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| 1 | The FTO/miR-181b-3p/ARL5B signaling pathway regulates cell migration and invasion in breast cancer显示文摘Background:N6-methyladenosine(m6A)RNA modification has been demonstrated to be a significant regulatory process in the progression of various tumors,including breast cancer.Fat mass and obesity-associated(FTO)enzyme,initially known as the obesity-related protein,is the first identified m6A demethylase.However,the relationship between FTO and breast cancer remains controversial.In this study,we aimed to elucidate the role and clinical significance of FTO in breast cancer and to explore the underlying mechanism.Methods:We first investigated the expression of FTO in breast cancer cell lines and tissues by quantitative reverse transcription-PCR(qRT-PCR),Western blotting,and immunohistochemistry.Wound healing assay and Transwell assay were performed to determine the migration and invasion abilities of SKBR3 and MDAMB453 cells with either knockdown or overexpression of FTO.RNA sequencing(RNA-seq)was conducted to decipher the downstream targets of FTO.qRT-PCR,luciferase reporter assay,and Western blotting were employed to confirm the existence of the FTO/miR-181b-3p/ARL5B axis.The biological function of ADP ribosylation factor like GTPase 5B(ARL5B)in breast cancer cells was evaluated by wound healing assay and Transwell invasion assay.Results:High FTO expression was observed in human epidermal growth factor receptor 2(HER2)-positive breast cancer,predicting advanced progression(tumor size[P<0.001],nuclear grade[P=0.001],peritumoral lymphovascular invasion[P<0.001),lymph node metastasis[P=0.002],and TNM stage[P=0.001])and poor prognosis.Moreover,FTO promoted cell invasion and migration in vitro.Mechanistically,RNA-seq and further confirmation studies suggested that FTO up-regulated ARL5B by inhibiting miR-181b-3p.We further verified that ARL5B also displayed carcinogenic activity in breast cancer cells.Conclusion:Our work demonstrated the carcinogenic activity of FTO in promoting the invasion and migration of breast cancer cells via the FTO/miR-181b-3p/ARL5B signaling pathway. | Yuanyuan Xu Shuang Ye Nan Zhang Shuhui Zheng Huatao Liu Kewen Zhou Ling Wang Yue Cao Peng Sun Tinghuai Wang | 2020 | Cancer Communications2020，40，10： | 24 |
| 2 | Identification of key genes affecting porcine fat deposition based on coexpression network analysis of weighted genes显示文摘Background:Fat deposition is an important economic consideration in pig production.The amount of fat deposition in pigs seriously affects production efficiency,quality,and reproductive performance,while also affecting consumers’choice of pork.Weighted gene co-expression network analysis(WGCNA)is effective in pig genetic studies.Therefore,this study aimed to identify modules that co-express genes associated with fat deposition in pigs(Songliao black and Landrace breeds)with extreme levels of backfat(high and low)and to identify the core genes in each of these modules.Results:We used RNA sequences generated in different pig tissues to construct a gene expression matrix consisting of 12,862 genes from 36 samples.Eleven co-expression modules were identified using WGCNA and the number of genes in these modules ranged from 39 to 3,363.Four co-expression modules were significantly correlated with backfat thickness.A total of 16 genes(RAD9A,IGF2R,SCAP,TCAP,SMYD1,PFKM,DGAT1,GPS2,IGF1,MAPK8,FABP,FABP5,LEPR,UCP3,APOF,and FASN)were associated with fat deposition.Conclusions:RAD9A,TCAP,SMYD1,PFKM,GPS2,and APOF were the key genes in the four modules based on the degree of gene connectivity.Combining these results with those from differential gene analysis,SMYD1 and PFKM were proposed as strong candidate genes for body size traits.This study explored the key genes that regulate porcine fat deposition and lays the foundation for further research into the molecular regulatory mechanisms underlying porcine fat deposition. | Kai Xing Huatao Liu Fengxia Zhang Yibing Liu Yong Shi Xiangdong Ding Chuduan Wang | 2022 | Journal of Animal Science and Biotechnology2022，13，1： | 3 |
| 3 | Effects of Angelica sinensis extracts on lipid oxidation in fish feeds and growth performance of juvenile Jian carp(Cyprinus carpio var. Jian)显示文摘The study was to explore the effect of the extracts of Angelica sinensis(EAs) on lipid oxidation in fish feeds compared with ethoxyquin(EQ) and the effect of dietary EAs on growth performance of carp(Cyprinus carpio var. Jian). Firstly, fish feeds were respectively added with EQ, and ethyl ether extract ethyl acetate extract(EAE), acetone extract, ethanol extract(EE) and aqueous extract(AQE) of Angelica sinensis, except for the control. The results showed that EAs and EQ inhibited lipid oxidation in fish feed(P < 0.05). Of all of the examined EAs, EAE showed the strongest protective effects against lipid oxidation(P < 0.05). Moreover, EAE at high concentrations showed a stronger effect on lipid oxidation compared with EQ(P < 0.05). Then, 7 experimental diets respectively supplemented with 0.0, 1.0, 2.0, 3.0, 4.0, 5.0 and 6.0 g/kg of EAE were fed to the respective treatment groups for 30 d. Four replicates were performed for each treatment group; 20 carp(mean weight: 12.10 ± 0.13 g) were in each replicate. The result indicated that dietary EAE improved the growth performance in carp(P < 0.05). The appropriate con centration of EAE for carp growth was estimated to be 3.643 g/kg diet. Thus, EAE could be used as a natural antioxidant in feeds for Jian carp. | Huatao Li Dandan Yang Zhihao Li Mingquan He Fengyi Li Jun Jiang Siyi Tang Peiyuan Peng Wenhao Du Yuting Ma Ying Liu | 2019 | Animal Nutrition2019，，1： | 2 |
| 4 | Rethinking phylogeographic structure and historical refugia in the rufous-capped babbler Cyanoderma ruficeps in light of range-wide genetic sampling and paleodistributional reconstructions显示文摘 | Peter A. HOSNER Huatao LIU A. Townsend PETERSON Robert G. MOYLE | 2015 | Current Zoology2015，61，5： | 2 |
| 5 | Solvents adjusted pure phase CoCO_(3) as anodes for high cycle stability显示文摘CoCO_(3) with high theoretical capacity has been considered as a candidate anode for the next generation of lithium-ion batteries(LIBs).However,the electrochemical performance of CoCO_(3) itself,especially the cyclic stability at high current density,hinders its application.Herein,pure phase CoCO_(3) particles with different particle and pore sizes were prepared by adjusting the solvents(diethylene glycol,ethylene glycol,and deionized water).Among them,CoCO_(3) synthesized with diethylene glycol(DG-CC)as the solvent shows the best electrochemical performance owing to the smaller particle size and abundant mesoporous structure to maintain robust structural stability.A high specific capacity of 690.7 mAh/g after 1000 cycles was achieved,and an excellent capacity retention was presented.The capacity was contributed by diverse electrochemical reactions and the impedance of DG-CC under different cycles was further compared.Those results provide an important reference for the structural design and stable cycle performance of pure CoCO_(3). | Liming LIU Xiaoxiao HUANG Zengyan WEI Xiaoming DUAN Bo ZHONG Long XIA Tao ZHANG Huatao WANG Dechang JIA Yu ZHOU Rui ZHANG | 2021 | Journal of Advanced Ceramics2021，10，3： | 0 |
| 6 | Identification and Expression Analysis of Abscisic Acid Signal Transduction Genes in Hemp Seeds显示文摘Abscisic acid(ABA)is involved in regulating diverse biological processes,but its signal transduction genes and roles in hemp seed germination are not well known.Here,the ABA signaling pathway members,PYL,PP2C and SnRK2 gene families,were identified from the hemp reference genome,including 7 CsPYL(pyrab-actin resistance1-like,ABA receptor),8 CsPP2CA(group A protein phosphatase 2c),and 7 CsSnRK2(sucrose nonfermenting1-related protein kinase 2).The content of ABA in hemp seeds in germination stage is lower than that in non-germination stage.Exogenous ABA(1 or 10μM)treatment had a significant regulatory effect on the selected PYL,PP2C,SnRK2 gene families.CsAHG3 and CsHAI1 were most significantly affected by exogenous ABA treatment.Yeast two-hybrid experiments were performed to reveal that CsPYL5,CsSnRK2.2,and CsSnRK2.3 could interact with CsPP2CA7 and demonstrate that this interaction was ABA-independent.Our results indicated that CsPYL5,CsSnRK2.2,CsSnRK2.3 and CsPP2CA7 might involve in the ABA signaling transduction pathway of hemp seeds during the hemp seed germination stages.This study suggested that novel genetic views can be brought into investigation of ABA signaling pathway in hemp seeds and lay the foundation for further exploration of the mechanism of hemp seed germination. | Cong Hou Kang Ning Xiuye Wei Yufei Cheng Huatao Yu Haibin Yu Xia Liu Linlin Dong | 2023 | Phyton-International Journal of Experimental Botany2023，92，7： | 0 |
| 7 | Influence of Transplantation in Different Stage on Growth and Yield of Spring Maize in Shanxi Early Mature Area显示文摘In order to explore a new mulch-free maize planting mode and the best seedling age for maize transplantation in Shanxi early mature area,a field experiment was carried out using completely randomized block design.Taking mulch-based direct sowing as the control group,effects of transplantation in different stage on maize growing process,plant height,three-ear leaf area in mature period,and yield and composition factors of maize were studied.Results indicate that growing process of maize seedlings transplanted in every stage is slightly slower than the control group.Transplantation has effect of reducing plant height and three-ear leaf area in mature period.There was no significant difference between the yield of maize transplanted on May 1st and the control group,and the yield declined only by 7% compared with mulch-based direct sowing(CK).Damages to maize seedlings by the local late frost must be noticed when maize is planted by the method of mulch-free transplantation in early mature area.Transplanting the seedlings earlier after the late frost is deemed as an appropriate period in this area. | Cong ZHAO Wei ZHANG Yanlong HAN Chunxia JIANG Huatao LIU Dongmei ZHANG Mingjing HUANG Xuefang HUANG Enke LIU | 2016 | Asian Agricultural Research2016，8，8： | 0 |
| 8 | Effect of Transplantation in Different Seedling Age on Growth and Yield of Spring Maize in Shanxi Early Mature Area显示文摘In order to explore a new mulch-free maize planting mode and the best seedling age for maize transplantation in Shanxi early mature area,a field experiment was carried out using completely randomized block design. Taking mulch based direct sowing as the control group,effects of transplantation in different seedling age on maize growing process,plant height,yield and composition factors of maize were analyzed.Results indicate that growing process of maize seedlings transplanted in different seedling age is slightly slower than the control group. Transplantation has the effect of reducing plant height. Although the yield of transplanted maize in different seedling age is lower than the control group. Besides,with the growth of seedling age of transplantation,the gap is widening. However,it is expected to realize mulch-free maize planting by transplantation in Shanxi early mature area. The yield of maize transplanted in two-leaf seedling age is not significantly different from the control group,and the yield is only 9% lower than the mulch-based direct sowing( CK). In conclusion,two-leaf period is the best seedling transplantation age for maize in Shanxi early mature area in this experiment condition. | Cong ZHAO Wei ZHANG Yanlong HAN Chunxia JIANG Huatao LIU Dongmei ZHANG Mingjing HUANG Xuefang HUANG Enke LIU | 2016 | Asian Agricultural Research2016，8，5： | 0 |
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