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47篇 您的检索式:作者名="Huazhong Shi"
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1Plant abiotic stress response and nutrient use efficiency显示文摘Abiotic stresses and soil nutrient limitations are major environmental conditions that reduce plant growth,productivity and quality.Plants have evolved mechanisms to perceive these environmental challenges,transmit the stress signals within cells as well as between cells and tissues,and make appropriate adjustments in their growth and development in order to survive and reproduce.In recent years,significant progress has been made on many fronts of the stress signaling research,particularly in understanding the downstream signaling events that culminate at the activation of stress-and nutrient limitation-responsive genes,cellular ion homeostasis,and growth adjustment.However,the revelation of the early events of stress signaling,particularly the identification of primary stress sensors,still lags behind.In this review,we summarize recent work on the genetic and molecular mechanisms of plant abiotic stress and nutrient limitation sensing and signaling and discuss new directions for future studies.Zhizhong Gong Liming Xiong Huazhong Shi Shuhua Yang Luis R.Herrera-Estrella Guohua Xu Dai-Yin Chao Jingrui Li Peng-Yun Wang Feng Qin Jigang Li Yanglin Ding Yiting Shi Yu Wang Yongqing Yang Yan Guo Jian-Kang Zhu 2020Science China(Life Sciences)2020,63,5:94
2The Flowering Repressor SVP Confers Drought Resistance in Arabidopsis by Regulating Abscisic Acid Catabolism显示文摘陆上的植物必须应付干旱应力幸存。在干旱应力下面,植物由增加它的生合成并且减少积累植物激素 abscisic 酸(骆驼毛的织物) 它的分解代谢。然而,响应干旱控制骆驼毛的织物分解代谢的规章的小径仍然保持大部分不清楚。这里,我们报导 flowering 抑压者短植物的阶段(SVP ) 被干旱应力和伙伴与 ABA 分解代谢小径基因 CYP707A1, CYP707A3 和 AtBG1 的倡导者区域导致,引起 CYP707A1 和 CYP707A3 的减少的表示但是在 Arabidopsis 的 AtBG1 的提高的表示离开。在 AtBG1 的 CYP707A1 和 CYP707A3 或 overexpression 的 Loss-of-function 变化能由增加细胞的骆驼毛的织物层次救 svp 异种植物的干旱过分敏感的显型。一起,我们的结果建议 SVP 是骆驼毛的织物分解代谢的一个中央管理者 ? 并且包含 SVP, CYP707A1/3,和 AtBG1 的一条规章的小径在植物反应起一个关键作用浇赤字和植物干旱抵抗。Zhen Wang Fuxing Wang Yechun Hong Juanjuan Yao Zhizhong Ren Huazhong Shi Jian-Kang Zhu 2018Molecular Plant2018,11,9:12
3Optical properties of the micro/nano structures of Morpho butterfly wing scales显示文摘The micro/nano structures of the wing scales in Morpho butterfly are responsible for the structural coloration,with a major part ascribed to interference and diffraction of light.The optical properties of the butterfly wings were investigated by simulating a two-dimensional model using rigorous coupled-wave analysis technique.It is proved that they depend strongly on the structural parameters,incidence angle and refractive index.The peak value and the peak wavelength of the reflection efficiency increase as the vertical periodic thickness increases.The peak value decreases observably,while both of the bandwidth and the peak wavelength increase when the number of the vertical periods decreases.Increase of the horizontal periodic width causes a decrease of the peak value and an increase of the peak wavelength,although the variations are small.The peak value decreases distinctly and the peak wavelength increases as the ambient refractive index increases,which corresponds to the variation in ambient conditions.The research reveals the mechanisms of the brilliant structural color in Morpho butterfly,and is of great significance to the design,manufacture and applications of the bionic micro/nano structures for gas detection.LIAO GuangLan,ZUO HaiBo,CAO YanBo & SHI TieLin State Key Laboratory of Digital Manufacturing Equipment and Technology,Huazhong University of Science and Technology,Wuhan 430074,China 2010Science China(Technological Sciences)2010,53,1:10
4Wetting Rate and Clay Content Effects on Interrill Erosion in Ultisols of Southeastern China显示文摘An aggregate stability test and a simulated rainfall test were conducted on four representative Ultisols from southeastern China. The soils selected, with clay contents ranging between 117 and 580 g kg-1 , were derived from shale and Quaternary red clay. The stability of aggregates (2–5 mm in diameter) obtained from the soil samples were determined by the Le Bissonnais method. For determination of infiltration, runoff, and erosion, the soil samples were packed in 30 cm × 60 cm trays, wetted at rates of 2, 10, and 60 mm h-1 , and then exposed to simulated rainfall at 60 mm h-1 for 1 h. The results indicated that both aggregate stability and slaking caused by fast wetting increased with increasing clay content. The effect of wetting rate (WR) on infiltration and seal formation varied with clay contents. In the soil with low clay content (sandy loam), the infiltration rate was affected slightly by WR due to low aggregate stability and slaking. In the soils with medium clay content (silt clay loam and clay), WR affected infiltration significantly due to the high aggregate slaking force. In the soil with high clay content, the effect of WR on infiltration was significant, but not as evident as in the soils with medium clay content, which may be related to high aggregate stability by wetting partially compensating for slaking force. The effect of WR on soil loss was similar to that of runoff, but more pronounced. The findings from this study indicated that the relationship between wetting rate and clay content should be considered when predicting interrill erosion in Ultisols.YAN Feng-Ling, SHI Zhi-Hua , CAI Chong-Fa and LI Zhao-Xia Key Laboratory of Subtropical Agriculture & Environment, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070 (China) 2010Pedosphere2010,20,1:10
5Establishment and evaluation of the system of extracorporeal liver perfusion in pigs显示文摘BACKGROUND; In recent years, extracorporeal liver per- fusion (ECLP) has been regarded as a treatment of acute liver failure ( ALF ); but the system of ECLP has many problems. The purpose of this experiment was to detect the factors affecting the system of ECLP and to establish a sta- ble and effective system of ECLP. METHODS; Livers were harvested from health pigs, ac- cording to the different styles of perfusion and oxygena- tion, which were randomly divided into 3 groups. The liv- ers in group A (n =4) were subjected to single portal vein perfusion, oxygenating perfusion blood; the livers in group B (n =4) to dual ( portal vein and hepatic artery) vessel perfusion, oxygenating blood, together; and the livers in group C ( n = 4) to dual (portal vein and hepatic artery) vessel perfusion, but oxygenating blood, separately. The perfusion time, the data of bile production, and hemody- namic parameters of extracorporeal livers in each group were tested. The histological examination of liver tissues from each group was performed at the end of perfusion. RESULTS: The perfusion time of the liver in group A is significantly shorter than in groups B and C (P<0.05). At 1 , 3 , 6 hours after perfusion, the data of bile production and hemodynamic parameters of livers in group A were sta- tistically different from those of livers in groups B and C (P<0.05). At 1, 3, 6 hours after perfusion, the data of group B were not statistically different from those of livers in group C (P>0.05). But at 12 hours after perfusion, the data of group B were statistically different from those of liv- ers in group C (P<0.01). CONCLUSION: The system of ECLP, which is performed by dual ( portal vein and hepatic artery) vessel perfusionand oxygenating blood separately, is more stable and effec- tive to keep the function of extracorporeal liver.Jin Gong, Xi-Mo Wang, Gang Long, Zhong-Tao Guo, Tao Jiang and Shi Chen Wuhan, ChinaInstitute of Organ Transplantation, Tongji Hospital, Tongji Medical College , Huazhong University of Science and Technology, Wuhan 430030, China Department of Surgery, Tianjin People’s Hospital, Tianjin 300120, China 2005Hepatobiliary & Pancreatic Diseases International2005,4,1:7
6HOS3, an ELO-Like Gene, Inhibits Effects of ABA and Implicates a S-1-P/Ceramide Control System for Abiotic Stress Responses in Arabidopsis thaliana显示文摘Arabidopsis thaliana 的 hyper-osmotically 敏感的异种,指明的 hos3-1 (渗透地应答的基因的高表示) ,基于它 RD29A 的超被识别: 在有 NaCl 和骆驼毛的织物的处理之上的 LUC 倡导者熔化植物。这些回答含有破坏基因作为 RD29A 压力应答的小径的一个直接或间接的否定管理者。由定序 T-DNA 边阶的 flanking 区域,破坏基因在地点 At4g36830,这被决定,作为与高相同编码通常认为的蛋白质到 CIG30 (ELO2/FEN1 ) 注解了。CIG30 在合成被含有很长锁住丰满的酸(VLCFA ) ,它是为 sphingolipids 和 ceramides 的必要先锋。改变了骆驼毛的织物超敏性特征的压力回答,包括减少的根生长抑制并且与骆驼毛的织物处理减少了萌芽并且从叶子减少了水损失,被突变而产生之遗传的 hos3-1 和 hos3-2 异种展出。hos3-2 异种部分在它的抄本丰富被压制并且作为一个后退的特点被继承。进一步,在 35SCaMV 倡导者的控制下面的 HOS3 ORF 象 RD29A 一样恢复了野类型的 NaCl 根和骆驼毛的织物根生长敏感: 在变异的植物的 LUC 光。我们也这里证明 HOS3 野类型的基因机能上地补充 elo2 和 elo3 酵母异种的敏感到 monensin。而且, hos3-1 和 hos3-2 等位基因分享了增加的敏感到除草剂 Metolachlor,它在 VLCFA 的合成禁止酰链延伸,并且 HOS3 机能上地补充了 elo2 和 elo3 并且恢复了 VLCFA 的层次。一起,这些数据证实 HOS3 禁止调停骆驼毛的织物的压力回答并且含有控制为不能生活的应力发信号和回答的几个方面指的 VLCFA 小径和产品。结果也在有气孔的行为的控制为 ceramide 的一个角色提供支持。Tanya M. Quist Irina Sokolchik Huazhong Shi Robert J. Joly Ray A. Bressan Albino Maggio Meena Narsimhan Xia Li 2009Molecular Plant2009,2,1:7
7CLASSIFICATION OF GEAR FAULTS USING HIGHER-ORDER STATISTICS AND SUPPORT VECTOR MACHINES显示文摘Gears alternately mesh and detach in driving process, and then working conditions of gears are alternately changing, so they are easy to be spalled and worn. But because of the effect of additive gaussian measurement noises, the signal-to-noises ratio is low; their fault features are difficult to extract. This study aims to propose an approach of gear faults classification,using the cumulants and support vector machines. The cumulants can eliminate the additive gaussian noises, boost the signal-to-noises ratio. Generalisation of support vector machines as classifier, which is employed structural risk minimisation principle, is superior to that of conventional neural networks, which is employed traditional empirical risk minimisation principle. Support vector machines as the classifier, and the third and fourth order cumulants as input, gears faults are successfully recognized. The experimental results show that the method of fault classification combining cumulants with support vector machines is very effective.Lai Wuxing Zhang Guicai Shi Tielin Yang ShuziSchool of Mechanical Science and Engineering, Huazhong University of Science and Technology,Wuhan 430074, China 2002Chinese Journal of Mechanical Engineering2002,15,3:6
8Microscopic Calculation of Parameters of the sdg Interacting Boson Model for ^(104-110)Pd IsotopesLiu Yong~1 Shi Zhu-yi~2 Dan Han-jou~3 Sang Jian-ping~41 Institute of Particle Physics,Huazhong Normal University,Wuhan 4300702 Department of Physics,Guizhou Institute for Nationalities,Guiyang 5500253 Department of Physics,Xianning Teacher’s College,Xianning 4370004 Department of Physics,Wuhan University,Wuhan 430072 1995Chinese journal of nuclear physics1995,,3:5
9Protective effects of glycine pretreatment on brain-death donor liver显示文摘BACKGROUND: Morphological and functional changes commonly occur in livers of brain-death donors. Preven- tion of liver injury from brain-death will benefit the results of transplantation. This study was conducted to evaluate the protection effects of glycine on the liver of brain-death donor. METHODS: Fourty-two male Wistar rats were randomly divided into brain-death donor (BDD) group (B), glycine pretreatment group with BDD (G), and strychnine pre- treatment group with BDD(S). For these groups, brain death model was established in donor rats and liver trans- plantation was performed subsequently utilizing microsur- gical techniques. After the establishment of the model and during cold rinsing of liver donors or liver reperfusion of recipients, glycine was given at a dose of 0. 6 mmol, 25 μmol and 25 μmol in the group G, and a same dose of gly- cine and strychnine (1000 :1) was prescribed for the group S, but nothing for the group B. Before cold rinsing at 2 and 6 hours after portal vein(PV) reperfusion, blood sam- ples were taken from infrahepatic vena cava (IHVC) to de- termine the levels of alanine aminotransferase (ALT), as- partate aminotransferase (AST), tumor necrosis factor al- pha (TNF-α) and hyaluronic acid (HA). At 6 hours after PV reperfusion, graft samples were fixed for morphological observation and apoptosis of hepatocytes was detected using the TUNEL method. RESULTS: Before liver cold rinsing and at 2 and 6 hours af- ter PV reperfusion, the serum levels of ALT, AST, TNF-α, HA and apoptosis index (AI) in the groups B and S were significantly higher than those in the group G (P <0.05). There was no significant difference between the groups B and S (P>0.05). Electron microscopy showed that Kupffer cells were activated and hepatic cells injured more obvious- ly in the groups B and S than in the group G. CONCLUSION: Glycine pretreatment can improve the via-bility of the liver of the brain-death donor rat.Shui-Jun Zhang, Ji-Hua Shi, Zhe Tang, Yang Wu and Shi Chen Zhengzhou, China Department of Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China Institute of Organ Transplantation, Tongji Medical College , Huazhong University of Science and Technology, Wuhan 430030 , China 2005Hepatobiliary & Pancreatic Diseases International2005,4,1:5
10Expression of CD44,CD24 and ESA in pancreatic adenocarcinoma cell lines varies with local microenvironment显示文摘BACKGROUND:Emerging evidence suggests that pancreatic adenocarcinoma is hierarchically organized and sustained by pancreatic cancer stem cells.Furthermore,elimination of these cells is possible and therapeutically relevant.This study aimed to investigate the expression patterns of pancreatic cancer stem cell surface markers CD44,CD24 and ESA in pancreatic adenocarcinoma cell lines and explore the influence of their local microenvironment.METHODS:Flow cytometry was used to analyze the expression patterns of CD44,CD24 and ESA in five pancreatic adenocarcinoma cell lines (PANC-1,PC-2,MIA-Paca-2,AsPC-1 and BxPC-3).In addition,the capacity for sphereformation in serum-free medium of four cell lines (PANC-1,PC-2,MIA-Paca-2 and BxPC-3) was assessed.Then,the same assays were performed when tumor cell spheres were developed.The role of sonic hedgehog (SHH) in cell spheres from PANC-1 and MIA-Paca-2 were also assessed by RT-PCR.RESULTS:CD44 and CD24 were detected in PANC-1.Only CD44 expression was detected in PC-2,MIA-Paca-2 and AsPC-1.CD44,CD24 and ESA were all detected in BxPC-3.Tumor cell spheres developed in PANC-1 and MIA-Paca-2 in serumfree medium.This was accompanied by an increase in CD24 expression and a decrease in CD44 expression in PANC-1.Interestingly,the expression of CD44 and CD24 returned to initial levels once the medium was changed back from serumfree to serum-containing medium.No significant change in the expression of CD44 was detected in MIA-Paca-2.Furthermore,the relative quantification of SHH mRNA in PANC-1 cell spheres was significantly higher than that in cells cultured in the serum-containing medium.CONCLUSION:The expression patterns of the pancreatic cancer stem cell surface markers CD44,CD24 and ESA were diverse in different pancreatic adenocarcinoma cell lines and changed with their local microenvironment.Hong-Ji Wei,Tao Yin,Zhu Zhu,Peng-Fei Shi,Yuan Tian and Chun-You Wang Department of Pancreatic Surgery and Laboratory of General Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China 2011Hepatobiliary & Pancreatic Diseases International2011,10,4:5
11A NUMERICAL MODEL OF FAR-FIELD COD CONCENTRATION DISTRIBUTION FOR NATURAL RIVER显示文摘A far field numerical model is developed to simulate jet pollution into natural rivers. Its character is to compute the velocity and pollution concentration separately. The velocity is computed by SIMPLEC algorithm in boundary fitted coordinate systems. The pollution concentration distribution is computed by developed QUAL IIm and QAIIL 2D program. The present model was applied to the Xiantao section of the Hanjiang River a tributary of Yangtze River, and Xiangjiang River. The flow field and COD distribution are in good agreement with field data.Hou Guo xiang, Li Qi, Shi Zhong kun, Chen Cai kan Faculty of Traffic Sci. & Eng., Huazhong University of Science and Technology, Wuhan 430074, P.R.China Ye Ming, Deng Lian mu Yangtze Water Resources Protection Institute, Wuhan 430051, P.R.China 2000Journal of Hydrodynamics2000,12,1:3
12Spatial analysis increases efficiency of progeny testing of Chinese fir显示文摘We used spatial, global trend and post-blocking analysis to examine the effectiveness of a progeny trial in a tree breeding program for Chinese fir(Cunninghamia lanceolata(Lamb.) Hook) on a hilly site with an environmental gradient from hill top to bottom. Diameter at breast height(DBH) and tree height data had significant spatial auto-correlations among rows and columns. Adding a firstorder separable autoregressive term more effectively modelled the spatial variation than did the incomplete block(IB)model used for the experimental design. The spatial model also accounted for effects of experimental design factors and greatly reduced residual variances. The spatial analysis relative to the IB analysis improved estimation of genetic parameters with the residual variance reduced 13 and 19%for DBH and tree height, respectively; heritability increased35 and 51% for DBH and tree height, respectively; and genetic gain improved 3–5%. Fitting global trend and postblocking did not improve the analyses under IB model. The use of a spatial model or combined with a design model is recommended for forest genetic trials, particularly with global trend and local spatial variation of hilly sites.Liming Bian Renhua Zheng Shunde Su Huazhong Lin Hui Xiao Harry Xiaming Wu Jisen Shi 2017Journal of Forestry Research2017,28,3:3
13Measurement of a Three-dimensional Gas Temperature Field with Holographic Interferometry显示文摘MeasurementofaThree-dimensionalGasTemperatureFieldwithHolographicInterferometry¥CHENShaohua(YibinTeacher'sCollege,Yibin644007...CHEN Shaohua(Yibin Teacher’s College, Yibin 644007, CHN)ZHAO Xuxin HUANG Suyi SHI Dufang(Huazhong University of Science and Technology, Wuhan 430074, CHN) 1996Semiconductor Photonics and Technology1996,2,1:2
14Simultaneous pancreas-kidney transplantation with modified enteric drainage of exocrine pancreatic secretion显示文摘BACKGROUND: As a valid therapeutic option for patients with type 1 diabetes mellitus (IDDM) and secondary dia- betic nephropathy, simultaneous pancreas-kidney trans- plantation (SPK) remains more undeveloped than other solid organ transplantations due to the restrictions of surgi- cal techniques especially the modes of exocrine pancreatic secretion. The aim of this paper was to summarize our sin- gle-center experience in SPK with modified enteric drai- nage (ED). METHODS: From June 2000 to July 2003, 10 patients with IDDM associated with uremia received SPK. The pancrea- tic allograft exocrine secretion was drained into the proxi- mal jejunum via a side-to-side duodenojejunostomy with- out Roux-en-Y anastomosis. Quadruple immunosuppres- sive regimen consisted of induction of tacrolimus (TAC) / cyclosporine (CsA), mycophenolate mofetil (MMF), ste- roids and antibodies, which included antilymphocyte glo- bulin (ALG) or anti-CD25 monoclonal antibody. RESULTS: ED-SPK without Roux-en-Y anastomosis was successful in all 10 patients without serious complications such as pancreatitis, graft thrombosis and pancreatic fistu- la. The patients regained immediate kidney allograft func- tion and euglycemia with insulin-independence. Four pa- tients survived over one year. Episodes of acute rejection were observed in 4 patients, 3 of whom showed reversion after treatment of OKT3 or insulin. Early postoperative complications included peritoneal infection ( 2 patients ), wound infection (2) and renal hematoma (1). CONCLUSION: ED-SPK without Roux-en-Y anastomosis is safe and preferable to the patients with IDDM associated with uremia.Bin Liu, Chang-Sheng Ming, Fan-Jun Zeng, Bo Sha, Zhi-Shui Chen, Zhong-Hua Chen and Shi Chen Wuhan, China Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030 , China 2004Hepatobiliary & Pancreatic Diseases International2004,3,2:2
15Value of Commonality in Multiple Stage Assembly Products显示文摘This paper presents a systematic approach to estimate the value of commonality in products that involve multiple stage assemblies. In particular, the inventory savings are quantified with a mathematical model. The purpose is to assist product deisgn teams to make rational decisions in assigning unique parts or pursuing component standardization. In order to enhance the usability of the model, approximations are made to simplify the model and calculations.SHI Shuang-yuan, ZHANG Jin-long, CAI Shu-qin College of Management, Huazhong University of Science and TechnologyWuhan 430074, China 2002Systems Science and Systems Engineering2002,11,2:1
16The Arabidopsis thaliana salt tolerance gene SOS1 encodes a putative Na+/H+ antiporter显示文摘SHI Huazhong ISHITANI M KIM C S 0,,:1
17Overexpression of a plasma membrane Na+/H+ antiporter gene improves salt tolerance in Arabidopsis thaliana显示文摘SHI Huazhong LEE B H WU S J 0,,01:1
18Regulation of expression of the vacuolar Na+/H+ antiporter gene AtNHX1 by salt stress and abscisic acid显示文摘Huazhong Shi Jian-Kang Zhu 2002Plant Molecular Biology2002,,3:1
19The Arabidopsis spliceosomal protein SmEb modulates ABA responses by maintaining proper alternative splicing of HAB1显示文摘Abscisic acid(ABA)signaling is critical for seed germination and abiotic stress responses in terrestrial plants.PremRNA splicing is known to regulate ABA signaling.However,the involvement of canonical spliceosomal components in regulating ABA signaling is poorly understood.Here,we show that the spliceosome component Sm core protein SmEb plays an important role in ABA signaling.SmEb expression is up-regulated by ABA treatment,and analysis of Arabidopsis smeb mutant plants suggest that SmEb modulates the alternative splicing of the ABA signaling component HAB1 by enhancing the HAB1.1 splicing variant while repressing HAB1.2.Overexpression of HAB1.1 but not HAB1.2 rescues the ABA-hypersensitive phenotype of smeb mutants.Mutations in the transcription factor ABI3,4,or 5 also reduce the ABA hypersensitivity of smeb mutants during seed germination.Our results show that the spliceosomal component SmEb plays an important role in ABA regulation of seed germination and early seedling development.Yechun Hong Juanjuan Yao Huazhong Shi Yunjuan Chen Jian-Kang Zhu Zhen Wang 2021Stress Biology2021,1,1:1
20Overexpression of a plasma membrane Na^+/H^+ antiporter gene improves salt tolerance in Arabidopsis thaliana显示文摘Shi Huazhong Lee Byeong-ha 2002Nature biotechnology2002,,:1
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