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| 1 | Differential gene expression profiling in the developed ovaries between the parthenogenetic and bisexual female rice water weevils,Lissorhoptrus oryzophilus Kuschel(Coleoptera:Curculionidae)显示文摘The rice water weevil,Lissorhoptrus oryzophilus Kuschel(Coleoptera:Curculionidae),reproduces by sex in the Southeastern United States,but reproduces by parthenogenesis in California and other invaded regions in Asia and Europe.The objective of this study was to create a parthenogenetic gene expression profile of the rice water weevil in order to gain a better insight into the molecular mechanisms of parthenogenesis in the weevil.Suppression subtractive hybridization(SSH) technique was employed for profiling differential gene expression in the developed ovary between the parthenogenetic and bisexual female rice water weevils.A total of 70 contigs were obtained,and the BLASTX search identified putatively 28 genes with differential functions.According to the cytological process of parthenogenesis,the tubulin alpha-1 chain and signal transduction genes etc.were selected for real time quantitative RT-PCR analyses,and their possible functions related to the molecular mechanism of parthenogenesis were discussed.The tubulin alpha-1 chain and some signal transduction genes may be related to the molecular mechanisms of parthenogenesis of the rice water weevil. | YANG Pu ZHOU WenWu ZHANG Qin CHENG JiaAn ZHU ZengRongx WAY MO | 2009 | Chinese Science Bulletin2009,54,20: | 8 |
| 2 | β-Glucosidase treatment and infestation by the rice brown planthopper Nilaparvata lugens elicit similar signaling pathways in rice plants显示文摘β-Glucosidase has been reported to induce the production of herbivore-induced plant volatiles. How-ever, how it works remains unclear. Here, we investigated the levels of salicylic acid (SA), jasmonic acid (JA), ethylene, and H2O2, all of which are known signaling molecules that play important roles in in-duced plant defense in rice plants treated with β-glucosidase, and compared these to levels in plants infested by the rice brown planthopper Nilaparvata lugens (Stl). Results showed that wounding and treatment by β-glucosidase increased the levels of SA, ethylene, and H2O2, but not JA, in all plants compared to control plants. The signaling pathways activated by β-glucosidase treatment are similar to those activated by an infestation by N. lugens, although the magnitude and timing of the signals elicited by the two treatments are different. This may explain why both treatments have similar volatile profiles and are equally attractive to the parasitoid Anagrus nilaparvatae Pang et Wang. | WANG Xia ZHOU GuoXin XIANG CaiYu DU MengHao CHENG JiaAn LIU ShuSheng LOU YongGen | 2008 | Chinese Science Bulletin2008,53,1: | 5 |
| 3 | Salicylic acid and ethylene signaling pathways are involved in production of rice trypsin proteinase inhibitors induced by the leaf folder Cnaphalocrocis medinalis(Guenée)显示文摘The roles of signaling pathways in the production of trypsin proteinase inhibitors(TrypPIs)in rice infested by the leaf folder(LF) Cnaphalocrocis medinalis were studied.Infestation by LF increased TrypPI levels in the leaves of rice plants at the tillering,booting and flowering stages but decreased TrypPI levels at the ripening stage;TrypPI levels in rice stems did not increase at any developmental stage.Infestation by LF at the tillering stage systemically increased TrypPI levels in leaves but not in stems;it also enhanced salicylic acid(SA)levels in leaves and stems,and the ethylene level released from plants.However,LF infestation did not increase JA concentrations.Exogenous application of SA or ethylene enhanced TrypPI levels in the leaves and stems of plants at the tillering stage,whereas treatment with both SA and ethylene induced lower levels of TrypPIs than treatment with SA or ethylene alone,suggesting an antagonistic effect of SA and ethylene on TrypPIs induction.The results suggest that both SA and ethylene signaling pathways are involved in the production of TrypPIs in rice induced by LF;moreover,the antagonistic effect of SA and ethylene may explain the changes in TrypPI levels seen at different plant developmental stages and in different organs. | WANG Xia HU LiuCheng ZHOU GuoXin CHENG JiaAn LOU YongGen | 2011 | Chinese Science Bulletin2011,56,22: | 5 |
| 4 | In vitro generation of osteochondral differentiation of human marrow mesenchymal stem cells in novel collagen–hydroxyapatite layered scaffolds显示文摘 | Jiaan Zhou Caixia Xu Gang Wu Xiaodong Cao Liangming Zhang Zhichen Zhai Zhiwen Zheng Xiaofeng Chen Yingjun Wang | 2011 | Acta Biomaterialia2011,,11: | 1 |
| 5 | Controlling the strontium-doping in calcium phosphate microcapsules through yeastregulated biomimetic mineralization显示文摘Yeast cells have controllable biosorption on metallic ions during metabolism.However,few studies were dedicated to using yeast-regulated biomimetic mineralization process to control the strontium-doped positions in calcium phosphate microcapsules.In this study,the yeast cells were allowed to pre-adsorb strontium ions metabolically and then served as sacrificing template for the precipitation and calcination of mineral shell.The pre-adsorption enabled the microorganism to enrich of strontium ions into the inner part of the microcapsules,which ensured a slow-release profile of the trace element from the microcapsule.The co-culture with human marrow stromal cells showed that gene expressions of alkaline phosphatase and Collagen-I were promoted.The promotion of osteogenic differentiation was further confirmed in the 3D culture of cell-material complexes.The strategy using living microorganism as‘smart doping apparatus’to control incorporation of trace element into calcium phosphate paved a pathway to new functional materials for hard tissue regeneration. | Miaojun Huang Tianjie Li Ting Pan Naru Zhao Yongchang Yao Zhichen Zhai Jiaan Zhou Chang Du Yingjun Wang | 2016 | Regenerative Biomaterials2016,3,5: | 1 |
| 6 | Study of enhancement-mode GaN pFET with H plasma treated gate recess显示文摘This letter showcases the successful fabrication of an enhancement-mode(E-mode)buried p-channel GaN fieldeffect-transistor on a standard p-GaN/AlGaN/GaN-on-Si power HEMT substrate.The transistor exhibits a threshold voltage(VTH)of−3.8 V,a maximum ON-state current(ION)of 1.12 mA/mm,and an impressive ION/IOFF ratio of 10^(7).To achieve these remarkable results,an H plasma treatment was strategically applied to the gated p-GaN region,where a relatively thick GaN layer(i.e.,70 nm)was kept intact without aggressive gate recess.Through this treatment,the top portion of the GaN layer was converted to be hole-free,leaving only the bottom portion p-type and spatially separated from the etched GaN surface and gateoxide/GaN interface.This approach allows for E-mode operation while retaining high-quality p-channel characteristics. | Xiaotian Gao Guohao Yu Jiaan Zhou Zheming Wang Yu Li Jijun Zhang Xiaoyan Liang Zhongming Zeng Baoshun Zhang | 2023 | Journal of Semiconductors2023,44,11: | 0 |