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| 1 | A seven-gene-deleted African swine fever virus is safe and effective as a live attenuated vaccine in pigs显示文摘African swine fever(ASF)is a devastating infectious disease in swine that is severely threatening the global pig industry.An efficacious vaccine is urgently required.Here,we used the Chinese ASFV HLJ/18 as a backbone and generated a series of genedeleted viruses.The virulence,immunogenicity,safety,and protective efficacy evaluation in specific-pathogen-free pigs,commercial pigs,and pregnant sows indicated that one virus,namely HLJ/18-7GD,which has seven genes deleted,is fully attenuated in pigs,cannot convert to the virulent strain,and provides complete protection of pigs against lethal ASFV challenge.Our study shows that HLJ/-18-7GD is a safe and effective vaccine against ASFV,and as such is expected to play an important role in controlling the spread of ASFV. | Weiye Chen Dongming Zhao Xijun He Renqiang Liu Zilong Wang Xianfeng Zhang Fang Li Dan Shan Hefeng Chen Jiwen Zhang Lulu Wang Zhiyuan Wen Xijun Wang Yuntao Guan Jinxiong Liu Zhigao Bu | 2020 | Science China(Life Sciences)2020，63，5： | 72 |
| 2 | Efficient replacement of long DNA fragments via non-homologous end joining at norrcoding regions显示文摘Targeted double-strand breaks(DSBs)in genomes can be introduced efficiently by endonucleases(Umov et al.,2010;Jinek et al.,2012;Joung and Sander,2013),including zinc-finger nucleases,tran scription activator-like effector nu cleases,and clustered regularly in terspaced palindromic repeats(CRISPR)/Cas9.After DSBs,DNA repair is mainly via homology-directed repair(HDR)and/or non-homologous end joining(NHEJ)(Hustedt and Durocher,2016).It was reported that genomic DNA replacement can be achieved via HDR at the site of DSBs in multiple organisms(Dickinson et al.,2013;Yang et al.,2013;Zu et al.,2013),but the efficiency is still not enough for general application,in particular for replacing long DNA fragment that is more than hundreds of base pairs(bps).As NHEJ is 10-fold more active than HDR at DSB sites(Mao et al.,2008),we speculated that NHEJ can be utilized to implement long genomic DNA replacement with high efficiency. | Shanye Gu Jia Li Siyuan Li Jianbin Cao Jiwen Bu Yonggang Ren Wenjie Du Zhecong Chen Chufan Xu Mingcang Wang Lai Jiang Cheng Huang Jiulin Du | 2021 | Journal of Molecular Cell Biology2021，13，1： | 0 |
| 3 | Establishment of an indirect immunofluorescence assay for the detection of African swine fever virus antibodies显示文摘African swine fever(ASF)continues to cause enormous economic loss to the global pig industry.Since there is no safe and effective vaccine,accurate and timely diagnosis of ASF is essential to implement control measures.Indirect immunofluorescence assay(IFA)is a gold standard serological method recommended by the World Organization for Animal Health(WOAH).In this study,we used primary fetal kidney cells to establish a wild boar cell line(BK2258)that supported the efficient replication of ASF virus(ASFV)SD/DY-I/21 and showed visible cytopathic effect(CPE).Moreover,using BK2258,we established a sensitive and specific IFA for ASFV antibody detection.To standardize and evaluate the performance of this assay,we used serum samples from pigs infected with the low virulent genotype I SD/DY-I/21 and genotype II HLJ/HRB1/20,and immunized with the vaccine candidate HLJ/18-7GD,field samples,and negative serum samples.The IFA reacted with the ASFV-positive sera and displayed bright fluorescence foci.There was no non-specific green fluorescence due to cellular senescence or other cell damage-causing factors.Compared to a commercial indirect enzyme-linked immunosorbent assay(iELISA),ASFV antibodies were detected 1–4 days earlier using our IFA.The detection limits of the IFA and iELISA for the same ASFV-antibody positive serum samples were 1:25,600 and 1:6,400,respectively,indicating that the IFA is more sensitive than iELISA.The newly established IFA was highly specific and did not cross-react with sera positive for six other important porcine pathogens(i.e.,Classical swine fever virus(CSFV),Porcine reproductive and respiratory syndrome virus(PRRSV),Porcme circovirus type 2(PCV2),Pseudorabies virus(PRV),Foot-and-Mouth disease virus type O(FMDV/O),and Porcine epidemic diarrhea virus(PEDV)).This study thus provides a sensitive,specific,and reliable detection method that is suitable for the serological diagnosis of ASF. | Wan Wang Zhenjiang Zhang Weldu Tesfagaber Jiwen Zhang Fang Li Encheng Sun Lijie Tang Zhigao Bu Yuanmao Zhu Dongming Zhao | 2024 | Journal of Integrative Agriculture2024，23，1： | 0 |
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