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您的检索式：作者名="Zhecong Chen"
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| 1 | Efficient replacement of long DNA fragments via non-homologous end joining at norrcoding regions显示文摘Targeted double-strand breaks(DSBs)in genomes can be introduced efficiently by endonucleases(Umov et al.,2010;Jinek et al.,2012;Joung and Sander,2013),including zinc-finger nucleases,tran scription activator-like effector nu cleases,and clustered regularly in terspaced palindromic repeats(CRISPR)/Cas9.After DSBs,DNA repair is mainly via homology-directed repair(HDR)and/or non-homologous end joining(NHEJ)(Hustedt and Durocher,2016).It was reported that genomic DNA replacement can be achieved via HDR at the site of DSBs in multiple organisms(Dickinson et al.,2013;Yang et al.,2013;Zu et al.,2013),but the efficiency is still not enough for general application,in particular for replacing long DNA fragment that is more than hundreds of base pairs(bps).As NHEJ is 10-fold more active than HDR at DSB sites(Mao et al.,2008),we speculated that NHEJ can be utilized to implement long genomic DNA replacement with high efficiency. | Shanye Gu Jia Li Siyuan Li Jianbin Cao Jiwen Bu Yonggang Ren Wenjie Du Zhecong Chen Chufan Xu Mingcang Wang Lai Jiang Cheng Huang Jiulin Du | 2021 | Journal of Molecular Cell Biology2021，13，1： | 0 |
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