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| 1 | Versatile Nucleotides Substitution in Plant Using an Improved Prime Editing System显示文摘Dear Editor,Base editors(BEs)based on the CRISPR/Cas9 system,including cytosine base editors and adenine base editors,which can efficiently perform four transition mutations(C·G-to-T·A and A·T-to-G·C),have been well studied and widely used to produce base mutations in a variety of organisms,including in plants such as rice. | Wen Xu Chengwei Zhang Yongxing Yang Si Zhao Guiting Kang Xiaoqing He Jinling Song Jinxiao Yang | 2020 | Molecular Plant2020，13，5： | 20 |
| 2 | Discriminated sgRNAs-Based SurroGate System Greatly Enhances the Screening Efficiency of Plant Base-Edited Cells显示文摘The development of CRISPR/Cas9-mediated base editing has made genomic modification more efficient. However, selection of genetically modified cells from millions of treated cells, especially plant cells, is still challenging. In this study, an efficient surrogate reporter system based on a defective hygromycin resistance gene was established in rice to enrich base-edited cells. After step-by-step optimization, the Discriminated sgRNAs-based SurroGate system (DisSUGs) was established by artificially differentiating the editing abilities of a wild-type single guide RNA (sgRNA) targeting the surrogate reporter gene and an enhanced sgRNA targeting endogenous sites. The DisSUGs enhanced the efficiency of screening base-edited cells by 3- to 5-fold for a PmCDA1-based cytosine-to-tyrosine base editor (PCBE), and 2.5- to 6.5-fold for an adenine base editor (ABE) at endogenous targets. These targets showed editing efficiencies of <25% in the conventional systems. The DisSUGs greatly enhanced the frequency of homozygous substitutions and expanded the activity window slightly for both a PCBE and an ABE. Analyses of the total number of single-nucleotide variants from whole-genome sequencing revealed that, compared with the no-enrichment PCBE strategy, the DisSUGs did not alter the frequency of genome-wide sgRNA-independent off-target mutations, but slightly increased the frequency of target-dependent off-target mutations. Collectively, the DisSUGs developed in this study greatly enhances the efficiency of screening plant base-edited cells and will be a useful system in future applications. | Wen Xu Yongxing Yang Ya Liu Guiting Kang Feipeng Wang Lu Li Xinxin Lv Si Zhao Shuang Yuan Jinling Song Ying Wu Feng Feng Xiaoqing He Chengwei Zhang Wei Song Jiuran Zhao Jinxiao Yang | 2020 | Molecular Plant2020，13，1： | 8 |
| 3 | Microstructure and Mechanical Properties of 45 vol.%SiC_p/7075Al Composite显示文摘Microstructure and mechanical behavior of high volume content Si Cp/7xxx Al composites have not been explored yet. Therefore, in the present work, 45 vol.% SiC p/7075 Al composite has been prepared by pressure infiltration method. High density dislocations were found around Si C/Al interface in SiC p/7075 Al composite after water-quenching and aging treatment. Fine dispersed nano-η′ phases were observed after the aging treatment. Adverse to other Si Cp/Al composites prepared by the pressure infiltration method,an interface layer was observed between SiC particles and Al matrix. Furthermore, high-resolution transmission electron microscopy(HRTEM) observation indicated that this interface layer was coherent/semicoherent with that of the SiC particles. 45 vol.% SiC p/7075 Al composite demonstrated high tensile strength(630 MPa) and micro-ductility. Compared to aged Si Cp/2024 Al composite, the aged Si Cp/7075 Al composite showed an increase of about 200% in the tensile strain and 90% in the tensile strength, respectively.It is speculated that nano-η′ phases in the Al matrix significantly contributed to the strengthening effect while the interface layer between Si C and Al matrix might be beneficial to the strength and plasticity of Si Cp/7075 Al composite. | Ziyang Xiu Wenshu Yang Ronghua Dong Murid Hussain Longtao Jiang YongXing Liu Gaohui Wu | 2015 | Journal of Materials Science & Technology2015，31，9： | 7 |
| 4 | Suppression of murine breast cancer metastasis by selective inhibition of CXCR4 by synthetic polypeptide derived from viral macrophage inflammatory protein Ⅱ显示文摘Stromal cell-derived factor-1 and its receptor CXC chemokine receptor-4(CXCR4)have been implicated in breast cancer metastasis.A significant association between HER2 and CXCR4 expression has been observed in human breast tumor tissues,and overexpression of CXCR4 is essential for HER2-mediated tumor metastasis.Moreover,CXCR4 expression is low in normal breast tissues and high in malignant tumors,suggesting that a blockade of CXCR4 may limit tumor metastasis.The present study investigated the action of a synthetic antagonist 21-mer peptide derived from viral macrophage inflammatory protein Ⅱ against CXCR4(NT21MP)in inhibiting metastasis in vitro and in vivo.The results showed that chemotaxis of SKBR3 cells toward SDF-1αwas reduced by NT21MP in a dose-dependent manner(P<0.05).NT21MP inhibited tumor growth at 500μg/kg and in combination with Herceptin,the anti-HER2 antibody.The in vivo metastatic assay showed that NT21MP significantly inhibited pulmonary metastasis,and the number of metastatic tumor nodes on the surface of the lung was greatly decreased.Compared with the saline-treated control group,PCNA expression was dose-dependently decreased by NT21MP,the percentage of apoptotic cells was increased,and CXCR4 mRNA and protein expression were downregulated.In conclusion,NT21MP inhibits cellular proliferation,promotes apoptosis by downregulating CXCR4 expression,and suppresses the progression of primary and metastatic tumors.CXCR4 may be a useful therapeutic target for breast cancer,and NT21MP may serve as a potential target drug for the treatment of breast cancer metastasis. | YANG QingLin DING YongXing CHEN ChangJie TANG Jie ZHANG Ju YANG ZhiFeng | 2010 | Chinese Science Bulletin2010，55，20： | 6 |
| 5 | Mapping stripe rust resistance genes by BSR-Seq:YrMM58 and YrHY1 on chromosome 2AS in Chinese wheat lines Mengmai 58 and Huaiyang 1 are Yr17显示文摘Stripe rust(yellow rust), caused by Puccinia striiformis f. sp. tritici(PST),is one of the most devastating fungal diseases in common wheat(Triticum aestivum L.) in China and worldwide. Resistance breeding is the most effective strategy to control diseases in crop plants. Chinese wheat lines Mengmai 58 and Huaiyang 1 are highly resistant to PST race CYR34(V26) at the adult plant stage. To genetically map the underlying resistance genes we developed segregating populations by crossing Mengmai 58 and Huaiyang 1 with the susceptible cultivar Nongda 399. The stripe rust resistances in Mengmai 58 and Huaiyang 1 were both controlled by single dominant genes, provisionally designated YrMM58 and YrHY1, respectively. Bulked segregant RNA-Seq(BSR-Seq) analysis showed that YrMM58 and YrHY1 were located in the same distal ~16 Mb region on chromosome 2 AS.Comparative genomics analysis with the physical map of Aegilops tauschii proved useful for developing additional markers to saturate the genetic linkage map. YrMM58 and YrHY1 were mapped to the distal end of chromosome arm 2 AS, with the closest marker WGGB148 being 7.7 cM and 3.8 cM from the resistance gene, which was considered to be Yr17. These markers can be used in marker-assisted selection. | Yong Wang Huaizhi Zhang Jingzhong Xie Bingmin Guo Yongxing Chen Huaiyu Zhang Ping Lu Qiuhong Wu Miaomiao Li Deyun Zhang Guanghao Guo Jian Yang Panpan Zhang Yan Zhang Xicheng Wang Hong Zhao Tingjie Cao Zhiyong Liu | 2018 | The Crop Journal2018，6，1： | 5 |
| 6 | High-speed visual target tracking with mixed rotation invariant description and skipping searching显示文摘This paper proposes a novel high-speed visual target tracking system based on mixed rotation invariant description(MRID) and skipping searching method. MRID is a novel rotation invariant description of texture and edge information by annular histograms and dominant direction. It overcomes rotation variant and large computation issues in conventional LBP-HOG feature description. The skipping searching method used in tracking can remarkably decrease the computation time by avoiding repeated searching operations.The proposed tracking system contains an image sensor, a hierarchical vision processor and an actuator with2 dimensions of freedom(DOF). The vision processor integrates processors with pixel-and row-level parallelism to speed up the tracking algorithm. Experiment results show that the proposed system can achieve over 1000-fps processing speed of the tracking algorithm under 750 × 480 resolution image. | Yongxing YANG Jie YANG Zhongxing ZHANG Liyuan LIU Nanjian WU | 2017 | Science China(Information Sciences)2017，60，6： | 5 |
| 7 | Mapping a leaf senescence gene els1 by BSR-Seq in common wheat显示文摘Leaf senescence is normally the last stage of plant development. Early senescence of functional leaves significantly reduces the photosynthetic time and efficiency, seriously affecting grain yield and quality in wheat. Discovering genes responsible for early leaf senescence(els) are necessary for developing novel germplasms and cultivars with delayed leaf-senescence through molecular manipulation and marker assisted selection. In this study, we identified an early leaf senescence line M114 in a derivative of a wheat breeding population. Genetic analysis indicated that early leaf senescence in M114 is controlled by a single recessive gene, provisionally designated els1. By applying bulked segregant analysis and RNA-Seq(BSR-Seq), seven polymorphic markers linked to els1 were developed and the gene was located on chromosome arm 2 BS in a 1.5 c M genetic interval between markers WGGB303 and WGGB305. A co-segregating marker, WGGB302, provide a starting point for fine mapping and map-based cloning of els1. | Miaomiao Li Beibei Li Guanghao Guo Yongxing Chen Jingzhong Xie Ping Lu Qiuhong Wu Deyun Zhang Huaizhi Zhang Jian Yang Panpan Zhang Yan Zhang Zhiyong Liu | 2018 | The Crop Journal2018，6，3： | 3 |
| 8 | The Significance of CXCR4 Expression for the Prediction of Lymph Node Metastasis in Breast Cancer Patients显示文摘OBJECTIVE The chemokine receptor(CXCR4)CXC chemokine receptor 4)plays an important role in cancer metastasis.We therefore studied differential expression of the CXCR4,as well as that of the biomarker HER2,so as to evaluate whether these biomarkers can be used to predict axillary lymph node metastasis in breast cancer patients. METHODS Immunohistochemistry was used to evaluate the CXCR4 and HER2 expressions and to examine the paraffin sections of the breast cancers at various stages.Positive lymph node expression was found in 80 of the cases,and in 7 there was negative expression. RESULTS Compared to the cases with negative lymph nodes, there was a high expression of CXCR4(26.3% vs.14.3%,P=0.013), and an over-expression of HER2(28.8% vs.14.3%,P=0.011). Moreover,there was a direct correlation between the CXCR4 and HER2 expressions and the tumor staging(P=0.000)and lymph node metastasis(P=0.032).When the two biomarkers,i.e.CXCR4 and HER2,were concurrently labeled,a high expression of one of the biomarkers could be seen in the cases with positive lymph nodes(51.3% vs.28.6%,P<0.003). CONCLUSION The chemokine receptor,CXCR4,is a new-type biomarker in predicting axillary lymph-node metastasis in breast cancers.Compared with the other markers,such as HER2 etc., assessment of CXCR4 can improve the prediction of the presence and extent of lymph node involvement. | Yongxing Ding Chenghua Li Qingling Yang Changjie Chen Zenong Cheng | 2008 | Chinese Journal of Clinical Oncology2008，5，2： | 3 |
| 9 | High efficient catalytic oxidation of 5-hydroxymethylfurfural into 2,5-furandicarboxylic acid under benign conditions with nitrogen-doped graphene encapsulated Cu nanoparticles显示文摘Selective oxidation of 5-hydroxymethylfurfual(HMF) to 2,5-furandicarboxylic acid(FDCA) as a bioplastics monomer is efficiently promoted by a simple system without noble-metal and base additives. In this work, graphene oxide(GO) was first synthesised by an electrochemical method with flexible graphite paper(FGP) as start carbon material, then, nitrogen-doped graphene(NG) layers encapsulated Cu nanoparticles(NPs) was prepared by one-step thermal treatment of GO supported Cu2+ in flowing NH3 atmosphere. Compared with NG supported Cu NPs prepared by the traditional impregnation method, enhanced catalytic activity was achieved over Cu/NG and an FDCA yield of 95.2% was achieved under mild reaction conditions with tert-butylhydroperoxide(t-BuOOH) as the oxidant. Control experiments with different catalysts and different addition procedure of t-BuOOH showed the yield of HMF and various intermediates during reaction. From the changing of intermediates concentrations and reaction rates, a reaction pathway through HMF-DFF-FFCA-FDCA was proposed. This work gives a more convenient, more green,more economical and effective method in encapsulated metal NPs preparation and high selectivity in HMF oxidation to FDCA under mild conditions. | Chaoxin Yang Xiao Li Zhenzhou Zhang Bohan Lv Jiachun Li Zhenjian Liu Wanzhen Zhu Furong Tao Guangqiang Lv Yongxing Yang | 2020 | Journal of Energy Chemistry2020，29，11： | 3 |
| 10 | A novel telomerase-interacting OTU protein of Eimeria tenella and its telomerase-regulating activity显示文摘 | Pu Wang Weirong Wang Ju Yang Yongxing Ai Pengtao Gong Xichen Zhang | 2017 | Acta Biochimica et Biophysica Sinica2017，49，8： | 2 |
| 11 | Mitochondrial transcription factor A plays opposite roles in the initiation and progression of colitis-associated cancer显示文摘Background:Mitochondria are key regulators in cell proliferation and apoptosis.Alterations in mitochondrial function are closely associated with inflammation and tumorigenesis.This study aimed to investigate whether mitochondrial transcription factor A(TFAM),a key regulator of mitochondrial DNA transcription and replication,is involved in the initiation and progression of colitis-associated cancer(CAC).Methods:TFAM expression was examined in tissue samples of inflammatory bowel diseases(IBD)and CAC by immunohistochemistry.Intestinal epithelial cell(IEC)-specific TFAM-knockout mice(TFAM^(△IEC))and colorectal cancer(CRC)cells with TFAM knockdown or overexpression were used to evaluate the role of TFAMin colitis and the initiation and progression ofCAC.The underlying mechanisms of TFAMwere also explored by analyzingmitochondrial respiration function and biogenesis.Results:The expression of TFAM was downregulated in active IBD and negatively associated with the disease activity.The downregulation of TFAM in IECs was induced by interleukin-6 in a signal transducer and activator of transcription 3(STAT3)/miR-23b-dependent manner.In addition,TFAM knockout impaired IECturnover to promote dextran sulfate sodium(DSS)-induced colitis inmice.Of note,TFAMknockout increased the susceptibility of mice to azoxymethane/DSSinduced CAC and TFAM overexpression protected mice from intestinal inflammation and colitis-associated tumorigenesis.By contrast,TFAM expression was upregulated in CAC tissues and contributed to cell growth.Furthermore,it was demonstrated that β-catenin induced the upregulation of TFAM through c-Myc in CRC cells.Mechanistically,TFAMpromoted the proliferation of both IECs and CRC cells by increasing mitochondrial biogenesis and activity.Conclusions:TFAM plays a dual role in the initiation and progression of CAC,providing a novel understanding of CAC pathogenesis. | Shirong Yang Xianli He Jing Zhao Dalin Wang Shanshan Guo Tian Gao Gang Wang Chao Jin Zeyu Yan Nan Wang Yongxing Wang Yilin Zhao Jinliang Xing Qichao Huang | 2021 | Cancer Communications2021，41，8： | 2 |
| 12 | The Pellet Enhanced Performance on the HL-2A Tokamak显示文摘 | DING Xuantong YANG Qingwei YAN Longwen ZHU Genliang CAO Zeng XIAO Zhenggui GAO Qingdi LONG Yongxing LIU Dequan LIU Yi ZHOU Yan PAN Yudong CUI Zhengying HUANG Yuan LIU Zetian SHI Zhongbing JI Xiaoquan XIAO Weiwen LIU Yong | 2006 | Southwestern Institute of Physics Annual Report2006，，1： | 1 |
| 13 | Ultra-deep desulfurization of diesel by selective oxidation with [C 18 H 37 N(CH 3 ) 3 ] 4 [H 2 NaPW 10 O 36 ] catalyst assembled in emulsion droplets显示文摘 | Hongying Lü Jinbo Gao Zongxuan Jiang Fei Jing Yongxing Yang Gang Wang Can Li | 2006 | Journal of Catalysis2006，，2： | 1 |
| 14 | Deep desulfurization from fuel oil via selective oxidation using an amphiphilic peroxotungsten catalyst assembled in emulsion droplets显示文摘 | Jinbo Gao Shouguo Wang Zongxuan Jiang Hongying Lu Yongxing Yang Fei Jing Can Li | 2006 | Journal of Molecular Catalysis A Chemical2006，，1： | 1 |
| 15 | Selective Oxidations on Recoverable Catalysts Assembled in Emulsions显示文摘 | Can Li Jinbo Gao Zongxuan Jiang Shouguo Wang Hongying Lu Yongxing Yang Fei Jing | 2005 | Topics in Catalysis (-)2005，，12： | 1 |
| 16 | High-speed target tracking system based on multi-interconnection heterogeneous processor and multi-descriptor algorithm显示文摘Dear editor,Target tracking has been a subject of intense scholarly research in past decades. Many parallel processors [1–4] and efficient tracking algorithms based on feature extraction [3–5] have been proposed to meet the requirements of high-speed tracking. However. | Jiaqing WANG Yongxing YANG Liyuan LIU Nanjian WU | 2019 | Science China(Information Sciences)2019，62，6： | 1 |
| 17 | Dibenzothiophene hydrodesulfurization activity and surface sites of silica-supported MoP, Ni 2 P, and Ni<ce:glyph name='sbnd'/>Mo<ce:glyph name='sbnd'/>P catalysts显示文摘 | Fuxia Sun Weicheng Wu Zili Wu Jun Guo Zhaobin Wei Yongxing Yang Zongxuan Jiang Fuping Tian Can Li | 2004 | Journal of Catalysis2004，，2： | 1 |
| 18 | Ultra-deep desulfurization of diesel by selective oxidation with [C 18 H 37 N(CH 3 ) 3 ] 4 [H 2 NaPW 10 O 36 ] catalyst assembled in emulsion droplets显示文摘 | Hongying Lü Jinbo Gao Zongxuan Jiang Fei Jing Yongxing Yang Gang Wang Can Li | 2006 | Journal of Catalysis2006，，2： | 1 |
| 19 | Developing an efficient and visible prime editing system to restore tobacco 8-hydroxy-copalyl diphosphate gene for labdane diterpene Z-abienol biosynthesis显示文摘Prime editing(PE)is a versatile CRISPR-Cas based precise genome-editing platform widely used to introduce a range of possible base conversions in various organisms.However,no PE systems have been shown to induce heritable mutations in tobacco,nor in any other dicot.In this study,we generated an efficient PE system in tobacco that not only introduced heritable mutations,but also enabled anthocyanin-based reporter selection of transgene-free T_(1) plants.This system was used to confer Zabienol biosynthesis in the allotetraploid tobacco cultivar HHDJY by restoring a G>T conversion in the NtCPS2 gene.High levels of Z-abienol were detected in the leaves of homozygous T_(1) plants at two weeks after topping.This study describes an advance in PE systems and expands genome-editing toolbox in tobacco,even in dicots,for use in basic research and molecular breeding.And restoring biosynthesis of Z-abienol in tobacco might provide an efficient way to obtain Z-abienol in plants. | Jianduo Zhang Lu Zhang Chengwei Zhang Yongxing Yang Huayin Liu Lu Li Shengxue Zhang Xianggan Li Xinxiang Liu Ya Liu Jin Wang Guangyu Yang Qingyou Xia Weiguang Wang Jinxiao Yang | 2023 | Science China(Life Sciences)2023，66，12： | 1 |
| 20 | A resazurin-based,nondestructive assay for monitoring cell proliferation during a scaffold-based 3D culture process显示文摘Development of viable cell estimation method without sacrificing proliferation and functions of cells cultured on regenerative biomaterials is essential for regenerative engineering.Cytotoxicity and depletion of resazurin are critical but often overlooked limitations that hindered applications of resazurin in viable cell estimation.The present work found that cytotoxicity and depletion of resazurin depended on cell concentration,resazurin concentration and resazurin incubation time.A simple strategy which only allowed cells to incubate with resazurin during each measurement was developed to eliminate negative effects of resazurin.This strategy was verified by monitoring proliferation of MC3T3-E1 preosteoblasts on poly(D,L-lactic acid)scaffold during a continuous 3D culture process for up to 21 days,comparing the accuracy with MTT assay which is a destructive assay with high sensitivity and accuracy and commonly used in regenerative engineering and comparing viability,proliferation and differentiation functions of MC3T3-E1,which were treated with/without this strategy for nondestructive evaluation.This method showed comparable linearity of standard curve and characteristics of growth curve to MTT assay.No major negative effects of this method on MC3T3-E1 viability and functions were found.Our work highlighted the importance of the concentration and incubation time of resazurin in designing application-specific nondestructive viability assay and would be helpful in improving the implanted medical devices as well as in regenerative engineering. | Xianghui Gong Zhuqing Liang Yongxing Yang Haifeng Liu Jing Ji Yubo Fan | 2020 | Regenerative Biomaterials2020，7，3： | 1 |
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