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| 1 | Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas显示文摘 | Xiaotong WANG Xiaorui SONG Tong WANG Qihui ZHU Guoying MIAO Yuanxin CHEN Xiaodong FANG Huayong QUE Li LI Guofan ZHANG | 2013 | Current Zoology2013，59，1： | 3 |
| 2 | Abalone mariculture in China显示文摘 | Zhang Guofan Que Huayong Liu Xiao | 2004 | J Shellfish Res2004，23，4： | 1 |
| 3 | The transcription of iodothyronine deiodinase genes is regulated by thyroid hormone receptor in the Pacific oyster Crassostrea gigas显示文摘Thyroid hormones (THs) are indispensable for each phyla in Chordata, while their functions in the non-chordate invertebrates are indistinct. Studies on the TH system in non-chordate invertebrates are important for understanding the evolution of TH system and may be applied in aquaculture or biofouling control at the same time. Iodothyronine deiodinases are keys to studying the TH system, as they are critical enzymes in maintaining TH homeostasis by catalyzing the initiation and termination of the effects of thyroid hormone in vertebrates. Here, we report the primary physiological effects of T4, the outer ring deiodinase activity, and a similar transcription regulation of two oyster deiodinases by TH receptor (CgTR) in an invertebrate, Pacific oyster Crassostrea gigas. L-thyroxine (T4) may have an important physiological function in the oyster, suggested by the growth retardation effect of excessive T4 in umbo larvae stage. The outer ring deiodinase activity transforming T4 to T3 (3, 3', 5-triiodothyronine) was then detected in the Pacific oyster in vivo, which may be conducted by two oyster deiodinases (CgDx and CgDy). Transcription regulation of CgTR onto these two deiodinase genes was also verified by electrophoretic mobility shift assay and dual luciferase reporter assay in mammalian cells. These results contribute to a better understanding of the evolution of the TH system. | HUANG Wen XU Fei LI Li QUE Huayong ZHANG Guofan | 2019 | Journal of Oceanology and Limnology2019，37，4： | 1 |
| 4 | Relationships of growth and mortality to enzymatic activity,and the relative mRNA expression of cultured scallops Patinopecten yessoensis in the Yellow Sea, China显示文摘The bottom aquaculture of yesso scallop (Patinopecten yessoensis) has developed rapidly in the north of Yellow Sea, China, but not without accompanying productivity problems. We, therefore, conducted field surveys to investigate factors related to growth and mortality rates of bottom-cultured scallops after release. Specifically, we focused on the effects of total antioxidant capacity (TAOC) and three key enzymes involved in antioxidant and metabolic function:glutamic pyruvic transaminase (GPT), lactic dehydrogenase (LDH), and superoxide dismutase (SOD). Across summer and winter, we also measured the relative mRNA expression of GPT and SOD to understand their seasonal variation in the scallops, along with how such variation correlated to growth and mortality. Results show that bottom cultured scallops experienced mass mortality during the first six months post-release. During winter (December), scallops grew more rapidly and suffered less mortality than in summer (August). The observed lower performance probably resulted from less advantageous environmental factors during the summer seasons, such as high temperature and low dissolved oxygen. These environmental stressors enhance protein consumption while decreasing energetic resources in scallops. Furthermore, scallops in summer exhibited high antioxidant levels that probably competed for energy with process integral to growth and survival. These negative factors combined to elevate mortality rates. In conclusion, we provided evidence suggesting correlations between metabolic/antioxidant activity and growth and mortality of bottom-cultured yesso scallops. These correlations implied us an accurate method to estimate the performance of bottom culture system. Suggestions about innovative aquaculture techniques were also discussed in the study. Our results might provide a possible guideline to the improvement of bottom culture techniques for this commercially valuable seafood species. | LIU Chao WU Fucun QUE Huayong ZHANG Guofan | 2019 | Journal of Oceanology and Limnology2019，37，4： | 1 |
| 5 | Chromosome segregation in fertilized eggs from triploid Pacific oysters, Crassostrea glgas (Thunberg), following inhibition of polar body Ⅰ显示文摘 | QUE Huayong GUO Ximing ZHANG Fusui | 1997 | Biol Bull1997，193，： | 1 |
| 6 | Chromosome segregation in fertilized eggs from triploid Pacific oysters, Crassostrea gigas (Thunberg), following inhibition of polar body Ⅰ显示文摘 | Que Huayong Guo Ximing Zhang Fusui | 1997 | Biol Bull1997，193，： | 1 |
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