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| 1 | Isolation and characterization of H7N9 viruses from live poultry markets--Implication of the source of current H7N9 infection in humans显示文摘On March 31, 2013, the National Health and Family Planning Commission announced that human infections with a previously undescribed influenza A (H7N9) virus had occurred in Shanghai and Anhui Province, China. To investigate the possible origins of the H7N9 viruses causing these human infections, we collected 970 samples, including drinking water, soil, and cloacal and tracheal swabs of poultry from live poultry markets and poultry farms in Shanghai and Anhui Province. Twenty samples were positive for the H7N9 influenza virus. Notably, all 20 viruses were isolated from samples collected from live poultry markets in Shanghai. Phylogenetic analyses showed that the six internal genes of these novel human H7N9 viruses were derived from avian H9N2 viruses, but the ancestor of their HA and NA genes is uncertain. When we examined the phylogenetic relationship between the H7N9 isolates from live poultry markets and the viruses that caused the human infections, we found that they shared high homology across all eight gene segments. We thus identified the direct avian origin of the H7N9 influenza viruses that caused the human infections. Importantly, we observed that the H7N9 viruses isolated from humans had acquired critical mutations that made them more 'human-like'. It is therefore imperative to take strong measures to control the spread of H7N9 viruses in birds and humans to prevent further threats to human health. | SHI JianZhong DENG GuoHua LIU PeiHong ZHOU JinPing GUAN LiZheng LI WenHui LI XuYong GUO Jing WANG GuoJun FAN Jun WANG JinLiang LI YuanYuan JIANG YongPing LIU LiLing TIAN GuoBin LI ChengJun CHEN HuaLan | 2013 | Chinese Science Bulletin2013，58，16： | 85 |
| 2 | Honeysuckle-encoded atypical microRNA2911 directly Largets influenza A viruses显示文摘 | Zhen Zhou Xihan Li Jinxiong Liu Lei Dong Qun Chen Jialing Liu Huihui Kongt Qianyi Zhang Xian Qi Dongxia Hou Lin Zhang Guoquan Zhang Yuchen Liu Yujing Zhang Jing Li Jin Wang Xi Chen Hua Wang Junfeng Zhang Hualan Chen Ke Zen Chen-Yu Zhang | 2015 | Cell Research2015，25，1： | 104 |
| 3 | Clinical observation of salvianolic acid B in treatment of liver fibrosis in chronic hepatitis B显示文摘AIM: To evaluate the clinical efficacy of salvianolic acidB (SA-B) on liver fibrosis in chronic hepatitis B.METHODS: Sixty patients with definite diagnosis of liverfibrosis with hepatitis B were included in the trial.Interferon-γ (IFN-γ) was used as control drug. Thepatients took orally SA-B tablets or received muscularinjection of IFN-γ in the double blind randomized test,The complete course lasted 6 months. The histologicalchanges of liver biopsy specimen before and after thetreatment were the main evidence in evaluation, incombination with the results of contents of serum HA,LN, Ⅳ-C, P-Ⅲ-P, liver ultrasound imaging, andsymptoms and signs.RESULTS: Reverse rate of fibrotic stage was 36.67 % inSA-B group and 30.0 % in IFN-γgroup. Inflammatoryalleviating rate was 40.0 % in SA-B group and 36.67 %in IFN-γ group. The average content of HA and Ⅳ-Cwas significantly lower than that before treatment. Theabnormal rate also decreased remarkably. Overallanalysis of 4 serological fibrotic markers showedsignificant improvement in SA-B group as comparedwith the IFN-γgroup. Score of liver ultrasound imagingwas lower in SA-B group than in IFN-γgroup (HA 36.7 %vs80 %,Ⅳ-C 3.3 % vs23.2 %). Before the treatment,ALT AST activity and total bilirubin content of patientswho had regression of fibrosis after oral administrationof SA-B, were significantly lower than those of patientswho had aggravation of fibrosis after oraladministration of SA-B. IFN-γ showed certain sideeffects (fever and transient decrease of leukocytes,occurrence rates were 50 % and 3.23 %), but SA-Bshowed no side effects.CONCLUSION: SA-B could effectively reverse liverfibrosis in chronic hepatitis B. SA-B was better than IFN-γ in reduction of serum HA content, overall decrease of4 serum fibrotic markers, and decrease of ultrasoundimaging score. Liver fibrosis in chronic hepatitis B withslight liver injury was more suitable to SA-B in anti-fibrotic treatment. SA-B showed no obvious side effects. | Ping Liu Yi-Yang Hu Cheng Liu Hui-Ming Xue Zhi-Qiang Xu Lie-Ming Xu Cheng-Hai Liu Hong-Tu Gu Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China Da-Yuan Zhu Shanghai Institute of Metaria Medica,Chinese Academy of Sciences,Shanghai 200031,China Zhi-Qing Zhang the 4~(th) Hualyin City Hospital,Hualan City,223000,JiangSu Province,China | 2002 | World Journal of Gastroenterology2002，8，4： | 85 |
| 4 | H7N9 virulent mutants detected in chickens in China pose an increased threat to humans显示文摘 | Jianzhong Shi Guohua Deng Huihui Kong Chunyang Gu Shujie Ma Xin Yin Xianying Zeng Pengfei Cui Yan Chen Huanliang Yang Xiaopeng Wan Xiurong Wang Liling Liu Pucheng Chen Yongping Jiang Jinxiong Liu Yuntao Guan Yasuo Suzuki Mei Li Zhiyuan Qu Lizheng Guan Jinkai Zang Wenli Gu Shuyu Han Yangming Song Yuzhen Hu Zeng Wang Linlin Gu Wenyu Yang Libin Liang Hongmei Bao Guobin Tian Yanbing Li Chuanling Qiao Li Jiang Chengjun Li Zhigao Bu Hualan Chen | 2017 | Cell Research2017，27，12： | 70 |
| 5 | H5N1 avian influenza in China显示文摘H5N1 highly pathogenic avian influenza virus was first detected in a goose in Guangdong Province of China in 1996. Multiple genotypes of H5N1 viruses have been identified from apparently healthy waterfowl since 1999. In the years 2004-2008, over 100 outbreaks in domestic poultry occurred in 23 provinces and caused severe economic damage to the poultry industry in China. Beginning from 2004, a culling plus vaccination strategy has been implemented for the control of epidemics. Since then, over 35420000 poultry have been depopulated, and over 55 billion doses of the different vaccines have been used to control the outbreaks. Although it is logistically impossible to vaccinate every single bird in China due to the large poultry population and the complicated rearing styles, there is no doubt that the increased vaccination coverage has resulted in decreased disease epidemic and environmental virus loading. The experience in China suggests that vaccination has played an important role in the protection of poultry from H5N1 virus infection, the reduction of virus load in the environment, and the prevention of H5N1 virus transmission from poultry to humans. | CHEN HuaLan Animal Influenza Laboratory of the Ministry of Agriculture and National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China | 2009 | Science China(Life Sciences)2009，52，5： | 36 |
| 6 | Vaccination of poultry successfully eliminated human infection with H7N9 virus in China显示文摘The H7 N9 viruses that emerged in China in 2013 were nonpathogenic in chickens but mutated to a highly pathogenic form in early 2017 and caused severe disease outbreaks in chickens. The H7 N9 influenza viruses have caused five waves of human infection, with almost half of the total number of human cases(766 of 1,567) being reported in the fifth wave, raising concerns that even more human infections could occur in the sixth wave. In September 2017, an H5/H7 bivalent inactivated vaccine for chickens was introduced, and the H7 N9 virus isolation rate in poultry dropped by 93.3% after vaccination. More importantly,only three H7 N9 human cases were reported between October 1, 2017 and September 30, 2018, indicating that vaccination of poultry successfully eliminated human infection with H7 N9 virus. These facts emphasize that active control of animal disease is extremely important for zoonosis control and human health protection. | Xianying Zeng Guobin Tian Jianzhong Shi Guohua Deng Chengjun Li Hualan Chen | 2018 | Science China(Life Sciences)2018，61，12： | 37 |
| 7 | H5N1 influenza viruses: outbreaks and biological properties显示文摘流行性感冒的所有已知的子类型 A 病毒在野水鸟被维持,这些病毒的自然水库。流行性感冒 A 病毒被孤立从许多有改变病态和死亡率的动物种类。更重要地,流行性感冒 A 病毒与潜在地致命的结果在人引起呼吸疾病。在人的本地或全球的爆发被过量住院和死亡典型地描绘。在 1997, H5N1 子类型的高度病原的鸟的流行性感冒病毒在传给人的香港出现了,导致由鸟的流行性感冒病毒感染的人的死亡的首先记录的盒子。在越南,印度尼西亚,和泰国在家禽在 2003 年 7 月开始的新爆发,和高度病原的鸟的 H5N1 流行性感冒病毒后来在整个亚洲并且进欧洲和非洲传播了。这些病毒继续与高死亡率感染人并且引起隐约可见的世界范围的担心流行。而且, H5N1 病毒爆发在整个亚洲在家禽工业上有破坏效果。因为 H5N1 病毒爆发看起来从南部的中国发源,我们这里在中国检验 H5N1 流行性感冒病毒,与他们的生物性质上的一个重音。 | Gabriele Neuman Hualan Chen George F Gao Yuelong Shu Yoshihiro Kawaoka | 2010 | Cell Research2010，20，1： | 19 |
| 8 | Genetic and biological characteristics of the globally circulating H5N8 avian influenza viruses and the protective efficacy offered by the poultry vaccine currently used in China显示文摘The H5N8 avian influenza viruses have been widely circulating in wild birds and are responsible for the loss of over 33 million domestic poultry in Europe, Russia, Middle East, and Asia since January 2020. To monitor the invasion and spread of the H5N8 virus in China, we performed active surveillance by analyzing 317 wild bird samples and swab samples collected from 41,172 poultry all over the country. We isolated 22 H5N8 viruses from wild birds and 14 H5N8 viruses from waterfowls. Genetic analysis indicated that the 36 viruses formed two different genotypes: one genotype viruses were widely detected from different wild birds and domestic waterfowls;the other genotype was isolated from a whopper swan. We further revealed the origin and spatiotemporal spread of these two distinct H5N8 virus genotypes in 2020 and 2021. Animal studies indicated that the H5N8 isolates are highly pathogenic to chickens, mildly pathogenic in ducks, but have distinct pathotypes in mice. Moreover, we found that vaccinated poultry in China could be completely protected against H5N8 virus challenge. Given that the H5N8 viruses are likely to continue to spread in wild birds, vaccination of poultry is highly recommended in high-risk countries to prevent H5N8 avian influenza. | Pengfei Cui Xianying Zeng Xuyong Li Yanbing Li Jianzhong Shi Conghui Zhao Zhiyuan Qu Yanwen Wang Jing Guo Wenli Gu Qi Ma Yuancheng Zhang Weipeng Lin Minghui Li Jingman Tian Dongxue Wang Xin Xing Yanjing Liu Shuxin Pan Yaping Zhang Hongmei Bao Liling Liu Guobin Tian Chengjun Li Guohua Deng Hualan Chen | 2022 | Science China(Life Sciences)2022，65，4： | 12 |
| 9 | Chemical conversion coating on AZ31B magnesium alloy and its corrosion tendency显示文摘The morphology change of the magnesium matrix after pre-treatment and the mor- phology as well as the phase composition of chemical conversion coating formed by phosphate were studied using scanning electron microscope and X-ray diffraction. The corrosion resistance of the coating was studied by salt spray and damp test, and the corrosion tendency during salt immersion test was analyzed. The results show that the phase composition before and after pre-treatment is almost change- less, and the deep microflaw appears between α and β phases during acidic pickling. The phosphate conversion coating is mainly composed of Mg, MgO, and some amor- phous phase, and it can provide a good protection for the AZ31B alloy. Results from corrosive morphology indicate that the growth and the corrosion resistance of the phosphate conversion coating are related to the forming process of the AZ31B matrix. | Hualan JIN Xiangjie YANG Ming WANG | 2009 | Acta Metallurgica Sinica(English Letters)2009，22，1： | 7 |
| 10 | Insights from avian influenza surveillance of chickens and ducks before and after exposure to live poultry markets显示文摘Dear Editor, Live poultry markets are the major trading system of poultry in China and many other countries, and are also thought to be important for the generation and spread of avian influenza viruses and other avian pathogens (Zhang et al., 2013;Shi et al., 2017;Quan et al., 2018). | Mei Li Xin Yin Lizheng Guan Xia Zhang Guohua Deng Tao Li Pengfei Cui Yong Ma Yujie Hou Jianzhong Shi Hualan Chen | 2019 | Science China(Life Sciences)2019，62，6： | 6 |
| 11 | TRIM35 mediates protection against influenza infection by activating TRAF3 and degrading viral PB2显示文摘Tripartite motif(TRIM)family proteins are important effectors of innate immunity against viral infections.Here we identified TRIM35 as a regulator of TRAF3 activation.Deficiency in or inhibition of TRIM35 suppressed the production of type I interferon(IFN)in response to viral infection.777m35-deficient mice were more susceptible to influenza A virus(IAV)infection than were wild-type mice.TRIM35 promoted the RIG-Imediated signaling by catalyzing Lys63-linked polyubiquitination of TRAF3 and the subsequent formation of a signaling complex with VISA and TBK1.IAV PB2 polymerase countered the innate antiviral immune response by impeding the Lys63-linked polyubiquitination and activation of TRAF3.TRIM35 mediated Lys48-linked polyubiquitination and proteasomal degradation of IAV PB2,thereby antagonizing its suppression of TRAF3 activation.Our in vitro and in vivo findings thus reveal novel roles of TRIM35,through catalyzing Lys63-or Lys48-linked polyubiquitination,in RIG-I antiviral immunity and mechanism of defense against IAV infection. | Nan Sun Li Jiang Miaomiao Ye Yihan Wang Guangwen Wang Xiaopeng Wan Yuhui Zhao Xia Wen Libin Liang Shujie Ma Liling Liu Zhigao Bu Hualan Chen Chengjun Li | 2020 | Protein & Cell2020，11，12： | 4 |
| 12 | Morphology-Controlled Fabrication of Sulfonated Graphene/Polyaniline Nanocomposites by Liquid/Liquid Interfacial Polymerization and Investigation of their Electrochemical Properties显示文摘新奇控制形态学的策略被开发了制作 sulfonated graphene/polyaniline (SGEP ) 由液体 / 液体的 nanocomposites 界面的聚合。Sulfonated graphene (SGE ) 表被综合并且为 polyaniline (PANI ) 的聚合用作 macromolecular 酸掺杂物和底层,负担得起 SGEP nanocomposites。在 nanocomposites 的 PANI 的形态学能被控制由改变合成条件是 nanorods 或 nanogranules。SGEP 的形态学和 PANI 的形状能被增加另外的掺杂物并且改变使用的 SGE 的数量调节,并且这作为 supercapacitor 电极材料在 nanocomposites 的电气化学的表演上有重要影响。有 PANI nanorods 的 SGEP nanocomposite 在 100 个周期和好率性质以后与 96% 的能力保留展出了 763 F/g 的一个特定的电容。与 SGE 的二不同比率作为另外的酸掺杂物与 HCl 获得到 PANI 的 Composites 分别地在 77% 和 76% 的 100 个周期以后显示出 793 和 931 F/g,而是更低的能力保留的更高特定的电容。 | Qingli Hao Hualan Wang Xujie Yang Lude Lu Xin Wang | 2011 | Nano Research2011，4，4： | 3 |
| 13 | Influenza A virus use of BinCARD1 to facilitate the binding of viral NP to importinα7 is counteracted by TBK1-p62 axis-mediated autophagy显示文摘As a major component of the viral ribonucleoprotein(vRNP)complex in influenza A virus(IAV),nucleoprotein(NP)interacts with isoforms of importinαfamily members,leading to the import of itself and vRNP complex into the nucleus,a process pivotal in the replication cycle of IAV.In this study,we found that BinCARD1,an isoform of Bcl10-interacting protein with CARD(BinCARD),was leveraged by IAV for efficient viral replication.BinCARD1 promoted the nuclear import of the vRNP complex and newly synthesized NP and thus enhanced vRNP complex activity.Moreover,we found that BinCARD1 interacted with NP to promote NP binding to importinα7,an adaptor in the host nuclear import pathway.However,we also found that BinCARD1 promoted RIG-I-mediated innate immune signaling by mediating Lys63-linked polyubiquitination of TRAF3,and that TBK1 appeared to degrade BinCARD1.We showed that BinCARD1 was polyubiquitinated at residue K103 through a Lys63 linkage,which was recognized by the TBK1-p62 axis for autophagic degradation.Overall,our data demonstrate that IAV leverages BinCARD1 as an important host factor that promotes viral replication,and two mechanisms in the host defense system are triggered—innate immune signaling and autophagic degradation—to mitigate the promoting effect of BinCARD1 on the life cycle of IAV. | Xuyuan Wang Li Jiang Guangwen Wang Wenjun Shi Yuzhen Hu Bo Wang Xianying Zeng Guobin Tian Guohua Deng Jianzhong Shi Liling Liu Chengjun Li Hualan Chen | 2022 | Cellular & Molecular Immunology2022，19，10： | 3 |
| 14 | H7N9 virus infection triggers lethal cytokine storm by activating gasdermin E-mediated pyroptosis of lung alveolar epithelial cells显示文摘The H7N9 influenza virus emerged in China in 2013,causing more than 1560 human infections,39% of which were fatal.A ‘cytokine storm’ in the lungs of H7N9 patients has been linked to a poor prognosis and death;however,the underlying mechanism that triggers the cytokine storm is unknown.Here,we found that efficient replication of the H7N9 virus in mouse lungs activates gasdermin E(GSDME)-mediated pyroptosis in alveolar epithelial cells,and that the released cytosolic contents then trigger a cytokine storm.Knockout of Gsdme switched the manner of death of A549 and human primary alveolar epithelial cells from pyroptosis to apoptosis upon H7N9 virus infection,and Gsdme knockout mice survived H7N9 virus lethal infection.Our findings reveal that GSDME activation is a key and unique mechanism for the pulmonary cytokine storm and lethal outcome of H7N9 virus infection and thus opens a new door for the development of antivirals against the H7N9 virus. | Xiaopeng Wan Jiqing Li Yupeng Wang Xiaofei Yu Xijun He Jianzhong Shi Guohua Deng Xianying Zeng Guobin Tian Yanbing Li Yongping Jiang Yuntao Guan Chengjun Li Feng Shao Hualan Chen | 2022 | National Science Review2022，9，1： | 3 |
| 15 | Current Development Situation and Prospects of Blueberry in Guangdong Province显示文摘With high nutrition and health value,blueberry receives much concern in recent years. Blueberry experts in the world are cultivating new blueberry varieties. They have come up with constructive theoretical support and planting technology researches and are constantly devoted to expanding blueberry planting latitudes. This paper elaborated researches of blueberry at home and abroad,current development situation of blueberry in China and Guangdong Province,analyzed strengths of planting blueberry in Guangdong. Besides,it came up with recommendations for development of blueberry industry. Finally,it discussed development prospects of blueberry planting in Guangdong. | Lixia GAO Hualan XIAO Sen LI Fengmin LIU Aiqiong MO | 2015 | Asian Agricultural Research2015，7，9： | 2 |
| 16 | Pandemic threat posed by H3N2 avian influenza virus显示文摘Dear Editor,In the last century,H1N1,H2N2,and H3N2 influenza viruses caused pandemics in 1918,1957,and 1968,respectively.In 2009,a novel H1N1 reassortant jumped from pigs to humans and caused a fourth influenza pandemic.Different lineages of H3N2 influenza viruses are commonly found in animal reservoirs.If a different lineage of H3N2 virus jumps to humans,another human influenza pandemic could occur with devastating consequences. | Yaping Zhang Conghui Zhao Yujie Hou Yan Chen Fei Meng Yichao Zhuang Liling Liu Yasuo Suzuki Jianzhong Shi Guohua Deng Hualan Chen | 2021 | Science China(Life Sciences)2021，64，11： | 2 |
| 17 | Glycosylation and an amino acid insertion in the head of hemagglutinin independently affect the antigenic properties of H5N1 avian influenza viruses显示文摘Antigenic drift forces us to frequently update influenza vaccines; however, the genetic basis for antigenic variation remains largely unknown. In this study, we used clade 7.2 H5 viruses as models to explore the molecular determinants of influenza virus antigenic variation. We generated eight monoclonal antibodies(MAbs) targeted to the hemagglutinin(HA) protein of the index virus A/chicken/Shanxi/2/2006 and found that two representative antigenically drifted clade 7.2 viruses did not react with six of the eight MAbs. The E131 N mutation and insertion of leucine at position 134 in the HA protein of the antigenically drifted strains eliminated the reactivity of the virus with the MAbs. We also found that the amino acid N131 in the H5 HA protein is glycosylated. Our results provide experimental evidence that glycosylation and an amino acid insertion or deletion in HA influence antigenic variation. | Chunyang Gu Xianying Zeng Yangming Song Yanbing Li Liling Liu Yoshihiro Kawaoka Dongming Zhao Hualan Chen | 2019 | Science China(Life Sciences)2019，62，1： | 1 |
| 18 | A nanos- truetured graphene/polyaniline hybrid material for superea- paeitors显示文摘 | WANG Hualan HAO Qingli YANG Xujie | 2010 | Nanoscale2010，2，10： | 1 |
| 19 | Effect of graphene oxide on the properties of its composite with polyaniline显示文摘 | Wang Hualan Hao Qingli Yang Xujie | 2010 | Appl Mater Inter2010，2，3： | 1 |
| 20 | Generation and evaluation of a high-growth reassortant H9N2 influenza A virus as a pandemic vaccine candidate显示文摘 | Hualan chen Kanta subbarao David Swayne | 2003 | Vaccine2003，，21： | 1 |
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