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| 1 | New insights into estrogenic regulation of O^6-methylguanine DNA-methyltransferase (MGMT) in human breast cancer cells: Co-degradation of ER-α and MGMT proteins by fulvestrant or O^6-benzylguanine indicates fresh avenues for therapy显示文摘Endocrine therapy using estrogen receptor-α(ER-α) antagonists for attenuating horm2one-driven cell proliferation is a major treatment modality for breast cancers.To exploit any DNA repair deficiencies associated with endocrine therapy,we investigated the functional and physical interactions of ER-α with O^6-methylguanine DNA methyltransferase(MGMT),a unique DNA repair protein that confers tumor resistance to various anticancer alkylating agents.The ER-α-positive breast cancer cell lines(MCF-7,T47D) and ER- negative cell lines(MDAMB-468,MDAMB-231),and established inhibitors of ER-α and MGMT,namely,ICI-182,780(Faslodex) and O^6-benzylguanine,respectively,were used to study MGMT- ER interactions.The MGMT gene promoter was found to harbor one full and two half estrogen-responsive elements(EREs) and two antioxidant-responsive elements(AREs).MGMT expression was upregulated by estrogen,downregulated by tamoxifen in Western blot and promoter-linked reporter assays.Similarly,both transient and stable transfections of Nrf-2(nuclear factor-erythroid 2-related factor-2)increased the levels of MGMT protein and activity 3 to 4-fold reflecting novel regulatory nodes for this drugresistance determinant.Of the different ER-α antagonists tested,the pure anti-estrogen fulvestrant was most potent in inhibiting the MGMT activity in a dose,time and ER-α dependent manner,similar to O^6-benzylguanine.Interestingly,fulvestrant exposure led to a degradation of both ER-α and MGMT proteins and O^6-benzylguanine also induced a specific loss of ER-α and MGMT proteins in MCF-7 and T47 D breast cancer cells with similar kinetics.Immunoprecipitation revealed a specific association of ER-α and MGMT proteins in breast cancer cells.Furthermore,silencing of MGMT gene expression triggered a decrease in the levels of both MGMT and ER-α proteins.The involvement of proteasome in the drug-induced degradation of both proteins was also demonstrated.Fulvestrant enhanced the cytotoxicity of MGMT-targeted alkylating agents,namely,temozolomide and BCNU by 3 to 4-fold in ER-α positive cells,but not in ER-negative cells.We conclude that MGMT and ER-α proteins exist as a complex and are co-targeted for ubiquitin-conjugation and subsequent proteasomal degradation.The findings offer a clear rationale for combining alkylating agents with endocrine therapy. | Ameya Paranjpe Nathan I. Bailes Santhi Konduri George C. Bobustuc Francis Ali-Osman Mohd. A. Yusuf Surendra R. Punganuru Hanumantha Rao Madal Debasish Basak AGM Mostofa Kalkunte S. Srivenugopa | 2016 | The Journal of Biomedical Research2016，30，5： | 5 |
| 2 | Leptosririllumferrooxidans-sulfide mineral interactions with reference to bioflotation nad bioflocculation显示文摘铁的离子的粘附黄铁矿和黄铜矿矿物质上的成年 Leptospirillum ferrooxidans 房间通过吸附,希腊语的第六个字母潜力和弥漫的反射英尺红外被调查大小。当絮凝行为被 Turbiscan 大小检验时,矿物质 floatability 上的细菌的种类的影响被 Hallimond 筹款测试决定。矿物质等电位的点(pH 6.5 ? 7.5 ) 在和细菌的房间的相互作用向房间转移了等电位的点以后(pH 3.3 ) ,在矿物质上显示房间吸附的化学性质出现。与细菌的房间对待的矿物质的英尺红外系列显示出所有房间的存在功能的组意味着房间吸附。黄铜矿上的细菌的房间吸附与黄铁矿相比是更高的,它同意了房间与黄铁矿比较的黄铜矿筹款和显著絮凝行为上的更大的萧条效果。 | A. VILINSKA K. HANUMANTHA RAO | 2008 | 中国有色金属学会会刊:英文版2008，18，6： | 5 |
| 3 | In vitro antioxidant and in vivo anti-inflammatory potential of crude polysaccharide from Turbinaria ornata (Marine Brown Alga)显示文摘 | Subash Ananthi Hanumantha Rao Balaji Raghavendran Adoor Gopalan Sunil Veeraraghavan Gayathri Ganapathy Ramakrishnan Hannah R. Vasanthi | 2009 | Food and Chemical Toxicology2009，，1： | 1 |
| 4 | Antifertility efficacy of the plant Striga lutea (Scrophulariacae) on rats 显示文摘 | Hiremath SP Hanumantha RS | 1990 | Contraception1990，42，4： | 1 |
| 5 | Development of hybrid model and optimization of surface roughness in electric discharge machining using artificial neural networks and genetic algorithm 显示文摘 | Krishna Mohana Rao G Rangajanardhaa G Hanumantha Rao D | 2009 | Journal of Materials Processing Technology2009，209，3： | 1 |
| 6 | Adhesion of Paenibacillus polymyxa on chalcopyrite and pyrite:surface thermodynamics and extended DLVO theory 显示文摘 | Sharma P K Hanumantha R K | 2003 | Colloid and Surfaces Biointerfaces2003，29，1： | 1 |
| 7 | Surface characterization of Acidithiobacillus ferrooxidans cells grown under different conditions显示文摘 | Sharma P K Dasb A Hanumantha R K | 2003 | Hydrometallurgy2003，71，3： | 1 |
| 8 | Surface characterization of Acidithiobacillus ferrooxidans cells grown under different conditions显示文摘 | Sharma P K Das A Hanumantha Rao K | 2003 | Hydrometallurgy2003，71，8： | 1 |
| 9 | Immunomodulatory activity of fucoidan against aspirin-induced gastric mucosal damage in rats显示文摘 | Hanumantha Rao Balaji Raghavendran Periasamy Srinivasan Sathyanath Rekha | 2010 | International Immunopharmacology2010，，2： | 1 |
| 10 | Atomistic simulation of the surface structure of wollastonite and adsorption phenomena relevant to flotation显示文摘 | Kundu T K Hanumantha K Parker S C | 2003 | International Journal of Mineral Processing2003，72，： | 1 |
| 11 | Adhesion of paeniba-cillus polymyxa on chalcopyrite and pyrite: surface thermo dynamics and extended DLVO theory 显示文摘 | Sharma P K Hanumantha R K | 2003 | Colloids and Surfaces B: Biointerfaces2003，29，1： | 1 |
| 12 | Adhesion of paenibacillus polymyxa on chalcopyrite and pyrite:Surface thermodynamics and extended DLVO theory显示文摘 | Sharma P K Hanumantha R K | 2003 | Colloids and Surfaces B:Biointerfaces2003，29，1： | 1 |
| 13 | Analysis of different approaches for evaluation of surface energy of microbial cells by contact angle goniometry显示文摘 | Sharma P Hanumantha Rao K | 2002 | Advances in Colloid and Interface Science2002，98，3： | 1 |
| 14 | Adsorption mechanism of mixed cationic/anionic collectors in feldspar-quartz flotation system显示文摘 | A. Vidyadhar K. Hanumantha Rao | 2006 | Journal of Colloid And Interface Science2006，，2： | 1 |
| 15 | Fine coal preparation using novel tri- bo-electrostatic separator显示文摘 | Dwari R K Hanumantha R K | 2009 | Minerals Engineering2009，22，2： | 1 |
| 16 | Analysis of different approaches for evaluation of surface energy of microbial ceils by contact angle goni- ometry显示文摘 | Sharma p k Hanumantha Rao K | 2002 | Advances in Colloid and Interface Science2002，，： | 1 |
| 17 | Mixed collector systems in flotation显示文摘 | K. Hanumantha Rao K.S.E. Forssberg | 1997 | International Journal of Mineral Processing1997，，1： | 1 |
| 18 | Adhesion of Paenibacillus polymyxa on chalcopyrite and pyrite:Surface thermodynamics and extended DLVO theory显示文摘 | Sharma P K Hanumantha R K | 2003 | Colloids and Surfaces B:Biointerfaces2003，29，1： | 1 |
| 19 | Mechanisnls of amine-feldspar interaction in the absence and presence of alcohols studied by spectroscopic method 显示文摘 | Vidyadhar A Hanumantha Rao K Chemyshova I V | 2002 | Journal of Colloid and Interface Science2002，256，1： | 1 |
| 20 | Adhesion of Paenibacillus polymyxa on chalcopyrite and pyrite: Surface thermodynamics and extended DLVO theory 显示文摘 | SHARMA P K HANUMANTHA R K | 2003 | Collids and Surfaces B: Biointerfaces2003，29，10： | 1 |
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