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| 1 | Comprehensive strategy of conduit guidance combined with VEGF producing Schwann cells accelerates peripheral nerve repair显示文摘Peripheral nerve regeneration requires stepwise and well-organized establishment of microenvironment.Since local delivery of VEGF-A in peripheral nerve repair is expected to promote angiogenesis in the microenvironment and Schwann cells(SCs)play critical role in nerve repair,combination of VEGF and Schwann cells may lead to efficient peripheral nerve regeneration.VEGF-A overexpressing Schwann cells were established and loaded into the inner wall of hydroxyethyl cellulose/soy protein isolate/polyaniline sponge(HSPS)conduits.When HSPS is mechanically distorted,it still has high durability of strain strength,thus,can accommodate unexpected strain of nerve tissues in motion.A 10 mm nerve defect rat model was used to test the repair performance of the HSPS-SC(VEGF)conduits,meanwhile the HSPS,HSPS-SC,HSPS-VEGF conduits and autografts were worked as controls.The immunofluorescent co-staining of GFP/VEGF-A,Ki67 and MBP showed that the VEGF-A overexpressing Schwann cells could promote the proliferation,migration and differentiation of Schwann cells as the VEGF-A was secreted from the VEGF-A overexpressing Schwann cells.The nerve repair performance of the multifunctional and flexible conduits was examined though rat behavioristics,electrophysiology,nerve innervation to gastrocnemius muscle(GM),toluidine blue(TB)staining,transmission electron microscopy(TEM)and NF200/S100 double staining in the regenerated nerve.The results displayed that the effects on the repair of peripheral nerves in HSPS-SC(VEGF)group was the best among the conduits groups and closed to autografts.HSPS-SC(VEGF)group exhibited notably increased CD31+endothelial cells and activation of VEGFR2/ERK signaling pathway in the regenerated nerve tissues,which probably contributed to the improved nerve regeneration.Altogether,the comprehensive strategy including VEGF overexpressing Schwann cells-mediated and HSPS conduit-guided peripheral nerve repair provides a new avenue for nerve tissue engineering. | Ping Wu Zan Tong Lihua Luo Yanan Zhao Feixiang Chen Yinping Li Céline Huselstein Qifa Ye Qingsong Ye Yun Chen | 2021 | Bioactive Materials2021，6，10： | 4 |
| 2 | IL-1 beta synthesis by chondrocyte analyzed by 3D microscopy and flow cytometry : effect of rhein 显示文摘 | De Isla N G Yang J W Huselstein C | 2006 | Biorheology2006，43，34： | 1 |
| 3 | Phenotypic analysis of cell surface markers and gene expression of human mesenchymal stem cells and chondrocytes during monolayer expansion显示文摘 | Cournil-Henrionnet C Huselstein C Wang Y | 2008 | Biorheology2008，45，34： | 1 |
| 4 | Phenotypic analysis of cell surface markers and gene expression of human mesenchymal stem cells and chondrocytes during monolayer expansion显示文摘 | Cournil-Henrionnet C C Huselstein | 2008 | Bio- rheology2008，45，34： | 1 |
| 5 | IL-1β synthesis by chondrocyte analyzed by 3D microscopy and flow cytometry:effect of rhein显示文摘 | N G de Isla J W Yang C Huselstein | 2006 | Biorheology2006，3，43： | 1 |
| 6 | Ageing and failure modes of IGBT modules in high temperature power cyeling显示文摘 | SMET V FOREST F HUSELSTEIN J J | 2011 | IEEE Transactions on Industrial Electronics2011，58，1： | 1 |
| 7 | Ageing and failure modes of IGBT modules in high temperature power cycling显示文摘 | Smet V Forest F Huselstein J J | 2011 | IEEE Transactions on Industrial Electronics2011，58，1： | 1 |
| 8 | IL-lbeta synthesis by chondrocyte analyzed by 3D microscopy and flow cytometry:effect of rhein显示文摘 | de Isla NG Yang JW Huselstein C | 2006 | Biorheology2006，43，34： | 1 |
| 9 | Design of a 28 V-to-300 V/12 kW multicell interleaved flyback converter using interceU transformers显示文摘 | FOREST F GELIS B HUSELSTEIN J J | 2010 | IEEE Transactions on Power Electronics2010，25，8： | 1 |
| 10 | Ageing and failure modes of IGBT modules in high temperature power cycling显示文摘 | SMET V FOREST F HUSELSTEIN J J | 2011 | IEEE Transactions on Industrial Electronics2011，58，10： | 1 |
| 11 | Mesenchyrnal stern cells for cartilage engineering显示文摘 | Huselstein C Li Y He X | 2012 | Pioned Mater FAng2012，22，13： | 1 |
| 12 | IL - lbeta synthesis by chon- drocyte analyzed by 3D microscopy and flow cytometry:effect of Rheim 显示文摘 | De lsla NG Yang JW Huselstein C | 2006 | Biorheology2006，43，34： | 1 |
| 13 | Ageing and failure modes of IGBT devices in high-temperature power cycling显示文摘 | SMET V FOREST F HUSELSTEIN J J | 2011 | Indust Electron IEEE Trans2011，58，10： | 1 |
| 14 | Evaluation of Vce monitoring as a real-time method to estimate aging of bond wire-IGBT modules stressed by power cycling 显示文摘 | Smet V Forest F Huselstein J | 2013 | IEEE Transactions on Industrial Electronics2013，60，7： | 1 |
| 15 | Ageing and failure modes of IGBT modules in high-temperature power cy- cling 显示文摘 | SMET V FOREST F HUSELSTEIN J J | 2011 | IEEE transactions on industrial electronics2011，58，10： | 1 |
| 16 | Mesenchymal stem cel s for cartilage engineering显示文摘 | Huselstein C Li Y He X | | 0，，1： | 1 |
| 17 | Evaluation of human MMSCs cell cycle, viability and differentiation in micromass cul- ture 显示文摘 | Yang JW de Isla N Huselstein C | 2006 | Biorheology2006，43，3： | 1 |
| 18 | Evaluation of human MSCs cell cycle,viability and differentiation in micromass culture显示文摘 | Yang JW de Isla N Huselstein C | | 0，，： | 1 |
| 19 | Mesenchymal stem cells for cartilage engineering显示文摘 | Huselstein C Li Y He X | 2012 | Bio Med Mater Eng2012，22，69： | 1 |
| 20 | IL-1 beta synthesis by chondrocyte analyzed by 3D microscopy and flow cytometry: effect of Rhein 显示文摘 | de Isla NG Yang JW Huselstein C | 2006 | Biorheology2006，43，34： | 1 |
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