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| 1 | Versatile Nucleotides Substitution in Plant Using an Improved Prime Editing System显示文摘Dear Editor,Base editors(BEs)based on the CRISPR/Cas9 system,including cytosine base editors and adenine base editors,which can efficiently perform four transition mutations(C·G-to-T·A and A·T-to-G·C),have been well studied and widely used to produce base mutations in a variety of organisms,including in plants such as rice. | Wen Xu Chengwei Zhang Yongxing Yang Si Zhao Guiting Kang Xiaoqing He Jinling Song Jinxiao Yang | 2020 | Molecular Plant2020，13，5： | 20 |
| 2 | CAS FGOALS-f3-L Model Datasets for CMIP6 Historical Atmospheric Model Intercomparison Project Simulation显示文摘The outputs of the Chinese Academy of Sciences(CAS) Flexible Global Ocean–Atmosphere–Land System(FGOALS-f3-L) model for the baseline experiment of the Atmospheric Model Intercomparison Project simulation in the Diagnostic,Evaluation and Characterization of Klima common experiments of phase 6 of the Coupled Model Intercomparison Project(CMIP6) are described in this paper. The CAS FGOALS-f3-L model, experiment settings, and outputs are all given. In total,there are three ensemble experiments over the period 1979–2014, which are performed with different initial states. The model outputs contain a total of 37 variables and include the required three-hourly mean, six-hourly transient, daily and monthly mean datasets. The baseline performances of the model are validated at different time scales. The preliminary evaluation suggests that the CAS FGOALS-f3-L model can capture the basic patterns of atmospheric circulation and precipitation well, including the propagation of the Madden–Julian Oscillation, activities of tropical cyclones, and the characterization of extreme precipitation. These datasets contribute to the benchmark of current model behaviors for the desired continuity of CMIP. | Bian HE Qing BAO Xiaocong WANG Linjiong ZHOU Xiaofei WU Yimin LIU Guoxiong WU Kangjun CHEN Sicheng HE Wenting HU Jiandong LI Jinxiao LI Guokui NIAN Lei WANG Jing YANG Minghua ZHANG Xiaoqi ZHANG | 2019 | Advances in Atmospheric Sciences2019，36，8： | 12 |
| 3 | Discriminated sgRNAs-Based SurroGate System Greatly Enhances the Screening Efficiency of Plant Base-Edited Cells显示文摘The development of CRISPR/Cas9-mediated base editing has made genomic modification more efficient. However, selection of genetically modified cells from millions of treated cells, especially plant cells, is still challenging. In this study, an efficient surrogate reporter system based on a defective hygromycin resistance gene was established in rice to enrich base-edited cells. After step-by-step optimization, the Discriminated sgRNAs-based SurroGate system (DisSUGs) was established by artificially differentiating the editing abilities of a wild-type single guide RNA (sgRNA) targeting the surrogate reporter gene and an enhanced sgRNA targeting endogenous sites. The DisSUGs enhanced the efficiency of screening base-edited cells by 3- to 5-fold for a PmCDA1-based cytosine-to-tyrosine base editor (PCBE), and 2.5- to 6.5-fold for an adenine base editor (ABE) at endogenous targets. These targets showed editing efficiencies of <25% in the conventional systems. The DisSUGs greatly enhanced the frequency of homozygous substitutions and expanded the activity window slightly for both a PCBE and an ABE. Analyses of the total number of single-nucleotide variants from whole-genome sequencing revealed that, compared with the no-enrichment PCBE strategy, the DisSUGs did not alter the frequency of genome-wide sgRNA-independent off-target mutations, but slightly increased the frequency of target-dependent off-target mutations. Collectively, the DisSUGs developed in this study greatly enhances the efficiency of screening plant base-edited cells and will be a useful system in future applications. | Wen Xu Yongxing Yang Ya Liu Guiting Kang Feipeng Wang Lu Li Xinxin Lv Si Zhao Shuang Yuan Jinling Song Ying Wu Feng Feng Xiaoqing He Chengwei Zhang Wei Song Jiuran Zhao Jinxiao Yang | 2020 | Molecular Plant2020，13，1： | 8 |
| 4 | Integrated metabolo-transcriptomics and functional characterization reveals that the wheat auxin receptor TIR1 negatively regulates defense against Fusarium graminearum显示文摘Fusarium head blight(FHB) caused by Fusarium graminearum Schwabe(teleomorph Gibberella zeae(Schw.) Perch) results in large yield losses in annual global wheat production. Although studies have identified a number of wheat FHB resistance genes,a deeper understanding of the mechanisms underlying host plant resistance to F. graminearum is required for the control of FHB. Here, an integrated metabolomics and transcriptomics analysis of infected wheat plants(Triticum aestivum L.) enabled identification of 789 differentially accumulated metabolites, including flavonoids, phenolamides, tryptamine derivatives, and phytohormones, and revealed altered expression of more than 100 genes that function in the biosynthesis or regulation of these pathways. Our data regarding the effects of F. graminearum infection on flavonoids and auxin signaling led to follow-up experiments that showed that exogenous kaempferide and apigenin application on spikes increased wheat resistance to FHB,while exogenous auxin treatment increased FHB susceptibility. RNAi-mediated knockdown of the gene encoding the auxin receptor, Ta TIR1, increased FHB resistance. Our data supported the use of Ta TIR1 knockdown in controlling FHB. Our study provides insights on the wheat response to F.graminearum infection and its FHB resistance mechanisms while illustrating the potential of Ta TIR1 knockdown in increasing FHB resistance during crop improvement programs. | Peisen Su Lanfei Zhao Wen Li Jinxiao Zhao Jun Yan Xin Ma Anfei Li Hongwei Wang Lingrang Kong | 2021 | Journal of Integrative Plant Biology2021，63，2： | 6 |
| 5 | Effect of pickling processes on the microstructure and properties of electroless Ni–P coating on Mg–7.5Li–2Zn–1Y alloy显示文摘The electroless plating Ni–P is prepared on the surface of Mg–7.5Li–2Zn–1Y alloys with different pickling processes.The microstructure and properties of Ni–P coating are investigated.The results show that the Ni–P coatings deposited using the different pickling processes have a different high phosphorus content amorphous Ni–P solid solution structure,and the Ni–P coatings exhibit higher hardness.There is higher phosphorus content of Ni–P amorphous coating using 125 g/L Cr O3and 110 ml/L HNO3(w68%)than using 180 g/L Cr O3and 1 g/L KF during pre-treatment,and the coating structure is more compact,and the Ni–P coatings exhibit more excellent adhesion with substrate(Fcup to22 N).The corrosion potential of Ni–P coating is improved and exhibits good corrosion resistance.As a result,Mg-7.5Li-2Zn-1Y alloy is remarkably protected by the Ni–P coating. | Chunjie Xu Liping Chen Ling Yu Jinxiao Zhang Zhongming Zhang Jincheng Wang | 2014 | Progress in Natural Science:Materials International2014，24，6： | 5 |
| 6 | Expanding base editing scope to near-PAMless with engineered CRISPR/Cas9 variants in plants显示文摘Dear Editor,Base editors(BEs),including cytosine base editor(CBE)and adenine base editor(ABE),have been widely used to generate irreversible nucleotide substitution in plants and animals.However,their wide applications are largely hindered by the strict NG protospacer adjacent motif(PAM)sequences recognized by Streptococcus pyogenes Cas9(SpCas9)and its engineered variants,such as SpCas9-NG and xCas9(Hua et al.,2019;Ren et al.,2019;Wu et al.,2019;Zhong et al.,2019;Zhang et al.,2020).Most recently,it was reported that three new SpCas9 variants,SpCas9-NRRH,SpCas9-NRTH,and SpCas9-NRCH,could recognize non-G PAMs(NRNH,where R is A or G and H is A,C,or T)in human cells(Miller et al.,2020).Meanwhile,SPRY,another new SpCas9 variant,was developed to greatly expand the editing scope of BEs to nearly PAMless(Walton et al.,2020).In this study,we generated a series of efficient BE toolkits and almost achieved C-to-T mutation without PAM restriction except for NTG PAM,and largely expanded A-to-G mutation scope in stable transformed rice,providing a reference for application in other plants. | Chengwei Zhang Yao Wang Feipeng Wang Si Zhao Jinling Song Feng Feng Jiuran Zhao Jinxiao Yang | 2021 | Molecular Plant2021，14，2： | 4 |
| 7 | CAS FGOALS-f3-L Model Datasets for CMIP6 GMMIP Tier-1 and Tier-3 Experiments显示文摘The Chinese Academy of Sciences(CAS)Flexible Global Ocean Atmosphere Land System(FGOALS-f3-L)model datasets prepared for the sixth phase of the Coupled Model Intercomparison Project(CMIP6)Global Monsoons Model Intercomparison Project(GMMIP)Tier-1 and Tier-3 experiments are introduced in this paper,and the model descriptions,experimental design and model outputs are demonstrated.There are three simulations in Tier-1,with different initial states,and five simulations in Tier-3,with different topographies or surface thermal status.Specifically,Tier-3 contains four orographic perturbation experiments that remove the Tibetan Iranian Plateau,East African and Arabian Peninsula highlands,Sierra Madre,and Andes,and one thermal perturbation experiment that removes the surface sensible heating over the Tibetan Iranian Plateau and surrounding regions at altitudes above 500 m.These datasets will contribute to CMIP6’s value as a benchmark to evaluate the importance of long-term and short-term trends of the sea surface temperature in monsoon circulations and precipitation,and to a better understanding of the orographic impact on the global monsoon system over highlands. | Bian HE Yimin LIU Guoxiong WU Qing BAO Tianjun ZHOU Xiaofei WU Lei WANG Jiandong LI Xiaocong WANG Jinxiao LI Wenting HU Xiaoqi ZHANG Chen SHENG and Yiqiong TANG | 2020 | Advances in Atmospheric Sciences2020，37，1： | 4 |
| 8 | Bioaccumulation, subcellular distribution and chemical forms of yttrium in rice seedling显示文摘Yttrium(Y) accumulation in soil is a serious environment problem in China. To understand the mechanisms involved in Y tolerance and detoxification in rice seedling, the bioaccumulation, subcellular distribution, and chemical forms of Y was investigated in the present study. The results show that the content of Y both in roots and shoots increases with the increasing concentration of Y, and a large amount of Y is stored in roots. Subcellular distribution of Y in rice indicates that the majority of Y is bound to cell wall. Meanwhile, various chemical forms of Y is absorbed by rice. The greatest amount of Y is extracted by 2% hydrate acetic acid(HAc) and 0.6 mol/L HCl(particularly 2% HAc), which indicates that most of Y is combined with un-dissolved phosphate and oxalate(particularly phosphate). Cluster analysis, based on Y abundance levels, reveals the relationship between Y distribution patterns and chemical forms. Our results imply that Y forming of precipitates with phosphate(and/or oxalate) and depositing in the cell wall may be a key strategy for Y detoxicity and tolerance in rice. | Shengnan Zheng Caiyun Zhang Kailun Shi Jinxiao Wang Guanjun Sun Qiaochu Hu Fengyun Zhao Xue Wang | 2018 | Journal of Rare Earths2018，36，3： | 4 |
| 9 | Developing high-efficiency base editors by combining optimized synergistic core components with new types of nuclear localization signal peptide显示文摘The clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein(Cas) system has been widely used for genome editing. In this system, the cytosine base editor(CBE) and adenine base editor(ABE) allow generating precise and irreversible base mutations in a programmable manner and have been used in many different types of cells and organisms. However, their applications are limited by low editing efficiency at certain genomic target sites or at specific target cytosine(C) or adenine(A) residues. Using a strategy of combining optimized synergistic core components, we developed a new multiplex super-assembled ABE(sABE) in rice that showed higher base-editing efficiency than previously developed ABEs. We also designed a new type of nuclear localization signal(NLS) comprising a FLAG epitope tag with four copies of a codon-optimized NLS(F4NLS^(r2)) to generate another ABE named F4NLS-sABE. This new NLS increased editing efficiency or edited additional A at several target sites. A new multiplex super-assembled CBE(sCBE) and F4NLS^(r2) involved F4NLS-sCBE were also created using the same strategy. F4NLS-sCBE was proven to be much more efficient than sCBE in rice. These optimized base editors will serve as powerful genome-editing tools for basic research or molecular breeding in rice and will provide a reference for the development of superior editing tools for other plants or animals. | Feipeng Wang Chengwei Zhang Wen Xu Shuang Yuan Jinling Song Lu Li Jiuran Zhao Jinxiao Yang | 2020 | The Crop Journal2020，8，3： | 3 |
| 10 | Comparisons of the temperature and humidity profiles of reanalysis products with shipboard GPS sounding measurements obtained during the 2018 Eastern Indian Ocean Open Cruise显示文摘本研究利用2018年春季东印度洋科学考察航次采集的GPS探空数据集,分析了三套再分析资料(JRA-55、MERR2和FGOALS-f2)对赤道东印度洋对流层中低层大气温湿廓线的模拟能力。结果表明:JRA-55和MERRA2能合理地再现850-600hPa之间的温度廓线的特征,最大偏差小于0.5度,但在850hPa以下的偏差增大,最底层的偏差分别达到了2和1.5度;两套再分析资料对850-600hPa之间的湿度扩线也具备较好的再现能力,但在850hPa以下JRA55和MERRA2再分析资料高估了相对湿度,最大湿度偏差可达15%。相对而言,FGOALS-f2分析资料对温度廓线再现能力最好,边界层内的温度偏差明显小于其它两套再分析资料;但FGOALS-f2也存在整层偏湿的问题,最大偏差在600hPa附近,误差接近20%。 | WANG Lei BAO Qing LI Jinxiao WANG Dongxiao LIU Yimin WU Guoxiong WU Xiaofei | 2019 | Atmospheric and Oceanic Science Letters2019，12，3： | 3 |
| 11 | Corrosion resistance and electrical conductivity of V/Ce conversion coating on magnesium alloy AZ31B显示文摘A V/Ce conversion coating was deposited in the surface of AZ31B magnesium alloy in a solution containing vanadate and cerium nitrate.The coating composition and morphology were examined.The conversion coating appears to consist of a thin and cracked coating with a scattering of spherical particles.The corrosion behavior of the substrate and conversion coating was studied by potentiodynamic polarization and electrochemical impedance spectroscopy (EIS).Compared with AZ31B magnesium alloy,the corrosion current density of the conversion coating is decreased by two orders of magnitude.The total impedance of the V/Ce conversion coating rise to 1.6×10^(3)Ω·cm^(2)in contrast with2.2×10^(2)Ω·cm^(2)of the bare AZ31B.In addition,the electrical conductivity of the coating was assessed by conductivity meter and Mott-Schottky measurement.The results reveal a high dependence of the conductivity of the coating on the semiconductor properties of the phase compositions. | Jinxiao Yang Xudong Wang Yiren Cai Xiuyu Yang | 2023 | International Journal of Minerals,Metallurgy and Materials2023，30，4： | 2 |
| 12 | Highly efficient CRISPR-SaKKH tools for plant multiplex cytosine base editing显示文摘Base editing, as an expanded clustered regularly interspaced short palindromic repeats(CRISPR)-Cas genome editing strategy, permits precise and irreversible nucleotide conversion. SaKKH, an efficient variant of a Cas9 ortholog from Staphylococcus aureus(SaCas9), is important in genome editing because it can edit sites with HHHAAT protospacer adjacent motif(PAM) that the canonical Streptococcus pyogenes Cas9(SpCas9) or its variants(e.g. xCas9, Cas9-NG) cannot. However, several technical parameters of SaKKH involved base editors have not been well defined and this uncertainty limits their application. We developed an effective multiplex cytosine base editor(SaKKHn-pBE) and showed that it recognized NNARRT, NNCRRT, NNGRGT, and NNTRGT PAMs. Based on 27 targets tested, we defined technical parameters of SaKKHn-pBE including the editing window, the preferred sequence context, and the mutation type. The editing efficiency was further improved by modification of the SaKKH sgRNA. These advances can be applied in future research and molecular breeding in rice and other plants. | Chengwei Zhang Feipeng Wang Si Zhao Guiting Kang Jinling Song Lu Li Jinxiao Yang | 2020 | The Crop Journal2020，8，3： | 1 |
| 13 | USP22 promotes the G1/S phase transition by upregulating FoxM1 expressionvia β-catenin nuclear localization and is associated with poor prognosis in stageII pancreatic ductal adenocarcinoma显示文摘 | Zhen Ning Aman Wang Jinxiao Liang Yunpeng Xie Jiwei Liu Lu Feng Qiu Yan Zhongyu Wang | 2014 | International Journal of Oncology2014，，4： | 1 |
| 14 | USP22 promotes epithelial-mesenchymal transition via the FAK pathwayin pancreatic cancer cells显示文摘 | Zhen Ning Aman Wang Jinxiao Liang Yunpeng Xie Jiwei Liu Qiu Yan Zhongyu Wang | 2014 | Oncology Reports2014，，4： | 1 |
| 15 | Ordered-Subsets Ridge Regression in Image Reconstruction 显示文摘 | Jinxiao Pan Yan Han Liping Liu Tie Zhou | | Journal of0，，： | 1 |
| 16 | Research and implemen tation of accss rights control in web systems 显示文摘 | LU Jinxiao MA Suxia QI Linhai | 2008 | Computer Engineering and Deign2008，29，10： | 1 |
| 17 | FGOALS-f3-L全球陆地降水气候变率评估:GMMIP与historical试验的比较显示文摘全球降水对人们生产生活和经济发展都至关重要。因此,评估模式对降水的模拟能力至关重要。近期大气物理研究所发布了两套CMIP6试验数据,结合GPCC降水资料,本文评估了两组试验对1900-2014年全球陆地降水以及各半球夏季降水模拟性能。结果表明,无论是年际尺度还是长期趋势的模拟,GMMIP试验均表现出更好的模拟性能。考虑到两组试验设计的角度和海温对年际降水的重要影响,本文探讨了全球海温异常尤其是ENSO事件对全球降水和各半球夏季降水的重要性。结果表明,赤道太平洋地区海温对降水模拟影响显著,其影响主要集中在中低纬地区。这对降水模拟的改进有很大帮助。 | TANG Yiqiong HE Bian BAO Qing LIU Yimin LI Jinxiao WU Guoxiong | 2020 | Atmospheric and Oceanic Science Letters2020，13，6： | 1 |
| 18 | Evaluation of thermal, thermal-alkaline, alkaline and electrochemical pretr- eatments on sludge to enhance anaerobic biogas production 显示文摘 | Xu Jiaobo Yuan Hai ping Lin Jinxiao | 2014 | Journal of the Taiwan Institute of Chemical Engineers2014，45，5： | 1 |
| 19 | 大气所全球25 km气候模式CAS FGOALS-f3-H参加CMIP6 HighResMIP数据介绍显示文摘高分辨率模式比较计划(HighResMIP)是CMIP6中新增的模式间比较计划,旨在关注水平分辨率对气候模拟的影响。本文介绍了中国科学院大气物理研究所全球25km FGOALS-f3-H和100km FGOALS-f3-L模式的基本信息,以及参与HighResMIP的试验设计,强迫数据,数据后处理方法等信息。水平分辨率25km的FGOALS-f3-H模式和100km FGOALS-f3-L模式由标准外强迫场驱动,且分别在1950-2014和2015-50时段进行两组协同试验,试验编号分别为'highresSST-present'及'highresSST-future'。模拟和预估数据集包含多种输出频率:1小时平均,3小时平均,6小时瞬时,日平均和月平均等。评估结果表明:水平分辨率的提高能够显著改进模式在热带气旋、日变化和极端降水的模拟性能。相较于全球100km低分辨率的FGOALS-f3-L,全球25km的FGOALS-f3-H能够更好的刻画热带气旋的生命周期和全球降水的日变化主要特征,特别是能够准确再现亚洲夏季风的降水日变化峰值时间。该数据集的发布将大大促进水平分辨率对气候模拟和预估影响的相关研究。 | BAO Qing LIU Yimin WU Guoxiong HE Bian LI Jinxiao WANG Lei WU Xiaofei CHEN Kangjun WANG Xiaocong YANG Jing ZHANG Xiaoqi | 2020 | Atmospheric and Oceanic Science Letters2020，13，6： | 1 |
| 20 | CAS FGOALS-f3-L Large-ensemble Simulations for the CMIP6 Polar Amplification Model Intercomparison Project显示文摘Large-ensemble simulations of the atmosphere-only time-slice experiments for the Polar Amplification Model Intercomparison Project(PAMIP)were carried out by the model group of the Chinese Academy of Sciences(CAS)Flexible Global Ocean-Atmosphere-Land System(FGOALS-f3-L).Eight groups of experiments forced by different combinations of the sea surface temperature(SST)and sea ice concentration(SIC)for pre-industrial,present-day,and future conditions were performed and published.The time-lag method was used to generate the 100 ensemble members,with each member integrating from 1 April 2000 to 30 June 2001 and the first two months as the spin-up period.The basic model responses of the surface air temperature(SAT)and precipitation were documented.The results indicate that Arctic amplification is mainly caused by Arctic SIC forcing changes.The SAT responses to the Arctic SIC decrease alone show an obvious increase over high latitudes,which is similar to the results from the combined forcing of SST and SIC.However,the change in global precipitation is dominated by the changes in the global SST rather than SIC,partly because tropical precipitation is mainly driven by local SST changes.The uncertainty of the model responses was also investigated through the analysis of the large-ensemble members.The relative roles of SST and SIC,together with their combined influence on Arctic amplification,are also discussed.All of these model datasets will contribute to PAMIP multi-model analysis and improve the understanding of polar amplification. | Bian HE Xiaoqi ZHANG Anmin DUAN Qing BAO Yimin LIU Wenting HU Jinxiao LI Guoxiong WU | 2021 | Advances in Atmospheric Sciences2021，38，6： | 1 |
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