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| 1 | Differential gene expression of chemokines in KRAS and BRAF mutated colorectal cell lines:Role of cytokines显示文摘AIM:To study KRAS/BRAF mutations in colorectalcancer(CRC)that influences the efficacy of treatment.To develop strategies for overcoming combination of treatment.METHODS:Five colonic cell-lines were investigated:DLD-1 with KRAS(G13D)mutation,HT 29 and Colo205 with BRAF(V600E)mutation as well as the wild type(Wt)cell-lines Caco2 and Colo-320.DLD-1(KRAS),HT-29(BRAF)and Caco2(Wt)cell lines were treated with cytokines(TNFα50 ng,IL-1β1 ng and IFNγ50ng)and harvested at different time points(1-24 h).KRAS inhibition was performed by the siRNA-approach.Two colorectal cancer cells DLD-1 and Caco2 were used for KRAS inhibition.About 70%confluency were confirmed before transfection with small interferring RNA(siRNA)oligonucleotides.All the synthetic siRNA sequences were designed in our laboratory.Total RNA and protein was isolated from the cells for RT-PCR and Western blotting.Densitometry of the Western blotting was analyzed with the Image J software(NIH).Results are shown as mean±SD.RESULTS:RT-PCR analysis in non-stimulated cells showed a low basal expression of TNFαand IL-1βin the DLD-1 KRAS-mutated cell-line,compared to Caco2wild type.No detection was found for IL-6 and IFNγin any of the studied cell lines.In contrast,pro-angiogenic chemokines(CXCL1,CXCL8)showed a high constitutive expression in the mutated cell-lines DLD-1(KRAS),HT-29 and Colo205(BRAF),compared to wild type(Caco2).The anti-angiogenic chemokine(CXCL10)showed a high basal expression in wild-type,compared to mutated cell-lines.KRAS down-regulation by siRNA showed a significant decrease in CXCL1 and CXCL10gene expression in the DLD-1(KRAS)cell-line in comparison to wild type(Caco2)at 72 h after KRAS silencing.In contrast,the specific KRAS inhibition resulted in an up-regulation of CXCL1 and CXCL10.The results of our study show a higher expression of pro-angiogenic chemokines at basal level in mutated cell-lines,which was further increased by cytokine treatment.CONCLUSION:To summarize,basal chemokine gene expression for pro-angiogenic chemokines was high in mutated as compared to wild type cell-lines.This reflects the likely existence of a different microenvironment in tumours consistent of wild type or mutated cells.This may help to rationalize the choice of molecular targets for suitable therapeutic investigation in clinical studies. | Sajjad Khan Silke Cameron Martina Blaschke Federico Moriconi Naila Naz Ahmad Amanzada Giuliano Ramadori Ihtzaz Ahmed Malik | 2014 | World Journal of Gastroenterology2014，20，11： | 6 |
| 2 | Single-Dose Gamma-Irradiation Induces Up-Regulation of Chemokine Gene Expression and Recruitment of Granulocytes into the Portal Area but Not into Other Regions of Rat Hepatic Tissue显示文摘 | Ihtzaz Ahmed Malik Federico Moriconi Nadeem Sheikh Naila Naz Sajjad Khan Jozsef Dudas Tümen Mansuroglu Clemens Friedrich Hess Margret Rave-Fr?nk Hans Christiansen Giuliano Ramadori | 2010 | The American Journal of Pathology2010，，4： | 2 |
| 3 | LIPOCALIN-2 Is a Major Acute-Phase Protein in a Rat and Mouse Model of Sterile Abscess显示文摘 | Sadaf Sultan Matteo Pascucci Shakil Ahmad Ihtzaz Ahmad Malik Alberto Bianchi Pierluigi Ramadori Ghayyor Ahmad Giuliano Ramadori | 2012 | Shock2012，，2： | 1 |
| 4 | Immunodetection of cyclooxygenase-2 (COX-2) is restricted to tissue macrophages in normal rat liver and to recruited mononuclear phagocytes in liver injury and cholangiocarcinoma显示文摘 | Marta Wójcik Pierluigi Ramadori Martina Blaschke Sadaf Sultan Sajjad Khan Ihtzaz A. Malik Naila Naz Gesa Martius Giuliano Ramadori Frank C. Schultze | 2012 | Histochemistry and Cell Biology2012，，2： | 1 |
| 5 | Ferritin L is the Sole Serum Ferritin Constituent and a Positive Hepatic Acute-Phase Protein显示文摘 | Naila Naz Federico Moriconi Shakil Ahmad Ahmad Amanzada Sajjad Khan Sabine Mihm Guiliano Ramadori Ihtzaz Ahmed Malik | 2013 | Shock2013，，6： | 1 |
| 6 | Reabsorption of iron into acutely damaged rat liver:A role for ferritins显示文摘AIM To studied iron metabolism in liver, spleen, and serum after acute liver-damage, in relation to surrogate markers for liver-damage and repair.METHODS Rats received intraperitoneal injection of the hepatotoxin thioacetamide(TAA), and were sacrificed regularly between 1 and 96 h thereafter. Serum levels of transaminases and iron were measured using conventional laboratory assays. Liver tissue was used for conventional histology, immunohistology, and iron staining. The expression of acute-phase cytokines, ferritin light chain(FTL), and ferritin heavy chain(FTH)was investigated in the liver by q RT-PCR. Western blotting was used to investigate FTL and FTH in liver tissue and serum. Liver and spleen tissue was also used to determine iron concentrations.RESULTS After a short initial decrease, iron serum concentrations increased in parallel with serum transaminase(aspartate aminotransferase and alanine aminotransferase) levels, which reached a maximum at 48 h, and decreased thereafter. Similarly, after 48 h a significant increase in FTL, and after 72 h in FTH was detected in serum. While earliest morphological signs of inflammation in liver were visible after 6 h, increased expression of the two acute-phase cytokines IFN-γ(1 h) and IL-1β(3 h) was detectable earlier, with maximum values after 12-24 h. Iron concentrations in liver tissue increased steadily between 1 h and 48 h, and remained high at 96 h. In contrast, spleen iron concentrations remained unchanged until 48 h, and increased mildly thereafter(96 h). Although tissue iron staining was negative, hepatic FTL and FTH protein levels were strongly elevated. Our results reveal effects on hepatic iron concentrations after direct liver injury by TAA. The increase of liver iron concentrations may be due to the uptake of a significant proportion of the metal by healthy hepatocytes, and only to a minor extent by macrophages, as spleen iron concentrations do not increase in parallel. The temporary increase of iron, FTH and transaminases in serum is obviously due to their release by damaged hepatocytes.CONCLUSION Increased liver iron levels may be the consequence of hepatocyte damage. Iron released into serum by damaged hepatocytes is obviously transported back and stored via ferritins. | Ihtzaz Ahmed Malik Jorg Wilting Giuliano Ramadori Naila Naz | 2017 | World Journal of Gastroenterology2017，23，41： | 1 |
| 7 | Development of Finite Element Solver for Welding Analysis显示文摘Welding is a process of joining the similar or different metals.Improper welding process leads to inaccuracies and misalignments of structural members,causing high cost and delays in work.Therefore,it is essential to predict the temperature field during welding process.Different techniques can be used to predict the temperature field,which may lead to structure distortion.The present study aims to develop a finite element solver for transient heat conduction analysis.The final solution is calculated from the assumed solution and compared with the numerical computations.The solver is then modified for use of moving heat source.The modification comprise,change in governing equations with the inclusion of phase change.The moving heat source continuously increases the temperature during motion.When the heat source completes a pass,model is allowed to cool down in order to study the temperature distribution during cooling. | Farzeen Shahid Dr Ihtzaz Qamar Dr Abid Ali Khan M.Saqib Hameed | 2018 | Smart Construction Research2018，2，1： | 0 |
| 8 | Development of Finite Element Solver for Welding Analysis显示文摘Welding is a process of joining the similar or different metals. Improper welding process leads to inaccuracies and misalignments of structural members, causing high cost and delays in work. Therefore, it is essential to predict the temperature field during welding process. Different techniques can be used to predict the temperature field, which may lead to structure distortion. The present study aims to develop a finite element solver for transient heat conduction analysis. The final solution is calculated from the assumed solution and compared with the numerical computations. The solver is then modified for use of moving heat source. The modification comprise, change in governing equations with the inclusion of phase change. The moving heat source continuously increases the temperature during motion. When the heat source completes a pass, model is allowed to cool down in order to study the temperature distribution during cooling. | Farzeen Shahid Dr Ihtzaz Qamar Dr Abid Ali Khan M.Saqib Hameed | 2018 | Smart Construction Research2018，2，2： | 0 |
| 9 | Testing Procedure for Laboratory Scale Semi Cryogenic Combustion Chamber of LPRE with Problems Faced and Lessons Learned显示文摘Design, development, and testing of LPRE(Liquid Propellant Rocket Engine) are difficult and expensive tasks. Prior to full-scale design, it is indispensable to optimize important parameters at sub-scale. Propellants flow rates are low for a sub-scale or laboratory scale combustion chambers. It is hard to satisfy chamber cooling and chill feed lines quickly with low flow rates of propellants. This paper proposes a detailed procedure for testing of a laboratory scale semi-cryogenic combustion chamber. Many tests were conducted with a small scale adjustable length combustion chamber. The injection head of the chamber was interchangeable. Liquid-liquid pressure swirl injector and like impinging injectors were used with two different injection heads. Liquid oxygen and kerosene were used as oxidizer and fuel, respectively. Oxidizer to fuel mixing ratio was 0.29-0.45 and the total propellant mass flow rate was 0.06-0.1 kg/s. Problems were faced during testing, including, explosion in the combustion chamber, fuel injector blockage, unstable combustion, incomplete chilling and blockage of cooling water channel, etc. A detailed procedure is designed on the basis of the lessons learned which was experimentally proved. | KHAN Taj Wali QAMAR Ihtzaz | 2022 | Journal of Thermal Science2022，31，6： | 0 |
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