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| 1 | The crustal structures of the central Longmenshan along and its margins as related to the seismotectonics of the 2008 Wenchuan Earthquake显示文摘In 2010,a 500-km-long wide-angle reflection/refraction seismic profile was completed,running northwest from the central Sichuan Basin.This profile orthogonally crosses the meizoseismal area of great Wenchuan earthquake of 12 May 2008,which occurred in the central part of the Longmenshan.The profile also passes through the northwestern Sichuan Plateau,along which a new deep seismic sounding observation system was set up that was much improved over previous datasets and enabled abundant observations to be recorded.Seismic wave phase records that reflect the structural characteristics of different tectonic blocks,especially the complicated phase features associated with the Wenchuan earthquake,were calculated and analyzed in detail.A 2D crustal P-wave velocity model for the orogenic belt in the central Longmenshan and its margins was determined,and crustal structure differences between the stable Sichuan Basin and the thickened northwestern Sichuan Plateau were characterized.Lithological variations within the upper and lower crust in the interior of the plateau,especially a great velocity decrease and plastic rheological properties associated with strong lithologic weakening in lower crust,were detected.From west to east in the lower crust beneath the orogenic belt lying between the Sichuan Basin and the northwestern Sichuan Plateau,a giant shovel-like upwelling is observed that dips gently in the lower part and at higher angles in the upper part;this is inferred to be related to the fault systems in the central Longmenshan.An upwelling in the upper-middle crust along the eastern margin of the orogenic belt is associated with steeply dipping thrusts that strongly uplift the upper crust and crystalline basement beneath a central fault system in the Longmenshan.The data,combined with an understanding of the regional tectonic stress field and previous geological results,enable a discussion of basin-and-range coupling,orogenic tectonics,the crustal fault system,and the seismogenic tectonic environment of the central Longmenshan along the eastern margin of the Qinghai-Tibet Plateau. | JIA ShiXu LIU BaoJin XU ZhaoFan LIU Zhi FENG ShaoYing ZHANG JianShi LIN JieYan TIAN XiaoFeng LIU QiaoXia GUO WenBin | 2014 | Science China Earth Sciences2014，57，4： | 26 |
| 2 | Frequencies of the expression of main protein antigens from Helicobacter pylori isolates and production of specific serum antibodies in infected patients显示文摘AIM: To investigate the frequencies of the expression of main protein antigens of Helicobacter pylori(H pylori)isolates, such as UreB, VacA, CagA1, HpaA, NapA, FlaA and FlaB and the production of specific antibodies in sera from H pylori-infected patients, and to understand the correlations among the different clinical types of chronic gastritis and peptic ulcer and the infection and virulence of H pylori.METHODS: H pylori strains in biopsy specimens from 157patients with chronic gastritis and peptic ulcer were isolated and serum samples from the patients were also collected.The target recombinant proteins rUreB, rVacA, rCagA1,rHpaA, rNapA, rFlaA and rFlaB expressed by the prokaryotic expression systems constructed in our previous studies were collected through Ni-NTA affinity chromatography.Rabbit antisera against rUreB, rVacA, rCagA1, rHpaA,rNapA, rFlaA and rFlaB were prepared by using routine subcutaneous immunization. By using ultrasonic lysates of the isolates as coated antigens, and the self-prepared rabbit antisera as the first antibodies and commercial HRP-labeling sheep anti-rabbit IgG as the second antibody,expression frequencies of the seven antigens in the isolates were detected by ELISA. Another ELISA was established to detect antibodies against the seven antigens in sera of the patients by using the corresponding recombinant proteins as coated antigens, and the sera as the first antibody and HRP-labeling sheep anti-human IgG as the second antibody respectively. Correlations among the different clinical types of chronic gastritis and peptic ulcer and the infection and virulence of H pylori were statistically analysed.RESULTS: In the 125 isolates of H pylori, the positive rates of UreB, VacA, CagA1, HpaA, NapA, FlaA and Flab were 100%, 65.6%, 92.8%, 100%, 93.6%, 100% and 99.2%respectively. In the 125 serum samples from the H pyloriinfected patients, the positive rates of antibodies against recombinant UreB, VacA, CagA1, HpaA, NapA, FlaA and Flab were 100%, 42.4%, 89.6%, 81.6%, 93.6%, 98.4%and 92.8% respectively. H pylori strains were isolated from 79.6% (125/157) of the biopsy specimens, but no close correlations among the H pylori infection frequencies and different types of chronic gastritis and peptic ulcer could be found (P>0.05, x2 = 0.01-0.87). The VacA positive rate (82.40%) in the strains isolated from the specimens of patients with peptic ulcer and the anti-VacA positive rate (54.3%) in the sera from the patients were significantly higher than those (51.5%, 32.3%) from the patients with chronic gastritis (P<0.01, x2 = 13.19; P<0.05, x2 = 6.13).When analysis was performed in the different types of chronic gastritis, the VacA in the strains isolated from the specimems of patients with active gastritis showed a higher expression frequency (90.0%) than those from superficial (47.9%) and atrophic gastritis (30.0%) (P<0.05, x2 = 5.93;P<0.01,x2 = 7.50). While analysis was carried out in the strains isolated from the specimens with superficial (93.8%) and active gastritis (100%), NapA showed a higher expression frequency compared to that from atrophic gastritis (60.0%) (P<0.01, x2 = 8.88; P<0.05,x2= 5.00).CONCLUSION: The types of chronic gastritis and peptic ulcer and their severity are not associated with H pylori infection frequency but closely related to the infection frequency of different virulent H pylori strains. The optimal antigens for developing vaccine and diagnostic kit are UreB,FlaA, HpaA, FlaB, NapA and CagA1, but not VacA. | JieYan Ya-FeiMao Zhe-XinShao | 2005 | World Journal of Gastroenterology2005，11，3： | 15 |
| 3 | Construction of prokaryotic expression system of ureB gene from a clinical Helicobacter pylori strain and identification of the recombinant protein immunity显示文摘AIM: To clone ureB gene from a clinical isolate of Helicobacter pyloriand construct a prokaryotic expression system of the gene and identify immunity of the expressed recombinant protein. METHODS: ureBgene from a clinical Hpyloristrain Y06 was amplified by the high fidelity polymerase chain reaction technique. The target DNA fragment amplified from ureB gene was sequenced after T-A cloning. Prokaryotic recombinant expression vector pET32a inserted with ureB gene (pET32a-ureB) was constructed. The expression of recombinant UreB protein (rUreB) in E. coli BL21DE3 induced by isopropylthio-β-D-galactoside (IPTG) at different concentrations was examined by SDS-PAGE. Western blot using commercial antibodies against whole cell of Hpylori and an immunodiffusion assay using a self-prepared rabbit anti-rUreB antibody were applied to determine immunity of the target recombinant protein. ELISA was used to detect the antibody against rUreB in sera of 125 Hpyloriinfected patients and to examine rUreB expression in 109 Hpylori isolates. RESULTS: In comparison with the reported corresponding sequences, the nucleotide sequence homology of the cloned ureB gene was from 96.88-97.82% while the homology of its putative amino acid sequence was as high as 99.65-99.82%. The rUreB output expressed by pET32a-ureB-BL21DE3 was approximate 30% of the total bacterial proteins, rUreB specifically combined with the commercial antibodies against whole cell of Hpylori and strongly induced rabbits to produce antibody with a 1:8 immunodiffusion titer after the animals were immunized with the recombinant protein. Serum samples from all Hpyloriinfected patients were positive for UreB antibody and UreB expression were detectable in all tested Hpyloriisolates. CONCLUSION: A prokaryotic expression system with high expression efficiency of Hpylori ureBgene was successfully established. The expressed rUreB showed qualified immunoreactivity and antigenicity. High frequencies of UreB expression in different Hpyloriisolates and specific antibody against UreBin sera of Hpyloriinfected patients indicatet hat UreB is an excellent antigen candidate for developing H pylori vaccine. | Ya-FeiMao JieYan | 2004 | World Journal of Gastroenterology2004，10，7： | 12 |
| 4 | Construction of expression systems for flaA and flaB genes of Helicobacter pylori and determination of immunoreactivity and antigenicity of recombinant proteins显示文摘AIM: To clone flagellin genes A (flaA) and B (flaB) from a clinical strain of Helicobacter pylori (H pylori) and to construct prokaryotic expression systems of the genes and identify immunity of the fusion proteins.METHODS: The flaA and flaBgenes from a clinical H pylori isolate Y06 were amplified by high fidelity PCR. The nucleotide sequences of target DNA amplification fragments from the two genes were sequenced after T-A cloning. The recombinant expression vector pET32a inserted with flaA and flaB genes was constructed, respectively. The expressions of FlaA and FlaB fusion proteins in E. Coli BL21DE3 induced by isopropylthio-β-D-galactoside (IPTG)at different concentrations were examined by SDS-PAGE.Western blot using commercial antibodies against whole cell of H pylori and immunodiffusion assay using self-prepared rabbit antiserum against FlaA (rFlaA) or FlaB (rFlaB)recombinant proteins were applied to the determination of the fusion proteins immunity. ELISA was used to detect the antibodies against rFlaA and rFlaB in sera of 125 H pylori infected patients and to examine rFlaA and rFlaB expression in 98 clinical isolates of H pylori, respectively.RESULTS: In comparison with the reported corresponding sequences, the nucleotide sequence homologies of the cloned flaA and flaB genes were from 96.28-97.13% and 96.31-97.73%, and their putative amino acid sequence homologies were 99.61-99.80% and 99.41-100% for the two genes, respectively. The output of rFlaA and rFlaB expressed by pET32a-flaA-BL21DE3 and pET32a-flaBBL21DE3 systems was as high as 40-50% of the total bacterial proteins. Both rFlaA and rFlaB were able to combine with the commercial antibodies against whole cell of H pylori and to induce rabbits to produce specific antibodies with the same 1:2 immunodiffusion titers after the animals were immunized with the two recombinant proteins. Ninety-eight and zero point 4 and 92.80% of the serum samples from 125 patients infected with H pylori were positive for rFlaA and rFlaB antibodies, respectively.One hundred percent and 98.98% of the 98 tested isolates of H pylori were detectable for rFlaA and rFlaB epitopes,respectively.CONCLUSION: Two prokaryotic expression systems with high efficiency of H pylori flaA and flaB genes were successfully established. The expressed rFlaA and rFlaB showed satisfactory immunoreactivity and antigenicity. High frequencies of FlaA and FlaB expression in different H pylori clinical strains and the general existence of specific antibodies against FlaA and FlaB in H pylori infected patients strongly indicate that FlaA and FlaB are excellent antigen candidates for developing H pylori vaccine. | JieYan Shao-HuiLiang Ya-FeiMao Li-WeiLi Shu-PingLi | 2003 | World Journal of Gastroenterology2003，9，10： | 10 |
| 5 | Construction of prokaryotic expression system of ItB-ureB fusion gene and identification of the recombinant protein immunity and adjuvanticity显示文摘AIM: To construct ItB-ureBfusion gene and its prokaryotic expression system and identify immunity and adjuvanticity of the expressed recombinant protein.METHODS: The ureB gene from a clinical Helicobacter pylori (H pylon) strain Y06 and the ItB gene from Escherichia coli (E. coli) strain 44851 were linked into ItB-ureB fusion gene by PCR. The fusion gene sequence was analyzed after T-A cloning. A prokaryotic recombinant expression vector pET32a inserted with ltB-ureB fusion gene (pET32a-ltB-ureB) was constructed. Expression of the recombinant LTB-UreB protein (rLTB-UreB) in E. coliBL21DE3 induced by isopropylthio-β-D-galactoside (IPTG) at different concentrations was detected by SDS-PAGE. Western blot assays were used to examine the immunoreaction of rLTB-UreB by a commercial antibody against whole cell of Hpylori and a self-prepared rabbit anti-rUreB serum, respectively,and determine the antigenicity of the recombinant protein on inducing specific antibody in rabbits. GM1-ELISA was used to demonstrate the adjuvanticity of rLTB-UreB.Immunoreaction of rLTB-UreB to the UreB antibody positive sera from 125 gastric patients was determined by using ELISA.RESULTS: In comparison with the corresponding sequences of original genes, the nucleotide sequence homologies of the cloned ltB-ureB fusion gene were 100%. IPTG with different dosages of 0.1-1.0 mmol/L could efficiently induce pET32a-ltB-ureB-E, coli BL21DE3 to express the rLTB-UreB.The output of the target recombinant protein expressed by pET32a-ureB-E.coli BL21DE3 was approximately 35% of the total bacterial proteins, rLTB-UreB mainly presented in the form of inclusion body. Western blotting results demonstrated that rLTB-UreB could combine with the commercial antibody against whole cell of H pylori and anti-rUreB serum as well as induce rabbit to produce specific antibody. The strong ability of rLTB-UreB bindingbovine GM1 indicated the existence of adjuvanticity of the recombinant protein. All the UreB antibody positive sera from the patients (125/125) were positive for rLTB-UreB.CONCLUSION: A recombinant prokaryotic expression system with high expression efficiency of the target fusion gene ltB-ureB was successfully established. The expressed rLTB-UreB showed qualified immunogenicity, antigenicity and adjuvanticity. All the results mentioned above laid a firm foundation for further development of Hpylorigenetically engineered vaccine. | JieYan YuanWang Shi-HeShao Ya-FeiMao Hua-WenLi Yi-HuiLuo | 2004 | World Journal of Gastroenterology2004，10，18： | 6 |
| 6 | Helicobacter pylori lipopolysaccharide:Biological activities in vitro and in vivo,pathological correlation to human chronic gastritis and peptic ulcer显示文摘AIM: To determine the biological activity of Helicobacter pylori(H pylori) lipopolysaccharide (H-LPS) and understand pathological correlation between H-LPS and human chronic gastritis and peptic ulcer.METHODS: H-LPS of a clinical Hpylori strain and LPS of Escherichia coli strain O55:B5(E-LPS) were extracted by phenol-water method. Biological activities of H-LPS and E-LPS were detected by limulus lysate assay, pyrogen assay,blood pressure test and PBMC induction test in rabbits, cytotoxicity test in NIH 3T3 fibroblast cells and lethality test in NIH mice. By using self-prepared rabbit anti-H-LPS serum as the first antibody and commercial HRP-labeled sheep anti-rabbit sera as the second antibody, H-LPS in biopsy specimens from 126 patients with chronic gastritis (68 cases) or gastric ulcer (58 cases) were examined by immunohistochemistry.RESULTS: Fibroblast cytotoxicity and mouse lethality of H-LPS were weaker than those of E-LPS. But the ability of coagulating limulus lysate of the two LPSs was similar (+/0.5 ng/mL).At 0.5 h after H-LPS injection, the blood pressures of the 3 rabbits rapidly declined. At 1.0 h after H-LPS injection, the blood pressures in 2 of the 3 rabbits fell to zero causing death of the 2 animals. For the other one rabbit in the same group, its blood pressure gradually elevated. At 0.5 h after E-LPS injection, the blood pressures of the three rabbits also quickly declined and then maintained at low level for approximately 1.0 h. At 0.5 hafter injection with H-LPS or E-LPS, PBMC numbers of the rabbits showed a remarkable increase. The total positivity rate of H-LPS from 126 biopsy specimens was 60.3%(76/126). H-LPS positivity rate in the biopsy specimens from chronic gastritis (50/68, 73.5%) was significantly higher than that from gastric ulcer (26/58, 44.8%) (χ^2=10.77,P<0.01). H-LPS positivity rates in biopsy specimens from chronic superficial gastritis (38/48, 79.2%) and chronicactive gastritis (9/10, 90.0%) were significantly higher than that of the patients with atrophic gastritis (3/10, 30.0%)(χ^2=7.50-9.66,P<0.01). CONCLUSION: The biological activities of H-LPS were weaker than those of E-LPS, the activities of H-LPS of lowering rabbit blood pressure and inducing rabbit PBMC were relatively stronger. H-LPS may play a critical role in inducing inflammatory reaction in human gastritis. | Yi-HuiLuo JieYan Ya-FeiMao | 2004 | World Journal of Gastroenterology2004，10，14： | 5 |
| 7 | Prevalence, risk factors, and survival associated with pulmonary hypertension and heart failure among patients with underlying coronary artery disease: a national prospective, multicenter registry study in China显示文摘Background: Coronary artery disease (CAD) is the commonest cause of heart failure (HF), whereas pulmonary hypertension (PH) has not been established or reported in this patient population. Therefore, we assessed the prevalence, risk factors, and survival in CAD-associated HF (CAD-HF) complicated with PH.Methods: Symptomatic CAD-HF patients were continuously enrolled in this prospective, multicenter registry study. Echocardiography, coronary arteriography, left and right heart catheterization (RHC), and other baseline clinical data were recorded. Patients were followed up and their survival was recorded.Results: One hundred and eighty-two CAD-HF patients were enrolled, including 142 with HF with a preserved ejection fraction (heart failure with preserved ejection fraction [HFpEF];left ventricular ejection fraction [LVEF] ≥50%) and 40 with a reduced ejection fraction (heart failure with reduced ejection fraction [HFrEF];LVEF < 50%). PH was diagnosed with RHC in 77.5% of patients. Patients with PH showed worse hemodynamic parameters and higher mortality. HFrEF-PH patients had worse survival than HFpEF-PH patients. CAD-HF patients with an enlarged left ventricular end-diastolic diameter and reduced hemoglobin were at higher risk of PH. Nitrate treatment reduced the risk of PH. Elevated creatinine and mean pulmonary arterial pressure (mPAP), diastolic pressure gradient (DPG) ≥7 mmHg, and previous myocardial infarction (MI) entailed a higher risk of mortality in CAD-HF patients with PH.Conclusions: PH is common in CAD-HF and worsens the hemodynamics and survival in these patients. Left ventricle enlargement and anemia increase the risk of PH in CAD-HF. Patients may benefit from nitrate medications. Renal impairment, elevated mPAP, DPG ≥7 mmHg, and previous MI are strong predictors of mortality in CAD-HF-PH patients.Trial Registration: ClinicalTrials.gov, NCT02164526. | Li Huang Lingpin Pang Qing Gu Tao Yang Wen Li Ruilin Quan Weiqing Su Weifeng Wu Fangming Tang Xiulong Zhu Jieyan Shen Jingzhi Sun Guangliang Shan Changming Xiong Shian Huang Jianguo He | 2022 | Chinese Medical Journal2022，，15： | 4 |
| 8 | Construction of prokaryotic expression system of 2 148-bp fragment from cagA gene and detection of cagA gene,CagA protein in Helicobacter pylori isolates and its antibody in sera of patients显示文摘To construct a prokaryotic expression system of a Helicobacter pylori ( H pylori) cagA gene fragment and establish enzyme-linked immunosorbent assays-(ELISA) for detectin.g Ca.gA.and its antibody, so as to understand the manner in which the infection of CagA-expressing Hpylori(CagA+ Hpylori) isolates cause diseases. | JieYan YuanWang Shi-HeShao Ya-FeiMao Hua-WenLi Yi-HuiLuo | 2004 | World Journal of Gastroenterology2004，10，8： | 3 |
| 9 | Establishment of Helicobacter pylori infection model in Mongolian gerbils显示文摘AIM:To establish a stable and reliable model of Helicobacter pyloriinfection model in Mongolian gerbils and to observe pathological changes in gastric mucosa in infected animals.METHODS:Mongolian gerbils were randomly divided into 18 groups;6 groups were infected with Hpylori clinical strain Y06 (n=6, groups Y), 6 groups were infected with Hpylori strain NCTC11637 (n=6, roups N),and 6 uninfected groups as negative controls (n=4,groups C).Hpylori suspensions at the concentrations of 2×10^8 and 2×10^9CFU/mL of strain NCTC11637 and strain Y06 were prepared. The animals in three groups N and in three groups Y were orally challenged once with 0.5 mL of the low concentration of the bacterial suspension.The animals in another three groups N and in another three groups Y were orally challenged with 0.5mL of the high concentration of the bacterial suspension for 3 times at the intervals of 24 h,respectively.For the negative controls,the animals in six groups C were orally given with the same volume of Brucella broth at the corresponding inoculating time.The animals were killed after 2nd, 4^th and 6^th week after the last challenge and the gastric mucosal specimens were taken for urease test,bacterial isolation, pathological and immunohistochemical examinations.RESULTS:Positive isolation rates of Hpyloriin the animals of groups Y at the 2nd, 4^th and 6^th week after one challenge were 0%, 16.7% and 66.7%, while in the animals of groups N were 0%, 0% and 16.7%, respectively.Positive isolation rates of Hpyloriin the animals of groups Y at the 2nd, 4^th and 6^th week after three challenges were 66.7%,100% and 100%,while in the animals of groups N were 66.7%,66.7% and 100%, respectively. In animals with positive isolation of Hpylori, the bacterium was found to colonized on the surface of gastric mucosal cells and in the gastric pits,and the gastric mucosal lamina propria was infiltrated with inflammatory cells.CONCLUSION:By using H pylori suspension at high concentration of 2×10^9 CFU/mL for multiple times,the orally challenged Mongolian gerbils can be used as a stable and reliable H pylori infection model.The 2 strains of H pylori can colonize in gastric mucosa of the infected animals and cause mild inflammation reaction. | JieYan Yi-HuiLuo Ya-FeiMao | 2004 | World Journal of Gastroenterology2004，10，6： | 3 |
| 10 | Effects of lactose as an inducer on expression of Helicobacter pylori rUreB and rHpaA, and Escherichia colirLTKA63 and rLTB显示文摘AIM: To demonstrate the effect of lactose as an inducer on expression of the recombinant proteins encoded by Helicobacter pylori ureB and hpaA, and Escherichia coli LTB and LTKA63 genes and to determine the optimal expression parameters. METHODS: By using SDS-PAGE and BIO-RAD gel image analysis system, the outputs of the target recombinant proteins expressed by pET32a-ureB-E.coliBL21, pET32a-hpaA-E, coliBL21, pET32a-L TKA63-E. coliBL21 and pET32a-LTB-E.coliBL21 were measured when using lactose as inducer at different dosages, original bacterial concentrations, various inducing temperatures and times. The results of the target protein expression induced by lactose were compared to those by isopropyl-β-D-thiogalactoside (IPTG). The proteins were expressed in E.coli. RESULTS: Lactose showed higher efficiency of inducing the expression of rHpaA, rUreB, rLTB and rLTKA63 than IPTG. The expression outputs of the target recombinant proteins induced at 37℃ were remarkably higher than those at 28℃. Other optimal expression parameters for the original bacterial concentrations, dosages of lactose and inducing time were 0.8, 50 g/L and 4 h for rHpaA; 0.8, 100 g/L and 4 h for rLTKA63; 1.2, 100 g/L and 5 h for both rUreB and rLTB, respectively. CONCLUSION: Lactose, a sugar with non-toxicity and low cost, is able to induce the recombinant genes to express the target proteins with higher efficiency than IPTG. The results in this study establish a beneficial foundation for industrial production of Hpylorigenetic engineering vaccine. | JieYan Shou-FengZhao Ya-EeiMao Yi-HuiLuo | 2004 | World Journal of Gastroenterology2004，10，12： | 2 |
| 11 | Construction of a prokaryotic expression system of vacA gene and detection of vacA gene, VacA protein in Helicobacter pylori isolates and ant-VacA antibody in patients' sera显示文摘AIM: To construct a recombinant prokaryotic expression vector inserted with Helicobacter pylori vacA gene and identify the immunity of the expressed recombinant protein, and to determine prevalence of vacA-carrying/VacA expressing Hpylori isolates and seroprevalence of specific ant-VacA antibody in Hpyloriinfected patients. METHODS: Polymerase chain reaction technique was used to amplify complete vacA gene of H pylori istrain NCTC11637 and to detect vacA gene in 109 H pylori isolates. The amplification product of the complete vacA gene was sequenced after T-A cloning. A recombinant expression vector inserted with a complete vacA gene fragment, named as pET32a-vacA, was constructed. Expression of the target recombinant protein VacA (rVacA) was examined by SDSPAGE. Western blot using commercial antibodies against whole cell of Hpyloriand an immunodiffusion assay using self-prepared rabbit anti-rVacA antibody were applied to determine immunoreaction and antigenicity of rVacA. Two ELISA methods were established to detect VacA expression in Hpyloriisolates and the specific anti-VacA antibody in sera from 125 patients infected with Hpylori. RESULTS: In comparison with the reported corresponding sequences, homologies of nucleotide and putative amino acid sequences of the cloned vacA gene were 99.82% and 100%, respectively. The constructed recombinant prokaryotic expression system efficiently produced rVacA, rVacA was able to combine with the commercial antibodies against whole cell of H pylori and to induce the immunized rabbit to produce specific antibody with an immunodiffusion titer of 1:4. All tested H pylori isolates carried vacA gene, but only 66.1% expressed VacA protein. Of the serum samples tested, 42.4% were positive for specific anti-VacA antibody. CONCLUSION: A prokaryotic expression system of H pylori vacA gene was successfully constructed. The expressed rVacA can be used to detect specific anti-VacA antibody in human and to prepare antiserum in animals. The high frequency of vacA gene in H pylori isolates, but with a low frequency of VacA expression and specific anti-VacA antibody in H pylori infected patients implies that VacA is not an ideal antigen for H pylori vaccine. | JieYan Ya-FeiMao | 2004 | World Journal of Gastroenterology2004，10，7： | 1 |
| 12 | 基于网络药理学探讨地黄治疗糖尿病肾病合并抑郁症的作用机制显示文摘目的基于网络药理学预测地黄治疗糖尿病肾病合并抑郁症的分子作用机制。方法利用中医药整合药理学研究平台(TCMIP)和中药系统药理学数据库和分析平台(TCMSP)以及文献检索地黄活性成分,结合Swiss Target Prediction、Pub Chem数据库查找成分靶点;以“糖尿病肾病”“抑郁症”为关键词在治疗目标数据库(TTD)、DisGeNET和Ensembl数据库搜集疾病靶点,使用Venny 2.1.0对疾病-成分靶点进行映射得到潜在靶点;利用基因/蛋白相互作用检索搜查工具(STRING)数据库结合Cytoscape 3.7.2构建蛋白质相互作用(PPI)网络,基于COXPRESdb 7.3数据库搜集关键靶点共表达基因;运用R语言对潜在靶点进行基因本体论(GO)与京都基因与基因组百科全书(KEGG)富集分析。采用Discovery Studio 4.5对靶点-成分对接实现初步验证和评估。结果根据数据库及文献报道,地黄含65种有效化学成分,治疗糖尿病肾病合并抑郁症的相关靶点155个,PPI筛选显示关键靶点有丝氨酸/苏氨酸蛋白激酶1(AKT1)、信号传导与转录激活因子3(STAT3)、白细胞介素-6(IL-6)、丝裂原活化蛋白激酶1(MAPK1)和血管内皮生长因子A(VEGFA)等。GO富集分析主要涉及血脂代谢、蛋白质分泌调节、细胞体内稳态和磷脂酰肌醇3激酶活性等生物过程;KEGG通路富集分析涉及AGE-RAGE信号通路在糖尿病合并症中的作用、胰岛素抵抗(IR)、神经营养因子信号通路、Toll样受体信号通路、松弛素信号通路和表皮生长因子受体酪氨酸激酶抑制剂(EGFR-TKIs)等。分子对接显示靶点与水苏糖、甘露三糖、毛蕊花糖和黑曲霉二糖等成分具有较高亲和力。结论通过网络构建分析,地黄可能通过调控炎症的发生、调节血糖代谢和营养神经等通路治疗糖尿病肾病合并抑郁症。 | 雷星 陈青垚 王小平 徐杰 高亚珍 林巧红 叶祖文 ZHANG Jieyan SI Qin 王芳 | 2022 | Digital Chinese Medicine2022，5，2： | 1 |
| 13 | Clustering-based probability distribution model for monthly residential building electricity consumption analysis显示文摘Electricity is now the major form of energy used in residential buildings and has seen a significant increase in usage over the past decades.One of the main features of electricity use in residential buildings is the diversity of total electricity consumption and use patterns among households.Current models may not be able to simulate and generate electricity use curves or reflect the variations accurately.To fill this gap,this research simulates electricity use curves in residential buildings with a clustering-based probability distribution model.The model extracts feature parameters to represent the electricity use level and patterns and then conducts a two-step cluster analysis to identify the distinctions of both electricity use levels and patterns.Based on the clustering results,probability distributions are fitted for all feature parameters within each sub-cluster.The model is then validated with three validation approaches.Monthly electricity consumption in households of the Jiangsu Province,China,was studied to test the performance of the model.Lastly,this paper discusses the application of this model under different spatial resolutions and analyzes the temporal-relevant model features. | Jieyan Xu Xuyuan Kang Zheng Chen Da Yan Siyue Guo Yuan Jin Tianyi Hao Rongda Jia | 2021 | Building Simulation2021，14，1： | 1 |
| 14 | Fund manager allocation 显示文摘 | Fang Jieyan Kempf A Trapp M | 2014 | Journal of Financial Economics2014，111，3： | 1 |
| 15 | Preference lo- cation-based routing in delay tolerant networks 显示文摘 | LIU Jieyan GONG Haigang ZENG Jiazhi | 2011 | Inter- national Journal of Digital Content Technology and its Ap- plications2011，5，12： | 1 |
| 16 | Expected shortest path routing for social-oriented intermittently con- nected mobile network 显示文摘 | LIU Jieyan LIU Ming GONG Haigang | 2012 | Journal of Convergence Infor- mation Technology2012，7，1： | 1 |
| 17 | An offset compensated and high-gain CMOS currentfeedback op-amp显示文摘 | ASSI A SAWAN M ZHU Jieyan | 1998 | IEEE Trans on Circuits and Systems1998，45，1： | 1 |
| 18 | An offset compensated and high-gain CMOS current-feedback op-amp显示文摘 | Assi A Sawan M Jieyan Zhu | 1998 | IEEE1998，45，1： | 1 |
| 19 | Getting Their Voices heard:Three Cases of Public Participation in Environmental Protection in China显示文摘 | Li Wanxin Liu Jieyan Li Duoduo | 2012 | Journal of Environmental Management2012，98，15： | 1 |
| 20 | Microbial communities present on mooring chain steels with different copper contents and corrosion rates显示文摘Copper has long been utilized as a disinfectant for bacteria,but its impact on microbial communities attached to the steel surface in seawater remains unknown.In the present study,3 mooring chain steels of different copper contents are subjected to a 3-month marine field exposure,and the corrosion rate increases in the order of BR5 steel(without copper) | WU Jiajia GAO Jieyan ZHANG Dun TAN Faqi YIN Jiang WANG Yu SUN Yan LI Ee | 2020 | Journal of Oceanology and Limnology2020，38，2： | 1 |
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