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| 1 | New combination test for hepatitis C virus genotype and viral load determination using Amplicor GT HCV MONITOR test v2.0显示文摘AIM: To develop a new sensitive and inexpensive hepatitis C virus (HCV) combination test (HCV Guideline test) that enables the determination of HCV genotypes 1, 2 and 3,and simultaneous determination of HCV viral load using commercial Amplicor GT HCV MONITOR test v2.0 (microwell version).METHODS: The HCV Guideline test used the PCR product generated in commercial Amplicor GT HCV Monitor test v2.0 for viral load measurement using microwell plate version of Amplicor HCV Monitor and also captured on separate plates containing capture probes and competitive oligonucleotide probes specific for HCV genotypes 1, 2 and 3, The HCV genotype was subsequently determined using the biotin-labeled PCR product and five biotin-labeled HCV-specific probes.RESULTS: The sensitivity of the HCV Guideline test was 0.5 KIU/mL. Specificity of the HCV Guideline test was confirmed by direct sequencing of HCV core region and molecular evolutionary analyses based on a panel of 31 samples. The comparison of the HCV Guideline test and an in-house HCV core genotyping assay using 252 samples from chronic hepatitis C patients indicated concordant results for 97.2% of samples (59.5% genotype 1, 33.7% genotype 2, 6.0% genotype 3, and 0.8% mixed genotypes).Similarly, the HCV Guideline test showed concordance with a serological test, and the serological test failed to assign any serotype in 12.7% of the samples, indicating a better sensitivity of the HCV Guideline test.CONCLUSION: Clinically, both viral load and genotypes (1, 2 and 3) have been found to be major predictors of antiviral therapy outcome regarding chronic hepatitis C based on guidelines and they are, in normal circumstances,performed as separate stand-alone assays. The HCV Guideline test is a useful method for screening large cohorts in a routine clinical setting for determining the treatment regimen and for predicting the outcome of antiviral therapy of chronic hepatitis C. | Motokazu Mukaide Yasuhito Tanaka Hirokazu Kakuda Kei Fujiwara Fuat Kurbanov Eturo Orito Kentaro Yoshioka Kiyotaka Fujise Shoji Harada Takazumi Kozaki Kazuo Takemura Kazumasa Hikiji Masashi Mizokami | 2005 | World Journal of Gastroenterology2005，11，4： | 3 |
| 2 | Experimental study of reconstruction of the temporomandibular joint using a bone unmepert technique显示文摘 | Takato T Matsumoto S | 2000 | J Oral Maxillofac Surg2000，58，11： | 1 |
| 3 | Electrolyte analysis of pleural effusion as an indicator of drowning in seawater and freshwater显示文摘 | Usumoto Y Sameshima N Hikiji W | 2009 | J For- ensic Leg Med2009，16，6： | 1 |
| 4 | Naomi Sameshima Estimation of postmortem interval based on the speetrophotometrie analysis of postmortem lividity显示文摘 | Usumoto Y Hikiji W | 2010 | Legal Medicine2010，10，： | 1 |
| 5 | Construction of calibration-locking databases for rapid and reliable drug screening by gas chromatography-mass spectrometry显示文摘 | Keiko Kudo Tomomi Ishida Wakako Hikiji Makiko Hayashida Kyoko Uekusa Yosuke Usumoto Akiko Tsuji Noriaki Ikeda | 2009 | Forensic Toxicology2009，，1： | 1 |
| 6 | Experimental study of re- construction of the temporomandibular joint using a bone trans- port technique 显示文摘 | Hikiji H Takato T Matsumoto S | 2000 | J Oral Maxillofac Surg2000，58，12： | 1 |
| 7 | Point mutations of rhodopsin gene found in Japanese families with autosomal dominant retinitis pigmentosa (ADRP)显示文摘 | Keiko Fujiki Yoshihiro Hotta Mutsuko Hayakawa Hitoshi Sakuma Takashi Shiono Michiru Noro Takehiko Sakuma Makoto Tamai Kazumasa Hikiji Ryuji Kawaguchi Akihiko Hoshi Akira Nakajima Atsushi Kanai | 1992 | The Japanese Journal of Human Genetics1992，，2： | 1 |
| 8 | A distinctive role of the leukotriene B4 receptor BLT 1 in osteoclastic activity during bone loss显示文摘 | Hikiji H Ishii S Yokomizo T | 2009 | Proc Natl Acad Sci U S A2009，106，21： | 1 |
| 9 | A simple and sensitive method for the determination of propofol in human solid tissues by gas chromatography-mass spectrometry 显示文摘 | HIKIJI W KUDO K USUMOTO Y | 2010 | J Anal Toxicol2010，34，7： | 1 |
| 10 | Experimental study of reconstruction of the temporomandibular joint using a bone transport technique显示文摘 | Takato T Matsumoto S | 2000 | J Oral Maxillofac Surg2000，58，11： | 1 |
| 11 | , Fatal arnniotic fluid embolism with typical pathohistological, histochemical and clinical features显示文摘 | Hikiji W | 2013 | Forensic Sci Int2013，226，13： | 1 |
| 12 | The vertical structure of fog observed with a lidar system at Misawa Airbase,Japan 显示文摘 | TOMINE K MICHIMOTO K HIKIJI I | 1991 | Appl Meteor1991，30，： | 1 |
| 13 | Peroxynitrite production by TNF-alpha and IL-1bate: implication for suppression of osteoblastic differentiation 显示文摘 | Hikiji H Shin WS Koizumi T | 2000 | Am J Physiol Endocrinol Metab2000，278，6： | 1 |
| 14 | Construction of calibration-locking databases for rapid and reliable drug screening by gaschromatography-mass spectrometry 显示文摘 | KUDO K ISHIDA T HIKIJI W | 2009 | Forensic Toxicology2009，27，1： | 1 |
| 15 | Experimental study of reconstruction of the temporomandibular joint using a bone transport technique 显示文摘 | Hikiji H Takato T Matsumoto S | 2000 | J Oral Maxillofac Surg2000，58，11： | 1 |
| 16 | Experimental study of reconstruction of the temporomandibular joint using a bone transport technique显示文摘 | Hikiji H Takato T Matsumoto S | 2000 | J Oral Maxillofac Surg2000，58，11： | 1 |
| 17 | Direct action of nitric oxide on osteoblast differentiation 显示文摘 | HIKIJI H SHIN W S OIDA S | 1997 | FEBS Letters1997，410，1： | 1 |
| 18 | Estimation of postmortem interval based on the speetrophotometrie analysis of postmortem lividity显示文摘 | Usumoto Y Hikiji W Sameshima N | 2010 | Leg Med(Tokyo)2010，12，1： | 1 |
| 19 | Peroxynitrite production by TNF-alpha and IL-lbeta: implication for suppression of osteoblastic differentiation 显示文摘 | Hikiji H Shin WS Koizumi T | 2000 | Am J Physiol Endocrinol Metab2000，278，： | 1 |
| 20 | An Autopsy Case of Suspected Anti‑N‑Methyl‑D‑Aspartate Receptor Encephalitis显示文摘N-methyl-D-aspartate receptor(NMDAR)encephalitis is a rare type of autoimmune limbic encephalitis.Herein,we report an autopsy case of highly suspected anti‑NMDAR encephalitis,based on the patient’s past psychiatric history and autopsy findings of bilateral hippocampal sclerosis and ovarian teratoma.We found that it can be difficult to accurately diagnose autoimmune encephalitis at postmortem forensic autopsy without a case history and background information. | Kino Hayashi Wakako Hikiji Yohsuke Makino Hisaomi Suzuki | 2019 | Journal of Forensic Science and Medicine2019，5，4： | 0 |
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