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13篇 您的检索式：作者名="Hubener T"

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		题名	作者	年代	出处	被引量
	1	Tumor Antigen Specific Activation of Primary Human T-Cells Expressing a Virally Encoded Chimeric T-Cell Receptor Specific for p185HER2显示文摘We have developed and tested chimeric T-cell receptors (TCR) specific for p185HER2. In these experiments, retroviral vectors expressing the N29γ or N29ζ receptors were constructed in pRET6. Amphotropic viral producer cells were established in the GALV-based PG13 packaging cell line. Ficoll purified human peripheral blood lymphocytes (PBL) were virally transduced using an optimized protocol incorporating activation with immobilized anti-CD3/anti-CD28 monoclonal anti- bodies, followed by viral infection in the presence of fibronectin fragment CH296. Transduced cells were co-cultured with human tumor cell lines that overexpress (SK-OV-3) or underexpress (MCF7) p185HER2 to assay for antigen specific im- mune responses. Both CM+ and CD8+ T-cells transduced with the N29γ or N29ζ chTCR demonstrated HER2-specific anti- gen responses, as determined by release of Th1 like cytokines, and cellular cytotoxicity assays. Our results support the fea- sibility of adoptive immunotherapy with genetically modified T-cells expressing a chTCR specific for p185HER2.	杨建民 Michael S FRIEDMAN Christopher M REYNOLDS Marianne T HUBEN Lee WILKE Jennifer FULLER 李桥 Zelig ESHHAR James J MULE Kevin T MCDONAGH	2004	Journal of Microbiology and Immunology2004，2，4：	5
	2	In vivo anti-tumor activity of murine hematopoietic stem cells expressing a p185HER2-specific chimeric T-cell receptor gene显示文摘We have confirmed efficient anti-tumor activities of the peripheral lymphocytes transduced with a p185HER2-specific chimeric T-cell receptor gene both in murine and in human in our previous studies. To further test the feasibility of chimeric T-cell receptor in a bone marrow transplantation model, we first, made two murine tumor cell lines: MT901 and MCA-205, to express human p185HER2 by retroviral gene transduction. Murine bone marrow cells were retrovirally transduced to express the chimeric T-cell receptor and gene-modified bone marrow cells were transplanted into lethally irradiated mouse. Six months post transplantation, p185HER2-positive tumor cells:MT-901/HER2 or MCA-205/ HER2 was subcutaneously or intravenously injected to make mouse models simulating primary breast cancer or pulmonary metastasis. The in vivo anti-tumor effects were monitored by the size of the subcutaneous tumor or counting the tumor nodules in the lungs after India ink staining. The size of the subcutaneous tumor was significantly inhibited and the number of pulmonary nodules were significantly decreased in mouse recipients transplanted with chimeric T-cell receptor modified bone marrow cells compared with the control group. Our results suggest the efficient in vivo anti-tumor activities of chimeric T-cell receptor gene modified bone marrow cells.	JIAN MIN YANG MICHAEL S FRIEDMAN MARIANNE T HUBEN JENNIFER FULLER QIAO LI ALFRED E CHANG JAMES J MULE KEVIN T MCDONAGH	2006	Journal of Microbiology and Immunology2006，4，2：	3
	3	Implantation of colon cancers cells on intact and damaged colon mucosa and serosa: an experimental study in rat显示文摘	Huben J Lafullarde T Vermeulen P	1994	Acta Chir Belg1994，94，：	1
	4	The effect of oviposition time and genotype on egg quality characteristics in egg type hens显示文摘	T(u)mová E Zita L Huben(y) M et ol		0，，：	1
	5	Dissolved and particulate phosphorus forms in a eutrophic shallow lake 显示文摘	Selig U Hubener T Michalik M	2002	Aquat Sci2002，64，1：	1
	6	Implantation of colon cancers cells on intact and damaged colonmucosa and serosa:an experimental study in rat显示文摘	Huben J Lafullarde T Vermeulen P		0，，：	1
	7	Prognostic value of gastric intramucoral pH in surgical intensive care patients显示文摘	Gys T Hubens A Neels H	1988	Crit Care Med1988，16，12：	1
	8	Implantation of colon cancers cells on intact and damaged colon cancers ceils on intact and damaged colonmucosa and serosa:an experimental study in rat 显示文摘	Hubens J Lafullarde T Van Marck E	1994	Acta Chir Belg1994，94，5：	1
	9	Implantation of colon cancer cells on intact and damaged colon mucosa and serosa: an experimental study in the rat 显示文摘	Hubens G Lafullarde T Van Marck E	1994	Acta Chir Belg1994，94，5：	1
	10	Prognostic value of gastric intramural pH in surgical intensive care patients显示文摘	Gys T Hubens A Neels H	1988	Crit Care Med1988，16，12：	1
	11	Implantation of colon cancer cells on intact and damaged colon mucosa and serosa: an experimental study in the rat显示文摘	Hubens G Lafullarde T Van-Marck E	1994	Acta Chir Belg1994，94，5：	1
	12	Down-stream changes in phytoplankton composition and biomass in a lowland riv- er-lake system (Warnow River, Germany ) 显示文摘	Bahnwart M Hubener T Schubert H	1998	Hydrobiolo- gia1998，391，13：	1
	13	Implantation of colon cancer cells on intact and damaged colon mucosa and serosa:an experimental study in rat显示文摘	Huben J Lafullarde T Vermeulen P	1994	Acta Chir Belg1994，94，5：	1

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