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| 1 | Cell cycle and cell signal transduction in marine phytoplankton显示文摘作为单细胞的浮游植物,因此,一张海洋的浮游植物人口的生长直接源于一个细胞周期的结束房间环境通讯是包含信号 transduction 小径调整细胞周期前进并且贡献生长的一个重要方法,新陈代谢和主要生产并且在海洋的浮游植物对他们的包围环境作出回应。Cyclin-CDK 和 CaM/Ca 2+ 是控制房间周期和信号 transduction 小径的实质上关键的管理者,它穿上重要价值基本研究和应用生物工学。评论进步在这个研究领域里做了的这篇论文,包含房间,房间周期蛋白质由环境因素在特殊水平,毒素水藻的房间周期依赖者毒素生产和房间周期前进浮游植物被评为一个终端事件的一个标记估计生长。 | LIU Jingwen JIAO Nianzhi CAI Huinong | 2006 | Progress in Natural Science:Materials International2006，16，7： | 5 |
| 2 | Noninvasive method to drive medical micro-robots显示文摘A noninvasive method to drive medical micro-robots into endocoeles has been proposed and a kind of medical micro robot driven by the method has been designed. The robot has a spirally grooved impeller. When the spirally grooved impeller rotates, a hydrodynamic film between the robot and endocoeles is formed because of mucus existence in endocoeles. The generated axial thrust force of the impeller can drive robots to move. Because the hydrodynamic film is formed when the robot moves in endocoeles, injury may be prevented, so it can alleviate or avoid pain of sufferers. The generated axial thrust force of the impeller has been estimated according to the hydrodynamic lubrication theory and has been confirmed by experiments. | ZHOU Yinsheng HE Huinong GU Daqiang AN Qi QUAN Yongxin | 2000 | Chinese Science Bulletin2000，45，7： | 3 |
| 3 | The effect of soybean trypsin inhibitor on the degradation of myofibrillar proteins by an endogenous serine proteinase of crucian carp显示文摘 | JIANG Xinjing ZHANG Zhijun CAI Huinong | 2006 | Food Chemistry2006，94，4： | 1 |
| 4 | The effect of soybean trypsin inhibitor on the degradation of myofibrillar proteins by an endogenous serine proteinase of crucian carp显示文摘 | JIANG Xinjing ZHANG Zhijun CAI Huinong | 2006 | Food Chemistry2006，94，4： | 1 |
| 5 | Research on Decoupling Control in Temperature and Humidity Control Systems 显示文摘 | CAI Weiming ZHU Songming HE Huinong | 2012 | Computer and Computing Technologies in Agriculture V2012，368，： | 1 |
| 6 | Cloning and characterization of a thermostable carboxylesterase from inshore hot spring thermophile Geobacillus sp.ZH1显示文摘The gene(741 bp) encoding carboxylesterase from the thermophilic bacterium Geobacillus sp.ZHl was cloned and overexpressed in Escherichia coli.The purified recombinant protein presented a molecular mass of about 40 kDa by SDS-PAGE analysis.Enzyme assays using p-nitrophenyl esters with different acyl chain lengths as the substrates confirmed its esterase activity,yielding highest specific activity with p-nitrophenyl acetate.Among the p-nitrophenyl esters tested,the carboxylesterase presented preference for p-nitrophenyl caprylate,but hydrolyzed p-nitrophenyl butyrate more efficiently.When p-nitrophenyl butyrate was used as a substrate,the recombinant carboxylesterase exhibited highest activity at pH 8.0 and 60℃.Almost no decrease in esterase activity was observed at 60℃for 3 h,and over 40% of activity was still maintained after incubation at 90℃for 3 h.These results indicate that Geobacillus sp.ZH1 recombinant esterase was thermostable.The enzymatic activity was inhibited by the addition of phenylmethylsulfonyl fluoride,indicating that it contains serine residue,which plays a key role in the catalytic mechanism.Except SDS and xylene,this esterase showed stability toward other tested detergents and organic solvents.Cloning,expression,and biochemical characterization of Geobacillus sp.ZH1 carboxylesterase lay a good foundation for its structural characterization and industrial application. | ZHU Yanbing LIU Guangming LI Hebin LIU Jingwen BAI Xiaoming CAI Huinong | 2012 | Acta Oceanologica Sinica2012，31，6： | 1 |
| 7 | Acteoside from Cistanche salsa Inhibits Apoptosis by 1-Methyl-4-phenylpyridinium Ion in Cerebellar Granule Neurons显示文摘 | Xiaoping Pu Zhihong Song Yanyun Li Pengfei Tu Huinong Li | 2003 | Planta Med2003，，01： | 1 |
| 8 | Formation of edible soybean and soybean-complex protein films by a cross-linking treatment with a new Streptomyces transglutaminase显示文摘 | Guocheng SU Huinong Cai Changyi Zhou | 2007 | Food Technol Bioteehnol2007，45，4： | 1 |
| 9 | The effect of soybean trypsin inhibitor on the degradation of myofibriUar MPIs by an endogenous serine proteinase of crucian carp显示文摘 | JIANG Xinjing ZHANG Zhijun CAI Huinong | 2006 | Food Chemistry2006，94，4： | 1 |
| 10 | The effect of soybean trypsin inhibitor on the degradation of myofibrillar proteins by an endogenous serine proteinase of crucian carp显示文摘 | JIANG Xinjing ZHANG Zhijun CAI Huinong | 2006 | Food Chemistry2006，94，4： | 1 |
| 11 | Design of an experimental platform to investigate the effects of audible sounds on plant growth显示文摘An experimental platform was developed to investigate the effects of audible sound(20 Hz to 20 MHz)on plant growth promotion,which included a microcontroller-based embedded system for audible sound adjustment and analysis.The direct digital frequency synthesis(DDFS)method was used to generate various waveforms of sound in the platform.Soundproof glass and mufflers were used to reduce background noise.The developed system was tested on various plants,including hydroponic tomatoes,celery and mung bean.The testing results showed that the developed platform could produce pure tone and mixing audible sound with high stability and accuracy,make octave analysis of the sound under experimental environments,and the background noise in the testing chamber of the platform was lower than 55 dB(A)when the compression engine was working.The developed experimental platform has a great potential on facilitating scientific research on acoustic biology effects on plants and collecting real-time experimental data. | Cai Weiming Zhu Songming Ning Wang He Huinong Ying Beihua | 2015 | International Journal of Agricultural and Biological Engineering2015，8，5： | 0 |
| 12 | Purification and biochemical characterization of manganesecontaining superoxide dismutase from deep-sea thermophile Geobacillus sp. EPT3显示文摘Thermostable SOD is a promising enzyme in biotechnological applications. In the present study, thermophile Geobacillus sp. EPT3 was isolated from a deep-sea hydrothermal field in the East Pacific. A thermostable superoxide dismutase(SOD) from this strain was purified to homogeneity by steps of fractional ammonium sulfate precipitation, DEAE-Sepharose chromatography, and Phenyl-Sepharose chromatography. SOD was purified 13.4 fold to homogeneity with a specific activity of 3 354 U/mg and 11.1% recovery. SOD from Geobacillus sp. EPT3 was of the Mn-SOD type, judged by the insensitivity of the enzyme to both KCN and H2O2. SOD was determined to be a homodimer with monomeric molecular mass of 26.0 k Da. It had high thermostability at 50°C and 60°C. At tested conditions, SOD was relatively stable in the presence of some inhibitors and denaturants, such as β-mercaptoethanol(β-ME), dithiothreitol(DTT), phenylmethylsulfonyl fluoride(PMSF), urea, and guanidine hydrochloride. Geobacillus sp. EPT3 SOD showed striking stability across a wide p H range from 5.0 to 11.0. It could withstand denaturants of extremely acidic and alkaline conditions, which makes it useful in the industrial applications. | ZHU Yanbing LI Hebin NI Hui LIU Jingwen XIAO Anfeng CAI Huinong | 2014 | Acta Oceanologica Sinica2014，33，12： | 0 |
| 13 | EXPERIMENTAL STUDY ON LUBRICATION BEHAVIOR OF DOUBLE ENVELOPING HOUK GLASSWOKM GEARING显示文摘EXPERIMENTALSTUDYONLUBRICA-TIONBEHAVIOROFDOUBLEENVELO-PINGHOUKGLASSWOKMGEARINGEXPERIMENTALSTUDYONLUBRICA-TIONBEHAVIOROFDOUBLE... | He Huinong,Zhou Yinsheng,Quan Yongxin,( Zhejiang, University) Wei Yunlong, Cao Xingjin, (Chongqing, University) | 1996 | Chinese Journal of Mechanical Engineering1996，9，1： | 0 |
| 14 | Effects of UVB on the expression of proliferating cell nuclear antigen (PCNA) in dinoflagellate Prorocentrum donghaiense Lu显示文摘PCNA has been considered as a useful marker for the estimation of growth rates of marine phytoplankton at the species level. Since dinoflagellates are noted for having many prokaryotic features in that they are the only eukaryotes to have permanently condensed chromosomes as well as lacking histones and a nucleosome, the sensitivity to UVB radiation and the validity of PCNA as a maker of growth rate in dinoflagellate need to be evaluated.Prorocentrum donghaiense Lu was investigated to valuate the UVB sensitivity in relation to cellular and molecular aspects of PCNA as a growth indicator.The effects of UVB radiation on PCNA were studied using the methods of western blots technology and whole-cell immunoflurescence with one mono-antibody. UVB changed the cell morphology, halted the growth and increased the cell size, even caused cell death to a certain extent after treatment with UVB radiation in P. donghaiense. Compared with the control, treating the algal cultures in exponential phases with UVB radiation for 24 h caused chromatin release and increases in protein levels of PCNA. | LIU Jingwen JIAO Nianzhi CAI Huinong HONG Huasheng | 2007 | Acta Oceanologica Sinica2007，26，4： | 0 |
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