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| 1 | Effects of salvianolic acid-A on NIH/3T3 fibroblast proliferation,collagen synthesis and gene expression显示文摘AIM To investigate the mechanisms ofsalvianolic acid A(SA-A)against liver fibrosisin vitro.METHODS NIH/3T3 fibroblasts were culturedroutinely,and incubated with 10-4mol/L-10-7mol/L SA-A for 22 h.The cell viability wasassayed by[3H]proline incorporation,cellproliferation by[3H]TdR incorporation,cellcollagen synthetic rate was measured with[3H]proline impulse and collagenase digestionmethod.The total RNA was prepared from thecontrol cells and the drug treated cellsrespectively,and α(1)I pro-collagen mRNAexpression was semi-quantitatively analyzedwith RT-PCR.RESULTS 10-4mol/L SA-A decreased cellviability and exerted some cytotoxiciy,while10-5mol/L-10-7mol/L SA-A did not affect cellviability,but inhibited cell proliferationsignificantly,and 10-6mol/L SA-A had the besteffect on cell viability among theseconcentrations of drugs.10-5mol/L-10-6mol/LSA-A inhibited intracellular collagen syntheticrate,but no significant influence on extracellularcollagen secretion.Both 10-5mol/L and10-6mol/L SA-A could decrease α(1)I pro-collagen mRNA expression remarkably.CONCLUSION SA-A had potent action againstliver fibrosis.It inhibited NIH/3T3 fibroblastproliferation,intracellular collagen syntheticrate and type I pro-collagen gene expression,which may be one of the main mechanisms of thedrug. | Liu CH Hu YY Wang XL Liu P Xu LM | 2000 | World Journal of Gastroenterology2000，6，3： | 25 |
| 2 | Scutellarin protects oxygen/glucose-deprived astrocytes and reduces focal cerebral ischemic injury显示文摘Scutellarin, a bioactive flavone isolated from Scutellaria baicalensis, has anti-inflammatory, anti-neurotoxic, anti-apoptotic and anti-oxidative effects and has been used to treat cardiovascular and cerebrovascular diseases in China. However, the mechanisms by which scutellarin mediates neuroprotection in cerebral ischemia remain unclear. The interaction between scutellarin and nicotinamide adenine dinucleotide phosphate oxidase 2(NOX2) was assessed by molecular docking study, which showed that scutellarin selectively binds to NOX2 with high affinity. Cultures of primary astrocytes isolated from the cerebral cortex of neonatal Sprague-Dawley rats were pretreated with 2, 10 or 50 μM scutellarin for 30 minutes. The astrocytes were then subjected to oxygen/glucose deprivation by incubation for 2 hours in glucose-free Dulbecco's modified Eagle's medium in a 95% N2/5% CO_2 incubator, followed by simulated reperfusion for 22 hours. Cell viability was assessed by cell counting kit-8 assay. Expression levels of NOX2, connexin 43 and caspase-3 were assessed by western blot assay. Reactive oxygen species were measured spectrophotometrically. Pretreatment with 10 or 50 μM scutellarin substantially increased viability, reduced the expression of NOX2 and caspase-3, increased the expression of connexin 43, and diminished the levels of reactive oxygen species in astrocytes subjected to ischemia-reperfusion. We also assessed the effects of scutellarin in vivo in the rat transient middle cerebral artery occlusion model of cerebral ischemia-reperfusion injury. Rats were given intraperitoneal injection of 100 mg/kg scutellarin 2 hours before surgery. The Bederson scale was used to assess neurological deficit, and 2,3,5-triphenyltetrazolium chloride staining was used to measure infarct size. Western blot assay was used to assess expression of NOX2 and connexin 43 in brain tissue. Enzyme-linked immunosorbent assay was used to detect 8-hydroxydeoxyguanosine(8-OHd G), 4-hydroxy-2-nonenal(4-HNE) and 3-nitrotyrosin(3-NT) in brain tissue. Immunofluorescence double staining was used to determine the co-expression of caspase-3 and Neu N. Pretreatment with scutellarin improved the neurological function of rats with focal cerebral ischemia, reduced infarct size, diminished the expression of NOX2, reduced levels of 8-OHd G, 4-HNE and 3-NT, and reduced the number of cells co-expressing caspase-3 and Neu N in the injured brain tissue. Furthermore, we examined the effect of the NOX2 inhibitor apocynin. Apocynin substantially increased connexin 43 expression in vivo and in vitro. Collectively, our findings suggest that scutellarin protects against ischemic injury in vitro and in vivo by downregulating NOX2, upregulating connexin 43, decreasing oxidative damage, and reducing apoptotic cell death. | Jing-Bo Sun Yan Li Ye-Feng Cai Yan Huang Shu Liu Patrick KK Yeung Min-Zhen Deng Guang-Shun Sun Prince LM Zilundu Qian-Sheng Hu Rui-Xin An Li-Hua Zhou Li-Xin Wang Xiao Cheng | 2018 | Neural Regeneration Research2018，13，8： | 16 |
| 3 | Stem cell factor and c-kit are involved in hepatic recovery after acetaminophen-induced liver injury in mice显示文摘 | Hu B Colletti LM | 2008 | Am J Physiol Gastrointest Liver Physiol2008，295，： | 1 |
| 4 | Current insights into the autotrophic thaumarchaeal ammonia oxidation in acidic soils显示文摘 | He JZ Hu HW Zhang LM | 2012 | Soil Biology and Biochemistry2012，55，： | 1 |
| 5 | IL-22 is involved in liver regeneration after hepatectomy 显示文摘 | Ren XD Hu B Colletti LM | 2010 | Am J Physiol Gastrointest Liver Physiol2010，298，1： | 1 |
| 6 | Flexural Properties of3D- SiO_,/SiO, Composites 显示文摘 | Chen H Zhang LM Hu LM | 2003 | Key Engineering Materials2003，249，： | 1 |
| 7 | EPO reduces reactive gliosis and stimulates neurotrophin expression in Muller cells显示文摘 | Hu LM Luo Y Zhang J | | 0，，： | 1 |
| 8 | A novel double-antigensandwich enzyme-linked immunosorbent assay for measurement ofantibodies against rabies virus显示文摘 | Yang LM Zhao LZ Hu RL | 2006 | Clin Vaccine Immunol2006，13，8： | 1 |
| 9 | KLF family members regulate intrinsic axon regeneration ability显示文摘 | Darcie LM Murray GB Hu Y | 2009 | Science2009，326，5950： | 1 |
| 10 | Separation of four flavonoids from Rhodiola rosea by on-line combination of sample preparation and counter-current chromatography显示文摘 | Ma CY Hu LM Fu QY | 2013 | Journal of Chromatography A2013，1306，1： | 1 |
| 11 | Dissimilarity ofphytoplankton assemblages in two connected tropical reservoirs: effectsof water transportation and environmental filtering 显示文摘 | Xiao LJ Hu R Peng L Lei LM Feng Y Han BP | 2016 | Hydrobiologia2016，764，1： | 1 |
| 12 | Overexpression of PDZ1domain prevents apoptosis of rat hippocampal neurons induced bykainic acid显示文摘 | HU SQ ZONG YY FAN LM | 2009 | Neurosci Lett2009，460，2： | 1 |
| 13 | Chronic unpredict- able stress decreases expression of brain-derived neuro- trophic factor (BDNF) in mouse ovaries: relationship to oocytes developmental potential 显示文摘 | Wu LM Hu MH Tong XH | 2012 | PLoS One2012，7，52： | 1 |
| 14 | Protective effects of apocynin on 'two-hit' injury induced by hemorrhagic shock and lipopolysaccharide显示文摘 | Zhou R Hu DY Liu LM | 2002 | J Acta Pharmacol Sin2002，23，11： | 1 |
| 15 | Subclass opioid receptors associated with the cardiovascular depression after traumat- ic shock and the antishock effects of its specific receptor antagonists显示文摘 | Liu LM Hu DY Pan XK | 2005 | Shock2005，24，5： | 1 |
| 16 | Clinical analysis of 68 pa- tients with pulmonary mycosis in China 显示文摘 | Luo BL Zhang LM Hu CP | 2011 | Multidiscip Re- spir Med2011，6，5： | 1 |
| 17 | Lateral habenula lesions improve the behavioral response in depressed rats via increasing the serotonin level in dorsal raphe nucleus 显示文摘 | Yang LM Hu B Xia YH | 2008 | Behav Brain Res2008，188，1： | 1 |
| 18 | Isolation of oligomeric stilbenes from Caragana sinica 显示文摘 | Kulanthaivel P Janzen WP Bahias LM Jiang JB Hu CQ Darges JW et aL Naturally occurring pro- tein kinase C inhibitors II | 1995 | Planta Med1995，61，1： | 1 |
| 19 | Molecular approaches identify known species,reveal cryptic species and verify host specificity of Chinese Philotrypesis(Hymenoptera:Pteromalidae)显示文摘 | Zhou M J Xiao JH Bian SN Li YW Niu LM Hu HY Wu WS Murphy RW Huang DW | | 0，，04： | 1 |
| 20 | Serum microRNA profiles serve as novel biomarkers for HBV infection and diagnosis of HBV-positive hepatocarcinoma 显示文摘 | Li LM Hu ZB Zhou ZX | 2010 | Cancer Res2010，70，23： | 1 |
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