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| 1 | Identification of tumor associated single-chain Fv by panning and screening antibody phage library using tumor cells显示文摘AIM:To study the feasibility of panning and screeningphage-displaying recombinant single-chain variablefragment (ScFv) of anti-tumor monoclonal antibodiesfor fixed whole cells as the carriers of mAb-bindingantigens.ME,ODS: The recombinant phage displaying librariesfor anti-colorectal tumor mAb MC3Ab, MC5Ab andanti-gastric tumor mAb MGD1 was constructed.Panning and screening were carried outby means ofmodified fixation of colorectal and gastric tumor cellsexpressed the mAb-binding antigens. Concordance ofbinding specificity to tumor cells between phageclones and parent antibodies was analyzed. Thephage of positive clones was identified withcompetitive ELISA, and infected by E.coli HB2151 toexpress soluble ScFv.RESULTS:The ratio of positive clones to MC3-ScF-MC5-ScFv and MGD1-ScFv were 60 %, 24 % and 30 %. MC3-ScFv had Mr 32 000 confirmed by Western blot. Thespecificity to antigen had no difference between 4positive recombinant phage antibodies and MC3Ab.CONCLUSION:The modified process of fixing wholetumor cells is efficient, convenient and feasible to panand screen the phage-displaying ScFv of anti-tumormonoclonal antibodies. | Nie, YZ He, FT Li, ZK Wu, KC Cao, YX Chen, BJ Fan, DM | 2002 | World Journal of Gastroenterology2002，8，4： | 12 |
| 2 | CD147 and VEGF expression in advanced renal cell carcinoma and their prognostic value显示文摘 | Liang YX He HC Han ZD | 2009 | Cancer Invest2009，27，7： | 1 |
| 3 | Study on the efficacy of a new long-acting formulation of N,N-diethyl-m-toluamide(DEET) for the prevention of tick attachment显示文摘 | Salafaky B He YX Li J | 2000 | Am J Trop Med Hyg2000，62，2： | 1 |
| 4 | cD147 and VEGF expression in advanced renal cell carcinoma and their prognostic value显示文摘 | Liang YX He HC Han ZD | 2009 | cancer Invest2009，27，7： | 1 |
| 5 | Magnetic and conductive Fe3O4-polyaniline nanoparticles with core-shell structure 显示文摘 | Deng JG He CL Peng YX | 2003 | Synthetic: Metals2003，139，2： | 1 |
| 6 | Relativity of hippocampal-HPA- PTSD显示文摘 | Chen SY He W Shi YX | 2010 | Chin Foreign Med Treat2010，25，： | 1 |
| 7 | Calcium carbonate nanoparticle delivering vascular endothelial growth factor - C siRNA effectively inhibits lymphan- giogenesis and growth of gastric cancer in vivo显示文摘 | He XW Liu T Chen YX | 2008 | Cancer Gene Therapy2008，15，3： | 1 |
| 8 | Clinical utility of haptoglobin in combination with CEA, NSE and CYFRA21-1 for diagnosis of lung cancer显示文摘 | Wang B He YJ Tian YX | 2014 | Asian Pac J Cancer Prev2014，15，22： | 1 |
| 9 | New technology forcervical cancer screening显示文摘 | Gong JM Shen Y He YX | 2012 | Int J Gynecol Cancer2012，22，9： | 1 |
| 10 | Neural respiratory drive and ventilation in patients with chronic obstructive pulmonary disease during sleep显示文摘 | Luo YM He BT Wu YX | 2014 | Am J Respir Crit Care Med2014，190，2： | 1 |
| 11 | CT findings of adrenal glands in patients with tuberculous Addison's disease 显示文摘 | Wang YX Chen CR He GX | 1998 | J Beige Radiol1998，81，5： | 1 |
| 12 | Prevalence and risk factors of lumbar spondylolisthesis in elderly Chinese men and women 显示文摘 | HE LC WANG YX GONG JS | 2014 | Eur Radiol2014，24，2： | 1 |
| 13 | An active detecting method against SYN flooding attack显示文摘 | Xiao B Chen W He YX | 2005 | 11th IEEE Int''1 Conf on Parallel and Distributed Systems2005，1，： | 1 |
| 14 | Sensitivity of different isolates of Schistosoma japonicum from China to praziquantel显示文摘 | He YX Hu YQ Yu QF | 1992 | Southeast Asian J Trop Med Public Health1992，23，2： | 1 |
| 15 | Detection of autoantibodies against platelet glycoproteins in patients with immune thrombocytopenic purpura by flow cytometric immunobead array显示文摘 | He Y Zhao YX Zhu MQ | 2013 | Clin Chim Acta2013，415，： | 1 |
| 16 | Relationship between platelet counts and cartilage erosion in 436 cases of rheumatoid arthritis 显示文摘 | Zha QL He YT Lu YX | 2006 | Clin Chem Acta2006，371，12： | 1 |
| 17 | Solution structure of anti-HIV-1 and anti-tumor proteins MAP30 structrural insights into its multiple functions 显示文摘 | Wang YX Neamati N Jacob J Palmer I Stahl SJ Kaufman JD Huang PL Winslow HE Pommier Y Wangfield PT Lee-Huang S | 1999 | Cell1999，99，4： | 1 |
| 18 | Parasitological and histopathological studies on rhesus monkeys infected with Chinese mainland strain of Schistosoma japonicum显示文摘 | He YX Yu QF Hu YQ | 1992 | Southeast Asian J Trop Med Public Health1992，23，2： | 1 |
| 19 | Estrogenic G proteincoupled receptor30 signaling is involved in regulation of endometrial carcinoma by promoting proliferation,invasion potential,and interleukin-6 secretion via the MEK/ERK mitogen-activated protein kinase pathway显示文摘 | He YY Cai B Yang YX | 2009 | Cancer Sci2009，100，： | 1 |
| 20 | Influence of ABCB1,CYP3A4*18B and CYP3A5*3 polymorphisms on cyclosporine A pharmacokinetics in bone marrow transplant recipients显示文摘 | Qiu F He XJ Sun YX | 2011 | PHARMACOLOGICAL REPORTS2011，，03： | 1 |
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