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| 1 | Asymmetric distribution of cytokinins determines root hydrotropism in Arabidopsis thaliana显示文摘The phenomenon of plant root tips sensing moisture gradient in soil and growing towards higher water potential is designated as root hydrotropism,which is critical for plants to survive when water is a limited factor.Molecular mechanisms regulating such a fundamental process,however,are largely unknown.Here we report our identification that cytokinins are key signaling molecules directing root growth orientation in a hydrostimulation(moisture gradient)condition.Lower water potential side of the root tip shows more cytokinin response relative to the higher water potential side.Consequently,two cytokinin downstream type-A response regulators,ARR16 and ARR17,were found to be up-regulated at the lower water potential side,causing increased cell division in the meristem zone,which allows the root to bend towards higher water potential side.Genetic analyses indicated that various cytokinin biosynthesis and signaling mutants,including the arr16 arr17 double mutant,are significantly less responsive to hydrostimulation.Consistently,treatments with chemical inhibitors interfering with either cytokinin biosynthesis or cell division completely abolished root hydrotropic response.Asymmetrically induced expression of ARR16 or ARR17 effectively led to root bending in both wild-type and miz1,a previously known hydrotropism-defective mutant.These data demonstrate that asymmetric cytokinin distribution is a primary determinant governing root hydrotropism. | Jinke Chang Xiaopeng Li Weihao Fu Jiawen Wang Yueyuan Yong Hongyong Shi Zhaojun Ding Hong Kui Xiaoping Gou Kai He Jia Li | 2019 | Cell Research2019，29，12： | 4 |
| 2 | RGF1-RGI1,a Peptide-Receptor Complex,Regulates Arabidopsis Root Meristem Development via a MAPK Signaling Cascade显示文摘Root growth is maintained by the continuous division of cells in the apical meristem.ROOT MERISTEM GROWTH FACTOR 1(RGF1)is a critical peptide hormone regulating root stem cell niche maintenance.Previous studies discovered that five closely related leucine-rich repeat receptor-like protein kinases(LRRRLKs),named RGF1 INSENSITIVES(RGIs)or RGF1 RECEPTORS(RGFRs),are able to perceive the RGF1 signal and redundantly control root stem cell niche maintenance.RGF1 regulates root meristem activity mainly via two downstream transcription factors,PLETHORA 1(PLT1)and PLT2.Regulatory proteins connecting cell surface RGF1-RGI1 and nuclear PLTs,however,were not identified.Here,we report that the mitogen-activated protein(MAP)kinase kinase 4(MKK4)and MAP kinase 3(MPK3)were coimmunoprecipitated with RGI1-FLAG after Arabidopsis seedlings were treated with RGF1.Genetic and biochemical assays confirmed that MKK4 and MKK5,and their downstream targets MPK3 and MPK6,are essential RG卜dependent regulators of root meristem development.In addition,we found that the MKK4/MKK5-MPK3/MPK6 module functions downstream of YDA,a MAPKKK.Our results demonstrate that RGF1-RGI1 regulate the expression of PLT1/PLT2 via a YDA-MKK4/MKK5-MPK3/MPK6 signaling cascade. | Xiaoting Lu Hongyong Shi Yang Ou Yanwei Cui Jinke Chang Liang Peng Xiaoping Gou Kai He Jia Li | 2020 | Molecular Plant2020，13，11： | 3 |
| 3 | Overexpression of cystic fibrosis transmembrane conductance regulator (CFTR) is associated with human cervical cancer malignancy, progression and prognosis显示文摘 | Xue Peng Zhao Wu Lei Yu Jinke Li Wenming Xu Hsiao Chang Chan Yi Zhang Lina Hu | 2012 | Gynecologic Oncology2012，，： | 1 |
| 4 | Conversion of mouse fibroblasts into oligodendrocyte progenitor-like cells through a chemical approach显示文摘Transplantation of oligodendrocyte progenitor cells (OPCs) is a promising way for treating demyelinating diseases. However, generation of scalable and autologous sources of OPCs has proven difficult. We previously established a chemical condition M9 that could specifically initiate neural program in mouse embryonic fibroblasts. Here we found that M9 could induce the formation of colonies that undergo mesenchymal-to-epithelial transition at the early stage of reprogramming. These colonies may represent unstable and neural lineage-restricted intermediates that have not established a neural stem cell identity. By modulating the culture signaling recapitulating the principle of OPC development, these intermediate cells could be reprogrammed towards OPC fate. The chemical-induced OPC-like cells (ciOPLCs) resemble primary neural stem cell-derived OPCs in terms of their morphology, gene expression, and the ability of self-renewal. Upon differentiation, ciOPLCs could produce functional oligodendrocytes and myelinate the neuron axons in vitro, validating their OPC identity molecularly and functionally. Therefore, our study provides a non-integrating approach to OPC reprogramming that may ultimately provide an avenue to patient-specific cell-based or in situ regenerative therapy. | Chang Liu Xu Hu Yawen Li Wenjie Lu Wenlin Li Nan Cao Saiyong Zhu Jinke Cheng Sheng Ding Mingliang Zhang | 2019 | Journal of Molecular Cell Biology2019，11，6： | 1 |
| 5 | Coaxial nozzle-assisted electrohydrodynamic printing for microscale 3D cell-laden constructs显示文摘Cell printing has found wide applications in biomedical fields due to its unique capability in fabricating living tissue constructs with precise control over cell arrangements.However,it is still challenging to print cell-laden 3D structures simultaneously with high resolution and high cell viability.Here a coaxial nozzle-assisted electrohydrodynamic cell printing strategy was developed to fabricate living 3D cell-laden constructs.Critical process parameters such as feeding rate and stage moving speed were evaluated to achieve smaller hydrogel filaments.The effect of CaCl2 feeding rate on the printing of 3D alginate hydrogel constructs was also investigated.The results indicated that the presented strategy can print 3D hydrogel structures with relatively uniform filament dimension(about 80μm)and cell distribution.The viability of the encapsulated cells was over 90%.We envision that the coaxial nozzle-assisted electrohydrodynamic printing will become a promising cell printing strategy to advance biomedical innovations. | Hongtao Liang Jiankang He Jinke Chang Bing Zhang Dichen Li | 2018 | International Journal of Bioprinting2018，4，1： | 0 |
| 6 | Refractive Index Measurement and Second Harmonic Generation in a Series of LiNbO_(3):Mg(5mol%)Crystals显示文摘The accurate refractive indices in visible range and their Sellmeier equations have been obtained for a series of LiNbO_(3):Mg(5mol%)crystals with Ti or Nd doping.The phase matching angle for 1.064μm calculated by Sellmeier equation gives good agreement with experimental value.The TiO_(2) and Nd2O_(3) doping cause the decrease of the birefringence and the increase of the phase matching angle.The conversion efficiency is enhanced by TiO_(2) doping.The second harmonic generator with conversion efficiency of more than 44%has been achieved at room temperature with phase matching angle close to 90°. | CHANG Yongmao WEN Jinke WANG Huafu LI Bing | 1992 | Chinese Physics Letters1992，9，8： | 0 |
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