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| 1 | Overexpression of acetylcholinesterase inhibited cell proliferation and promoted apoptosis in NRK cells显示文摘AIM: To study the potential function of acetylcholinesterase (ACHE) in apoptosis through overexpression of ACHE in Normal Rat Kidney (NRK) cells. METHODS: AChE activity was detected by the method of Karnovsky and Roots. Activated caspase-3 was analyzed by Western blotting and immunoflurescence with antibody special to activated caspase-3 fragment. The expression plasmids were constructed in pcDNA3.1 containing AChE gene or a fragment of AChE antisense that were got from RT-PCR. Stable expression cell lines were selected by G418 in cells transfected by lipofection. ACHE expression was analyzed by RT-PCR and Western blotting. The proliferation rates of transfected cells were examined by the growth curve and cloning efficiency. MTT assay was used to analyze the cell viability. RESULTS: The proliferation rate of the cells transfected with AChE was retarded and the cloning efficiency was lower (28.2 %±3.1% and 48.7 %±2.1%) than cells transfected with vector (56.1%±0.3%) or AChE-antisense (77.7 %±2.2 %). After 2 d the various clone types were deprived of serum, the residue cell viability were 10.4 %±4.6 % and 12.6 %±6.7 % in the cells transfected with ACHE, and 27.4 %±3.5 % in cells with vector, and 50.3 %±7.8 % in cells with AChE-antisense. CONCLUSION: During apoptosis, increase of AChE protein is to inhibit cell proliferation, and then to promote apoptosis in NRK cells. | Qi-huangJIN Heng-yiHE Yu-fangSHI HeLU Xue-junZHANG | 2004 | Acta Pharmacologica Sinica2004，25，8： | 8 |
| 2 | Machine tool design and operation strategies for green manufacturing显示文摘 | DIAZ N CHOI S HELU M | 2010 | Proceedings of 4th International Conference on High Performance Cutting2010，，： | 1 |
| 3 | Restoration thinning and i-uquence of tree size and leaf area to sapwood area ratio on water relations of Pinus ponderosa 显示文摘 | Simonin K Kolb T E Montes - Helu M | 2006 | Tree Physiology2006，26，4： | 1 |
| 4 | Environ- mental Analysis of Milling Machine Tool Use in Various Manu- facturing Environments显示文摘 | Nancy Diaz Moneer Helu Stephen Jayanathan | 2010 | ISSST2010，16，： | 1 |
| 5 | Automated percutaneous diskectomy显示文摘 | Onik G Helus CA | 1991 | AJK1991，156，： | 1 |
| 6 | Polymetallie sulphide deposit from ' black smoker' chimney: Lau Basin显示文摘 | Hawkins J W Helu S | 1986 | Eos Transactions American Geophysical Union1986，67，： | 1 |
| 7 | Total carote- noid differences in scallop tissues of Chlamys nobilis (Bivalve: Pectinidae) with regard to gender and shell colour 显示文摘 | ZHENG Huaiping LIU Helu ZHANG Tao | 2010 | Food Chem2010，122，4： | 1 |
| 8 | Total carotenoid differences in scallop tissues of Chlamys nobilis (Bivalve:Pectinidae) with regard to gender and shell colour显示文摘 | ZHENG Huaiping LIU Helu ZHANG Tao | 2010 | Food Chem2010，122，4： | 1 |
| 9 | The influence of thinning on components of stand water balance in a ponderosa pine forest stand during and after extreme drought 显示文摘 | Simonin K Kolb T E Montes Helu M | 2007 | Agricuhural and Forest Meteorology2007，143，34： | 1 |
| 10 | Evalua- ting the Relationship between Use Phase Environ- mental Impacts and Manufacturing Process Preci- sion显示文摘 | Helu M Vijayaraghavan A Dornfeld D | 2011 | CIRP Annals-manufacturing Technology2011，60，1： | 1 |
| 11 | Identifying performance assurance challenges for smart manufacturing显示文摘 | HELU M MORRIS K JUNG K | 2015 | Manufacturing Letters2015，6，： | 1 |
| 12 | Stochastic leaderefollower DEA models for two-stage systems显示文摘Data envelopment analysis(DEA)is a non-parametric approach for measuring the relative efficiencies of peer decision making units(DMUs).In recent years,it has been widely used to evaluate two-stage systems under different organization mechanisms.This study modifies the conventional leaderefollower DEA models for two-stage systems by considering the uncertainty of data.The dual deterministic linear models are first constructed from the stochastic CCR models under the assumption that all components of inputs,outputs,and intermediate products are related only with some basic stochastic factors,which follow continuous and symmetric distributions with nonnegative compact supports.The stochastic leaderefollower DEA models are then developed for measuring the efficiencies of the two stages.The stochastic efficiency of the whole system can be uniquely decomposed into the product of the efficiencies of the two stages.Relationships between stochastic efficiencies from stochastic CCR and stochastic leaderefollower DEA models are also discussed.An example of the commercial banks in China is considered using the proposed models under different risk levels. | Zhongbao Zhou Wenting Sun Helu Xiao Qianying Jin Wenbin Liu | 2021 | Journal of Management Science and Engineering2021，6，4： | 0 |
| 13 | Distinct stem/progenitor cells proliferate to regenerate the trachea,intrapulmonary airways and alveoli in COVID-19 patients显示文摘Dear Editor,The global pandemic COVID-19 caused by SARS-CoV-2 virus has infected over 6.5 million individuals and claimed over 350,000 lives worldwide within six months.The respiratory epithelial cells covering the airways and alveoli are the major targets of the virus.Moreover,damage to the epithelium can be exacerbated by mechanical ventilation.It is expected that many of the infected individuals that survived the acute phase will develop pulmonary diseases(e.g.fibrosis)if the epithelium fails to regenerate properly. | Yinshan Fang Helu Liu Huachao Huang Haiyan Li Anjali Saqi Li Qiang Jianwen Que | 2020 | Cell Research2020，30，8： | 0 |
| 14 | Identification of genes responsive to apoptosis in HL-60 cells显示文摘AIM: To identify genes responsive to apoptosis in HL-60 cells treated by homoharringtonine. METHODS: cDNA microarray technology was used to detect gene expression and the result of microarrays for genes (TIEG and VDUP1) was confirmed by Northern analysis. RESULTS: Seventy-five individual mRNAs whose mass changed significantly were identified. Among these genes (25 were up-regulated and 50 were down-regulated), most are known related to oncogenes and tumor suppressor. Some genes were involved in apoptosis signaling pathways. CONCLUSION: TGFβ and TNF apoptosis signaling pathways were initiated during apoptosis in HL-60 cells. TIEG and VDUP1 play important roles in mediating apoptosis. | WeiJIN Le-fengQU PingMIN ShanCHEN HongLI HeLU Yong-taiHOU | 2004 | Acta Pharmacologica Sinica2004，25，3： | 0 |
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