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| 1 | 重组抗原CP_4-EPSPS的表达及免疫学活性研究显示文摘目的:表达并纯化重组CP4-EPSP合成酶(CP4-EPSPS),研究重组CP4-EPSPS的免疫学活性;为进一步制备单克隆抗体和开发检测CP4-EPSPS的快速诊断试剂奠定基础。方法:诱导表达含转基因大豆CP4-EPSPS的重组载体pET-EPSPS,经Ni-NTA琼脂糖树脂亲和层析纯化,以ELISA、胶体金试条、W estern杂交鉴定其免疫活性。结果:重组CP4-EPSPS以包涵体表达为主,上清表达量极低,但胶体金试纸条检测发现上清中CP4-EPSPS抗原活性强;复性包涵体的抗原性与上清的抗原性明显不同。采用扩大培养量、缩小超声破碎重悬体积的方法,从上清中纯化CP4-EPSPS获得成功,ELISA检测此重组抗原免疫小鼠血清抗体滴度可达到1:625,000;W estern杂交证实重组抗原免疫血清与CP4-EPSPS标准品有较好的免疫反应性。结论:包涵体的简单复性方法难以恢复其关键的抗原决定簇的免疫原性;运用胶体金试纸条检测重组CP4-EPSPS活性,灵敏度高,简单快捷,对CP4-EPSPS特异检测相关的关键性抗原决定簇的甄别有重要作用,可指导重组抗原的纯化和在免疫学研究、特别是在单克隆抗体开发中的应用。 | 唐霓 Yu Xiang Helen Ke Zhihui Yao 敬凌霞 黄爱龙 郑建 | 2006 | 重庆医科大学学报2006，31，1： | 4 |
| 2 | Biomanipulation as an Application of Food-Chain Theory: Constraints, Synthesis, and Recommendations for Temperate Lakes显示文摘 | Lars-Anders Hansson Helene Annadotter Eva Bergman Stellan F. Hamrin Erik Jeppesen Timo Kairesalo Eira Luokkanen Per-?ke Nilsson Martin S?ndergaard John Strand | 1998 | Ecosystems1998，，6： | 1 |
| 3 | Relationship Between Intravascular Ultrasound Guidance and Clinical Outcomes After Drug-Eluting Stents: The Assessment of Dual Antiplatelet Therapy With Drug-Eluting Stents (ADAPT-DES) Study显示文摘 | Bernhard Witzenbichler Akiko Maehara Giora Weisz Franz-Josef Neumann Michael J. Rinaldi D. Christopher Metzger Timothy D. Henry David A. Cox Peter L. Duffy Bruce R. Brodie Thomas D. Stuckey Ernest L. Mazzaferri Ke Xu Helen Parise Roxana Mehran Gary S. Min | 2014 | Circulation2014，，4： | 1 |
| 4 | Equilibrium morphology of gas–liquid Janus droplets: A numerical analysis of buoyancy effect显示文摘In this article, a theoretical model for predicting the equilibrium morphology of gas–liquid Janus droplets was built. Based on this model, the effects of bubble radius and volume ratio on morphology change was systematically studied. The increase of bubble radius causes the two parts(bubble and oil drop) in Janus droplets tend to merge while the impact of volume ratio is complicated. When volume ratio increases, these two parts firstly tend to merge, then gradually separate. The accuracy of this model was verified by experimental results. | Shaobin Zhang Helen Yan Yuhao Geng Ke Wang Jianhong Xu | 2018 | Chinese Journal of Chemical Engineering2018，26，10： | 1 |
| 5 | IL - 14 and IL - 16 are expressed in the thyroid of patients with either Graves’ dis- ease or Hashimoto thyroiditis显示文摘 | Helen KE Ajjan RA Metcalfe RA | 2015 | Clinical Endocrinology2015，83，5： | 1 |
| 6 | In silico directed mutagenesis identifies the CD 81/claudin‐1 hepatitis C virus receptor interface显示文摘 | Christopher Davis Helen J. Harris Ke Hu Heidi E. Drummer Jane A. McKeating Jonathan G. L. Mullins Peter Balfe | 2012 | Cell Microbiol2012，，12： | 1 |
| 7 | Quantification and Impact of Untreated Coronary Artery Disease After Percutaneous Coronary Intervention显示文摘 | Philippe Généreux Tullio Palmerini Adriano Caixeta Gregg Rosner Philip Green Ovidiu Dressler Ke Xu Helen Parise Roxana Mehran Patrick W. Serruys Gregg W. Stone | 2012 | Journal of the American College of Cardiology2012，，24： | 1 |
| 8 | Biomanipulation as an Application of Food-Chain Theory: Constraints, Synthesis, and Recommendations for Temperate Lakes显示文摘 | Lars-Anders Hansson Helene Annadotter Eva Bergman Stellan F. Hamrin Erik Jeppesen Timo Kairesalo Eira Luokkanen Per-?ke Nilsson Martin S?ndergaard John Strand | 1998 | Ecosystems1998，，6： | 1 |
| 9 | Stability of partially deacetylated chitin nano-fiber dispersions mediated by protonic acids显示文摘Partially deacetylated chitin nano-fiber(DAChN)dispersions were prepared using mechanical treatment of partially deacetylated crab shellα-chitin under acidic conditions mediated by various protonic acids.The representative organic acids had a better efficiency in terms of mediating the nanofibrillation of chitin fibers in comparison to the inorganic acid(HCl).The DAChN dispersed in water at a pH of 3.5 mediated by gluconic acid exhibited the highest yield of nano-fibers(88.6%),followed by succinic,itaconic,and ascorbic acids with a nano-fiber yield of 79.9%,73.3%and 66.0%,respectively.The pH,conductivity,viscosity,and light transmittance of dispersions were assessed under different storage times.All of the tested DAChN dispersions were stable for at least 15 weeks at 4℃. | Zhiguo Wang Hui Li Liang Liu Jie Jiang Ke Zheng Helen Ocampo Yimin Fan | 2016 | Journal of Bioresources and Bioproducts2016，1，3： | 0 |
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