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| 1 | Generation of new glutinous rice by CRISPR/Cas9-targeted mutagenesis of the Waxy gene in elite rice varieties显示文摘In rice, amylose content(AC) is controlled by a single dominant Waxy gene. We used Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)/CRISPR-associated 9(Cas_9) to introduce a loss-of-function mutation into the Waxy gene in two widely cultivated elite japonica varieties. Our results show that mutations in the Waxy gene reduce AC and convert the rice into glutinous ones without affecting other desirable agronomic traits, offering an effective and easy strategy to improve glutinosity in elite varieties. Importantly, we successfully removed the transgenes from the progeny. Our study provides an example of generating improved crops with potential for commercialization, by editing a gene of interest directly in elite crop varieties. | Jinshan Zhang Hui Zhang Jose Ramón Botella Jian-Kang Zhu | 2018 | Journal of Integrative Plant Biology2018，60，5： | 49 |
| 2 | Shortened Basal Internodes Encodes a Gibberellin 2-Oxidase and Contributes to Lodging Resistance in Rice显示文摘改进住宿抵抗的繁殖半矮子变化被证明了在自从绿 revolution.?However 的来临,增加谷物产量极其成功,在亚洲繁殖半矮子米饭变化的多数主要依赖于 SD1 的变异的等位基因的基因介绍,它编码赤霉素( GA ) 20-oxidase , OsGA20ox2 ,为催化 GA 生合成。这里,我们报导新米饭住宿抵抗基因,弄短的基础 Internodes (SBI ) ,它编码赤霉素 ? 2-oxidase 并且明确地控制灰煤杆的延伸通过撤销的基础 internodes GA 活动。SBI 主要在灰煤杆被表示基础 internodes。基因分析显示 SBI 是影响米饭高度和住宿抵抗的半主导的基因。SBI 突变而产生之遗传的变体显示不同活动并且与米饭变化的高度被联系。与 SBI 等位基因的更高的活动繁殖与改进住宿抵抗和增加的产量产生新米饭变化。SBI 的发现为生产半矮子米饭显型提供一个理想的基因资源并且为与提高的住宿抵抗和高产量引起米饭变化提供有效策略。 | Chang Liu Jinshan Gui Chenjian Fu Hasi Yu Dongliang Song Junhui Shen Peng Qin Xuanming Liu Bin Han Yuanzhu Yang Laigeng Li | 2018 | Molecular Plant2018，11，2： | 33 |
| 3 | Perspectives on the Application of Genome-Editing Technologies in Crop Breeding显示文摘Most conventional and modern crop-improvement methods exploit natural or artificially induced genetic variations and require laborious characterization of the progenies of multiple generations derived from time-consuming genetic crosses.Genome-editing systems,in contrast,provide the means to rapidly modify genomes in a precise and predictable way,making it possible to introduce improvements directly into elite varieties.Here,we describe the range of applications available to agricultural researchers using existing genome-editing tools.In addition to providing examples of genome-editing applications in crop breeding,we discuss the technical and social challenges faced by breeders using genome-editing tools for crop improvement. | Kai Hua Jinshan Zhang Jose Ramon Botella Changle Ma Fanjiang Kong Baohui Liu Jian-Kang Zhu | 2019 | Molecular Plant2019，12，8： | 19 |
| 4 | Sparse SAR imaging based on L_(1/2) regularization显示文摘In this paper,a novel method for synthetic aperture radar(SAR)imaging is proposed.The approach is based on L1/2 regularization to reconstruct the scattering field,which optimizes a quadratic error term of the SAR observation process subject to the interested scene sparsity.Compared to the conventional SAR imaging technique,the new method implements SAR imaging effectively at much lower sampling rate than the Nyquist rate,and produces high-quality images with reduced sidelobes and increased resolution.Also,over the prevalent greedy pursuit and L1 regularization based SAR imaging methods,there are remarkable performance improvements of the new method.On one hand,the new method significantly reduces the number of measurements needed for reconstruction,as supported by a phase transition diagram study.On the other hand,the new method is more robust to the observation noise.These fundamental properties of the new method are supported and demonstrated both by simulations and real SAR data experiments. | ZENG JinShan FANG Jian XU ZongBen | 2012 | Science China(Information Sciences)2012，55，8： | 18 |
| 5 | Corrosion Behavior of Mg-Zn-Y Alloy with Long-period Stacking Ordered Structures显示文摘Mg-Zn-Y alloys with long-period stacking ordered structures were prepared by an ingot casting method. The corrosion performance of Mg-Zn-Y alloys was studied by combining gas-collecting test, immersion test and electrochemical measurements in order to determine the corrosion rate and mechanism of the alloys. The results showed that the volume fraction of Mg(12)YZn phase increased and the shape of the Mg(12)YZn phase changed from discontinuous to continuous net-like with increasing Zn and Y content. The corrosion rate of the alloys greatly depended on the distribution and volume fraction of the Mg(12)YZn phase. Corrosion products appeared at the junction of Mg phase and Mg(12)YZn phase, indicating that the Mg(12)YZn phase accelerated galvanic corrosion of Mg matrix. Mg(97)Zn1Y2 alloy shows the lowest corrosion rate due to the continuous distribution of Mg(12)YZn phase. | Jinshan Zhang Jidong Xu Weili Cheng Changjiu Chen Jingjing Kang | 2012 | Journal of Materials Science & Technology2012，28，12： | 17 |
| 6 | Effects of applying lactic acid bacteria to the fermentation on a mixture of corn steep liquor and air-dried rice straw显示文摘This study was to determine the fermentation quality of a mixture of corn steep liquor(CSL)(178 g/kg wet basis) and air-dried rice straw(356 g/kg wet basis) after being treated with inoculants of different types of lactic acid bacteria(LAB). The treatments included the addition of no LAB additive(control),which was deionized water; homo-fermentative LAB alone(^(ho)LAB), which was Lactobacillus plantarum alone), and a mixture of homo-fermentative and hetero-fermentative LAB(^(he+ho)LAB), which were L. plantarum, Lactobacillus casei, and Lactobacillus buchneri. The results showed that the inoculation of the mixture of CSL and air-dried rice straw with ^(he+ho)LAB significantly increased the concentration of acetic acid and lactic acid compared with the control(P < 0.05). The addition of ^(he+ho)LAB effectively inhibited the growth of yeast in the silage. The concentration of total lactic acid bacteria in the ^(he+ho)LAB-treated silage was significant higher than those obtained in other groups(P < 0.05). The duration of the aerobic stability of the silages increased from 56 h to >372 h. The control group was the first to spoil, whereas the silage treated with ^(he+ho)LAB remained stable throughout the 372 h period of monitoring. The results demonstrated that the ^(he+ho)LAB could effectively improve the fermentation quality and aerobic stability of the silage. | Xinxin Li Wenbin Xu Jinshan Yang Hongbo Zhao Chunfang Pan Xue Ding Yonggen Zhang | 2016 | Animal Nutrition2016，，3： | 17 |
| 7 | Position and complications of pedicle screw insertion with or without image-navigation techniques in the thoracolumbar spine:a meta-analysis of comparative studies显示文摘Computer-navigated pedicle screw insertion is applied to the thoracic and lumbar spine to attain high insertion accuracy and a low rate of screw-related complications.However,some in vivo and in vitro studies have shown that no advantages are gained with the use of navigation techniques compared to conventional techniques.Additionally,inconsistent conclusions have been drawn in various studies due to different population characteristics and methods used to assess the accuracy of screw placement.Moreover,it is not clear whether pedicle screw insertion with navigation techniques decreases the incidence of screw-related complications.Therefore,this study was sought to perform a meta-analysis of all available prospective evidence regarding pedicle screw insertion with or without navigation techniques in human thoracic and lumbar spine.We considered in vivo comparative studies that assessed the results of pedicle screw placement with or without navigation techniques.PubMed,Ovid MEDLINE and EMBASE databases were searched.Three published randomized controlled trials(RCTs) and nine retrospective comparative studies met the inclusion criteria.These studies included a total of 732 patients in whom 4,953 screws were inserted.In conclusion,accuracy of the position of grade I,II,III and IV screws and complication rate related to pedicle screw placement were significantly increased when navigation techniques were used in comparison to conventional techniques.Future research in this area should include RCTs with well-planned methodology to limit bias and report on validated,patient-based outcome measures. | Jinshan Tang Ziqiang Zhu Tao Sui Dechao Kong Xiaojian Cao | 2014 | The Journal of Biomedical Research2014，28，3： | 15 |
| 8 | Microstructural evolution and FCC twinning behavior during hot deformation of high temperature titanium alloy Ti65显示文摘Although the development of titanium alloys with working temperatures above 600?C faces enormous difficulties and challenges,the related research has not stopped.In the present work,detailed analyses on microstructure evolution and hot deformation behavior of a new temperature resistant 650?C titanium alloy Ti65 were investigated from micrometer scale to nanometer scale.The results revealed that lamellarαgrains gradually fragmentized and spheroidized during theα+βphase region compression and the orientation of the c-axis ofαgrains gradually aligned to radial directions,forming two high Schmid factors(SFs)value texture eventually with the increase of strain to 0.7.Moreover,there were some strengthening characters in theα+βphase region such as lenticularαsand nano silicide(TiZr)6 Si3.In theβphase region,fine equiaxed dynamic recrystallized(DRX)βgrains were formed.Besides,the variant selection ofαm′artensite followed Burgers orientation relationship during the compression process.The main deformation mechanisms of theα+βphase region were dislocation slip and orientation dependent spheroidization.Whereas,the deformation process in theβphase region was controlled byβgrain DRX.Interestingly,many nano scale FCC twins were generated at the interface ofαl′ath during deforming in theβphase region,which was firstly observed in Ti65 alloy. | Zhixin Zhang Jiangkun Fan Bin Tang Hongchao Kou Jian Wang Xin Wang Shiying Wang Qingjiang Wang Zhiyong Chen Jinshan Li | 2020 | Journal of Materials Science & Technology2020，47，14： | 10 |
| 9 | Phosphorylation of LTF1, an MYB Transcription Factor in Populus, Acts as a Sensory Switch Regulating Lignin Biosynthesis in Wood Cells显示文摘Lignin is specifically deposited in plant secondary cell walls,and initiation of lignin biosynthesis is regulated by a variety of developmental and environmental signals.However,the mechanisms governing the regulation of lignin biosynthesis remain to be elucidated.In this study,we identified a lignin biosynthesis-associated transcription factor(LTF)from Populus,LTF1,which binds the promoter of a key lignin biosynthetic gene encoding 4-coumarate-CoA ligase(4CL).We showed that LTF1 in its unphosphorylated state functions as a regulator restraining lignin biosynthesis.When LTF1 becomes phosphorylated by PdMPK6 in response to external stimuli such as wounding,it undergoes degradation through a proteasome pathway,resulting in activation of lignification.Expression of a phosphorylation-null mutant version of LTF1 led to stable protein accumulation and persistent attenuation of lignification in wood cells.Taken together,our study reveals a mechanism whereby LTF1 phosphorylation acts as a sensory switch to regulate lignin biosynthesis in response to environmental stimuli.The discovery of novel modulators and mechanisms modifying lignin biosynthesis has important implications for improving the utilization of cell-wall biomass. | Jinshan Gui Laifu Luo Yu Zhong Jiayan Sun Toshiaki Umezawa Laigeng Li | 2019 | Molecular Plant2019，12，10： | 10 |
| 10 | Numerical Investigations on the Steady and Unsteady Leakage Flow and Heat Transfer Characteristics of Rotor Blade Squealer Tip显示文摘The steady and unsteady leakage flow and heat transfer characteristics of the rotor blade squealer tip were conducted by solving Reynolds-Averaged Navier-Stokes(RANS) equations with k-ω turbulence model.The first stage of GE-E3 engine with squealer tip in the rotor was adopted to perform this work.The tip clearance was set to be 1% of the rotor blade height and the groove depth was specified as 2% of the span.The results showed that there were two vortexes in the tip gap which determined the local heat transfer characteristics.In the steady flow field,the high heat transfer coefficient existed at several positions.In the unsteady case,the flow field in the squealer tip was mainly influenced by the upstream wake and the interaction of the blades potential fields.These unsteady effects induced the periodic variation of the leakage flow and the vortexes,which resulted in the fluctuation of the heat transfer coefficient.The largest fluctuation of the heat transfer coefficient on the surface of the groove bottom exceeded 16% of the averaged value on the surface of the squealer tip. | Jun LI Hao SUN Jinshan WANG Zhenping FENG | 2011 | Journal of Thermal Science2011，20，4： | 9 |
| 11 | Layered double hydroxide(LDH) for multi-functionalized corrosion protection of metals: A review显示文摘Layered double hydroxide(LDH) has been widely developed in the field of corrosion and protection in recent years based on its unique characteristics including anion capacity, anion exchange ability, structure memory effect, and barrier resistance. This paper comprehensively reviews recent work on the preparations, properties of LDH in the forms of powder and film and their applications in different environments in corrosion and protection. Some novel perspectives are also proposed at the end of the review for future research in corrosion and protection field. | Yanhui Cao Dajiang Zheng Fan Zhang Jinshan Pan Changjian Lin | 2022 | Journal of Materials Science & Technology2022，，7： | 9 |
| 12 | Control of Surface Thermal Scratch of Strip in Tandem Cold Rolling显示文摘The thermal scratch seriously affects the surface quality of the cold rolled stainless steel strip. Some researchers have carried out qualitative and theoretical studies in this field. However, there is currently a lack of research on effective forecast and control of thermal scratch defects in practical production, especially in tandem cold rolling. In order to establish precise mathematical model of oil film thickness in deformation zone, the lubrication in cold rolling process of SUS410L stainless steel strip is studied, and major factors affecting oil film thickness are also analyzed. According to the principle of statistics, mathematical model of critical oil film thickness in deformation zone for thermal scratch is built, with fitting and regression analytical method, and then based on temperature comparison method, the criterion for deciding thermal scratch defects is put forward. Storing and calling data through SQL Server 2010, a software on thermal scratch defects control is developed through Microsoft Visual Studio 2008 by MFC technique for stainless steel in tandem cold rolling, and then it is put into practical production. Statistics indicate that the hit rate of thermal scratch is as high as 92.38%, and the occurrence rate of thermal scratch is decreased by 89.13%. Owing to the application of the software, the rolling speed is increased by approximately 9.3%. The software developed provides an effective solution to the problem of thermal scratch defects in tandem cold rolling, and helps to promote products surface quality of stainless steel strips in practical production. | CHEN Jinshan LI Changsheng | 2014 | Chinese Journal of Mechanical Engineering2014，27，4： | 8 |
| 13 | Research on KOH/La-Ba-Al_2O_3 catalysts for biodiesel production via transesterification from microalgae oil显示文摘Alumina supports modified by lanthanum(La)and barium(Ba)were prepared by peptization.Catalysts with different KOH contents supported on modified alumina were prepared by impregnation method.Various techniques,including N2 adsorption-desorption(Brunauer-Emmet-Teller method,BET),X-ray diffraction(XRD),scanning electron microscopy(SEM),and fourier transform infrared absorption spectroscopy(FT-IR). Catalytic activity for microalgae oil conversion to methyl ester via transesterification was evaluated and analyzed by GC-MS and GC.BET results showed that the support possessed high specific surface area,suitable pore volume and pore size distribution.Activity results indicated that the catalyst with 25 wt%KOH showed the best activity for microalgae oil conversion.XRD and SEM results revealed that Al-O-K compound was the active phase for microalgae oil conversion.The agglomeration and changing of pore structure should be the main reasons for the catalyst deactivation when KOH content was higher than 30 wt%. | Xiaoyu Zhang Qing Ma Bibo Cheng Jun Wang Jinshan Li Fude Nie | 2012 | Journal of Natural Gas Chemistry2012，21，6： | 7 |
| 14 | Orientation dependent behavior of tensile-creep deformation of hot rolled Ti65 titanium alloy sheet显示文摘The mill products like sheet always have one or more severe textures inevitably,and its effect on mechanical properties is not a negligible issue.The orientation dependent tensile-creep behavior induced by rolling texture of Ti65 titanium alloy sheet has been systematically investigated at 650℃.There are some anisotropic characteristics between TD and RD of Ti65 sheet.The UTS and TYS of TD are higher than RD at 650℃.Besides,the creep endurance time of TD(172.6–174.5 h)is about three times longer than RD(55.6–65.1 h)at 650℃and 240 MPa.Moreover,the grains are inclined to form Texture III■after creep along with TD,but to form Texture I■after creep along with RD.Finally,the crack initiation site is different during creep in TD and RD.The reason for anisotropic properties of tensile and creep has been summarized in two aspects:(i)the change of the SFs(Schmid factors)value between TD and RD;(ii)the difference of creep mechanism between TD(grain boundary sliding)and RD(dislocation slip).Anisotropy of Ti65 sheet should be fully considered to increase structural efficiency in the engineering design and application. | Zhixin Zhang Jiangkun Fan Ruifeng Li Hongchao Kou Zhiyong Chen Qingjiang Wang Hailong Zhang Jian Wang Qi Gao Jinshan Li | 2021 | Journal of Materials Science & Technology2021，，16： | 7 |
| 15 | A Resource for Inactivation of MicroRNAs Using Short Tandem Target Mimic Technology in Model and Crop Plants显示文摘microRNAs (miRNAs)are endogenous small non-coding RNAs that bind to mRNAs and target them for cleavage and/or translational repression,leading to gene silencing.We previously developed short tandem target mimic (STTM)technology to deactivate endogenous miRNAs in Arabidopsis.Here,we created hundreds of STTMs that target both conserved and species-specific miRNAs in Arabidopsis,tomato,rice,and maize,providing a resource for the functional interrogation of miRNAs.We not only revealed the functions of several miRNAs in plant development,but also demonstrated that tissue-specific inactivation of a few miRNAs in rice leads to an increase in grain size without adversely affecting overall plant growth and development.RNA-seq and small RNAseq analyses of STTM156/157 and STTM165/166 transgenic plants revealed the roles of these miRNAs in plant hormone biosynthesis and activation,secondary metabolism,and ion-channel activity-associated electrophysiology,demonstrating that STTM technology is an effective approach for studying miRNA functions.To facilitate the study and application of STTM transgenic plants and to provide a useful platform for storing and sharing of information about miRNA-regulated gene networks,we have established an online Genome Browser (https://blossom.ffr.mtu.edu/designindex2.php) to display the transcriptomic and miRNAomic changes in STTMinduced miRNA knockdown plants. | Ting Peng Mengmeng Qiao Haiping Liu Sachin Teotia Zhanhui Zhang Yafan Zhao Bobo Wang Dongjie Zhao Lina Shi Cui Zhang Brandon Le Kestrel Rogers Chathura Gunasekara Haitang Duan Yiyou Gu Lei Tian Jinfu Nie Jian Qi Fanrong Meng Lan Huang Qinghui Chen Zhenlin Wang Jinshan Tang Xiaoqing Tang Ting Lan Xuemei Chen Hairong Wei Quanzhi Zhao Guiliang Tang | 2018 | Molecular Plant2018，11，11： | 7 |
| 16 | 3D finite element simulation of explosive welding of three-layer plates显示文摘A 3D finite element model of the explosive welding process of three-layer plates with materials of steel-copper-copper is established. Based on the presented model, the bonding mechanism is simulated and analyzed, different detonation modes are also comparatively studied to indicate the driving force spread in few microseconds. The results show that the three layer plates bond together after many times of impact between the flyers and the base driven by detonation wave, which is damping rapidly at each impact with wavelength decreasing. The pressure at the detonation point is minimal, which induces non-bonding of the plates here. Detonation wave propagates in concentric circle both under side-midpoint detonation mode and under center-point detonation mode, but the movement of the flyer is different, which makes non-bonding easily occur at the end of detonation under side-midpoint detonation and at the center of the plate under center-point detonation. | SUI GuoFa LI JinShan SUN Feng MA Bei LI HongWei | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，5： | 7 |
| 17 | Natural selection maintains the transcribed LTR retrotransposons in Nosema bombycis显示文摘Eight intact LTR retrotransposons(Nbr1?Nbr8)have been previously characterized from the genome of Nosema bombycis,a eu-karyotic parasite with a compact and reduced genome.Here we describe six novel transcribed Nbr elements(Nbr9?Nbr14)identified through either cDNA library or RT-PCR.Like previously determined ones,all of them belong to the Ty3/Gypsy superfamily.Retrotransposon diversity and incomplete domains with insertions(Nbr12),deletions(Nbr11)and in-frame stop codons in coding regions(Nbr9)were detected,suggesting that both defective and loss events of LTR retrotransposon have happened in N.bombycis genome.Analysis of selection showed that strong purifying selection acts on all elements except Nbr11.This implies that selective pressure keeps both these Nbrs and their functions in genome.Interestingly,Nbr11 is under positive selection and some positively selected codons were identified,indicating that new functionality might have evolved in the Nbr11 retrotransposon.Unlike other transposable elements,Nbr11 has integrated into a conserved syntenic block and probably resulted in the inversion of both flanking regions.This demonstrates that transposable element is an important factor for the reshuffling and evolution of their host genomes,and may be maintained under natural selection. | Heng Xiang Guoqing Pan Ruizhi Zhang Jinshan Xu Tian Li Wenle Li Zeyang Zhou Zhonghuai Xiang | 2010 | Journal of Genetics and Genomics2010，37，5： | 7 |
| 18 | Disruption of MIR396e and MIR396f improves rice yield under nitrogen-deficient conditions显示文摘The microRNA miR396 directly represses GROWTH-REGULATING FACTORs(OsGRFs)and has been implicated in regulating rice yield and in nitrogen assimilation.Overexpressing the miR396 targets OsGRF4 and OsGRF6 improves rice yield via increased grain size and panicle branching,respectively.Here,we used CRISPR/Cas9 to assess the function of miR396 genes in rice.Knockout of MIR396 ef(MIR396e and MIR396f),but not other isoforms,enhanced both grain size and panicle branching,resulting in increased grain yield.Importantly,under nitrogen-deficient conditions,mir396ef mutants showed an even higher relative increase in grain yield as well as elevated above-ground biomass.Furthermore,we identified OsGRF8 as a new target of miR396,in addition to the known targets OsGRF4 and OsGRF6.Disruption of the miR396-targeting site in OsGRF8 was sufficient to both enlarge grain size and elongate panicles.Our results suggest that rice-seed and panicle development are regulated by miR396ef-GRF4/6/8-GIF1/2/3 modules and that mi R396ef are promising targets of genome editing for breeding environmentally friendly rice varieties that require less nitrogen fertilization. | Jinshan Zhang Zhenyu Zhou Jinjuan Bai Xiaoping Tao Ling Wang Hui Zhang Jian-Kang Zhu | 2020 | National Science Review2020，7，1： | 7 |
| 19 | Rhizosphere and bulk soil enzyme activities in a Nothotsuga longibracteata forest in the Tianbaoyan National Nature Reserve,Fujian Province,China显示文摘The rhizosphere, distinct from bulk soil, is defined as the volume of soil around living roots and influenced by root activities. We investigated protease,invertase, cellulase, urease, and acid phosphatase activities in rhizosphere and bulk soils of six Nothotsuga longibracteata forest communities within Tianbaoyan National Nature Reserve, including N. longibracteata + either Phyllostachys pubescens, Schima superba, Rhododendron simiarum, Cunninghamia lanceolata, or Cyclobalanopsis glauca, and N. longibracteata pure forest. Rhizosphere soils possessed higher protease, invertase, cellulase, urease,and acid phosphatase activities than bulk soils. The highest invertase, urease, and acid phosphatase activities were observed in rhizosphere samples of N. longibracteata+ S.superba. Protease was highest in the N. longibracteata + R. simiarum rhizosphere, while cellulase was highest in the pure N. longibracteata forest rhizosphere.All samples exhibited obvious rhizosphere effects on enzyme activities with a significant linear correlation between acid phosphatase and cellulase activities(p \ 0.05) in rhizosphere soils and between protease and acid phosphatase activities(p \ 0.05) in bulk soils. A principal component analysis, correlating 13 soil chemical properties indices relevant to enzyme activities, showed that protease, invertase, acid phosphatase, total N, and cellulase were the most important variables impacting rhizosphere soil quality. | Shihong Xiao Huiming You Weibin You Jinshan Liu Changtang Cai Jianqin Wu Zhirong Ji Shihua Zhan Zhesen Hu Zhongrui Zhang Dongjin He | 2017 | Journal of Forestry Research2017，28，3： | 7 |
| 20 | Effect of different levels of corn steep liquor addition on fermentation characteristics and aerobic stability of fresh rice straw silage显示文摘The object of this study was to determine the proper mixing ratio of fresh rice straw to corn steep liquor(CSL) to obtain a high protein content silage feed. The following experimental silages were generated: the control(C1), composed of fresh rice straw without CSL additive, mixed with CSL in the ratios of 4:1(C4),3:1(C3) and 2:1(C2). Lactic acid bacteria(LAB) inoculant was applied at the rate of 50 mL/kg(fresh basis)of forage to achieve a final application rate of 1 x 10~6 cfu/g of fresh matter(FM). Duplicate silos for each treatment were opened after 0,3, 7,10,20,30,45 and 60 d for microbiological and chemical analysis. The results showed that the addition of CSL significantly increased crude protein(CP) contents, and decreased neutral detergent fiber(NDF) and acid detergent fiber(ADF) contents of treatments after 60 d of ensiling(P < 0.05). The lactic acid contents in C4 and C3 were significantly higher than that in C1(P <0.05). In summary, mixing fresh rice straw with CSL at addition levels of 4:1(C4) and 3:1(C3) can improve the fermentation quality and nutrient composition of fresh rice straw silage. However, a large proportion of CSL(C3) had a negative impact on the aerobic stability of fresh rice straw. | Xinxin Li Wenbin Xu Jinshan Yang Hongbo Zhao Hangshu Xin Yonggen Zhang | 2016 | Animal Nutrition2016，，4： | 6 |
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