|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Comparative evaluation of immune response after laparoscopical and open total mesorectal excisions with anal sphincter preservation in patients with rectal cancer显示文摘AIM: The study of immune response of open versus laparoscopical total mesorectal excision with anal sphincter preservation in patients with rectal cancer has not been reported yet. The dissected retroperitoneal area that contacts directly with carbon dioxide is extensive in laparoscopic total mesorectal excision with anal sphincter preservation surgery. Tt is important to clarify whether the immune response of laparoscopic total mesorectal excision with anal sphincter preservation (LTME with ASP) in patients with rectal cancer is suppressed more severely than that of open surgery (OTME with ASP). This study was designed to compare the immune functions after laparoscopic and open total mesorectal excision with anal sphincter preservation for rectal cancer.METHODS: This study involved 45 patients undergoing laparoscopic (n=20) and open (n=25) total mesorectal excisions with anal sphincter preservation for rectal cancer.Serum interleukin-2 (IL-2), interleukin-6 (IL-6), tumor necrosis factor α (TNFα) were assayed preoperatively and on days 1 and 5 postoperatively. CD3+ and CD56+ T lymphocyte count, CD3- and CD56+ natural killer cell (NK)count and immunoglobulin (IgG/IgM/IgA) were assayed preoperatively and on day 5 postoperatively. The numbers of CD3+ and CD56+ T lymphocytes and CD3- and CD56+ NK cells were counted using flow cytometry. An enzyme-linked immunosorbent assay (ELISA) was used for IL-2, TL-6 and TNFα determination. And IgG, IgM, and IgA were assayed using immunonephelometry.RESULTS: The demographic data of the two groups had no difference. The preoperative levels of CD3+ and CD56+ T lymphocyte count, CD3- and CD56+ NK count, serum IgG,IgM, IgA, IL-2, IL-6 and TNFα also had no significant difference in the two groups (P>0.05). The CD3+ and CD56+ T lymphocyte counts had no obvious changes after surgery in laparoscopic (d=-0.79±3.83 %) and open (d=0.42±2.09 %)groups. The CD3- and CD56+ NK counts were decreased postoperatively in both laparoscopic (d=-7.23±11.33 %) and open (d=-9.21±13.93 %) groups. The differences of the determined values of serum IgG, IgM and IgA on the fifth day after operation subtracted those before operation were -2.56±2.14 g/L, -252.35±392.94 mg/L, -506.15±912.24 mg/L in laparoscopic group, and -1.81±2.10 g/L, -282.72±356.75mg/L, -252.20±396.28 mg/L in open group, respectively. The levels of IL-2 were decreased after operation in both groups.However, the levels of IL-6 were decreased after laparoscopic surgery (d1=-23.14±263.97 ng/L and d5=-40.08±272.03 ng/L),and increased after open surgery (d1=27.38±129.14 ng/L and d5=21.67±234.31 ng/L). The TNFα levels were not elevated after surgery in both groups. There were no significant differences in the numbers of CD3+ and CD56+ T lymphocytes and CD3- and CD56+ NK cells, the levels of IgG, IgM, IgA,IL-2, IL-6 and TNFα between the two groups (P>0.05).CONCLUSION: There are no differences in immune responses between the patients having laparoscopic total mesorectal excision with anal sphincter preservation and those undergone open surgery for rectal cancer. | Jian-KunHu Zong-GuangZhou Zhi-XinChen Lan-LanWang Yong-YangYu JinLiu BoZhang LiLi YeShu Jia-PingChen | 2003 | World Journal of Gastroenterology2003，9，12： | 23 |
| 2 | Conversion of pure Chinese fir plantation to multi-layered mixed plantation enhances the soil aggregate stability by regulating microbial communities in subtropical China显示文摘Background:Soil aggregates are the basic units of soil structure,and their stability is a key indicator of soil quality and capacity to support ecosystem functions.The impacts of various environmental factors on soil aggregates have been widely studied.However,there remains elusive knowledge on the synergistic effects of changing forest stand structure on soil aggregate stability(SAS),particularly in subtropical China where soil erosion remains a critical issue.Methods:We investigated variations in the components of soil humus(HS),including humic acids(HAs),fulvic acids(FAs),and humins(HMs),under pure Chinese fir(Cunninghamia lanceolata)plantation(PP)and multilayered mixed plantation(MP)comprising C.lanceolata,Castanopsis hystrix,and Michelia hedyosperma.The state of soil aggregate stability,was determined by three separate methods,i.e.,dry-sieving,wet-sieving,and the Le Bissonnais.High-throughput sequencing was used to determine the diversity and composition of microbial communities under PP and MP.We then built partial least squares path models(PLS-PM)for assessing the responses of SAS to the variations in soil microorganisms and HS components.Results:The MP stands had significantly greater SAS(P<0.05),higher content of HAs and more rapid organic matter humification within aggregates,than the PP stands.High-throughput sequencing confirmed that the Pielou andα-diversity index values(Chao1 and Shannon)for fungi were all significantly higher under MP than under PP,while no marked difference was found in bacterialα-diversity between the two plantation types.Moreover,there were markedly greater abundance of three bacterial phyla(Verrucomicrobia,Chloroflexi,and Gemmatimonadetes)and three fungal phyla(Ascomycota,Kickxellomycota,and Glomeromycota),and significantly less abundance of two bacterial phyla(Planctomycetes and Firmicutes)and four fungal phyla(Basidiomycota,Mortierellomycota,Mucoromycota,and Rozellomycota)under MP than under PP.The Chloroflexi and Ascomycota phyla appeared to be the primary drivers of soil aggregate distribution.Our findings revealed that the promotion of SAS under MP was mainly driven by increased soil organic matter(SOM)content,which altered bacterial communities and enhanced fungal diversity,thereby increasing HAs content and the rate of organic matter humification.Conclusions:Considering the combined effects of enhanced soil quality,productivity,and relevant economic costs,introducing broadleaved tree species into Chinese fir plantations can be an effective strategy for stabilizing soil structure against erosion in subtropical China.Our study elucidated the controls on variations of SAS in Chinese fir-dominated plantations and demonstrated the benefit of converting pure Chinese fir plantation to multi-layered mixed plantations in increasing soil structural stability and improving site quality. | Guannv Gao Xueman Huang Haocheng Xu Yi Wang Weijun Shen Wen Zhang Jinliu Yan Xiaoyan Su Shushou Liao Yeming You | 2022 | Forest Ecosystems2022，9，6： | 4 |
| 3 | Phosphorylated CaMKII post‐synaptic binding to NR2B subunits in the anterior cingulate cortex mediates visceral pain in visceral hypersensitive rats显示文摘 | YingLi XuZhang HaiyanLiu ZhijunCao ShengliangChen BingCao JinLiu | 2012 | Journal of Neurochemistry2012，，4： | 1 |
| 4 | Experimental study on the utilization of DIC by ocystis solitaria wittr and its influ- ence on the precipitation of calcium carbonate in karst and non - karst waters 显示文摘 | LIU Yan LIU Zaihua ZHANG Jinliu | 2010 | Carbonates and Evaporites2010，25，1： | 1 |
| 5 | Experimental study on the utilization of DIC by Oocystis solitaria Wittr and its influence on the precipitation of calcium carbonate in karst and non-karst waters显示文摘 | Yan Liu Zaihua Liu Jinliu Zhang | 2010 | Carbonates and Evaporites2010，25，1： | 1 |
| 6 | Stable Inheritance of the Antisense Waxy Gene in Transgenic Rice with Reduced Amylose Level and Improved Quality显示文摘 | Qiaoquan Liu Zongyang Wang Xiuhua Chen Xiuling Cai Shuzhu Tang Hengxiu Yu Jinliu Zhang Menming Hong Minghong Gu | 2003 | Transgenic Research2003，，1： | 1 |
| 7 | Spatial-temporal variations of travertine deposition rates and their controlling factors in Huanglong ravine, China - a world's heritage site 显示文摘 | Zhang Jinliu Wang Haijing Liu Zaihua | 2012 | Applied Geochemistry2012，27，1： | 1 |
| 8 | Stable Inheritance of the Antisense Waxy Gene in Transgenic Rice with Reduced Amylose Level and Improved Quality显示文摘 | Qiaoquan Liu Zongyang Wang Xiuhua Chen Xiuling Cai Shuzhu Tang Hengxiu Yu Jinliu Zhang Menming Hong Minghong Gu | 2003 | Transgenic Research2003，，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员