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| 1 | Identification of a novel coronavirus causing severe pneumonia in human:a descriptive study显示文摘Background:Human infections with zoonotic coronaviruses(CoVs),including severe acute respiratory syndrome(SARS)-CoV and Middle East respiratory syndrome(MERS)-CoV,have raised great public health concern globally.Here,we report a novel batorigin CoV causing severe and fatal pneumonia in humans.Methods:We collected clinical data and bronchoalveolar lavage(BAL)specimens from five patients with severe pneumonia from Wuhan Jinyintan Hospital,Hubei province,China.Nucleic acids of the BAL were extracted and subjected to next-generation sequencing.Virus isolation was carried out,and maximum-likelihood phylogenetic trees were constructed.Results:Five patients hospitalized from December 18 to December 29,2019 presented with fever,cough,and dyspnea accompanied by complications of acute respiratory distress syndrome.Chest radiography revealed diffuse opacities and consolidation.One of these patients died.Sequence results revealed the presence of a previously unknownβ-CoV strain in all five patients,with 99.8%to 99.9%nucleotide identities among the isolates.These isolates showed 79.0%nucleotide identity with the sequence of SARS-CoV(GenBank NC_004718)and 51.8%identity with the sequence of MERS-CoV(GenBank NC_019843).The virus is phylogenetically closest to a bat SARS-like CoV(SL-ZC45,GenBank MG772933)with 87.6%to 87.7%nucleotide identity,but is in a separate clade.Moreover,these viruses have a single intact open reading frame gene 8,as a further indicator of bat-origin CoVs.However,the amino acid sequence of the tentative receptor-binding domain resembles that of SARS-CoV,indicating that these viruses might use the same receptor.Conclusion:A novel bat-borne CoV was identified that is associated with severe and fatal respiratory disease in humans. | Li-Li Ren Ye-Ming Wang Zhi-Qiang Wu Zi-Chun Xiang Li Guo Teng Xu Yong-Zhong Jiang Yan Xiong Yong-Jun Li Xing-Wang Li Hui Li Guo-Hui Fan Xiao-Ying Gu Yan Xiao Hong Gao Jiu-Yang Xu Fan Yang Xin-Ming Wang Chao Wu Lan Chen Yi-Wei Liu Bo Liu Jian Yang Xiao-Rui Wang Jie Dong Li Li Chao-Lin Huang Jian-Ping Zhao Yi Hu Zhen-Shun Cheng Un-Lin Liu Zhao-Hui Qian Chuan Qin Qi Jin Bin Cao Jian-Wei Wang | 2020 | Chinese Medical Journal2020，，9： | 99 |
| 2 | Prevalence of Autism Spectrum Disorder in China:A Nationwide Multi-center Population-based Study Among Children Aged 6 to 12 Years显示文摘This study aimed to obtain the first national estimate of the prevalence of autism spectrum disorder(ASD) in Chinese children.We targeted the population of 6 to 12-year-old children for this prevalence study by multistage convenient cluster sampling.The Modified Chinese Autism Spectrum Rating Scale was used for the screening process.Of the target population of 142,086 children,88.5%(n=125,806) participated in the study.A total of 363 children were confirmed as having ASD.The observed ASD prevalence rate was 0.29%(95% CI:0.26%-0.32%) for the overall population.After adjustment for response rates,the estimated number of ASD cases was867 in the target population sample,thereby achieving an estimated prevalence of 0.70%(95% CI:0.64%-0.74%).The prevalence was significantly higher in boys than in girls(0.95%;95% CI:0.87%-1.02% versus 0.30%;95%CI:0.26%-0.34%;P <0.001).Of the 363 confirmed ASD cases,43.3% were newly diagnosed,and most of those(90.4%) were attending regular schools,and 68.8% of the children with ASD had at least one neuropsychiatric comorbidity.Our findings provide reliable data on the estimated ASD prevalence and comorbidities in Chinese children. | Hao Zhou Xiu Xu Weili Yan Xiaobing Zou Lijie Wu Xuerong Luo Tingyu Li Yi Huang Hongyan Guan Xiang Chen Meng Mao Kun Xia Lan Zhang Erzhen Li Xiaoling Ge Lili Zhang Chunpei Li Xudong Zhang Yuanfeng Zhou Ding Ding Andy Shih Eric Fombonne Yi Zheng Jisheng Han Zhongsheng Sun Yong-hui Jiang Yi Wang LATENT-NHC Study Team | 2020 | Neuroscience Bulletin2020，36，9： | 158 |
| 3 | NLRP3 inflammasome activation and cell death显示文摘The NLRP3 inflammasome is a cytosolic multiprotein complex composed of the innate immune receptor protein NLRP3,adapter protein ASC,and inflammatory protease caspase-1 that responds to microbial infection,endogenous danger signals,and environmental stimuli.The assembled NLRP3 inflammasome can activate the protease caspase‐1 to induce gasdermin D-dependent pyroptosis and facilitate the release of IL-1β and IL-18,which contribute to innate immune defense and homeostatic maintenance.However,aberrant activation of the NLRP3 inflammasome is associated with the pathogenesis of various inflammatory diseases,such as diabetes,cancer,and Alzheimer’s disease.Recent studies have revealed that NLRP3 inflammasome activation contributes to not only pyroptosis but also other types of cell death,including apoptosis,necroptosis,and ferroptosis.In addition,various effectors of cell death have been reported to regulate NLRP3 inflammasome activation,suggesting that cell death is closely related to NLRP3 inflammasome activation.In this review,we summarize the inextricable link between NLRP3 inflammasome activation and cell death and discuss potential therapeutics that target cell death effectors in NLRP3 inflammasome-associated diseases. | Yi Huang Wen Xu Rongbin Zhou | 2021 | Cellular & Molecular Immunology2021，18，9： | 94 |
| 4 | Analysis and design for the second order nonlinear continuous extended states observer显示文摘The extended state observer (ESO) is a novel observer for a class of uncertain systems. Since ESO adopts the continuous non-smooth structure, the classical observer design theory is hard to use for ESO analysis. In this note, the self-stable region (SSR) approach, which is a nonlinear synthesis method for nonlinear uncertain systems, will be used for ESO design and its stability analysis. The advantages of the non-smooth structure in ESO for improving the convergence properties and the estimation precision will be shown. | Yi Huang Jingqing Han | 2000 | Chinese Science Bulletin2000，45，21： | 65 |
| 5 | Remediation of Heavy Metal-Polluted Agricultural Soils Using Clay Minerals: A Review显示文摘Heavy metal contamination of agricultural soils poses risks and hazards to humans. The remediation of heavy metal-polluted soils has become a hot topic in environmental science and engineering. In this review, the application of clay minerals for the remediation of heavy metal-polluted agricultural soils is summarized, in terms of their remediation effects and mechanisms, influencing factors,and future focus. Typical clay minerals, natural sepiolite, palygorskite, and bentonite, have been widely utilized for the in-situ immobilization of heavy metals in soils, especially Cd-polluted paddy soils and wastewater-irrigated farmland soils. Clay minerals are able to increase soil p H, decrease the chemical-extractable fractions and bioavailability of heavy metals in soils, and reduce the heavy metal contents in edible parts of plants. The immobilization effects have been confirmed in field-scale demonstrations and pot trials. Clay minerals can improve the environmental quality of soils and alleviate the hazards of heavy metals to plants. As main factors affecting the immobilization effects, the p H and water condition of soils have drawn academic attention. The remediation mechanisms mainly include liming, precipitation, and sorption effects. However, the molecular mechanisms of microscopic immobilization are unclear. Future studies should focus on the long-term stability and improvement of clay minerals in order to obtain a better remediation effect. | XU Yi LIANG Xuefeng XU Yingming QIN Xu HUANG Qingqing WANG Lin SUN Yuebing | 2017 | Pedosphere2017，27，2： | 66 |
| 6 | 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents显示文摘Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department visits.Recurrent syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in China, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese Medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese Medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese College of Cardiovascular Physicians, Chinese Medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing Medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up. | Cheng Wang Yaqi Li Ying Liao Hong Tian Min Huang Xiangyu Dong Lin Shi Jinghui Sun Hongfang Jin Junbao Du Jindou An Jie Chen Mingwu Chen Qi Chen Sun Chen Yonghong Chen Zhi Chen Adolphus Kai-tung Chau Junbao Du Zhongdong Du Junkai Duan Hongyu Duan Xiangyu Dong Lin Feng Lijun Fu Fangqi Gong Yonghao Gui Ling Han Zhenhui Han Bing He Zhixu He Xiufen Hu Yimin Hua Guoying Huang Min Huang Ping Huang Yujuan Huang Hongfang Jin Mei Jin Bo Li Fen Li Tao Li Xiaohui Li Xiaoyan Liu Yan Li Haitao Lv Tiewei Lv Zipu Li Luyi Ma Silin Pan Yusheng Pang Hua Peng Yuming Qin Jie Shen Lin Shi Kun Sun Jinghui Sun Hong Tian Jie Tian Cheng Wang Hong Wang Lei Wang Jinju Wang Wendi Wang Yuli Wang Rongzhou Wu Tianhe Xia Yanyan Xiao Chunhong Xie Yanlin Xing Zhenyu Xiong Baoyuan Xu Yi Xu Hui Yan Shiwei Yang Qijian Yi Xia Yu Xianyi Yu Yue Yuan Hongyan Zhang Huili Zhang Li Zhang Qingyou Zhang Xi Zhang Yanmin Zhang Zhiwei Zhang Cuifen Zhao Bin Zhou Hua Zhu | 2018 | Science Bulletin2018，63，23： | 54 |
| 7 | Activation of gibberellin 2-oxidase 6 decreases active gibberellin levels and creates a dominant semi-dwarf phenotype in rice (Oryza sativa L.)显示文摘Gibberellin (GA) 2-oxidase plays a key role in the GA catabolic pathway through 2β-hydroxylation.In the present study,we isolated a CaMV 35S-enhancer activation tagged mutant,H032.This mutant exhibited a dominant dwarf and GA-deficient phenotype,with a final stature that was less than half of its wild-type counterpart.The endogenous bioactive GAs are markedly decreased in the H032 mutant,and application of bioactive GAs (GA3 or GA4) can reverse the dwarf phenotype.The integrated T-DNA was detected 12.8 kb upstream of the OsGA2ox6 in the H032 genome by TAIL-PCR.An increased level of OsGA2ox6 mRNA was detected at a high level in the H032 mutant,which might be due to the enhancer role of the CaMV 35S promoter.RNAi and ectopic expression analysis of OsGA2ox6 indicated that the dwarf trait and the decreased levels of bioactive GAs in the H032 mutant were a result of the up-regulation of the OsGA2ox6 gene.BLASTP analysis revealed that OsGA2ox6 belongs to the class III of GA 2-oxidases,which is a novel type of GA2ox that uses C20-GAs (GA12 and/or GA53) as the substrates.Interestingly,we found that a GA biosynthesis inhibitor,paclobutrazol,positively regulated the OsGA2ox6 gene.Unlike the over-expression of OsGA2ox1,which led to a high rate of seed abortion,the H032 mutant retained normal flowering and seed production.These results indicate that OsGA2ox6 mainly affects plant stature,and the dominant dwarf trait of the H032 mutant can be used as an efficient dwarf resource in rice breeding. | Jian Huang Ding Tang Yi Shen Baoxiang Qin Lilan Hong Aiqing You Ming Li Xin Wang Hengxiu Yu Minghong Gu Zhukuan Cheng | 2010 | Journal of Genetics and Genomics2010，37，1： | 36 |
| 8 | Prevalence estimates for primary brain tumors in China: a multi-center cross-sectional study显示文摘背景尽管在中国的死亡的第一个领先的原因是恶意的瘤(死亡, 374.1 每 100000 人年),因为关于学习试图在 PBT.Methods 的流行上报导一个全面评价的大脑 tumors.This 的流行病的特征有一些很好记录的报告,保健系统上的主要大脑肿瘤( PBT )的完整的影响完全没被描述 multicenter 大脑肿瘤( MCSBT )上的代表性的学习在里面中国在五地区性的 c 被开始59 岁;女性: 45 岁) .Conclusions 年龄标准化了 PBT 的流行是 22.52 每 100 000 (95% CI , 13.22~31.82 )为所有人口, 17.64 每 100 000 (95% CI , 9.41~25.87 )为人,和 27.94 同等 100 000 (95% CI , 17.58~38.30 )为有 PBT.Our 流行的 304 954 个案例的一张估计的人口估计的到中国产出的 2010 人口的 women.Age 标准化提供计划护理服务并且为 surviving.In 开发编程的策略的一个保守基础未来,它将是 | JIANG Tao TANG Gen-fu LIN Yi PENG Xiao-xia ZHANG Xiao ZHAI Xiu-wei PENG Xiang YANG Jin-qing HUANG Hong-er WU Nai-feng CHEN Xiao-jun XING Hou-xun SU Tong-yong WANG Zhong-cheng | 2011 | Chinese Medical Journal2011，，17： | 40 |
| 9 | Graphene-Based Conducting Inks for Direct Inkjet Printing of Flexible Conductive Patterns and Their Applications in Electric Circuits and Chemical Sensors显示文摘打印过程的一系列 inkjet 用基于 graphene 的墨水被学习了。在优化条件下面,使用水溶性的单人赛分层的 graphene 氧化物(去)并且很少分层的 graphene 氧化物( FGO ),各种各样的高图象质量模式能在多样的灵活底层上被打印包括纸, poly (乙烯 terephthalate )(宠物)并且 polyimide ( PI ),与打印技术的简单、便宜的 inkjet 。在塑料底层上打印的基于 graphene 的模式在热减小以后表明了高电的传导性,并且更重要地,他们在严重弯曲周期上保留了一样的传导性。因此,灵活电的电路和一个氢过氧化物化学药品传感器被制作并且显示出优秀表演,演示打印用 graphene 墨水的技术的这简单、实际的 inkjet 的应用程序。结果显示出那 graphene 材料 -- 它能容易在大规模上被生产并且为灵活、便宜的基于 graphene 的电子设备的方便制造拥有突出的电子性质有伟人潜力,由使用打印技术的简单 inkjet。 | Lu Huang Yi Huang diajie Liang Xiangjian Wan Yongsheng Chen | 2011 | Nano Research2011，4，7： | 31 |
| 10 | Arabidopsis TT19 Functions as a Carrier to Transport Anthocyanin from the Cytosol to Tonoplasts显示文摘Anthocyanins 在 endoplasmic 蜂窝胃的 cytosolic 表面被综合(嗯) 但是主导地在液泡积累。很少 anthocyanins 怎么从 ER 被搬运到液泡被知道。这里,我们提供支持那张透明种皮 19 的证据(TT19 ) ,谷胱甘肽 S-transferase (GST ) ,作为一个搬运人工作把 cyanidin 或 anthocyanins 搬运到 tonoplast。我们识别了新奇 tt19 异种(tt19-7 ) ,它恰好积累 anthocyanins,但是比的生产黄酮醇的 36% 高水平野类型(WT ) ,从乙醇 methanesulfonate mutagenized 种子。在 tt19-7 表示熔化 TT19 的绿荧光蛋白质(GFP ) 在有缺点的 anthocyanin 生合成救变异的显型,显示 TT19GFP 是功能的。我们进一步证明 TT19GFP 在细胞质和原子核是局部性的不仅,而且在 tonoplast 上。TT19GFP 的膜本地化被 immunoblot 分析进一步查明。在 vitro,试金证明净化的 recombinant TT19 增加浇 cyanidin (Cya ) 和 cyanidin-3-O-glycoside (C3G ) 的溶解度。与 C3G 相比, Cya 能戏剧性地熄灭 TT19 的内在的色氨酸荧光到许多底层,显示到比到 C3G 的 Cya 的 TT19 的一种更高的亲密关系。等温的滴定热量测定分析也证实了在 TT19 和 C3G 之间的物理相互作用。一起拿,我们的数据揭示分子的机制位于 \O 下面调停 TT19 的 anthocyanin 交通。 | Yi Sun Hong Li Ji-Rong Huang | 2012 | Molecular Plant2012，5，2： | 31 |
| 11 | Simultaneous Fe(Ⅲ) reduction and ammonia oxidation process in Anammox sludge显示文摘In recent years, there have been a number of reports on the phenomenon in which ferric iron(Fe(Ⅲ)) is reduced to ferrous iron [Fe(Ⅱ)] in anaerobic environments, accompanied by simultaneous oxidation of ammonia to NO2-, NO3-, or N2.However, studies on the relevant reaction characteristics and mechanisms are rare. Recently, in research on the effect of Fe(Ⅲ) on the activity of Anammox sludge, excess ammonia oxidization has also been found.Hence, in the present study, Fe(Ⅲ) was used to serve as the electron acceptor instead of NO2-,and the feasibility and characteristics of Anammox coupled to Fe(Ⅲ) reduction(termed Feammox) were investigated. After 160 days of cultivation, the conversion rate of ammonia in the reactor was above 80%, accompanied by the production of a large amount of NO3-and a small amount of NO2-. The total nitrogen removal rate was up to 71.8%. Furthermore,quantities of Fe(Ⅱ) were detected in the sludge fluorescence in situ hybridization(FISH) and denaturated gradient gel electrophoresis(DGGE) analyses further revealed that in the sludge, some Anammox bacteria were retained, and some microbes were enriched during the acclimatization process. We thus deduced that in Anammox sludge, Fe(Ⅲ) reduction takes place together with ammonia oxidation to NO2-and NO3-along with the Anammox process. | Xiang Li Yong Huang Heng-wei Liu Chuan Wu Wei Bi Yi Yuan Xin Liu | 2018 | Journal of Environmental Sciences2018，30，2： | 30 |
| 12 | Induction of the LRP16 gene by estrogen promotes the invasive growth of Ishikawa human endometrial cancer cells through the downregulation of E-cadherin显示文摘LRP16 以前在乳癌房间作为导致雌激素的基因被识别。到在子宫内膜的癌症(EC ) 的雌激素和它的功能的效果的 LRP16 的应答的海角房间仍然是不清楚的。这里,我们证明 LRP16 基因的信使 rna 水平和倡导者活动被 17beta-estradiol (E2 ) 显著地在雌激素受体高山增加哈(嗯高山哈)-positiveIshikawa 人 EC 房间。尽管 Ishikawa 细胞的生长率没被 LRP16 的宫外的表示显然影响, Transwell 试金的结果显示出 LRP16-overexpressing 细胞的侵略能力的近似 one-thirdincrease。由于分子的屏蔽,我们观察到 E-cadherin 的表示,与肿瘤转移联系的一个必要粘附分子,被 LRP16 镇压。进一步的倡导者分析以一种剂量依赖者方式表明了那 LRP16 inhibitedE-cadherin transactivation。然而,抑制被雌激素剥夺废除,显示由 LRP16requires 的 E-cadherin 抄写的 down 规定嗯高山哈调停。染色质免疫降水分析表明到 E-cadherin 倡导者的 ERalpha 的绑定被 LRP16 反对,建议那 LRP16 能防碍嗯调停 alpha 的抄写。这些结果建议起来由雌激素的 LRP16 的规定能被在人的 EC 调整 E-cadherin 的 down 涉及侵略生长。 | Yuan Guang Meng Wei Dong Han Ya Li Zhao Ke Huang Yi Ling Si Zhi Qiang Wu Yi Ming Mu | 2007 | Cell Research2007，17，10： | 27 |
| 13 | Sufentanil reduces emergence agitation in children receiving sevoflurane anesthesia for adenotonsillectomy compared with fentanyl显示文摘背景出现焦虑是在小儿科的麻醉的一个普通问题,特别在 sevoflurane 正式就职和这研究的维护 anesthesia.The 目的是调查在与 fentanyl.Methods 相比经历 adenotonsillectomy 的孩子在 sevoflurane 麻醉以后减少出现焦虑的 sufentanil 的效果以后 105 个孩子 3-11 年,随机被分配收到正常盐(控制组), sufentanil 0.2 g/kg ( S2 )或芬太奴 2 g/kg ( F2 ) 1 | LI Jun HUANG Zhi-lian ZHANG Xu-tong LUO Ke ZHANG Zhan-qin MAO Yi ZHUANG Xiao-biao LIAN Qing-quan CAO Hong | 2011 | Chinese Medical Journal2011，，22： | 27 |
| 14 | Postoperative adjuvant antiviral therapy for hepatitis B/C virus-related hepatocellular carcinoma:A meta-analysis显示文摘AIM:To investigate the impact of postoperative antiviral treatment on tumor recurrence and survival of patients with chronic hepatitis B virus(HBV) or hepatitis C virus(HCV) infection-related primary hepatocellular carcinoma(HCC) after curative therapy.METHODS:We performed a meta-analysis of randomized and non-randomized control trials from electronic search and manual search.The fixed effect model of Mantel-Haenszel method and the random effect model of Der Simonian and Laird method were used for homogeneous and heterogeneous studies,respectively.Seven HCV-related studies,three HBV-related studies and three studies on HBV or HCV-related HCC were identified.RESULTS:A total of 1224 patients were included in this analysis.The estimated odds ratios(OR) for the 1-,2-,3-and 5-year recurrence were 0.54 [15.4% vs 24.1%,95% confidence interval(CI):0.32-0.89,P=0.02],0.42(36.9% vs 58.0%,95% CI:0.19-0.90,P=0.03),0.37(47.9% vs 63.8%,95% CI:0.19-0.71,P=0.003),and 0.32(66.7% vs 74.3%,95% CI:0.15-0.66,P=0.002),respectively;and the OR for the 1-,2-,3-,5-and 7-year mortality were 0.23(1.2% vs 9.1%,95% CI:0.07-0.71,P=0.01),0.31(6.4% vs 22.1%,95% CI:0.12-0.79,P=0.01),0.43(12.7% vs 20.8%,95% CI:0.21-0.89,P=0.02),0.42(25.1% vs 42.0%,95% CI:0.27-0.66,P=0.0002) and 0.28(31.9% vs 52.2%,95% CI:0.13-0.59,P=0.0008).CONCLUSION:This meta-analysis indicates the postoperative antiviral therapy,interferon in particular,may serve as a favorable alternative to reduce recurrence and mortality in patients with HBV/HCV related HCCs. | Miao, Ruo-Yu Zhao, Hai-Tao Yang, Hua-Yu Mao, Yi-Lei Lu, Xin Zhao, Yi Liu, Chang-Ning Zhong, Shou-Xian Sang, Xin-Ting Huang, Jie-Fu | 2010 | World Journal of Gastroenterology2010，16，23： | 26 |
| 15 | Expression of miR-125b in the new,highly invasive glioma stem cell and progenitor cell line SU3显示文摘MicroRNA (miR)-125b has been shown to play a potential role in the development of glioma stem cells.However,the relationship between miRNA and glioma stem cells is still elusive.This study was designed to elucidate this potential relationship.We established a highly invasive glioma stem cell and progenitor (GSCP) cell line SU3.SU3 cell suspensions were injected into nude mice brains in situ,and the invasiveness of graft tumors was analyzed using hematoxylin and eosin staining as well as immunohistochemistry.Real-time polymerase chain reaction (PCR) was used to measure the expression levels of miR-125b in SU3 and other cells.In vitro,SU3 cells expressed CD133 and nestin as well as differentiation markers glial fibrillary acidic protein (GFAP) and β-tubulin III,which were consistent with the characteristics of glioma stem cells.Scratch assays indicated that the migration ability of SU3 cells was stronger than that of U251 stem cells (U251s).In vivo,SU3 cells invaded into each part of the mouse brain from the caudate nucleus in a diffuse pattern and highly expressed invasive and proliferative cell markers matrix metalloprotease 2 (MMP2),MMP9,and Ki-67.Real-time PCR results revealed that the levels of miR-125b and MMP9 were significantly higher in SU3 and SU2,also a highly invasive GSCP cell line we established before,than in U251s.High expression of miR-125b both in newly established GSCPs,SU3,and long-term cultured GSCPs,SU2 suggests that miR-125b exhibits oncogene-like behavior.This behavior should be considered in further studies of miR-125b in cancer stem cells.Furthermore,MMP9,which plays a role in cancer stem cell invasion,may be a target gene of miR-125b. | Yi Wan Xi-Feng Fei Zhi-Min Wang Dong-Yi Jiang Han-Chun Chen Jian Yang Lei Shi Qiang Huang | 2012 | Chinese Journal of Cancer2012，31，4： | 25 |
| 16 | Requirement and Functional Redundancy of Ib Subgroup bHLH Proteins for Iron Deficiency Responses and Uptake in Arabidopsis thaliana显示文摘在 Arabidopsis 的 bHLH 基因家庭的 Ib 亚群包含四个成员(AtbHLH38, AtbHLH39, AtbHLH100,和 AtbHLH101 ) 。AtbHLH38 和 AtbHLH39 以前被证实与象 FER 一样铁缺乏交往导致的抄写因素(合适) ,直接在 ferricchelate reductase FRO2 和高亲密关系的铁的铁 transporter IRT1 的表示的激活工作。在这个工作,我们在铁缺乏回答和举起的规定描绘了 AtbHLH100 和 AtbHLH101 的功能。酵母二混血儿的分析和双分子的荧光互补试金证明 AtbHLH100 和 AtbHLH101 能交往与合适。AtbHLH100 或 AtbHLH101 与的双表示装入激活的酵母房间 FRO2 和 IRT1 的倡导者驾驶的 GUS 表示。工厂 overexpressing 与 AtbHLH101 结合了在根显示出 FRO2 和 IRT1 的组成的表示,并且在射击积累了更多的铁。进一步, AtbHLH38, AtbHLH39, AtbHLH100,和 AtbHLH101 的单个、双、三倍的猛烈异种被产生并且描绘。在根的 FRO2 和 IRT1 表示和在射击的铁内容更急速地比双的可得到的其它和四基因的三倍的异种的在 AtbHLH39, AtbHLH100,和 AtbHLH101 的三倍的猛烈异种被减少。象在在铁缺乏下面的多重猛烈异种的 FRO2 和 IRT1 的表示一样的生理的回答的比较表明 AtbHLH100, AtbHLH38, AtbHLH101,和 AtbHLH39 在铁缺乏回答和举起起了逐渐地增加的重要作用。一起拿所有,我们断定四 Ib 亚群 bHLH 蛋白质在 Arabidopsis 为铁缺乏回答和举起的激活与微分意义被要求并且拥有冗余的功能。 | Ning Wang Yan Cui Yi Liu Huajie Fan Juan Du Zongan Huang Youxi Yuan Huilan Wu Hong-Qing Ling | 2013 | Molecular Plant2013，6，2： | 26 |
| 17 | Prevalence of Thyroid Nodules and Its Relationship with Iodine Status in Shanghai:a Population-based Study显示文摘Objective This study was designed to evaluate the prevalence of thyroid nodules(TNs) and its relationship with urine iodine concentrations(UICs) after the regional rapid economic growth and lifestyle changes. Methods A cross-sectional survey was conducted in the general population aged 15-69 years. A questionnaire regarding general and personal characteristics and relevant information was administered. Ultrasonography of the thyroid was performed, and serum triiodothyronine(T3), tetraiodothyronine(T4), serum thyroid stimulating hormone(TSH), free triiodothyronine(FT3), free tetraiodothyronine(FT4), thyroglobulin antibody(Tg Ab), thyroid peroxidase antibody(TPOAb), and TSH receptor antibody(TRAb) levels were measured for each individual subject. Results The prevalence rates of TNs in the whole population, females and males were 27.76%, 34.04%, and 21.60%, respectively. The prevalence of multiple nodules increased with age, whereas the prevalence peaks differed between males and females. The median UICs in the whole population and females with non-TNs were higher than those of subjects with TNs(P=0.0035, P=0.0068). The median UICs in subjects with a single TN were higher than those in subjects with multiple TNs(P=0.0164, P=0.0127). The result showed a U-shaped curve relationship between UIC and prevalence of TNs. The prevalence of TNs was the lowest when the UIC was 140-400 μg/L. Conclusion The prevalence of TNs was nearly 30% and increased with age. The relationship between UIC and prevalence of TNs is U-shaped, with an increase in risk when the UIC was <140 μg/L and >400 μg/L. Very low or high UIC levels need attention and correction. | SONG Jun ZOU Shu Rong GUO Chang Yi ZANG Jia Jie ZHU Zhen Ni MI Ming HUANG Cui Hua YU Hui Ting LU Xi RUAN Ye WU Fan | 2016 | Biomedical and Environmental Sciences2016，29，6： | 25 |
| 18 | Nomogram to predict overall survival after gallbladder cancer resection in China显示文摘AIM To integrate clinically significant variables related to prognosis after curative resection for gallbladder carcinoma(GBC) into a predictive nomogram.METHODS One hundred and forty-two GBC patients who underwent curative intent surgical resection at Peking Union Medical College Hospital(PUMCH) were included. This retrospective case study was conducted at PUMCH of the Chinese Academy of Medical Sciences and Peking Union Medical College(CAMS & PUMC) in China from January 1, 2003 to January 1, 2018. The continuous variable carbohydrate antigen 19-9(CA19-9) was converted into a categorical variable(cCA19-9) based on the normal reference range. Stages 0 to IIIA were merged into one category, while the remaining stages were grouped into another category. Pathological grade X(GX) was treated as a missing value. A multivariate Cox proportional hazards model was used to select variables to construct a nomogram. Discrimination and calibration of the nomogram were performed via the concordance index(C-index) and calibration plots. The performance of the nomogram was estimated using the calibration curve. Receiver operating characteristic(ROC) curve analysis and decision curve analysis(DCA) were performed to evaluate the predictive accuracy and net benefit of the nomogram, respectively.RESULTS Of these 142 GBC patients, 55(38.7%) were male, and the median and mean age were 64 and 63.9 years, respectively. Forty-eight(33.8%) patients in this cohort were censored in the survival analysis. The median survival time was 20 months. A series of methods, including the likelihood ratio test and Akaike information criterion(AIC) as well as stepwise, forward, and backward analyses, were used to select the model, and all yielded identical results. Jaundice [hazard ratio(HR) = 2.9; 95% confidence interval(CI): 1.60-5.27], cCA19-9(HR = 3.2; 95%CI: 1.91-5.39), stage(HR = 1.89; 95%CI: 1.16-3.09), and resection(R)(HR = 2.82; 95%CI: 1.54-5.16) were selected as significant predictors and combined into a survival time predictive nomogram(C-index = 0.803; 95%CI: 0.766-0.839). High prediction accuracy(adjusted C-index = 0.797) was further verified via bootstrap validation. The calibration plot demonstrated good performance of the nomogram. ROC curve analysis revealed a high sensitivity and specificity. A high net benefit was proven by DCA.CONCLUSION A nomogram has been constructed to predict the overall survival of GBC patients who underwent radical surgery from a clinical database of GBC at PUMCH. | Yi Bai Zhi-Song Liu Jian-Ping Xiong Wei-Yu Xu Jian-Zhen Lin Jun-Yu Long Fei Miao Han-Chun Huang Xue-Shuai Wan Hai-Tao Zhao | 2018 | World Journal of Gastroenterology2018，24，45： | 25 |
| 19 | Pilon fractures: a new classification and therapeutic strategies显示文摘 | TANG Xin TANG Pei-fu WANG Man-yi Lu De-cheng LIU Mo-zhen LIU Chang-jian LIU Yi SUN Li-zhong HUANG Liao-jiang YU Li ZHAO You-guang | 2012 | Chinese Medical Journal2012，，14： | 24 |
| 20 | Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma显示文摘Pancreatic ductal adenocarcinoma (PDAC) is the most common type of pancreatic cancer featured with high intra-tumoral heterogeneity and poor prognosis. To comprehensively delineate the PDAC intra-tumoral heterogeneity and the underlying mechanism for PDAC progression, we employed single-cell RNA-seq (scRNA-seq) to acquire the transcriptomic atlas of 57,530 individual pancreatic cells from primary PDAC tumors and control pancreases, and identified diverse malignant and stromal cell types, including two ductal subtypes with abnormal and malignant gene expression profiles respectively, in PDAC. We found that the heterogenous malignant subtype was composed of several subpopulations with differential proliferative and migratory potentials. Cell trajectory analysis revealed that components of multiple tumor-related pathways and transcription factors (TFs) were differentially expressed along PDAC progression. Furthermore, we found a subset of ductal cells with unique proliferative features were associated with an inactivation state in tumor-infiltrating T cells, providing novel markers for the prediction of antitumor immune response. Together, our findings provide a valuable resource for deciphering the intra-tumoral heterogeneity in PDAC and uncover a connection between tumor intrinsic transcriptional state and T cell activation, suggesting potential biomarkers for anticancer treatment such as targeted therapy and immunotherapy. | Junya Peng Bao-Fa Sun Chuan-Yuan Chen Jia-Yi Zhou Yu-Sheng Chen Hao Chen Lulu Liu Dan Huang Jialin Jiang Guan-Shen Cui Ying Yang Wenze Wang Dan Guo Menghua Dai Junchao Guo Taiping Zhang Quan Liao Yi Liu Yong-Liang Zhao Da-Li Han Yupei Zhao Yun-Gui Yang Wenming Wu | 2019 | Cell Research2019，29，9： | 24 |
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