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| 1 | Development of A Super-Sensitive Diagnostic Method for African Swine Fever Using CRISPR Techniques显示文摘African swine fever(ASF)is an infectious disease caused by African swine fever virus(ASFV)with clinical symptoms of high fever,hemorrhages and high mortality rate,posing a threat to the global swine industry and food security.Quarantine and control of ASFV is crucial for preventing swine industry from ASFV infection.In this study,a recombinase polymerase amplification(RPA)-CRISPR-based nucleic acid detection method was developed for diagnosing ASF.As a highly sensitive method,RPA-CRISPR can detect even a single copy of ASFV plasmid and genomic DNA by determining fluorescence signal induced by collateral cleavage of CRISPR-lw Cas13 a(previously known as C2c2)through quantitative real-time PCR(q PCR)and has the same or even higher sensitivity than the traditional q PCR method.A lateral flow strip was developed and used in combination with RPA-CRISPR for ASFV detection with the same level of sensitivity of Taq Man q PCR.Likewise,RPA-CRISPR is capable of distinguishing ASFV genomic DNA from viral DNA/RNA of other porcine viruses without any cross-reactivity.This diagnostic method is also available for diagnosing ASFV clinical DNA samples with coincidence rate of 100%for both ASFV positive and negative samples.RPA-CRISPR has great potential for clinical quarantine of ASFV in swine industry and food security. | Meishen Ren Hong Mei Ming Zhou Zhen F.Fu Heyou Han Dingren Bi Fuhu Peng Ling Zhao | 2021 | Virologica Sinica2021，36，2： | 5 |
| 2 | One step growth of high luminescence CdTe quantum dots with low cytotoxicity in ambient atmospheric conditions显示文摘 | Sheng Zonghai Han Heyou Hu Xiaofeng | | 0，，： | 1 |
| 3 | A novel method for the determination of Pb2+ based on the quenching of the fluorescence of CdTe quantum dots显示文摘 | Wu Hongmin Liang Jiangong Han Heyou | 2008 | Microchim Acta2008，161，： | 1 |
| 4 | Facile synthesis of fluorescent carbon dots using wa- termelon peel as a carbon source 显示文摘 | Zhou Jiaojiao Sheng Zonghai Han Heyou | 2012 | Matter Lett2012，66，1： | 1 |
| 5 | One-step synthesis of water-sol- uble ZnSe quantum dots via microwave irradiation 显示文摘 | Huang Liang Han Heyou | 2010 | Materials Letters2010，64，9： | 1 |
| 6 | Direct electrochemil of CdTe quantum dots based on room temperature ionic liquid film and high sensitivity sensing of gossypol 显示文摘 | HUA Lijuan ZHOU Jiaojiao HAN Heyou | 2010 | Electrochimica Acta2010，55，3： | 1 |
| 7 | An ultrasensitive method for the detection of gene fragment from transgenics using label-free gold nanoparticle probe and dynamic light scattering显示文摘 | Duyang Gao Zonghai Sheng Heyou Han | 2011 | Analytica Chimica Acta2011，，1： | 1 |
| 8 | Utilization of waste freshwater mussel shell as an economic catalyst for biodiesel production显示文摘 | Hu Shengyang Wang Yun Han Heyou | 2011 | Biomass and Bioenergy2011，35，8： | 1 |
| 9 | Synthesis of Biodiesel from Rapeseed Oil Using K_2O/γ-Al_2O_3 as Nano-Solid-Base Catalyst显示文摘Nano-solid-base catalyst K2O/γ-Al2O3 was prepared and adopted for the synthesis of biodiesel by transesterification of rapeseed oil with methanol. The particle diameter of the catalyst was about 50 nm, which was measured by transmission electron microscopy (TEM). The variables affecting the yield of biodiesel during transesterification, such as mass ratio of KNO3 to γ-Al2O3, calcination temperature, calcination time, catalyst content, molar ratio of methanol to oil, reaction temperature and reaction time were investigated. The catalyst obtained by calcining a mixture of KNO3 and γ-Al2O3 ( mK NO 3 / mγ -Al 2 O3= 70 %)at 600℃ for 3 h, was found to be the optimum one, which gave the highest catalytic activity in the reaction. With 3% (mcatalyst/moil) catalyst, when the transesterification was carried out at a molar ratio of methanol to oil of 12∶:1, a reaction temperature of 70 ℃ , and a reaction time of 3 h, yield of 94% was achieved. | HAN Heyou GUAN Yanping | 2009 | Wuhan University Journal of Natural Sciences2009，14，1： | 1 |
| 10 | Nucleobase,nucleoside,nucleotide,and oligonucleotide coordinated metal ions for sensing and biomedicine applications显示文摘Metal ions play critical roles in chemical,biological,and environmental processes.Various biomolecules have the ability to coordinate with metal ions and form various materials.Nucleobases,nucleosides,and nucleotides,as the essential components of DNA,have emerged as a useful building block for the construction of functional nanomaterials.In recent years,DNA oligonucleotides have also been used for this purpose.We herein review the strategies for the synthesis of soft nanomaterials through the assembly of nucleotides(or DNA)and metal ions to yield various nanoparticles,fibers,and hydrogels.Such coordination methods are simple to operate and can be carried out under ambient conditions.The luminescent,catalytic,and molecular recognition properties of these coordination materials are described with representative recent examples.Their applications ranging from biosensing,enzyme encapsulation,catalysis,templated shell growth to cancer therapy are highlighted.Finally,challenges of this field and future perspectives are discussed. | Jiaojiao Zhou Heyou Han Juewen Liu | 2022 | Nano Research2022，15，1： | 1 |
| 11 | Probingthe interaction of magnetic iron oxide nanoParticleswith bovine serum albumin by sPectroscoPic techniques显示文摘 | Yang Qinqin Liang Jiangong Han Heyou | 2009 | J Phys Chem B2009，113，10： | 1 |
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