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| 1 | Tenascin C upregulates interleukin-6 expression in human cardiac myofibroblasts via toll-like receptor 4显示文摘AIM:To investigate the effect of Tenascin C(TNC)on the expression of pro-inflammatory cytokines and matrixmetalloproteinases in human cardiac myofibroblasts(CMF).METHODS:CMF were isolated and cultured from patients undergoing coronary artery bypass grafting.Cultured cells were treated with either TNC(0.1μmol/L,24 h)or a recombinant protein corresponding to different domains of the TNC protein;fibrinogen-like globe(FBG)and fibronectin typeⅢ-like repeats(TNⅢ5-7)(both 1μmol/L,24 h).The expression of the proinflammatory cytokines;interleukin(IL)-6,IL-1β,TNFαand the matrix metalloproteinases;MMPs(MMP1,2,3,9,10,MT1-MMP)was assessed using real time RT-PCR and western blot analysis.RESULTS:TNC increased both IL-6 and MMP3(P<0.01)mR NA levels in cultured human CMF but had no significant effect on the other markers studied.The increase in IL-6 mR NA expression was mirrored by an increase in protein secretion as assessed by enzymelinked immunosorbant assay(P<0.01).Treating CMF with the recombinant protein FBG increased IL-6mR NA and protein(P<0.01)whereas the recombinant protein TNⅢ5-7 had no effect.Neither FBG nor TNⅢ5-7 had any significant effect on MMP3 expression.The expression of toll-like receptor 4(TLR4)in human CMF was confirmed by real time RT-PCR,western blot and immunohistochemistry.Pre-incubation of cells with TLR4neutralising antisera attenuated the effect of both TNC and FBG on IL-6 mR NA and protein expression.CONCLUSION:TNC up-regulates IL-6 expression in human CMF,an effect mediated through the FBG domain of TNC and via the TLR4 receptor. | Azhar Maqbool Emma J Spary Iain W Manfield Michaela Ruhmann Lorena Zuliani-Alvarez Filomena O Gamboa-Esteves Karen E Porter Mark J Drinkhill Kim S Midwood Neil A Turner | 2016 | World Journal of Cardiology2016，8，5： | 4 |
| 2 | Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis显示文摘 | NATALIA I ALEXEI S IAIN A | 2003 | Nature2003，423，： | 1 |
| 3 | The conserved KMN network constitutes the core micmtubule-binding site of the kinetochore显示文摘 | IAIN M C JOSHUA S ELIZABETH M | 2006 | Cell2006，127，： | 1 |
| 4 | The IgA system: a comparison of structure and function in different species显示文摘 | Veerle S Iain R P | 2006 | Vet Res2006，，37： | 1 |
| 5 | Assessment of eccentric exercise-induced muscle damage of the elbow flexors by tensiomyography 显示文摘 | Angus M Hunter Stuart D R Galloway Iain J S | 2012 | Journal of Electromyography and Kinesiology2012，22，3： | 1 |
| 6 | A new algorithm for rapid tracking of approximate maximum power point in photovoltaic systems显示文摘 | IAIN S AGARWAL V | 2004 | IEEE Power Electronics Letters2004，2，1： | 1 |
| 7 | Heavy metal distribution in sediment profiles of the Pearl River estuary, South China显示文摘 | Li X D Onyx W H Wai Y S Lia Bma3- J Colesb Michael H Ramseyb Iain Thornton | 2000 | Applied Geochemistry2000，15，5： | 1 |
| 8 | Liver regeneration显示文摘 | Leonidas G K Iain H M Seymour I S | 2003 | J Am Coll Surg2003，197，： | 1 |
| 9 | An FFT-based Approach for Fast Acquisition in Spread Spectrum Communication Systems显示文摘 | Sascha M Iain S Stephen M | 2000 | Wireless Personal Communications2000，13，2： | 1 |
| 10 | Fully Bayesian Reconstruction from Single-photon Emission Computed Tomography Data显示文摘 | | 1997 | J Amer Statistic Assoc1997，92，437： | 1 |
| 11 | Potent vasorelaxant activity of the TMEM16A inhibitor T16Ainh‐A01显示文摘 | Alison J Davis Jian Shi Harry AT Pritchard Preet S Chadha Normand Leblanc Georgios Vasilikostas Zhen Yao AS Verkman Anthony P Albert Iain A Greenwood | 2013 | Br J Pharmacol2013，，3： | 1 |
| 12 | Generation of high-affinity chicken single-chain Fv antibody fragments for measurement of the pseudonitzschia pungens toxin domoic 显示文摘 | William J J F Iain S Joanna P R | 2006 | Acid applied And Environmental Microbiology2006，，： | 1 |
| 13 | Charactefisation of a lipomannan lipoglycan from the mycolic acid containing actinomycete Dietzia mar/s显示文摘 | IAIN C S | 2000 | Antonic van Leeuwenhoek2000，78，： | 1 |
| 14 | An FFT-based approach for fast acquisition in spread spectrum communication systems显示文摘 | SASCHA M S IAIN S STEPHEN M | 2000 | Wireless Personal Communications2000，13，： | 1 |
| 15 | An SNF2 Protein associated with nuclear RNA silencing and the spread of a silencing signal between cells in Arabidopsis显示文摘 | Lisa M S Olga P Iain S | 2007 | Plant Cell2007，19，： | 1 |
| 16 | Reduced expression of p27 is a novel mechanism of docetaxel resistance in breast cancer cells显示文摘 | Iain B Kawan S Sarah LM | 2004 | Breast Cancer2004，6，： | 1 |
| 17 | Reduced expression of p27 is a novel mechanism of docetaxel resistance in breast cancer cells显示文摘 | Iain B Kawan S Sarahl M | | 0，，05： | 1 |
| 18 | Interfacial conditions between a pressfit acetabular cup and bone during daily activities: Implications for achieving bone in-growth 显示文摘 | Iain R S Peiderer M | 2000 | Journal of Biomechanics2000，33，： | 1 |
| 19 | Urban environmental geochemistry oftrace metals显示文摘 | Coby S C W Li X Iain T | 2006 | Environmental Pollution2006，142，1： | 1 |
| 20 | The template update problem显示文摘 | IAIN M ISHIKAWA T BAKER S | 2004 | IEEE Transactions on PAMI2004，26，6： | 1 |
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