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| 1 | 益生菌和酸奶对乳糖不耐受者结肠菌群作用研究显示文摘目的研究补充益生菌和酸奶对乳糖不耐者结肠菌群的影响。方法以经25g乳糖负荷试验筛选的乳糖不耐受者11名为对象,让受试者持续摄入益生菌(含B.longum)和酸奶(含B.animalis,L.bulgaricus,S.thermophilus)14天进行补充研究。试验分3阶段:即补充前阶段7天,补充阶段14天,补充后阶段7天。在补充阶段开始前1天和结束后1天分别进行乳糖不耐受负荷试验,记录受试者不耐受症状并评分。在试验阶段收集受试者的新鲜粪便样本共5次,采用荧光原位杂交技术(FISH)和变性梯度凝胶电泳技术(DGGE)检测补充前、补充期间和补充后粪便样本细菌总数和各主要菌群的数量构成,以及外源性和内源性双歧杆菌菌种分布。结果在补充益生菌胶囊和酸奶后受试者乳糖不耐受症状评分均显著降低(P<0·05)。补充期间细菌总数和主要细菌组(Bifidobacteria,Bacteroides-Prevotella,Eubacteria,Ruminccocc等)的数量均显著高于补充前(P<0·05),双歧杆菌的数量在补充结束后7天仍显著高于补充前(P<0·05),其他几组细菌数量在补充结束后下降;DGGE结果显示来自益生菌胶囊和酸奶的双歧杆菌菌种在补充期间能够在受试者肠道内检出,但个体间存在较大差异。结论益生菌胶囊和酸奶中的双歧杆菌能够耐受胃肠的酸碱环境在结肠内存活和繁殖,并影响内源性双歧杆菌和其他细菌组的数量,改变结肠菌群的构成,尤其以双歧杆菌更为明显,这对减轻乳糖不耐受症状起到重要作用。 | 钟燕 黄承钰 何涛 Harmsen HMJ Vonk RJ | 2006 | 卫生研究2006，35，5： | 11 |
| 2 | Metformin does not improve survival in patients with hepatocellular carcinoma显示文摘AIM:To assess whether metformin,which has a chemopreventive effect in chronic liver disease,has any chemotherapeutic effect in hepatocellular carcinoma.METHODS:This was a retrospective study of 701 patients with newly diagnosed hepatocellular carcinoma(HCC)seen between January 2005 and June 2011 at Mayo Clinic,Rochester,Minnesota.This patient cohort was a part of the global HCC BRIDGE study,which is a large longitudinal study of HCC determining the realworld experience of HCC characteristics,management and patient outcomes.We defined significant metformin exposure as continuation of this agent at least 90d beyond diagnosis of HCC,and compared survival of diabetic patients on metformin to diabetic patients not on metformin and non-diabetics.RESULTS:Our cohort was 72.9%male,with a mean±SD age of 62.6±12.3 years.The most common etiologies of liver disease were hepatitis C(34%),alcoholic liver disease(29%),fatty liver disease(15%)and hepatitis B(9%).By univariate analysis,using diabetics not on metformin as the reference group,diabetic patients with HCC on metformin had no survival advantage,with a HR(95%CI)of 1.0(0.8-1.3).Non-diabetic HCC patients also did not appear to have a survival advantage as compared to diabetic HCC patients not on metformin,as demonstrated by a HR(95%CI)of1.1(0.7-1.7).Diabetics on metformin beyond 90 d after HCC diagnosis had a longer median survival at 34.2 mo,as compared to 25.5 mo among diabetic patients who were not on metformin or had discontinued metformin within 90 d after HCC diagnosis.This finding was likely due to potential survival bias among those who lived long enough to receive metformin.CONCLUSION:Although the literature suggests a chemotherapeutic effect in other malignancies,our study demonstrates no survival benefit to the use of metformin in diabetic patients with HCC. | Mamatha Bhat Roongruedee Chaiteerakij William S Harmsen Cathy D Schleck Ju Dong Yang Nasra H Giama Terry M Therneau Gregory J Gores Lewis R Roberts | 2014 | World Journal of Gastroenterology2014，20，42： | 11 |
| 3 | 应用荧光原位杂交技术分析乳糖不耐受者结肠菌群显示文摘目的 : 探讨结肠菌群构成与乳糖不耐受 (lactose intolerance,LI)症状之间的关系 ,以及饮奶对结肠双歧杆菌数量的影响。方法 : 根据乳糖负荷试验 ,结合问卷调查筛选成人乳糖吸收不良者、轻度及重度乳糖不耐受者作为试验对象。收集受试者一次新鲜全便 ,选用 5种特异性的 1 6S r RNA寡核苷酸探针 ,应用荧光原位杂交技术检测结肠细菌总数以及几种主要菌属 (种 )的构成。结果 : 乳糖不耐受组与吸收不良组相比 ,细菌总数和双歧杆菌数有显著性差异 (P<0 .0 5) ,细菌总数与乳糖不耐受症状评分之间呈负相关 (P<0 .0 5) ;每次饮奶以平均 2 0 0 ml计 5~ 7次 / w饮奶组与 0~ 2次 / w饮奶组或 0次 / w饮奶组相比 ,双歧杆菌数量显著增高 (P<0 .0 5)。结论 : 结肠细菌总数和双歧杆菌数可能与 LI症状的产生有关 ,同时饮奶习惯可能对双歧杆菌数量有潜在影响。 | 钟燕 黄承钰 Vonk RJ Harmsen HMJ | 2003 | 营养学报2003，25，4： | 8 |
| 4 | Risk Factors for Opportunistic Infections in Patients With Inflammatory Bowel Disease显示文摘 | Murat Toruner Edward V. Loftus W. Scott Harmsen Alan R. Zinsmeister Robert Orenstein William J. Sandborn Jean–Frederic Colombel Laurence J. Egan | 2008 | Gastroenterology2008，，4： | 6 |
| 5 | Model combining pre-transplant tumor biomarkers and tumor size shows more utility in predicting hepatocellular carcinoma recurrence and survival than the BALAD models显示文摘AIM To assess the performance of BALAD, BALAD-2 and their component biomarkers in predicting outcome of hepatocellular carcinoma(HCC) patients after liver transplant.METHODS BALAD score and BALAD-2 class are derived from bilirubin, albumin, alpha-fetoprotein(AFP), Lens culinaris agglutinin-reactive AFP(AFP-L3), and des-gammacarboxyprothrombin(DCP). Pre-transplant AFP, AFP-L3 and DCP were measured in 113 patients transplanted for HCC from 2000 to 2008. Hazard ratios(HR) for recurrence and death were calculated. Univariate and multivariate regression analyses were conducted. C-statistics were used to compare biomarker-based to predictive models. RESULTS During a median follow-up of 12.2 years, 38 patients recurred and 87 died. The HRs for recurrence in patients with elevated AFP, AFP-L3, and DCP defined by BALAD cut-off values were 2.42(1.18-5.00), 1.86(0.98-3.52), and 2.83(1.42-5.61), respectively. For BALAD, the HRs for recurrence and death per unit increased score were 1.48(1.15-1.91) and 1.59(1.28-1.97). For BALAD-2, the HRs for recurrence and death per unit increased class were 1.45(1.06-1.98) and 1.38(1.09-1.76). For recurrence prediction, the combination of three biomarkers had the highest c-statistic of 0.66 vs. 0.64, 0.61, 0.53, and 0.53 for BALAD, BALAD-2, Milan, and UCSF, respectively. Similarly, for death prediction, the combination of three biomarkers had the highest c-statistic of 0.66 vs 0.65,0.61, 0.52, and 0.50 for BALAD, BALAD-2, Milan, and UCSF. A new model combining biomarkers with tumor size at the time of transplant(S-LAD) demonstrated the highest predictive capability with c-statistics of 0.71 and 0.69 for recurrence and death. CONCLUSION BALAD and BALAD-2 are valid in transplant HCC patients, but less predictive than the three biomarkers in combination or the three biomarkers in combination with maximal tumor diameter(S-LAD). | Nicha Wongjarupong Gabriela M Negron-Ocasio Roongruedee Chaiteerakij Benyam D Addissie Essa A Mohamed Kristin C Mara William S Harmsen J Paul Theobald Brian E Peters Joseph G Balsanek Melissa M Ward Nasra H Giama Sudhakar K Venkatesh Denise M Harnois Michael R Charlton Hiroyuki Yamada Alicia Algeciras-Schimnich Melissa R Snyder Terry M Therneau Lewis R Roberts | 2018 | World Journal of Gastroenterology2018，24，12： | 5 |
| 6 | Metastatic Nonfunctioning Pancreatic Neuroendocrine Carcinoma to Liver: Surgical Treatment and Outcomes显示文摘 | Daniel Cusati Lizhi Zhang William S. Harmsen Amy Hu Michael B. Farnell David M. Nagorney John H. Donohue Florencia G. Que Kaye M. Reid-Lombardo Michael L. Kendrick | 2012 | Journal of the American College of Surgeons2012，，1： | 3 |
| 7 | Structural and molecular basis for foot-andmouth disease virus neutralization by two potent protective antibodies显示文摘Dear Editor,Foot-and-mouth disease(FMD)is an economically devastating and highly contagious viral disease of cloven-hoofed animals with a global distribution.The causative agent,FMD virus(FMDV)is a small non-enveloped RNA virus,belonging to the Aphthoviruses genus within Picornaviridae family(Tuthill et al.,2010).Control of FMD has been largely reliant on vaccinations with inactivated virus vaccines.However,significant antigenic diversity within FMDV serotypes and inability of the vaccines to induce immune protection for a long duration of time impinge on the efficacy of available vaccines.The roles of neutralizing antibodies(NAbs)as the principal protective components of the immune responses to FMDV vaccination or infection have been well established(Pay and Hingley,1987;Juleff et al.,2009).Passive immunization of NAbs has also been demonstrated to be effective in curing FMD and many viral diseases(Harmsen et al.,2007;Qiu et al.,2018).A deep understanding of the molecular basis for viral neutralization by antibodies and the identification of key viral epitopes would aid in the development of potent rationally designed broad-spectrum vaccine. | Hu Dong Pan Liu Manyuan Bai Kang Wang Rui Feng Dandan Zhu Yao Sun Suyu Mu Haozhou Li Michiel Harmsen Shiqi Sun Xiangxi Wang Huichen Guo | 2022 | Protein & Cell2022，13，6： | 3 |
| 8 | Risk of Intestinal Cancer in Inflammatory Bowel Disease: A Population-Based Study From Olmsted County, Minnesota显示文摘 | Tine Jess Edward V. Loftus Fernando S. Velayos W. Scott Harmsen Alan R. Zinsmeister Thomas C. Smyrk Cathy D. Schleck William J. Tremaine L. Joseph Melton Pia Munkholm William J. Sandborn | 2006 | Gastroenterology2006，，4： | 3 |
| 9 | Dietary approaches following endoscopic retrograde cholangiopancreatography: A survey of selected endoscopists显示文摘AIM: To describe the dietary recommendations of experienced endoscopists for patients who have undergone endoscopic retrograde cholangiopancreatography (ERCP) and the factors that influence these recommendations. METHODS: Selected U.S. endoscopists with ERCP experience were surveyed by e-mail. A questionnaire with three hypothetical ERCP cases of patients at low, medium and high risk for development of post-ERCP pancreatitis (PEP) was shown. For each scenario, respondents were asked to recommend a post-procedure diet and time to first oral intake. Respondents were also asked about the effect of various clinical factors on their recommendations, including risk of PEP.RESULTS: 97/187 selected ASGE members (51.9%) responded. When risk of PEP was either low, medium or high, 53%, 88% and 96% recommended a diet of clear liquids/NPO respectively, and 2%, 5% and 18% recommended delaying first oral intake until the following day. About 88% of respondents gave the same type of diet to patients at high as those with moderate-risk of PEP (P = 0.04). However, 37% and 43% of respondents gave different types of diet to patients at low vs moderate-risk and low-risk vs high-risk of PEP respectively (P < 0.001). No statistically significant associations were found regarding the effect of other clinical factors or respondent demographics.CONCLUSION: Most experienced endoscopists limit diet to NPO/clear liquids after ERCP for patients at high or moderate risk of post-ERCP pancreatitis. About half allow a low-fat or regular diet in patients at low risk. | Lincoln EVVC Ferreira Mark D Topazian William S Harmsen Alan R Zinsmeister Todd H Baron | 2010 | World Journal of Gastrointestinal Endoscopy2010，2，12： | 3 |
| 10 | Properties, production, and applications of camelid single-domain antibody fragments显示文摘 | M. M. Harmsen H. J. Haard | 2007 | Applied Microbiology and Biotechnology2007，，1： | 2 |
| 11 | Clinical Outcomes for Anaplastic Pancreatic Cancer: A Population-Based Study显示文摘 | Clancy J. Clark Rondell P. Graham Janani S. Arun W. Scott Harmsen Kaye M. Reid-Lombardo | 2012 | Journal of the American College of Surgeons2012，，5： | 2 |
| 12 | 荧光原位杂交技术分析人结肠菌群方法研究显示文摘建立荧光原位杂交技术分析人体内结肠菌群的方法。取受试者新鲜粪便 ,选用 5种特异性的 16SrRNA寡核苷酸探针 ,检测粪便样本收集后的保存时间、温度 ,离心条件及样本固定液存放时间对杂交计数结果的影响。结果建立最佳实验条件为 :粪便样本收集后应尽快在 4℃下保存 ,放置时间不要超过 12小时即作处理 ;样本的适宜离心条件为 70 0g 2分钟 ;样本用多聚甲醛固定后在 - 80℃下存放时间不要超过 5个月。该方法具有较好的稳定性 ,可以有效地检出个体之间结肠菌群的差异。 | 钟燕 黄承钰 阴文娅 Vonk RJ Harmsen HMJ | 2003 | 卫生研究2003，32，3： | 2 |
| 13 | Continuation of metformin use after a diagnosis of cirrhosis significantly improves survival of patients with diabetes显示文摘 | Xiaodan Zhang William S. Harmsen Teresa A. Mettler W. Ray Kim Rosebud O. Roberts Terry M. Therneau Lewis R. Roberts Roongruedee Chaiteerakij | 2014 | Hepatology2014，，6： | 2 |
| 14 | Is There a Role for Endoscopic Therapy as a Definitive Treatment for Post-Laparoscopic Bile Duct Injuries?显示文摘 | Javairiah Fatima Joshua G. Barton Travis E. Grotz Zhimin Geng William S. Harmsen Marianne Huebner Todd H. Baron Michael L. Kendrick John H. Donohue Florencia G. Que David M. Nagorney Michael B. Farnell | 2010 | Journal of the American College of Surgeons2010，，4： | 2 |
| 15 | Peripheral neuropathy incidence in inflammatory bowel disease: A population-based study显示文摘 | Juan J. Figueroa Edward V. Loftus William S. Harmsen P. James B. Dyck Christopher J. Klein | 2013 | Neurology2013，，18： | 2 |
| 16 | Frequency of Extrapancreatic Neoplasms in Intraductal Papillary Mucinous Neoplasm of the Pancreas: Implications for Management显示文摘 | Kaye M. Reid-Lombardo Kellie L. Mathis Christina M. Wood William S. Harmsen Michael G. Sarr | 2010 | Annals of Surgery2010，，1： | 2 |
| 17 | A Randomized, Double-Blinded, Placebo-Controlled Multicenter Trial of Etanercept in the Treatment of Alcoholic Hepatitis显示文摘 | Nicholas C. Boetticher Craig J. Peine Paul Kwo Gary A. Abrams Tushar Patel Bashar Aqel Lisa Boardman Gregory J. Gores William S. Harmsen Craig J. McClain Patrick S. Kamath Vijay H. Shah | 2008 | Gastroenterology2008，，6： | 2 |
| 18 | Relationships Between Disease Activity and Serum and Fecal Biomarkers in Patients With Crohn’s Disease显示文摘 | Jennifer Jones Edward V. Loftus Remo Panaccione Li–Sheng Chen Sandra Peterson Joseph Mcconnell Linnea Baudhuin Karen Hanson Brian G. Feagan Scott W. Harmsen Alan R. Zinsmeister Emelie Helou William J. Sandborn | 2008 | Clinical Gastroenterology and Hepatology2008，，11： | 2 |
| 19 | Aberrant gut microbiota composition at the onset of type 1 diabetes in young children显示文摘 | Marcus C. Goffau Susana Fuentes Bartholomeus Bogert Hanna Honkanen Willem M. Vos Gjalt W. Welling Heikki Hy?ty Hermie J. M. Harmsen | 2014 | Diabetologia2014，，8： | 2 |
| 20 | Results of translabyrinthine removal of 300 acoustic neuromas related to tumor size显示文摘 | Tos M Thomsen J Harmsen A | 1988 | Acta Otolaryngol Suppl1988，452，： | 1 |
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