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| 1 | Broccoli sprout extract induces detoxification-related gene expression and attenuates acute liver injury显示文摘AIM: To investigate the effects of broccoli sprout extract(BSEx) on liver gene expression and acute liver injury in the rat.METHODS: First, the effects of BSEx on liver gene expression were examined. Male rats were divided into two groups. The Control group was fed the AIN-76 diet, and the BSEx group was fed the AIN-76 diet containing BSEx. After a 10-d feeding period, rats were sacrificed and their livers were used for DNA microarray and realtime reverse transcription-polymerase chain reaction(RT-PCR) analyses. Next, the effects of BSEx on acute liver injury were examined. In experiments using acute liver injury models, 1000 mg/kg acetaminophen(APAP) or 350 mg/kg D-galactosamine(D-Gal N) was used to induce injury. These male rats were divided into four groups: Control, BSEx, Inducer(APAP or D-Gal N), and Inducer+BSEx. The feeding regimens were identical for the two analyses. Twenty-four hours following APAP administration via p.o. or D-Gal N administration via i.p., rats were sacrificed to determine serum aspartate transaminase(AST) and alanine transaminase(ALT) levels, hepatic glutathione(GSH) and thiobarbituric acid-reactive substances accumulation and glutathioneS-transferase(GST) activity. RESULTS: Microarray and real-time RT-PCR analyses revealed that BSEx upregulated the expression of genes related to detoxification and glutathione synthesis in normal rat liver. The levels of AST(70.91 ± 15.74 IU/m L vs 5614.41 ± 1997.83 IU/m L, P < 0.05) and ALT(11.78 ± 2.08 IU/m L vs 1297.71 ± 447.33 IU/m L, P < 0.05) were significantly suppressed in the APAP + BSEx group compared with the APAP group. The level of GSH(2.61 ± 0.75 nmol/g tissue vs 1.66 ± 0.59 nmol/g tissue, P < 0.05) and liver GST activity(93.19 ± 16.55 U/g tissue vs 51.90 ± 16.85 U/g tissue, P < 0.05) were significantly increased in the APAP + BSEx group compared with the APAP group. AST(4820.05 ± 3094.93 IU/m L vs 12465.63 ± 3223.97 IU/m L, P < 0.05) and ALT(1808.95 ± 1014.04 IU/m L vs 3936.46 ± 777.52 IU/m L, P < 0.05) levels were significantly suppressed in the D-Gal N + BSEx group compared with the D-Gal N group, but the levels of AST and ALT in the D-Gal N + BSEx group were higher than those in the APAP + BSEx group. The level of GST activity was significantly increased in the D-Gal N + BSEx group compared with the D-Gal N group(98.04 ± 15.75 U/g tissue vs 53.15 ± 8.14 U/g tissue, P < 0.05).CONCLUSION: We demonstrated that BSEx protected the liver from various types of xenobiotic substances through induction of detoxification enzymes and glutathione synthesis. | Kazutaka Yoshida Yusuke Ushida Tomoko Ishijima Hiroyuki Suganuma Takahiro Inakuma Nobuhiro Yajima Keiko Abe Yuji Nakai | 2015 | World Journal of Gastroenterology2015，21，35： | 3 |
| 2 | Circumferential strain behavior during creep tests of brittle rocks显示文摘 | Y Fujii T Kiyama Y Ishijima J Kodama | 1999 | International Journal of Rock Mechanics and Mining Sciences1999，，3： | 2 |
| 3 | Helicobacter pylori CagA Phosphorylation-Independent Function in Epithelial Proliferation and Inflammation显示文摘 | Masato Suzuki Hitomi Mimuro Kotaro Kiga Makoto Fukumatsu Nozomi Ishijima Hanako Morikawa Shigenori Nagai Shigeo Koyasu Robert H. Gilman Dangeruta Kersulyte Douglas E. Berg Chihiro Sasakawa | 2009 | Cell Host & Microbe2009，，1： | 2 |
| 4 | Postoperative seizures:Epidemiology, pathology and prophylaxis显示文摘 | Manaka S Ishijima B Mayanagi Y | 2003 | Neurology Medicine Chir2003，43，： | 1 |
| 5 | Enhancement of osteoclastic bone resorption and suppression of osteoblastic bone formation in response to reduced mechanical stress do not occur in the absence of osteopontin 显示文摘 | Ishijima M Rittling SR Yamashita T | 2001 | J Exp Med2001，193，39： | 1 |
| 6 | Mission function control for slew maneuver of a flexible structure显示文摘 | Fujii H Ishijima S | 1989 | Journal of Guidance Control and Dynamics1989，12，6： | 1 |
| 7 | Cell显示文摘 | Ishijima A | 1998 | 92:1611998，92，： | 1 |
| 8 | Back analysis of tunnel lining deformation:development and application of passive resistance method显示文摘 | Najm K Ishijima Y | 1993 | Rock Mechanics and Rock Engineering1993，26，2： | 1 |
| 9 | Osteoporosis and reduction of residual ridge in edentulous patients 显示文摘 | Hirai T Ishijima T Hashikawa Y | 1993 | J ProsthetDent1993，69，1： | 1 |
| 10 | Novel antiinflammatory com- pounds from Rubus sieboldi, triterpenoids, are inhibitors of mammalian DNA polymerases 显示文摘 | Murakami C Ishijima K Hirota M | 2002 | Biochim Biophys Acta2002，1596，2： | 1 |
| 11 | Processing of fine-grained W materials without detrimental phases and their mechanical properties at 200-432K显示文摘 | Ishijima Y Kannari S | 2008 | Mater Sci Eng A2008，473，： | 1 |
| 12 | The immunohistochemical expression of bcl-2 oncoprotein in colorectal adenocarcinoma 显示文摘 | Ishijima N Miki C Ishida T | 1999 | Surg Today1999，29，7： | 1 |
| 13 | The evolving role of biomarkers for osteoarthritis 显示文摘 | Ishijima M Kaneko H Kaneko K | 2014 | Ther Adv Mus- culoskelet Dis2014，6，4： | 1 |
| 14 | Functional anatomy of levator veli palatini muscle and tensor veli palatini muscle in association with eustachian tube cartilage显示文摘 | Ishijima K Sando I Balaban CD | 2002 | Ann Otol Rhinol Laryngol2002，111，6： | 1 |
| 15 | Autocrine loop between TGF-beta1 and IL-beta through Smad3- and ERK- depoldent pathways in rat pancreatic stellate cells显示文摘 | Aoki H Ohnishi H Hama K Ishijima T Satoh Y Hanatsuka K | 2006 | Am J Physiol Cell Physiol2006，290，4： | 1 |
| 16 | Intra-articular hy- aluronic acid injection versus oral non-steroidal anti-inflam- matory drug for the treatment of knee osteoarthritis : a multi- center, randomized, open-label, non-inferiority trial 显示文摘 | Ishijima M Nakamura T Shimizu K | 2014 | Ar- thritis Res Ther2014，16，1： | 1 |
| 17 | Postoperative seizures : epidemiology, pathology, and prophylaxis 显示文摘 | Manaka S Ishijima B Mayanagi Y | 2003 | Neurol Med Chir ( Tokyo )2003，43，12： | 1 |
| 18 | Osteopontin deficiency protects jionts against destruction in anti-type Ⅱ collagen antibody induced arthritis in mice显示文摘 | Yumoto K Ishijima M Rittling SR | 2002 | Proc Natl Acad Sci USA2002，99，7： | 1 |
| 19 | Water fraction of lumbar vertebral bone marrow estimated from chemical shift misregistration on MR imaging:Normal variations with age and sex显示文摘 | Ishizaka H Horikoshi H | 1996 | AJ R1996，167，2： | 1 |
| 20 | Postoper- ive sei-zures: epidemiology, pathology, and prophylaxis 显示文摘 | Manaka s Ishijima B Mayanagi Y | 2003 | Neu-rol Med Chir2003，43，12： | 1 |
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