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| 1 | A quantitative study on morphological responses of osteoblastic cells to fluid shear stress显示文摘液体砍应力(FSS ) 广泛地关于它对造骨细胞的影响被探索。在 vitro,研究证明了细胞骨架在对 FSS 的细胞的回答是很重要的。然而,词法变化,将象另外的细胞的回答一样反映细胞骨架变化,是份量上很少在过去的年里学习了。因此,在造骨细胞的导致 FSS 的词法变化在这研究被确定。实时快速的词法回答被为 1 h 与 1.2, 1.6,和 1.9 Pa 的大小暴露造骨细胞到 FSS 观察。后来,造骨细胞肌动朊细胞骨架用玫瑰精 phalloidin 被标记并且遵守了使用荧光显微镜学。结果证明 1.6 和 1.9 Pa FFS 沿着液体流动的方向导致了重要细胞的延伸和重取向。与 FSS 大小的改进一起,而且,细胞骨架显著地聚集了更多。而且,细胞外的弄空 Ca2+ 的液体流动也被用来与 1.6 和 1.9 Pa 的大小为 1 h 刺激造骨细胞。没有词法变化在移开细胞外的钙以后被观察。我们的学习建议从 1.2 ~ 1.9 Pa 的 FSS 的水平能够影响细胞的形态学,和细胞外的钙可能在造骨细胞对 FSS 刺激的反应起一个作用。 | Xiaoli Liu Xu Zhang Imshik Lee | 2010 | Acta Biochimica et Biophysica Sinica2010，42，3： | 14 |
| 2 | Computational analysis of triplex formation of oligonucleotides: protonated and 5-methylated py-pu-py motif显示文摘 | Imshik Lee Chunli Bai Chen Wang Xinwen Wang | 1997 | Science in China Series B: Chemistry1997，，2： | 1 |
| 3 | Regulation of actin cytoskeleton via photolithographic micropatterning显示文摘Actin cytoskeleton plays crucial roles in various cellular functions.Extracellular matrix(ECM)can modulate cell morphology by remodeling the internal cytoskeleton.To define how geometry of ECM regulates the organization of actin cytoskeleton,we plated individual NIH 3T3 cells on micropatterned substrates with distinct shapes and sizes.It was found that the stress fibers could form along the nonadhesive edges of T-shaped pattern,but were absent from the opening edge of V-shaped pattern,indicating that the organization of actin cytoskeleton was dependent on the mechanical environment.Furthermore,a secondary actin ring was observed on 50μm circular pattern while did not appear on 30μm and 40μm pattern,showing a size-dependent organization of actin cytoskeleton.Finally,osteoblasts,MDCK and A549 cells exhibited distinct organization of actin cytoskeleton on T-shaped pattern,suggesting a cell-type specificity in arrangement of actin cytoskeleton.Together,our findings brought novel insight into the organization of actin cytoskeleton on micropatterned environments. | Fulin Xing Haimei Zhang Mengyu Li Hao Dong Xuehe Ma Shiyu Deng Fen Hu Imshik Lee Leiting Pan Jingjun Xu | 2023 | Journal of Innovative Optical Health Sciences2023，16，2： | 1 |
| 4 | Single molecular mechanics of a cholesterol-beating pullulan nanogel at the hydrophobic interfaces 显示文摘 | ImShik L Akiyoshi K | 2004 | Biomaterials2004，25，15： | 1 |
| 5 | Computational analysis of triplex formation of oligonucleotides: protonated and 5-methylated py-pu-py motif显示文摘 | Imshik Lee Chunli Bai Chen Wang Xinwen Wang | 1997 | Science in China Series B: Chemistry1997，，2： | 1 |
| 6 | Single-cell manipulation by two-dimensional micropatterning显示文摘Cells are highly sensitive to their geometrical and mechanical microenvironment that directly regulate cell shape,cytoskeleton and organelle,as well as the nucleus morphology and genetic expression.The emerging two-dimensional micropatterning techniques offer powerful tools to construct controllable and well-organized microenvironment for single-cell level investigations with qualitative analysis,cellular standardization,and in vivo environment mimicking.Here,we provide an overview of the basic principle and characteristics of the two most widely-used micropatterning techniques,including photolithographic micropatterning and soft lithography micropatterning.Moreover,we summarize the application of micropatterning technique in controlling cytoskeleton,cell migration,nucleus and gene expression,as well as intercellular communication. | Xuehe Ma Haimei Zhang Shiyu Deng Qiushuo Sun Qingsong Hu Yuhang Pan Fen Hu Imshik Lee Fulin Xing Leiting Pan | 2024 | Journal of Innovative Optical Health Sciences2024，17，1： | 0 |
| 7 | Structure Studies on Host-Guest Recognition Sensory Systems显示文摘Rhodamine B-ethylenediamine-β-cyclodextrins (RHB - β -CDen) and rhodamine B-β-cyclodextrins (RhB-β-CD) form inclusion complexes with many guest molecules, which can be used as nucleic acid probe. In this paper we determined the most stable conformations of RhB-β-CDen and RhB-β-CD by molecular mechanics and dynamics simulation. The interaction between RhB-β-CDen and two guest molecules, 1-borneol and cyclohexanol, have been investigated both theoretically and experimentally. The results show that the interaction between borneol and RhB-β-CDen is stronger than that between cyclohexanol and RhB-β-CDen. | Lin Jing YANG Xi Zeng FENG Imshik LEE Chun Li BAI(Institute of Chemistry, the Chinese Academy of Science,Beijing, 100080) | 1997 | Chinese Chemical Letters1997，8，8： | 0 |
| 8 | Regulation of intracellular Ca^2+/CaMKII signaling by TRPV4 membrane translocation during osteoblastic differentiation显示文摘Bone constantly remodels between resorption by osteoclasts and formation by osteoblasts;therefore the functions of osteoblasts are pivotal for maintaining homeostasis of bone mass.Transient receptor potential vanilloid 4(TRPV4),a type of mechanosensitive channel,has been reported to be a key regulator in bone remodeling.Howevei;the relationship between TRPV4 and osteoblast function remains largely elusive.Only little is known about the spatial distribution change of TRPV4 during osteoblastic differentiation and related signal events.Based on three-dimensional super-resolution microscopy,our results clearly showed a different distribution of TRPV4 in undifferentiated and differentiated osteoblasts,which reflected the plasma membrane translocation of TRPV4 along with prolonged differentiation.GSK1016790A(GSK101),the most potent agonist of TRPV4,triggered rapid calcium entry and calmodulin-dependent protein kinase II(CaMKII)phosphorylation via TRPV4 acti・vation in a differentiation-dependent manner,indicating that the abundance of TRPV4 at the cell surface resulting from differentiation may be related to the modulation of Ca2+response and CaMKII activity.These data provide compelling evidences for the plasma membrane translocation of TRPV4 during osteoblastic differentiation as well as demonstrate the regulation of downstream Ca2+/CaMKII signaling. | Fen Hu Yali Zhao Zhenhai Hui Fulin Xing Jianyu Yang Imshik Lee Xinzheng Zhang Leiting Pan Jingjun Xu | 2019 | Biophysics Reports2019，5，5： | 0 |
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