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23篇 您的检索式:作者名="Huaying An"
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1Inhibition of 12-lipoxygenase reduces proliferation and induces apoptosis of hepatocellular carcinoma cells in vitro and in vivo显示文摘BACKGROUND:12-lipoxygenase(12-LOX) has been reported to be an important gene in cancer cell proliferation and survival,and tumor metastasis.However,its role in hepatocellular carcinoma(HCC) cells remains unknown.METHODS:Expression of 12-LOX was assessed in a diethylnitrosamine-induced rat HCC model,and in SMMC-7721,HepG2 and L-02 cells using immunohistochemical staining and reverse transcriptase-polymerase chain reaction(RT-PCR).GST-π and Ki-67 were determined in vivo by immunohistochemical staining.Apoptosis was evaluated by TUNEL assay.Cell viability and apoptosis were determined by MTT assay and flow cytometry,respectively.Apoptosis-related proteins in SMMC-7721 and HepG2 cells were detected by Western blotting.RESULTS:Immunohistochemical staining and RT-PCR showed that 12-LOX was over-expressed in rat HCC and two HCC cell lines,while the expression was inhibited by baicalein,a specific inhibitor of 12-LOX.Baicalein inhibited cell proliferation and induced apoptosis in rat HCC and both cell lines in a dose-and time-dependent manner.Our in vivo study demonstrated that baicalein also reduced neoplastic nodules.Mechanistically,baicalein reduced Bcl-2 protein expression coupled with a slight increase of the expression of Bax and activation of caspase-3.Furthermore,baicalein inhibited the activation of ERK-1/2(phosphorylated).Interestingly,the effects of baicalein were reversed by 12(S)-HETE,a metabolite of 12-LOX.CONCLUSIONS:Inhibition of 12-LOX leads to reduced numbers of HCC cells,partially caused by increased apoptosis.12-LOX may be a potential molecular target for HCC prevention and treatment.Xi-Ming Xu,Guang-Jin Yuan,Jun-Jian Deng,Hong-Ting Guo,Miao Xiang,Fang Yang,Wei Ge and Shi-You Chen Cancer Center, Renmin Hospital of Wuhan University, Wuhan 430060, China Cancer Center, the 82nd Hospital of the Chinese PLA, Huai’an 223001, China Department of Physiology, Medical College of Wuhan University, Wuhan 430071, China Department of Physiology & Pharmacology, University of Georgia, Athens, GA 30602, USA 2012Hepatobiliary & Pancreatic Diseases International2012,11,2:20
2Ten‐Day Quadruple Therapy Comprising Proton‐Pump Inhibitor, Bismuth, Tetracycline, and Levofloxacin Achieves a High Eradication Rate for H elicobacter pylori Infection after Failure of Sequential Therapy显示文摘Ping‐I Hsu Wen‐Chi Chen Feng‐Woei Tsay Chih‐An Shih Sung‐Shuo Kao Huay‐Min Wang Hsien‐Chung Yu Kwok‐Hung Lai Hui‐Hwa Tseng Nan‐Jing Peng Angela Chen Chao‐Hung Kuo Deng‐Chyang Wu 2014Helicobacter2014,,1:3
3A Class of Second-Order Consensus Protocol in Multi-Agent Systems with Multiple Input Delays显示文摘This paper is concerned with the consensus problems for second-order multi-agent systems with multiple input delays. Different from all standard consensus algorithms with uniform delays, the authors aim to find the largest input-delay margin which can guarantee the consensus for the case when delays are nonuniform. Based on frequency domain analysis and matrix theory, an upper bound for maximum tolerable input-delay is given in terms of the relationship with scaling strengths and largest eigenvalue of the Lapalician matrix. Simulation results are provided to illustrate the obtained results.WU Huai GAO Kai AN Baoran 2019Journal of Systems Science & Complexity2019,32,5:2
4Research on Isolation and Clone of Embryonic Stem Cell-Like in Bovine显示文摘Bovine embryonic stem cell would be invaluable for researching the aspect of animal cloning, production transgenic animal and discussion of gene function in vitro. With the object of establishing an effective culture system for isolation and clone of bovine pluripotent stem cell, we cultured bovine embryos and mouse embryos including morula blastula and hatached blastula and obtained animal ICM on Primary marine embryonic fibroblast (Primary murine embryonic fibroblast, PMEF) feeder layer with tissue medium(DMEM supplemented with 15ml/100ml NBS ,0.1μmol/L Na2SeO3, 0. 1mmol/L β-mercaptoethanol, 1 000ng/ml LIF,10 ng/ml IGF, 1mmol/L necessary amino acid and 1mmol/L L-glutamine), then, we obtained mouse ICM and bovine ICM. Moreover, we isolated and cloned the 6 passage bovine ES like cells(12 cell lines) and 9 passage marine ES like cells (52 cell lines) deriving from bovine ICM and murine ICM respectively on the feeder layer of PMEF by disaggregating ICM and ES cell clones of bovine and murine into smaller clumps through digesting with 0. 125g/100ml trypsin and 0.02g/100ml EDTA and scattering with a glass needle. The pluripotency of both murine and bovine ES like cells was identified with morphological character, histochemistry identification, karyotype analysis and differentiation of ES cells in vitro or in vivo. This result showed that bovine embryonic stem cell and murine embryonic stem cell had developmental pluripotency.AN Li-long, YANG Qi, XIAO Mei, FENG Xiu-Liang, YANG Chun-rong, LEI An-min, GAO Zhi-min, DOU Zhong-ying and QIU Huai( Agriculture College of Zhanjiang Ocean University, Zhanjiang 524008 , P. R . China Key laboratory of AnimalReproductive Endocrinology and Embryo Biotechnology of Agricultural Ministry of China , Northwestern Scienceand Technology University of Agriculture and Forestry, yangling 712100 , P.R. China) 2002Agricultural Sciences in China2002,1,4:2
5MODELING OF SEDIMENT AND NONPOINT SOURCE POLLUTANT YIELD显示文摘For water and soil conservation and water pollution control, it is very important to simulate and predict the load of sediment and pollutant during storm-runoff. On the basis of analyzing the simultaneous measurements of flow, sediment and pollutants observed at watershed outlet, a practical sediment yield model is developed by standardizing the load rate. The results show that the standardized pollutant yield equals effective rainfall and the process of effective load yield is the same as effective rainfall hyetograph. Comparison with measured data show that this model is applicable to various pollutants.Huai’en LI, Xiaokang Hong and Bing SHEN Professor, Institute of Environmental Sciences, Xi’an University of Technology, Xi’an 710048, Shaanxi Province, P.R.China. Ph.D. studdent in Hydrology and Water Resources, Xi’an University of Techology, and Senior E 2001International Journal of Sediment Research2001,16,3:2
6Spatial-temporal Analysis of Emotions in Society in News显示文摘Spatial-temporal analysis of emotions in society has become popular in many studies integrating geography with the humanities,and has shown its influence on social sensing and geo-computation for social sciences.Emotions in society are often volatile,irrational,and vulnerable to the social environment.A critical challenge is to analyze changes in long-term and large-scale emotions in society.In this paper,we propose exploiting this challenge by using spatial-temporal analysis.After extracting emotional,temporal,and spatial information,a spatial standardization approach based on adataset of administrative district changes addresses the problem of Chinese toponym changes.Finally,over 1.7 million news data from the People’s Daily from 1956 to 2014 were collected to explore the changes,spatial distribution,and driving factors of emotions in society using spatial-temporal analysis.The experimental results found that the spatial-temporal analysis of emotions in society in the news is consistent with the results of related sociological research.An HUAI Xueying ZHANG Weicheng AI Tianyang CAO 2022Journal of Geodesy and Geoinformation Science2022,5,2:2
7Methane emissions from rice paddies natural wetlands, lakes in China: synthesis new estimate显示文摘Huai Chen Qiu’an Zhu Changhui Peng Ning Wu Yanfen Wang Xiuqin Fang Hong Jiang Wenhua Xiang Jie Chang Xiangwen Deng Guirui Yu 2012Glob Change Biol2012,,1:1
8Regioselective aminohalogenation of β-nitrostyrenes using NCS and NBS as nitrogen/halogen sources显示文摘Aminohalogenation reaction of β-nitrostyrenes with N-halosuccinimides(N-chloro and N-bromosuccinimides) has been successfully conducted by using nickel acetate as a catalyst in the presence of potassium carbonate and succinimide as co-additives.The reaction was easily performed at room temperature under nitrogen gas protection to give dihalorinaed haloamino products in good to excellent yields(60%-98%).The structure has been confirmed by X-ray crystal structure analysis.ZHI SanJun1,2,SUN Hao2,LIN Chen2,ZHANG GuangQian2,LI GuiGen4 & PAN Yi2,3 1 Department of Chemistry,Huaiyin Normal University,Huai’an 221003,China 2 School of Chemistry and Chemical Engineering,Nanjing University,Nanjing 210093,China 3 State Key Lab of Coordination Chemistry,Nanjing University,Nanjing 210093,China 4 Department of Chemistry and Biochemistry,Texas Tech University,Lubbock,Texas 79409-1061,USA 2010Science China Chemistry2010,53,1:1
9Characteristic Analysis of the Village and Town Structure——A Case of Jinhu County in Jiangsu Province,China显示文摘This thesis gives overview of the connotation of rural spatial structure,indicating 4 kinds of elements,namely nodus,passage,region and flow.Their spatial arrangement and interaction lead to the corresponding rural spatial structure.This thesis also offers panorama of the related theoretical progress of intra-county and rural areas,indicating that the research theories of regional spatial structure at present,mainly focus on macroscopic region or urban space,but there is shortage of researches on county spatial structure,especially microscopic rural spatial structure.On such basis,4 kinds of theoretical models and characteristics of regional spatial structure have been introduced.We build analysis index system of rural structural elements on the basis of 4 elements,and conduct rural spatial structural analysis,taking Jinhu County as an example.The result shows that rural spatial structure in Jinhu County is still at stage of nucleus-cluster development and rural system is not so sound.In addition,the passage network system of infrastructure has not taken shape,and county economy presents diseconomy of scale.The spatial interaction and association among towns in Jinhu County are weak.The characteristic analysis model of spatial structure in this research can objectively reflect regional characteristic of spatial structure.LUO Ya-li,ZHANG Chang-xin Huaiyin Institute of Technology,Huai’an 223001,China 2010Asian Agricultural Research2010,2,5:1
10Stomatin plays a suppressor role in non-small cell lung cancer metastasis显示文摘Objective:Metastasis is one of the key causes of high mortality in lung cancer.Aberrant DNA methylation is a common event in metastatic lung cancer.We aimed to identify new epigenetic regulation of metastasis-associated genes and characterize their effects on lung cancer progression.Methods:We screened genes associated with non-small cell lung cancer(NSCLC)metastasis by integrating datasets from the Gene Expression Omnibus(GEO)database.We obtained epigenetic-regulated candidate genes by analyzing the expression profile of demethylation genes.By overlapping analysis,epigenetically modulated metastasis-associated genes were obtained.Kaplan-Meier plotter(KM plotter)was utilized to assess the overall survival(OS)of stomatin in lung cancer.Immunohistochemistry(IHC)was conducted to determine the association between stomatin and metastasis-associated clinical indicators.Both in vitro and in vivo assays were performed to investigate the potential role of stomatin in metastasis.The regulation mechanisms of transforming growth factorβ1(TGFβ1)on stomatin were determined by Sequenom MassARRAY quantitative methylation and western blot assays.Results:A series of bioinformatic analyses revealed stomatin as the metastasis-associated gene regulated by DNA methylation.The KM plotter analysis showed a positive association between stomatin and the OS of lung cancer.IHC analysis indicated that the decreased stomatin expression is linked with advanced TNM stage.Loss-and gainof-function experiments displayed that stomatin could inhibit the migration and invasion of NSCLC cells.Furthermore,TGFβ1 repressed stomatin expression during epithelial-to-mesenchymal transition(EMT).The negative correlation between stomatin and TGFβ1 was also validated in advanced stage III lung tumor samples.The underlying mechanism by which TGFβ1 inhibits stomatin is due in part to DNA methylation.Conclusions:Our results suggest that stomatin may be a target for epigenetic regulation and can be used to prevent metastatic diseases.Huaying An Xiao Ma Mingyi Liu Xiaotong Wang Xundong Wei Wei Yuan Jie Ma 2019Chinese Journal of Cancer Research2019,31,6:1
11Field Emission of SiCN Thin Films Bombarded by Ar^+ Ions显示文摘SiCN thin films were synthesized by a radio frequency chemical vapor deposition (RFCVD) system on P\|type Si (1 0 0) wafers using C 2 H 4 , SiH 4 and N 2 as raw materials. In order to get rid of the oxygen absorbed on the surface and improve the characteristics of electron field emission, Ar + ions of low energy were used to bombard the samples. The field emission characteristics of SiCN thin films before and after Ar + bombardment were studied in the super vacuum environment of 10 -6 Pa. It was showed that the turn\|on field (defined as the point where the current\|voltage curve shows a sharp increase in the current density) decreased from 38 V/μm before bombardment to 25 V/μm after bombardment. And the maximum emission current density increased from 159.2 to 267.4 μA/cm 2 . The composition before and after Ar + bombardment was compared using X\|ray photoelectron spectroscopy (XPS). Our results illustrated that the field emission characteristics were improved after the bombardment of Ar + .Ma You\|peng, Li Jin\|chai  , Guo Huai\|xi, Lu Xian\|feng, Chen Ming\|an, Ye Ming\|sheng School of Physics and Technology, Wuhan University, Wuhan 430072, Hubei, China 2003Wuhan University Journal of Natural Sciences2003,8,03A:1
12Large area^(3)He tube array detector with modular design for multi‑physics instrument at CSNS显示文摘The multi-physics instrument(MPI)is the first user cooperative instrument at the China Spallation Neutron Source(CSNS).It was designed to explore the structures of complex materials at multiple scales based on the neutron total scattering technique.This imposes the requirements for the detector,including a high detection efficiency to reduce the measurement time and a large solid angle coverage to cover a wide range of momentum transfers.To satisfy these demands,a large-area array of 3He-filled linear position-sensitive detectors(LPSDs)was constructed,each with a diameter of 1 inch and pressure of 20 atm.It uses an orbicular layout of the detector and an eight-pack module design for the arrangement of 3He LPSDs,covering a range of scattering angles from 3°to 170°with a total detector area of approximately 7 m2.The detector works in air,which is separated from the vacuum environment to facilitate installation and maintenance.The characteristics of the MPI detector were investigated through Monte Carlo(MC)simulations using Geant4 and experimental measurements.The results suggest that the detectors are highly efficient in the wavelength range of the MPI,and an efficiency over 25%is achievable for above 0.1 A neutrons.A minimal position resolution of 6.4 mm full width at half maximum(FWHM)along the tube length was achieved at a working voltage of 2200 V,and a deviation below 2 mm between the real and measured positions was attained in the beam experiment.The detector module exhibited good consistency and an excellent counting rate capacity of up to 80 kHz,which satisfied the requirements of experiments with a high event rate.Observations of its operation over the past year have shown that the detector works steadily in sample experiments,which allows the MPI to serve the user program successfully.Lin Zhu Jian‑Rong Zhou Yuan‑Guang Xia Liang Xiao Hong Luo Xiao‑Juan Zhou Wen‑Qin Yang Bei‑Ju Guan Xing‑Fen Jiang Yan‑Feng Wang Hong Xu Hai‑Yun Teng Li‑Xin Zeng Jia‑Jie Li Lei Hu Ke Zhou Yong‑Xiang Qiu Pei‑Xun Shen Jun Xu Li‑Jiang Liao Xiao‑Zhuang Wang Gui‑An Yang Huai‑Chan Chen Ju‑Ping Xu Zhi‑Duo Li Song‑Lin Wang Jian Zhuang Yu‑Bin Zhao Jun‑Rong Zhang Wen Yin Zhi‑Jia Sun Yuan‑Bo Chen 2023Nuclear Science and Techniques2023,34,1:0
13Innovative Mechanism of Rural Organization Based on Self-Organization显示文摘The paper analyzes the basic situation of the formation of innovative rural organizations with the form of self-organization;reveals the features of self-organization,including the four aspects of openness of rural organization,innovation of rural organization far away from equilibrium,the non-linear response mechanism of rural organization innovation and the random rise and fall of rural organization innovation.The evolution mechanism of rural organization innovation is revealed according to the growth stage,the ideal stage,the decline and the fall stage.The paper probes into the basic restriction mechanism of the self-organization evaluation of rural organization from three aspects,including target recognition,path dependence and knowledge sharing.The basic measures on cultivating the innovative mechanism of rural organization are put forward.Firstly,constructing the dissipative structure of rural organization innovation;secondly,cultivating the dynamic study capability of rural organization innovation system;thirdly,selecting the step-by-step evolution strategy of rural organization innovation system.WANG Xing-jin,GAO Bing Jiangsu Vocational College of Finance & Economics,Huai’an 223003,China 2011Asian Agricultural Research2011,3,4:0
14Value and Path for Farmers' Citizenship Cultivation from Agri-ecological Security显示文摘Severe situation faced by China's agri-ecological security is elaborated.It is believed that cultivation of farmers' citizenship is the key to solving this problem,because it is favorable to consolidating farmers' independent and equal ideas,enhancing farmers' cooperative community idea,cultivating farmers' concept of rule by law,and strengthening farmers' fair and just moral concept.It is expected to make farmers fully exercise their civil rights and consciously perform their civil responsibilities.Finally,practical paths for cultivation of farmers' citizenship are put forward.These include improving market economy to promote equality of subjects,carrying out democratic practice to stimulate public participation,prospering cultural education to make clear public liability,propagating law in villages to carry forward spirit of rule by law,and making definite government responsibility to encourage social supervision.CHEN Ming School of Humanities,Huaiyin Institute of Technology,Huai’an 223003,China 2011Asian Agricultural Research2011,3,11:0
15Production Structure of Agriculture in Huai'an City显示文摘According to the data in 1990-2009 Huai'an Statistical Yearbook and Jiangsu Statistical Yearbook,production structure of agriculture in Huai'an City is analyzed.Result shows that economic efficiency of planting industry is significantly lower than that of other industry types of agriculture;and the production structure of agriculture in Huai'an City is unbalanced and needs further optimization.The reasons for the imbalance in industrial structure are various,such as the relatively low quality of agricultural employees,the backward marketing of planting,and the lack of standardization management.Finally,corresponding suggestions are put forward,including developing education,conducting vocational training,improving the human capital stock of Huai'an City,cultivating the leading enterprises for agricultural product processing,and realizing the scale agglomeration effect of agricultural production in planting industry.ZHU Hong-yun Huaiyin Institute of Technology,Huai’an 223001,China 2010Asian Agricultural Research2010,2,11:0
16机械力诱导MHC-Ⅰ构象变化增强TCR抗原识别及T细胞活化显示文摘CD8+T细胞主要通过T细胞表面受体(T cell recep tor,TCR)识别并与Ⅰ型主要组织性复合物提呈的抗原(pMHC-Ⅰ)相互作用[1]。TCR对刺激型抗原的识别在CD8+T细胞毒性和适应性免疫中发挥着关键作用。许多证据表明机械力可以延长TCR与刺激性pMHC作用的键合时间(bond lifetime)形成抗原特异性逆锁键(catch bond)[2],并且这种逆锁键对抗原识别非常重要。然而,机械力调控TCR抗原识别的具体结构机制仍不清楚。通过分子动力学模拟、单分子生物膜力学探针、磁镊、T细胞活化实验和动物模型对此问题展开了系统的研究[4]。发现作用在TCR-pMHC-Ⅰ复合体上的拉力可以作用在TCR-pMHC-Ⅰ复合体上的拉力可以打破MHC-I分子内部α1-α2和β2结构域间的相互作用,导致α1-α2结构域旋转并发生构象变化。力诱导的MHC-I构象变化可以进一步别构地调节TCR与刺激性抗原肽及α1-α2结构域的构象及相互作用,诱导产生新的氢键,增强TCR-pMHC-Ⅰ之间的键合时间,但并不能增强TCR与抑制性pMHC-Ⅰ之间的作用。当用点突变阻断这些新形成的氢键,或者α1-α2和β2结构域被二硫键锁住时,最佳力诱导的TCR-pMHC-Ⅰ作用的键合时间明显缩短并且T细胞的活化受到抑制。另外,在人TCR和HLA-A2相互作用中发现了类似机制,并且与肿瘤相关的HLA-A2点突变[3]可以通过限制HLA-A2α1--α2和β2结构域之间的构象打开减弱TCR对肿瘤抗原的识别及T细胞的功能。研究结果表明,机械力诱导的MHC-I构象变化对TCR抗原识别和T细胞活化非常重要,进一步地阐明了机械力调控TCR抗原识别机制,为临床肿瘤的免疫治疗和药物设计提供了新思路和新靶点。Peng Wu Tongtong Zhang Baoyu Liu Panyu Fei Lei Cui Rui Qin Huaying Zhu Danmei Yao Ryan Martinez Wei Hu Chenyi An Yong Zhang Junwei Liu Weiwei Yin Jie Sun Chun Zhou Xun Zeng Jianan Wang Brian Evavold Cheng Zhu Jizhong Lou Wei Chen 2019医用生物力学2019,34,A01:0
17The combination of benzamides/NCS as nitrogen/halogen sources for aminohalogenation of β-nitrostyrenes resulting in dichlorinated haloamides显示文摘The combination of benzamide and NCS was found to be an efficient nitrogen/halogen source for aminohalogenation of β-nitrostyrenes.The reaction was convenient to carry out by using 4-dimethylaminopyridine as the catalyst,resulting in vicinal dichlorinated haloamino nitroalkanes with opposite regiochemistry to that generated from other electron-deficient olefins observed previously.The reaction proceeded smoothly at room temperature with good yields and excellent regioselectivities.A mechanism involving a chloronium intermediate was proposed to explain the resulting regiochemistry.The current system explored a new type of nitrogen sources for aminohalogenation of functionalized olefins.ZHI SanJun1,2,MEI HaiBo1,ZHANG GuangQian1,SUN Hao1,HAN JianLin1,LI GuiGen4 & PAN Yi1,3 1 School of Chemistry and Chemical Engineering,Nanjing University,Nanjing 210093,China 2 School of Chemistry and Chemical Engineering,Huaiyin Normal University,Huai’an 221003,China 3 State Key Laboratory of Coordination Chemistry,Nanjing University,Nanjing 210093,China 4 Department of Chemistry and Biochemistry,Texas Tech University,Texas 79409-1061,USA 2010Science China Chemistry2010,53,9:0
18Oppugnation on the Efficiency of the Perfect Competitive Market显示文摘Huai'an Wei 2003Chinese Business Review2003,2,1:0
19MIDDLE EAR MECHANISMS IN PROTECTION AGAINST NOISE-INDUCED COCHLEAR DAMAG*显示文摘Quantitative estimation of sound transmission attenuation by different middle-ear protecting mecha-nisms was made with the use of a computer-based CM-cogram analyzing method (FFT of cochlear out-put in response to white noise)and other audiometric means.Strong reflex contraction of the middle-earmuscles in guinea pigs may cause a 5—20 dB transmission attenuation in different frequency ranges.Forimpulse noise exposure,the presence of an effective reflex at the lashing of every impulse may reduce thepost-exposure hearing threshold shift in 15 dB on an average.Increasing tension of the tympanic mem-brane by applying pressure to the ear-canal is also efficacious,especially for frequencies below 500 Hz whereattenuation of more than 10dB may result at a pressure difference of about 200mm H2O between the twosides of the membrane.Simulated opening of the eustachian tube helps only very insignificantly,withattenuation less than 5 dB.Destruction of the middle-ear structures(drum perforation。He, Zhizhou Lin, Huaying Lin, Xi Liang, Zhi'an 1989Chinese Journal of Acoustics1989,8,2:0
20Rose solutions with three petals for planar 4-body problems显示文摘For planar Newtonian 4-body problems with equal masses, we use variational methods to prove the existence of a non-collision periodic choreography solution such that all bodies move on a rose-type curve with three petals.DENG ChunHua 1,2 , ZHANG ShiQing 1, & ZHOU Qing 3 1 Yangtze Center of Mathematics and College of Mathematics, Sichuan University, Chengdu 610064, China 2 Faculty of Mathematics and Physics, Huaiyin Institute of Technology, Huai’an 223001, China 3 Department of Mathematics, East China Normal University, Shanghai 200062, China 2010Science China Mathematics2010,53,12:0
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