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| 1 | Scutellarin protects oxygen/glucose-deprived astrocytes and reduces focal cerebral ischemic injury显示文摘Scutellarin, a bioactive flavone isolated from Scutellaria baicalensis, has anti-inflammatory, anti-neurotoxic, anti-apoptotic and anti-oxidative effects and has been used to treat cardiovascular and cerebrovascular diseases in China. However, the mechanisms by which scutellarin mediates neuroprotection in cerebral ischemia remain unclear. The interaction between scutellarin and nicotinamide adenine dinucleotide phosphate oxidase 2(NOX2) was assessed by molecular docking study, which showed that scutellarin selectively binds to NOX2 with high affinity. Cultures of primary astrocytes isolated from the cerebral cortex of neonatal Sprague-Dawley rats were pretreated with 2, 10 or 50 μM scutellarin for 30 minutes. The astrocytes were then subjected to oxygen/glucose deprivation by incubation for 2 hours in glucose-free Dulbecco's modified Eagle's medium in a 95% N2/5% CO_2 incubator, followed by simulated reperfusion for 22 hours. Cell viability was assessed by cell counting kit-8 assay. Expression levels of NOX2, connexin 43 and caspase-3 were assessed by western blot assay. Reactive oxygen species were measured spectrophotometrically. Pretreatment with 10 or 50 μM scutellarin substantially increased viability, reduced the expression of NOX2 and caspase-3, increased the expression of connexin 43, and diminished the levels of reactive oxygen species in astrocytes subjected to ischemia-reperfusion. We also assessed the effects of scutellarin in vivo in the rat transient middle cerebral artery occlusion model of cerebral ischemia-reperfusion injury. Rats were given intraperitoneal injection of 100 mg/kg scutellarin 2 hours before surgery. The Bederson scale was used to assess neurological deficit, and 2,3,5-triphenyltetrazolium chloride staining was used to measure infarct size. Western blot assay was used to assess expression of NOX2 and connexin 43 in brain tissue. Enzyme-linked immunosorbent assay was used to detect 8-hydroxydeoxyguanosine(8-OHd G), 4-hydroxy-2-nonenal(4-HNE) and 3-nitrotyrosin(3-NT) in brain tissue. Immunofluorescence double staining was used to determine the co-expression of caspase-3 and Neu N. Pretreatment with scutellarin improved the neurological function of rats with focal cerebral ischemia, reduced infarct size, diminished the expression of NOX2, reduced levels of 8-OHd G, 4-HNE and 3-NT, and reduced the number of cells co-expressing caspase-3 and Neu N in the injured brain tissue. Furthermore, we examined the effect of the NOX2 inhibitor apocynin. Apocynin substantially increased connexin 43 expression in vivo and in vitro. Collectively, our findings suggest that scutellarin protects against ischemic injury in vitro and in vivo by downregulating NOX2, upregulating connexin 43, decreasing oxidative damage, and reducing apoptotic cell death. | Jing-Bo Sun Yan Li Ye-Feng Cai Yan Huang Shu Liu Patrick KK Yeung Min-Zhen Deng Guang-Shun Sun Prince LM Zilundu Qian-Sheng Hu Rui-Xin An Li-Hua Zhou Li-Xin Wang Xiao Cheng | 2018 | Neural Regeneration Research2018，13，8： | 16 |
| 2 | Glucocorticoids upregulate transepithelial electrical resistance and expression of tight junction-related protein in human trabecular meshwork cells显示文摘 | ZHUO Ye-hong, HUANG Ya-lin, WEI Yan-tao, LING Yun-lan, LIN Ming-kai and GE Jian Key Laboratory of Ophthalmology, Ministry of Education Zhongshan Ophtha lm ic Center , Sun Yat-sen University, Guangzhou 510060, China | 2005 | Chinese Medical Journal2005，，20： | 4 |
| 3 | Partitioning regularity of nonionic organic mixtures in organic phase/water system显示文摘The partitioning regularity of nonionic organic mixtures in organic phase/water system is revealed. The equation for calculating the partition coefficients of mixtures (KMD), together with the determination model, is derived from the equilibrium partitioning models (EPMs). Based on these derived equations, the KMD values of 20 mixtures containing halogenated benzenes are obtained. The results show that stronger hydrophobidty of an individual chemical in the mixture results in the stronger hydrophobidty of the mixture and the greater the proportion of this chemical, the stronger the hydrophobidty of the mixture will be. This partitioning regularity is helpful to the study of the toxicity for mixtures and the environmental behavior, such as transfer or accumulation, for mixed organic pollutants. | Lin, ZF Yu, HX Huang, LM Feng, JF Wang, LS | 2001 | Chinese Science Bulletin2001，46，17： | 2 |
| 4 | Effet of Maotai liguor in inducing metallothioneins and on hepatic stellate cells显示文摘 | Cheng ML Wu J Wang HQ Xue LM Tan YZ Liu P Li CX Huang NH Yao YM Ren LZ Ye L Li L Jia ML | 2001 | Shijie Huaren Xiaohua Zazhi2001，9，12： | 2 |
| 5 | A role for cofactor-cofactor and cofac-tor-histone interactions in targeting p300,SWI/SNF and mediator for transcription显示文摘 | Huang ZQ Li J Sachs LM | | 0，，： | 1 |
| 6 | Varicella break through infection and vaccine effectiveness in Taiwan 显示文摘 | Huang WC Huang LM Chang I | 2011 | Vaccine2011，29，15： | 1 |
| 7 | The role of tyrosine kinase Etk/Bmx in EGF-induced apoptosis of MDA-MB-468 breast cancer cells 显示文摘 | Chen KY Huang LM Kung HJ | 2004 | Oncogene2004，23，10： | 1 |
| 8 | Structural comparisons of class I phosphoinositide 3-kinases 显示文摘 | Amzel LM Huang CH Mandelker D | 2008 | Nat Rev Cancer2008，8，9： | 1 |
| 9 | Plasma adiponectin levels and blood pressures in nondiabetic adolescent females显示文摘 | Huang KC Chen CL Chuang LM | 2003 | J Clin Endocrinol Metab2003，88，9： | 1 |
| 10 | Reciprocal regulatory interaction between human herpesvirus 8 and human immunodeficiency virus type 1显示文摘 | Huang LM Chao MF Chen MY | 2001 | J Biol Chem2001，276，13： | 1 |
| 11 | Clinical staging and managing strategy of hand foot and mouth disease 显示文摘 | Zhu QR Huang LM Yang SD | 2009 | Chin J Evid Based Pedaitr2009，4，3： | 1 |
| 12 | Therapeutically relevant concentrations of raloxifene dilate pressurized rat resistance arteries via calcium-dependent endothelial nitric oxide synthase activation显示文摘 | Chan YC Leung FP Wong WT Tian XY Yung LM Lau CW Tsang SY Yao X Chen ZY Huang Y | | 0，，： | 1 |
| 13 | An outbreak of coxsackievirus A16 infection: comparison with other enteroviruses in a preschool in Taipei显示文摘 | Wu PC Huang LM Kao CL | 2010 | J Microbiol Immunol Infect2010，43，4： | 1 |
| 14 | Invasive fungal infection laboratory diagnosis and antifungal treatment显示文摘 | Shao PL Huang LM Hsuen PR | 2006 | J Microbiol Immunol Infect2006，19，： | 1 |
| 15 | Imaging characteristics of neovascular pigment epithelial detachments and their response to anti-vascular endothelial growth factor therapy 显示文摘 | Punjabi as Huang J Rodriguez L Lyon AT Jampol LM Mirza RG | 2013 | Br J Ophthalmol2013，97，8： | 1 |
| 16 | In vitro biodegradation of three brushite calcium phosphate cements by a macrophage cell-line 显示文摘 | X ia Z Grovcr LM Huang Y | 2006 | Biomaterials2006，27，26： | 1 |
| 17 | Neurodevelopment and cog- nition in children after enterovirus 71 infeetion显示文摘 | Chang LY Huang LM Gau SS | 2007 | N Engl J Med2007，356，12： | 1 |
| 18 | Interleukin 27 negatively regulates the development of interleukin 17-producing T helper cells during chronic inflammation of the central nervous system 显示文摘 | Stumhofer JS Laurence A Wilson EH Huang E Tato CM Johnson LM | 2006 | Nat Immunol2006，7，9： | 1 |
| 19 | Clinical features and factors of unfavorable outcomes for non-polio enterovirus in- fection of the centeral nervous system in northern Taiwan, 1994- 2003 显示文摘 | Yang TT Huang LM Lu CY | 2005 | J MicrobiolImmunol Infect2005，38，6： | 1 |
| 20 | Notchl modulates timing of G1-S progression by inducing SKP2 transcription and p27 Kipl degradation 显示文摘 | Sarmento LM Huang H Limon A | 2005 | J Exp Med2005，202，1： | 1 |
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