antioxidant capacity levels were lowered(P < 0.05). Compared with the model group, reactive oxygen species and malondialdehyde decreased in the needle picking group(P < 0.05), while superoxide dismutase levels were increased(P < 0.05); reactive oxygen species and malondialdehyde significantly decreased in the combination group(P < 0.01). In addition, the model group had higher NOX4 m RNA expression than that of the control group(P < 0.05), and the combination group had lower expression levels than that of the model group(P < 0.05). Apoptosis of GABAergic neurons was observed in the model group, and was attenuated after combined therapy.CONCLUSION: The medicated thread moxibustion therapy plus needle picking therapy of Zhuang nationality medicine can prevent oxidative damage in the rat model of sciatica via down-regulating NOX4 expression, improving antioxidant capacity, and in-hibiting the oxidative damage pathway of GABAergic neurons. | Tang Hanqing Zheng Jianyu Li Tianzi Li Keming Li Xiaohua Dou Xibin Huang Chunchuan Liang Ye | 2016 | Journal of Traditional Chinese Medicine2016，36，6： | 4 |
| 2 | Chloroplast genomic diversity in Bulbophyllum section Macrocaulia(Orchidaceae,Epidendroideae,Malaxideae):Insights into species divergence and adaptive evolution显示文摘Bulbophyllum is the largest genus in Orchidaceae with a pan tropical distribution.Due to highly significant diversifications,it is considered to be one of the most taxonomically and phylogenetically complex taxa.The diversification pattern and evolutionary adaptation of chloroplast genomes are poorly understood in this species-rich genus,and suitable molecular markers are necessary for species determination and phylogenetic analysis.A natural Asian section Macrocaulia was selected to estimate the interspecific divergence of chloroplast genomes in this study.Here,we sequenced the complete chloroplast genome of four Bulbophyllum species,including three species from section Macrocaulia.The four chloroplast genomes had a typical quadripartite structure with a genome size ranged from 156,182 to 158,524 bp.The chloroplast genomes included 113 unique genes encoding 79 proteins,30 tRNAs and 4 rRNAs.Comparison of the four chloroplast genomes showed that the three species from section Macrocaulia had similar structure and gene contents,and shared a number of indels,which mainly contribute to its monophyly.In addition,interspecific divergence level was also great.Several exclusive indels and polymorphism SSR loci might be used for taxonomical identification and determining interspecific polymorphisms.A total of 20 intergenic regions and three coding genes of the most variable hotspot regions were proposed as candidate effective molecular markers for future phylogenetic relationships at different taxonomical levels and species divergence in Bulbophyllum.All of chloroplast genes in four Bulbophyllum species were under purifying selection,while 13 sites within six genes exhibited sitespecific selection.A whole chloroplast genome phylogenetic analysis based on Maximum Likelihood,Bayesian and Parsimony methods all supported the monophyly of section Macrocaulia and the genus of Bulbophyllum.Our findings provide valuable molecular markers to use in accurately identifying species,clarifying taxonomy,and resolving the phylogeny and evolution of the genus Bulbophyllum.The molecular markers developed in this study will also contribute to further research of conservation of Bulbophyllum species. | Hanqing Tang Lu Tang Shicheng Shao Yulan Peng Lu Li Yan Luo | 2021 | Plant Diversity2021，43，5： | 3 |
| 3 | A new robust circular Gabor based object matching by using weighted Hausdorff distance显示文摘 | Zhu Zhenfeng Tang Ming Lu Hanqing | 2004 | Pattern Recognition Letters2004，25，4： | 1 |
| 4 | Hydrophilic interaction uhra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry for highly rapid and sensitive analysis of underivatized amino acids in functional foods 显示文摘 | Zhou Guisheng Pang Hanqing Tang Yuping | 2013 | Amino Acids2013，，44： | 1 |
| 5 | A New Robust Circular Gabor Based Object Matching by Using Weighted Hausdorff Distance 显示文摘 | Zhu Zhenfeng Tang Ming Lu Hanqing | 2004 | Pattern Recognition Letters2004，25，4： | 1 |
| 6 | In vivo Implantation of Hydrophobic Acrylic Intraocular Lenses with Surface Modification显示文摘Purpose:To assess the surface properties of modified hydrophobic acrylic intraocular lenses (IOL) implanted in rabbits.Methods:.The hydrophobic acrylic IOLs were modified with monomer vinyl pyrrolidone by surface modification technique.Phacoemulsification combined with IOL implantation was conducted in 9 rabbits (18 eyes).Postoperative responses were observed by slit-lamp microscope at 3,.7,.15,.30,.90 days after surgery.Results:.During the early stage after IOL implantation,corneal edema and anterior chamber fibrin exudation were observed.The exudate fluid was almost absorbed at the 15th day postoperatively.At 7th day,the anterior chamber exudation in the modification group was significantly less severe than that in non-modification group(P<0.05).Posterior capsular opacification occurred at 30th day after surgery and was aggravated90 days later.IOL dislocation was seen in 5 eyes and occlusion of pupil in 3 eyes.Conclusion:The hydrophobic acrylic IOLs with surface modification have improved surface properties and higher uveal biocompatibility. | Guiqin Wang Liqun Cao Na Li Xiujun Peng Huiqin Tang Rongxin Wan Hanqing Gu | 2013 | Eye Science2013，28，4： | 0 |
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